• Reproductive and Developmental Biology
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• v.32 no.1
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• pp.15-20
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• 2008

Pronuclear DNA microinjection has been the most universal method in transgenic animal production but its success rate of transgenesis in mammals are extremely low. To address this long-standing problem, we used retrovirus- and lentivirus-based vectors carrying the enhanced green fluorescent protein (EGFP) gene under the control of ubiquitously active cytomegalovirus (CMV) promoter to deliver transgenes to bovine embryos. The rate of transgenesis was evaluated by counting EGFP positive blastocysts after injection of concentrated virus stock into the perivitelline space of the bovine oocytes in metaphase II. Among two different types of lentivirus vectors derived from FIV (feline immunodeficiency virus) and HIV (human immunodeficiency virus), the former scored the higher gene transfer efficiency; almost 100% of the blastocysts developed from the oocytes infected with FIV-based vector were EGFP positive. As for the vectors derived Com HIV lentivirus, the transgenesis rate of the blastocysts was reduced to 39%.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2006.05a
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• pp.793-802
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• 2006

In this study, a fluid/structure coupled analysis system for simulating complex flow-induced vibration (FIV) phenomenon of cascades has been developed. The flow is modeled using Euler and Wavier-Stokes equations with different turbulent models. The fluid domains are modeled using the unstructured grid system with dynamic deformations due to the motion of structural boundary. The Spalart-Allmaras (S-A) and the SST ${\kappa}-{\omega}$ turbulent models are used to predict the transonic turbulent flows. A fully implicit time marching scheme based on the Newmark direct integration method is used in order to solve the coupled governing equations for viscous flow-induced vibration phenomena. For the purpose of validation for the developed FIV analysis system, comparison results for computational analyses of steady and unsteady aerodynamics and flutter analyses are presented in the transonic flow region. In addition, flow-induced vibration analyses for the isolated cascade and multi-blades cascade models have been conducted to show the physical fluid-structure interaction effects in the time domain.

• 정보저장시스템학회:학술대회논문집
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• 2005.10a
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• pp.248-253
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• 2005

The recording density of a hard disk drive is increasing so rapidly that the storage capacity of a commercial HDD in PC reaches several hundred giga bytes recently. Many technologies related to the HDD, such as servo, media, actuator dynamics, thermo and fluid dynamics, etc. must be developed together to realize high recording density. Especially, researches in the airflow inside the HDD cavity become important as the rotational speed of a disk increases. Typical problem due to the airflow is the vibration of an actuator as the airflow collides with an I-block, suspensions and sliders, that is, FIV(Flow Induced Vibration). This problem is one of the significant sources of increasing TMR so that it must be resolved. In this research, a disk damper shape has been modified to minimize the effects of airflow on the actuator. Modified disk dampers which change flow field inside HDD cavity show good effects not only on the disk (tufter but also on the vibration of an actuator. Vibrations of E- block and slider have been measured with LDV and the airflow field inside the HDD cavity has been analyzed using a commercial package to verify these effects.

• Nuclear Engineering and Technology
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• v.33 no.1
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• pp.73-82
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• 2001

Pressurized water reactor(PWR) fuel rods. which are continuously supported by a spring system called a spacer grid(SG), are exposed to reactor coolant at a flow velocity of up to 6-8 m/s. It is known that the vibration of 3 fuel rod is generated by the coolant flow, a so-called flow-induced-vibration(FIV), and the relative motion induced by the FIV between the fuel rod and the SG can wear away the surface of the fuel rod, which occasionally leads to its fretting failure. It is, therefore, important to understand the vibration characteristics of the fuel rod and reflect that in its design. In this paper, vibration analyses of the fuel rod with two different SGs were performed using both analytical and experimental methods. Updating of the finite element(FE) model using the measured data was performed in order to enhance confidence in the FE model of fuel rods supported by an SG. It was found that the modal parameters are very sensitive to the spring constant of the SG.

• Journal of the Korean Society for Aviation and Aeronautics
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• v.17 no.2
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• pp.8-17
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• 2009

In this study, flutter analyses for supercritical airfoil have been conducted in transonic region. Advanced computational analysis system based on computational fluid dynamics (CFD) and computational structural dynamics (CSD) has been developed in order to investigate detailed static and dynamic responses of supercritical airfoil. Reynolds-averaged Navier-Stokes equations with Spalart-Allmaras (S-A) and SST ${\kappa}-{\omega}$ turbulence models are solved for unsteady flow problems. A fully implicit time marching scheme based on the Newmark direct integration method is used for computing the coupled aeroelastic governing equations of cascades for fluid-structure interaction (FSI) problems. Also, flow-induced vibration (FIV) analyses for various supercritical airfoil models have been conducted. Detailed flutter responses for supercritical are presented to show the physical performance and vibration characteristics in various angle of attack.

• Transactions of the Society of Information Storage Systems
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• v.2 no.1
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• pp.32-37
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• 2006

The recording density of a hard disk drive(HDD) has been increased so rapidly that the storage capacity of a commercial HDD for the personal computer already reaches several hundred giga-bytes recently. Many technologies related to the HDD, such as servo, media, actuator dynamics, thermo and fluid dynamics, etc. must be developed together to realize higher recording density. Especially, airflow inside the HDD cavity has been concerned as the rotational speed of the disk increases. Typical problem due to the airflow is the off-track vibration of a head stack assembly(HSA) as the airflow collides with the E-block, suspensions, and sliders, i.e., the flow induced vibration(FIV). This problem is one of the most significant sources of the track mis-registration(TMR) so that it must be resolved. In this study, disk damper shape is modified to minimize the influence of airflow on the HSA. Modified disk dampers, which change the flow field of the inside cavity of a HDD, show good effects not only on the disk vibration but also on the off-track vibration of a HSA. Vibrations of E-block and slider have been measured with LDV and the airflow field inside the HDD cavity has been analyzed with commercial CFD program to verify these effects.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2002.11a
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• pp.389.2-389
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• 2002

Nonlinear aeroelastic characteristics of a missile control surface are investigated in this study. The wing model has freeplay structural nonlinearity at its pitch axis. Nonlinear aerodynamic flows with unsteady shock waves are also considered in high-speed flow region. To effectively consider a freeplay structural nonlinearity, the fictitious mass method (FMM) is applied to structural vibration analysis based on finite element method (FEM). (omitted)

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2005.05a
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• pp.312-317
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• 2005

Fluid-elastic instability and turbulence excitation for an under developing steam generator are investigated numerically. The stability ratio and the amplitude of turbulence excitation are obtained by using the PIAT (Program for Integrity Assessment of Steam Generator Tube) code from the information on the thermal-hydraulic data of the steam generator. The aspect ratio, the ratio between the height of U-tube from the upper most tube support plate (h) and the width of two vertical portion of U-tube (w), is defined for geometric parameter study. Several aspect ratios with relocation of tube support plates are adopted to study the effects on the mode shapes and characteristics of flow-induced vibration. When the aspect ratio exceeds value of 1, most of the mode shapes at low frequency are generated at the top of U-tube. It makes very high value of the stability ratio and the amplitude of turbulent excitation as well. We can consider that the local mode shape at the upper side of U-tube will develop the wear phenomena between the tube and the anti-vibration bars such as vertical, horizontal, and diagonal strips. It turns out that the aspect ratio reveals very important parameter for the design stage of the steam generator. The appropriate value of the aspect ratio should be specified and applied.

• Wind and Structures
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• v.23 no.2
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• pp.127-142
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• 2016

Aerodynamic effects, such as drag force and flow-induced vibration (FIV), on civil engineering structures can be minimized by optimally modifying the structure shape. This work investigates the turbulent wake of a square prism with its faces modified into a sinusoidal wave along the spanwise direction using three-dimensional large eddy simulation (LES) and particle image velocimetry (PIV) techniques at Reynolds number $Re_{Dm}$ = 16,500-22,000, based on the nominal width ($D_m$) of the prism and free-stream velocity ($U_{\infty}$). Two arrangements are considered: (i) the top and bottom faces of the prism are shaped into the sinusoidal waves (termed as WSP-A), and (ii) the front and rear faces are modified into the sinusoidal waves (WSP-B). The sinusoidal waves have a wavelength of $6D_m$ and an amplitude of $0.15D_m$. It has been found that the wavy faces lead to more three-dimensional free shear layers in the near wake than the flat faces (smooth square prism). As a result, the roll-up of shear layers is postponed. Furthermore, the near-wake vortical structures exhibit dominant periodic variations along the spanwise direction; the minimum (i.e., saddle) and maximum (i.e., node) cross-sections of the modified prisms have narrow and wide wakes, respectively. The wake recirculation bubble of the modified prism is wider and longer, compared with its smooth counterpart, thus resulting in a significant drag reduction and fluctuating lift suppression (up to 8.7% and 78.2%, respectively, for the case of WSP-A). Multiple dominant frequencies of vortex shedding, which are distinct from that of the smooth prism, are detected in the near wake of the wavy prisms. The present study may shed light on the understanding of the underlying physical mechanisms of FIV control, in terms of passive modification of the bluff-body shape.

• Journal of Veterinary Clinics
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• v.31 no.1
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• pp.57-60
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• 2014

A six-months-old male Korean domestic shorthair cat was presented with fever, tachypnea, anorexia, and weight loss and admitted to Lee Seung Jin Animal Medical Center. During the routine physical examination, clinical signs such as mild dehydration and jaundice in the sclera were present. The complete blood count (CBC) and serum chemistry result showed anemia, thrombocytopenia, neutrophilia, and hyperbilirubinemia. Radiography revealed hepatomegaly and splenomegaly. Blood smear and microscopic examination showed severe hemolysis and anisocytosis. We sent the blood sample to the Neodin Veterinary Laboratory for PCR analysis to conduct a test to find out Ehlichia, feline hemoplasmas (haemobartonella), feline leukemia virus (FeLV), feline immunodeficiency virus (FIV) and anaplasma infection. According to PCR examination, the blood of this cat was positive for feline hemoplasmas (Mycoplasma haemofelis), but negative for other pathogens. The patient was prescribed doxycycline for 4 weeks and prednisolone for 1 week. The free of feline hemoplasmas infection was confirmed by PCR recheck after six months.