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http://dx.doi.org/10.4217/OPR.2017.39.4.245

Associated Bacterial Community Structures with the Growth of the Marine Centric Diatom Cyclotella meneghiniana: Evidence in Culture Stages  

Choi, Won-Ji (Department of Biotechnology, Sangmyung University)
Park, Bum Soo (Department of Biotechnology, Sangmyung University)
Guo, Ruoyu (Department of Biotechnology, Sangmyung University)
Ki, Jang-Seu (Department of Biotechnology, Sangmyung University)
Publication Information
Ocean and Polar Research / v.39, no.4, 2017 , pp. 245-255 More about this Journal
Abstract
There are a number of pieces of evidences that suggest a link between marine diatoms and microorganisms, but knowledge about related microbial communities is greatly lacking. The present study investigated the microbial community structures related to the growth of the marine diatom Cyclotella meneghiniana. We collected free-living bacteria (FLB) and particle-associated bacteria (PAB) at each growth stage (e.g., lag, exponential, stationary and death) of the diatom, and analyzed their bacterial 16S rDNA using pyrosequencing. Metagenomics analysis showed that community structures of FLB and PAB differed considerably with the progress of growth stages. FLB showed higher diversity than PAB, but variation in the different growth stages of C. meneghiniana was more evident in PAB. The proportion of the genus Hoeflea, belonging to the order Rhizobiales, was dominant in both FLB and PAB, and it gradually increased with the growth of C. meneghiniana. However, Enhydrobacter clade tended to considerably decrease in PAB. In addition, Marinobacter decreased steadily in FLB, but first increased and then decreased in PAB. These results suggest that Hoeflea, Enhydrobacter, and Marinobacter may be closely related to the growth of diatom C. meneghiniana.
Keywords
Cyclotella meneghiniana; bacterial community structure; free-living bacteria; particle-associated bacteria;
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