• 대한의생명과학회지
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• 제25권1호
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• pp.75-82
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• 2019

We developed the multiplex LAMP assay using 16S rRNA, femA and mecA genes for direct detection of the methicillin resistance in Staphylococci from positive blood culture. To simultaneously recognize Staphylococci genus, S. aureus and methicillin resistance, three sets of six primers for 16S rRNA, femA and mecA were designed, respectively. The performance of LAMP assay was affirmed using VITEK system for the phenotypic methods of identification and for oxacillin and cefoxitin antimicrobial susceptibility. The optimal condition for LAMP assay was obtained under $64^{\circ}C$ for 50 min. The detection limit was determined to be of 20 copies and CFU/reaction ($10^4CFU/mL$). For clinical application of comparison with phenotypic methods, the sensitivity and specificity of the LAMP with femA gene for detecting S. aureus was 95.31% and 100%, respectively. The sensitivity and specificity of the LAMP with mecA gene for detecting methicillin resistance was 98.46% and 100%, respectively. The multiplex LAMP assay with femA and mecA gene successfully detected all of MRSA (38 isolates) isolates from 103 Staphylococci in blood cultures. The LAMP assay developed in this study is sensitive, specific, and of excellent agreement with the phenotypic methods.

• 화약ㆍ발파
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• 제41권3호
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• pp.13-25
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• 2023

종래의 벤치발파 공법은 한 발파의 모든 발파공에 역기폭을 적용하지만 MDS (mixture detonation system) 발파공법에서는 각 발파공의 공간적 또는 시간적 순서에 따라 정기폭과 역기폭을 교대로 적용한다. 공간적 순서에 따라 교대 적용하는 공법을 SMDS (spatial MDS), 시간적 순서에 따르는 공법을 TMDS (temporal MDS)라 부른다. 또 하나의 변종으로서 MMDS (modified MDS)가 있는데, 이 공법은 비산문제가 있는 현장을 위한 특수공법이다. 본 연구에서는 암석파괴 효과의 측면에서 MDS 발파공법을 종래공법과 비교한다. 비교는 LS-DYNA 상에서 2열 벤치발파 모델에 대한 수치적 시뮬레이션으로 이루어진다. 수치모델에서는 SPH-FEM 연계기법을 적용한다. SPH 요소는 발파공 부근의 근거리 영역의 암석에 적용하고, FEM 요소는 그 바깥의 원거리 영역에 적용한다. 암석에 대한 재료모델은 RHT 모델을 사용한다. 실제로 시뮬레이션을 수행한 결과, 벤치고 3.0 m, 저항선 1.6 m의 경우에 SMDS 발파공법이 종래공법에 비해 최대 0.4 m 더 깊은 파괴영역을 보였다. 덧붙여, MMDS 발파공법의 경우, 여타 공법들에 비해 벤치면 전방으로의 암편의 비산속도가 약 2.0 m/s 더 낮게 나타났다.

• Journal of Electrical Engineering and Technology
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• 제12권1호
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• pp.225-229
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• 2017

This paper describes the loss analysis based on load conditions of the air conditioning compressor motors using variable dc-link voltage. The losses of PMSM (Permanent Magnet Synchronous Motor) should be analyzed by the PWM (Pulse Width Modulation) output of inverter. The harmonic loss by the PWM cannot consider that using the current source analysis of the inverter. In addition, when the voltage of dc-link is variable with the condition of variable speed and load conditions in motor, the losses of motor are also changeable, however it is hard to analyze those losses by only electromagnetic finite element method (FEM). Therefore, this paper proposes the analysis method considering the carrier frequency of the inverter and the varying state of the dc-link voltage through the FEM-control coupled analysis. Using proposed analysis method, additional core loss and eddy current loss of permanent magnet caused by PWM could be analyzed. Finally, the validity of the proposed analysis method is verified through the comparison the result of coupled analysis with experiment.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2005년도 춘계학술대회논문집
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• pp.236-240
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• 2005

This paper deals with friction-induced vibration of disc brake system under constact friction coefficient. A linear, finite element model to represent the floating caliper disc brake system is proposed. The complex eigenvalues are used to investigate the dynamic stability and in order to verify simulations which are based on the FEM model, The comparison of experimental and analytical results shows a good agreement and the analysis indicates that mode coupling due to friction force and geometric instability is responsible fur disc brake squeal. And the Front brake system reduced the squeal noise using design of experiment method(DOE). This helped to validate the FEM model and establish confidence in the simulation results. Also they may be useful during real disk brake model.

• 한국안전학회지
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• 제17권2호
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• pp.8-15
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• 2002

In order to investigate the vibration characteristics of fluid-structure interaction problem, we modeled two identical rectangular plates coupled with fluid. A commercial computer code, ANSYS was used to perform finite element analysis and FEM solutions were compared with the experimental results to verify the finite element model. As a result, comparison of FEM and experiment showed good agreement, and the transverse vibration modes, in-phase and out-of-Phase, were observed alternately in the fluid-coupled system. The effect of fluid gap size on the fluid-coupled natural frequency were investigated. It was shown that the mode numbers increased, the normalized natural frequencies monotonically increased. And it was also found that an increase of the fluid gap reduced the coupled natural frequencies for the in-phase modes but increased the coupled natural frequencies for the out-of phase modes, and eventually converged to the results of an infinite fluid gap.

• 대한기계학회논문집A
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• 제26권12호
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• pp.2541-2549
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• 2002

In order to study the vibration characteristics of fluid-structure interaction problem, two rectangular plates coupled with bounded fluid were investigated. Experimental modal analyses were carried out to extract the modal parameters of the system. Additionally. finite element modal analyses performed using a commercial computer code, ANSYS. The FEM solutions were compared with the experimental solutions to verify the finite element model. As a result, the comparison between the experiment and FEM results showed excellent agreement. The transverse vibration modes, in-phase and out-of-phase, were observed alternately in the fluid-coupled system. The thickness effect of the plates on the fluid-coupled natural frequencies and mode shapes was investigated for two different cases with the identical thickness and the unequal thickness. It was found that the coupled natural frequencies increase with the thickness for the identical plates regardless of the mode phase. The experimental and the finite element analysis results showed that the out-of-phase mode shapes were deviated from the symmetrical mode shapes in the plate transverse direction fur the unequal plate thickness case.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2003년도 춘계학술대회
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• pp.839-844
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• 2003

The effect of water level on the free vibration of a partially water-filled two rectangular plates structure was investigated by experimental modal analysis and finite element analysis using ANSYS computer program. Modal parameters of two rectangular plates coupled with water were obtained by means of experiment and the FEM solutions were compared with the experimental solutions to verify the finite element model. As a result, the comparison between the experiment and FEM results showed excellent agreement. The transverse vibration modes, in-phase and out-of-phase, were observed alternately in the fluid-coupled system. The effect of water level and water gap size on the fluid-coupled natural frequency were investigated. It was found that the natural frequency of the partially water-filled two rectangular plates are not proportional to the water level, but depend on mode number of plates.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2001년도 춘계학술대회논문집A
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• pp.217-222
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• 2001

For the life evaluating of notched members, it is the best way that performing the real fatigue test of structure containing notch. But this method required generally much times and costs to evaluate fatigue life. So, generally we use the modified S-N curve or several methods to predict fatigue life. In this study, crack initiation life was evaluated by fatigue testing the SAE keyhole specimen and smooth specimen made of Al 7075-T6 alloys using the constant load then obtained S-N curve of smooth specimen and P-N curve of SAE keyhole specimen. And, fatigue lives of keyhole specimen are predicted using some life prediction methods (Nominal range I method, Nominal range II method, FEM analysis) for investigating experimented results, and that were compared with experimental data. Predicted fatigue lives by FEM analysis were corresponded with experimental data between 1/3times and 3times on the whole, and predicted fatigue lives using modified S-N curve (Nominal range I method, Nominal range II method) were nonconservative compared with that of FEM analysis.

• Journal of Advanced Marine Engineering and Technology
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• 제35권2호
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• pp.271-277
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• 2011

이 연구에서는 유전알고리즘을 이용하여 강봉의 감쇠행렬을 산출하는 방법을 제안하다. 감쇠행렬이 강성행렬과 비례한다는 가정을 전제로 각 요소강성행렬에 임의의 정수를 곱하여 감쇠행렬을 구성하여 주파수응답함수를 구성하고, 이를 실험 주파수응답함수와 비교한 값을 목적함수로 하여 목적함수가 가장 작은 정수의 감쇠행렬을 구한다. 비감쇠 해석의 경우보다 목적함수의 값이 약 1/60로 작아지는 것을 알 수 있었다. 이를 이용하면 큰 구조물의 감쇠가 큰 일부 부분구조물을 떼어내어 감쇠행렬을 구할 수 있어 구조물의 감쇠진동해석을 하는데 도움이 될 것으로 사료된다.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2002년도 춘계학술대회 논문집 유기절연재료 전자세라믹 방전플라즈마 일렉트렛트 및 응용기술
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• pp.132-137
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• 2002

The mold transformers have been widely used in underground substations in large building and have some advantages in comparison to oil-transformer, that is low fire risk, excellent environmental compatibility, compact size and high reliability. In addition, the application of mold transformer for outdoor is possible due to development of epoxy resin. The mold transformer generally has cooling duct between low voltage coil and high voltage coil. A mold transformer made by one body molding method has been developed for small size and low loss. The life of transformer is significantly dependent on the thermal behavior in windings. To analyse winding temperature rise, many transformer designer have calculated temperature distribution and hot spot point by finite element method(FEM). Recently, numerical analyses of transformer are studied for optimum design, that is electric field analysis, magnetic field, potential vibration, thermal distribution and thermal stress. In this paper, the temperature distribution of 50 kVA pole mold transformer for power distribution are investigated by FEM program and the temperature rise test of designed mold transformer carried out and test result is analyzed compare to simulation data. In this result, the designed mold transformer is satisfied to limit value of temperature and the other property is good such as voltage ratio, winding resistance, no-load loss, load loss, impedance voltage and percent regulation.