• Journal of Physiology & Pathology in Korean Medicine
• /
• v.36 no.2
• /
• pp.66-72
• /
• 2022

Flos Lonicerae Japonicae (the flower buds of Lonicera japonica Thunberg) has been used as an antibacterial and antiviral drug in Korean Medicine. The aim of this study is to evaluate the effect of Flos Lonicerae Japonicae water extract (FL) on the production of cytokines in RAW 264.7 mouse macrophages stimulated by lipopolysaccharide (LPS). After 24 h treatment, the production of various cytokines from RAW 264.7 was measured with multiplex cytokine assay using Bio-Plex 200 suspension array system. FL at concentrations of 50, 100, and 200 ㎍/mL significantly inhibited productions of tumor necrosis factor-α, macrophage inflammatory protein (MIP)-1β, and MIP-2 in LPS-stimulated RAW 264.7 cells; FL at concentrations of 100 and 200 ㎍/mL significantly inhibited productions of leukemia inhibitory factor, LIX (CXCL5), and RANTES in LPS-stimulated RAW 264.7 cells; FL at concentrations of 200 ㎍/mL significantly inhibited productions of granulocyte-macrophage colony-stimulating factor and macrophage colony-stimulating factor in LPS-stimulated RAW 264.7 cells; FL at concentrations of 50 and 100 ㎍/mL significantly increased productions of interleukin (IL)-10 in LPS-stimulated RAW 264.7 cells; FL at concentrations of 50, 100, and 200 ㎍/mL significantly increased productions of IL-6 and interferon gamma-induced protein-10 in LPS-stimulated RAW 264.7 cells; FL at concentrations of 100 and 200 ㎍/mL significantly increased productions of monocyte chemoattractant protein-1 in LPS-stimulated RAW 264.7 cells. Taken together, these data mean that FL might modulate productions of cytokines, chemokines, and growth factor in LPS-stimulated macrophages. Further study needs to verify the exact mechanism for modulatory activities of FL with macrophages.

• New & Renewable Energy
• /
• v.5 no.1
• /
• pp.11-17
• /
• 2009

To improve the performance of the anode-supported Solid Oxide Fuel Cell (SOFC) which can be operated at an intermediate temperature, the functional layer (FL) is introduced on a anode substrate. And the scandia-stabilized zirconia (ScSZ) and samaria-doped ceria (SDC) which have higher ionic conductivity and better chemical stability than yttria-stabilized zirconia (YSZ) are used as material for the anode FL with the Ni, The fabrication process of anode-supported single cell with the anode FL was established and the power density of those was evaluated. As a result, the sample with anode FL (Ni-YSZ) has higher power density than normal cell. The single cell which was composed of the FL (Ni-YSZ) and electrolyte (YSZ) showed about $550mW/cm^2$ of the maximum power density at $650^{\circ}C$ and $1430mW/cm^2$ at $750^{\circ}C$ respectively, In case of the single cell using the ScSZ and SDC as anode FL, the performance of samples decreased rapidly and those showed unstable voltage during long-term test. In case of using methane as a fuel, the cell performance with each FL decreased comparing with $H_2$ fuel. In the region of a high current density, there are large concentration polarizations.

• Korean Journal of Organic Agriculture
• /
• v.24 no.1
• /
• pp.99-114
• /
• 2016

This study was conducted to evaluate effects of various eco light sources with various lighting distance in 'Viking' rose (Rosa spp.) on the growth and flowering quality to be applied for farm sites. Treatment included 10-, 20-, and 30-RL (-BL, -RBL, -FL, and -IL), which referred to red LED (blue LED, red+blue LED, fluorescent, and incandescent) lighting at 10 cm, 20 cm, 30 cm respectively, apart from flowers. NL referred to natural light as a control. Growth and flowering of 'Viking' rose were non-destructively measured at 4, 6, and 8 weeks after treatment (WAT). FL treatment increased plant height at 4, 6, and 8 WAT, regardless of lighting distance, with the shortest height observed for the NL-treated flowers. 30 RL treatment also increased plant height at 6 and 8 WAT. Stem diameter and number of leaves were not significantly different for all the treatments at 8 WAT, with the lowest values observed for RBL treated-flowers among the light source treatments. Number of root was the greatest for the 30 BL-treated flowers (10.0) but the fewest for the 30 FL (4.7). Length of flower neck at 6 WAT was the extended by 6~7 cm in the 10 FL and 20 FL treatments as well as by 5~6 cm in the 20 RL and 30 RL treatments, inducing 100% of flowering. NL increased $a^*$ (29) of flower color, with the lowest value (10) observed for 20 RL. All things considered, 30 RL would be the best interaction treatment of source and distance of eco light to improve plant height and flowering quality of 'Viking' rose.

• Nutrition Research and Practice
• /
• v.12 no.3
• /
• pp.199-207
• /
• 2018

BACKGROUND/OBJECTIVES: Fermented Laminaria japonica (FL), a type sea tangle used as a functional food ingredient, has been reported to possess cognitive improving properties that may aid in the treatment of common neurodegenerative disorders, such as dementia. MATERIALS/METHODS: We examined the effects of FL on scopolamine (Sco)- and ethanol (EtOH)-induced hippocampus-dependent memory impairment, using the Passive avoidance (PA) and Morris water maze (MWM) tests. To examine the underlying mechanisms associated with neuroprotective effects, we analyzed acetylcholine (ACh) and acetylcholinesterase (AChE) activity, brain tissue expression of muscarinic acetylcholine receptor (mAChR), cAMP response element binding protein (CREB) and extracellular signal-regulated kinases 1/2 (ERK1/2), and immunohistochemical analysis, in the hippocampus of mice, compared to current drug therapy intervention. Biochemical blood analysis was carried out to determine the effects of FL on alanine transaminase (ALT), aspartate transaminase (AST), and triglyceride (TG) and total cholesterol (TC) levels. 7 groups (n = 10) consisted of a control (CON), 3 Sco-induced dementia and 3 EtOH-induced dementia groups, with both dementia group types containing an untreated group (Sco and EtOH); a positive control, orally administered donepezil (Dpz) (4mg/kg) (Sco + Dpz and EtOH + Dpz); and an FL (50 mg/kg) treatment group (Sco + FL50 and EtOH + FL50), orally administered over the 4-week experimental period. RESULTS: FL50 significantly reduced EtOH-induced increase in AST and ALT levels. FL50 treatment reduced EtOH-impaired step-through latency time in the PA test, and Sco- and EtOH-induced dementia escape latency times in the MWM test. Moreover, anticholinergic effects of Sco and EtOH on the brain were reversed by FL50, through the attenuation of AChE activity and elevation of ACh concentration. FL50 elevated ERK1/2 protein expression and increased p-CREB (ser133) in hippocampus brain tissue, according to Western blot and immunohistochemistry analysis, respectively. CONCLUSION: Overall, these results suggest that FL may be considered an efficacious intervention for Sco- and EtOH-induced dementia, in terms of reversing cognitive impairment and neuroplastic dysfunction.

• Fisheries and Aquatic Sciences
• /
• v.1 no.2
• /
• pp.227-231
• /
• 1998

The teleosts represent ancient real-bony vertebrates in phylogeny and resemble major genetic patterns to higher vertebrates. In the present study, we have defined the single family of insulin-like growth factors (IGFs) from flounder (Paralichthys olivaceus), compared to the prototype of IGFs observed in the Agnathan hagfish. In flounder, IGFs are clearly diverged into two major types including type I and II, and they are structurally similar by displaying a multidomain structure consisting of five functional regions as previously found in other vertebrates. However, flIGF-I appears to be more basic (pI 8.03) than the flIGF-II (pI 5.34) in the fully processed form for the B to D domain region. The flIGF-I seems to contain an evolutionary conserved Asn-linked glycosylation in E domain, which is not found in flIGF­II. The most interesting feature is that flIGF-II appeared to be structurally close to hagfish IGF in secondary structures, particularly in Band D domains. This could tell us an idea on the molecular divergence of IGFs from the Agnatha to teleosts during the vertebrate phylogeny. It also support, in part, a notion regarding on how IGF-II is appeared as more embryonic during development. Nonetheless, the biologically active B to D domain region of flIGF-II shows significant sequence homology of $65.6\%$ to flIGF-Is and contains the evolutionary conserved insulin-family signature, as well as a reserved recognition site (Lys) in D domain, necessary to generate proteolytic cleavage for E-peptide. A significant structural difference was found in E domain in which flIGF-I possesses two potential alternative splicing donor site at $Val^{17,\;24}$ of E domain. Therefore, it seems so far that IGF-I sorely produces spliced variants due to the spliced E-peptide moiety while IGF-II appears to be maintained in a single type during evolution. IGF-II, however, may be also possible to transcribe unidentified variants, depending on the physiological conditions of tissues in vertebrates in vivo.

• Korean Journal of Ichthyology
• /
• v.21 no.2
• /
• pp.93-99
• /
• 2009

The maturation and spawning of Black seabream Acanthopagrus schlegeli were investigated using samples randomly collected in the Southern Sea of Korea from March 2007 to February 2008. The FL at 50% sex-changers maturity was estimated to be 26.7 cm. We estimated monthly changes in maturity stages and gonadosomatic index (GSI). The spawning period ranged from March to July, and the peak spawning occurred in May and June. The fecundity (F) was 839,600 eggs at 32.2 cm fork length (FL) to 2,894,913 eggs at 42.0 cm FL, and the relationship between FL and F was expressed as $F=1.264FL^{3.869}$ ($R^2=0.736$). The FL at 50% group maturity was estimated to be 25.8 cm for females and 19.0 cm for males. The sex ratio was 19.9% for female, 20.9% for early sex-changers, 28.7% late sex-changers, 30.5% for male.

• Analytical Science and Technology
• /
• v.32 no.3
• /
• pp.77-87
• /
• 2019

High viscosity carbon black dispersions are used in various industrial fields such as color cosmetics, rubber, tire, plastic and color filter ink. However, carbon black particles are unstable to heat due to inherent characteristics, and it is very difficult to keep the quality of the product constant due to agglomeration of particles. In general, particle size analysis is performed by dynamic light scattering (DLS) during the dispersion process in order to select the optimum dispersant in the carbon black dispersion process. However, the existing low viscosity analysis provides reproducible particle distribution analysis results, but it is difficult to select the optimum dispersant because it is difficult to analyze the reproducible particle distribution at high viscosity. In this study, dynamic light scattering (DLS) and asymmetrical flow field-flow fractionation (AsFlFFF) analysis methods were compared for reproducible particle size analysis of high viscosity carbon black. First, the stability of carbon black dispersion was investigated by particle size analysis by DLS and AsFlFFF according to milling time, and the validity of analytical method for the selection of the optimum dispersant useful for carbon black dispersion was confirmed. The correlation between color and particle size of particles in high viscosity carbon black dispersion was investigated by using colorimeter. The particle size distribution from AsFlFFF was consistent with the colorimetric results. As a result, the correlation between AsFlFFF and colorimetric results confirmed the possibility of a strong analytical method for determining the appropriate dispersant and milling time in high viscosity carbon black dispersions. In addition, for nanoparticles with relatively broad particle size distributions such as carbon black, AsFlFFF has been found to provide a more accurate particle size distribution than DLS. This is because AsFlFFF, unlike DLS, can analyze each fraction by separating particles by size.

• The Journal of Korean Institute of Information Technology
• /
• v.17 no.5
• /
• pp.13-20
• /
• 2019

Recently, object detection technology based on CNN has been actively studied. Object detection technology is used as an important technology in autonomous vehicles, intelligent image analysis, and so on. In this paper, we propose a rotation change robust object detection system by applying MI-FL (Moment Invariant-Feature Layer) to SSD (Single Shot Multibox Detector) which is one of CNN-based object detectors. First, the features of the input image are extracted based on the VGG network. Then, a total of six feature layers are applied to generate bounding boxes by predicting the location and type of object. We then use the NMS algorithm to get the bounding box that is the most likely object. Once an object bounding box has been determined, the invariant moment feature of the corresponding region is extracted using MI-FL, and stored and learned in advance. In the detection process, it is possible to detect the rotated image more robust than the conventional method by using the previously stored moment invariant feature information. The performance improvement of about 4 ~ 5% was confirmed by comparing SSD with existing SSD and MI-FL.

• Journal of Digital Convergence
• /
• v.19 no.1
• /
• pp.99-105
• /
• 2021

This study aimed to verify the positive effect of work meaningfulness(WM) on flourishing(FL) with the successive positive effects of work engagement(WE) and job performance(JP) as dual mediators. We conducted an online survey of 395 incumbents working in the companies of different types and statistically analyzed data using SPSS and SPSS Process macro for hierarchical regression and bootstrapping. The results are as follows: 1. correlation coefficients of all combinations from four variables are significant, 2. simple mediation effects(WM-WE-FL and WE-JP-FL) are significant, 3. dual mediation effet(WM-WE-JP-FL) is verified. This study showed practical guide for the efficient management of employees in recent autonomous work environment and further studies with longitudinal method will be needed in order to verify substantially the dual mediation.

• Korean Journal of Community Nutrition
• /
• v.28 no.6
• /
• pp.495-508
• /
• 2023

Objectives: This study aimed to develop a campus-based intervention program to enhance food literacy (FL) among university students. Methods: In the initial phase, we conducted a literature review of FL intervention studies and held in-depth interviews with university students to identify facilitators and barriers to improving and practicing FL. Expert counseling sessions were conducted with nutrition education, marketing, and service design professionals. The results of this phase led to the creation of an initial curriculum draft. In the second phase, a follow-up survey was conducted with young adults to assess the acceptability of the developed curriculum. After the follow-up survey, additional meetings were conducted with the aforementioned experts, and the curriculum was further refined based on their input. Results: An 11-week FL intervention program was devised using constructs from the Social Cognitive Theory. The weekly curriculum consisted of 90-min theory-based and 90-min hands-on experience sessions. Three primary aspects of FL were covered: nutrition and food safety, cultural and relational dimensions, and socio-ecological aspects. Program highlights included cooking sessions for crafting traditional Korean desserts, lectures on animal welfare, insights into zero-waste practices, and communal eating experiences. Based on the study team's previous research, the program also addressed mindful eating, helping participants understand the relationship with their eating habits, and providing strategies to manage negative emotions without resorting to food. Yoga sessions and local farm visits were incorporated into the curriculum to promote holistic well-being. Conclusions: This study elucidated the comprehensive process of creating a campus-based curriculum to enhance FL among university students, a group particularly susceptible to problematic eating behaviors and low FL levels. The developed program can serve as a blueprint for adaptation to other campuses seeking to bolster students' FL.