• The Journal of Pediatrics of Korean Medicine
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• v.10 no.1
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• pp.299-321
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• 1996

Experimental studies were done to research the effects of the Ursi fel and the UDCA on the anti-allergic effects. The results were obtained as follows: 1) In the experimental effects of the Ursi fel and the UDCA on the vascular permeability responses to intradermal Serotonin, though both of the Ursi fel and UDCA revealed the significant effect, the Ursi fel had stronger effect than the UDCA. 2) In the provocative effects of the Ursi fel and the UDCA on the vascular permeability responses to intradermal Histamin, though both of the Ursi powfel and UDCA showed the significant effect, the Ursi fel had moreerful effect than the UDCA. 3) In the 48 hours homologous passive cutaneous anaphylaxis provoked by the IgE-like antibody against white egg albumin, though both of the Ursi fel and UDCA revealed significant effect, the Ursi fel had stronger effect than the UDCA. 4) In the delayed type hypersensitivity response to Picryl Chlorede, though both of the Ursi fel and UDCA were proved to be effective significantly the Ursi fel showed stronger effect. 5) In the delayed type hypersensitivity responses to Sheep red blood cell, the Ursi fel revealed the significant effect, though the UDCA has no significant effect. According to above results, the Ursi fel was approved it could be used widely as antiallergic drug for immediate and delayed type allergic diseases. Although the UDCA revealed efficacy in immediated type allergic diseases, it had less powerful effects than the Ursi fel and it showed no effects in some experiment of delayed type allergy, so it would be difficult to be used clinically.

• Journal of Pharmacopuncture
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• v.11 no.3
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• pp.93-97
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• 2008

Objective: This study was done to investigate the safety of Fel Ursi pharmacopuncture solution manufactured with freezing dryness method to use eye drop. Methods: The eye irritation test of this material was performed according to the Regulation of Korea Food & Drug Administration(2005. 10. 21, KFDA 2005-60). After Fel Ursi pharmacopuncture solution was medicated in the left eye of the rabbits, the author observed eye irritation of the cornea, iris, conjunctiva at 1, 2, 3, 4 & 7day. Results: 1. After Fel Ursi pharmacopuncture solution was medicated in the left eye of the rabbits, there wasn't physical problem at 9 rabbits. 2. After Fel Ursi pharmacopuncture solution was medicated in the left eye of the rabbits, there wasn't eye irritation of the cornea, iris, conjunctiva at 1, 2, 3, 4 & 7day. Conclusions: I suggested that Fel Ursi pharmacopuncture solution didn't induced eye irritation in rabbits.

• Journal of Pharmacopuncture
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• v.11 no.3
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• pp.99-104
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• 2008

Objective: This study was done to investigate the safety of Fel Ursi & Bovis Calculus pharmacopuncture solution manufactured with freezing dryness method to use eye drop. Methods: The eye irritation test of this material was performed according to the Regulation of Korea Food & Drug Administration(2005. 10. 21, KFDA 2005-60). After Fel Ursi & Bovis Calculus pharmacopuncture solution was medicated in the left eye of the rabbits, the author observed eye irritation of the cornea, iris, conjunctiva at 1, 2, 3, 4 & 7day. Results: 1. After Fel Ursi & Bovis Calculus pharmacopuncture solution was medicated in the left eye of the rabbits, there wasn't physical problem at 9 rabbits. 2. After Fel Ursi & Bovis Calculus pharmacopuncture solution was medicated in the left eye of the rabbits, there wasn't eye irritation of the cornea, iris, conjunctiva at 1, 2, 3, 4 & 7day. Conclusions: I suggested that Fel Ursi & Bovis Calculus pharmacopuncture solution didn't induced eye irritation in rabbits.

• The Korea Journal of Herbology
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• v.20 no.2
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• pp.127-136
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• 2005

Objectives : The present study attempted to reveal the effects of the combination of Fel Ursi and Bezoar on the improvement of the function of the liver, through examining the effects of Fel Ursi, Bezoar, and Woong-Whang Tang composed of Fel Ursi and Bezoar. Method : Acute hepatitis was induced by galactosamine to rats, and then Fel Ursi, Bezoar Bovis, and Woong-Whang Tang were introjected to measure the influence of serums such as AST, ALT, ALP, ${\gamma}GT$, and lipid peroxide of liver tissues which are considered as the index of the function of the liver. Results : 1. Fel Ursi decreased the lipid peroxide of ALT, ${\gamma}FT$, and liver tissue and that of mitochondria in liver tissues of the rats with galactosamine-induced acute hepatitis, but it did not have any significant effect on AST and ALP. 2. Bezoar Bovis decreased the lipid peroxide of AST, ALT, ALP, ${\gamma}GT$, and the liver tissues among serum of the rats suffering from acute hapatitis induced by galactosamine, but it did not have any significant effect on that of mitochondria of the liver tissues. 3. Woong-Whang Tang had a significant effects on the lipid peroxide of AST, ALT, ALP, ${\gamma}GT$, and the liver tissues, and on the decrease of lipid peroxide of mitochondria, among serum of the rats suffering from acute hapatitis induced by galactosamine. Conclusion : Fel Ursi and Bezoar Bovis were judged to be effective on the acute hepatitis of the liver by galactosamine. In particular, Woong-Whang Tang which was composed of the combination of Fel Ursi and Bezoar Bovis was more efficient in the improvement of the function of the liver and the amount of lipid peroxide than the respective use of Fel Ursi or Bezoar Bovis.

• Journal of Pharmacopuncture
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• v.11 no.3
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• pp.105-111
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• 2008

Objective: The aim of this study is to investigate the safety of Fel Ursi, Bovis Calculus & Moschus pharmacopuncture solution manufactured with freezing dryness method to use eye drop. Methods: The eye irritation test of this material was performed according to the Regulation of Korea Food & Drug Administration(2005. 10. 21, KFDA 2005-60). After Fel Ursi, Bovis Calculus & Moschus pharmacopuncture solution was applied eyewash in the left eye of the rabbits, the author observed eye irritation of the cornea, iris, conjunctiva at 1, 2, 3, 4 & 7day. Results: 1. After Fel Ursi, Bovis Calculus & Moschus pharmacopuncture solution was applied eyewash in the left eye of the rabbits, there wasn t physical problem at 9 rabbits. 2. After Fel Ursi, Bovis Calculus & Moschus pharmacopuncture solution was applied eyewash in the left eye of the rabbits, there wasn't eye irritation of the cornea, iris, conjunctiva at 1, 2, 3, 4 & 7day. Conclusions: In this study, it was observed that Fel Ursi, Bovis Calculus & Moschus pharmacopuncture solution didn't induced eye irritation in rabbits.

• Journal of Pharmacopuncture
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• v.12 no.1
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• pp.13-20
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• 2009

Objective : This study developed species-specific PCR and PCR-RFLP to detect the adulteration of Fel ursi products with cattle and pig bile juices. Methods : All the primers for PCR and PCR-RFLP in this study were designed based on nucleotide sequences of cytochrome b genes in the mitochondria. Results : The species-specific PCR amplified a DNA fragment of 214, 214, 295, and 167 bp from Fel ursi product, bear fur, cattle bile juice, and pig bile juice, respectively. The survey using the speciesspecific PCR indicated that some of commercial Fel ursi products were adulterated with cattle and pig bile juices. PCR-RFLP using the restriction endonucleases, HaeIII and HinfI enabled differentiation among Fel ursi product, cattle bile juice, and pig bile juice. Bear furs from two animals showed variations in PCR-RFLP patterns with HaeIII. Discussion : The detection methods of the species-specific PCR and PCR-RFLP could be useful in eliminating adulterated Fel ursi products from the market.

• Journal of Acupuncture Research
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• v.24 no.4
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• pp.125-142
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• 2007

calculus pharmacopuncture. Porcine skin including fat tissue after treated Fel ursi and Bovis calculus pharmacopuncture by means of the dosage dependent variation are investigated the histologic changes after injection of these pharmacopuncture. Results: Following results were obtained from the preadipocyte proliferation and lipolysis of adipocyte and histologic investigation of fat tissue. 1. Fel ursi and Bovis calculus pharmacopuncture showed the effect of decreased preadipocyte proliferation on the high dosage($1mg/m{\ell}$). 2. Fel ursi pharmacopuncture showed the effect of decreased the activity of glycerol-3-phosphate dehydrogenase (GPDH) on the high dosage($1mg/m{\ell}$) and Bovis calculus pharmacopuncture significantly showed from $0.1mg/m{\ell}$ concentration. 3. Fel ursi pharmacopuncture was not showed the effect of lipolysis, but Bovis calculus pharmacopuncture was increased the effect of lipolysis in all concentration significantly. 4. Investigated the histological changes in porcine fat tissue after treated Fel ursi and Bovis calculus pharmacopuncture, we knew that these pharmacopuncture was showed significant activity to the lysis of cell membranes in all concentration. Conclusion : These results suggest that Fel ursi and Bovis calculus pharmacopuncture efficiently induces diminish proliferation of preadipocyte and lipolysis in adipose tissue.

• Journal of Pharmacopuncture
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• v.12 no.3
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• pp.41-47
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• 2009

Objectives : This experimental study was performed to investigate the efficacy of Fel Ursi, Bovis Calculus & Moschus pharmacopuncture solution manufactured with freezing dryness method to use eye drop. Methods : After administering Fel Ursi, Bovis Calculus & Moschus pharmacopuncture solution on bacterial species(Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, Candida albicans) which cause Keratitis, MIC(Minimum Inhibition Concentration) and the size of inhibition zone were measured. Anti-bacterial potency was also measured using the size of inhibition zone. Results : There was no response to MIC on bacterial species(Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, Candida albicans) after Fel Ursi, Bovis Calculus & Moschus pharmacopuncture solution was medicated. Conclusions : The present study suggests that Fel Ursi, Bovis Calculus & Moschus pharmacopuncture solution dosen't have anti-bacterial effects on bacterial species(Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, Candida albicans) which cause Keratitis. These study result recommends that more research on other herbal medicines of eye drop for Keratitis are required.

• Journal of Acupuncture Research
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• v.27 no.1
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• pp.65-74
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• 2010

Objectives : This study was to investigate the effects of Cervi Pantotrichum Cornu Pharmacopuncture and Bovis calculus Fel Ursi Pharmacopuncture on autonomic nervous system with Heart Rate Variability(HRV). Purpose of the trial was to observe what influence Cervi Pantotrichum Cornu Pharmacopuncture and Bovis calculus Fel Ursi pharmacopuncture have on the autonomic nervous system. Methods : 40 healthy male volunteers were divided into two experimental groups, such as Cervi Pantotrichum Cornu Pharmacopuncture group and Bovis calculus Fel Ursi Pharmacopuncture group. Study design was a randomized, double-blind clinical trial. 20 subjects in one experimental group were injected Cervi Pantotrichum Cornu Pharmacopuncture, 20 subjects in other experimental group were injected hydrolyzed Bovis calculus Fel Ursi Pharmacopuncture at GB21(Kyonjong). We measured HRV 7 times : baseline measurement and every 5 minutes for 30 minutes after injection. The SPSS 15.0 for Windows was used to analyze the data by the paired t-test(in group) and independent sample t-test(between the groups). Results A. Time Domain Analysis 1. Analysis of Mean HRV, SDNN and Complexity After injection of Cervi Pantotrichum Cornu Pharmacopuncture, Mean HRV was significantly decreased all the times after injection. SDNN was significantly increased at immediate and 25 minute after injection. Complexity was significantly decreased only at 15 minute after injection. After injection of Bovis calculus Fel Ursi Pharmacopuncture, Mean HRV was significantly decreased all the times after injection. Complexity was significantly decreased at 15 minutes after injection. 2. Analysis of HRV index, pNN50 After injection of Cervi Pantotrichum Cornu Pharmacopuncture, HRV index was significantly increased immediately after injection. pNN50 was significantly decreased at immediately, 20 and 25 minutes after injection. After injection of Bovis calculus Fel Ursi Pharmacopuncture, HRV index was significantly increased immediately after injection. pNN50 was significantly decreased at immediate and 25 minutes after injection. B. Frequency Domain Analysis 1. Analysis of Ln(TP), Ln(HF), Ln(LF), Ln(VLF) After injection of Cervi Pantotrichum Cornu Pharmacopuncture, Ln(TP) was significantly increased at immediate and 25 after injection. Ln(HF) was significantly increased all the times after injection. Ln(LF) was significantly increased at 15, 20 and 25 minutes after injection. Ln(VLF) was significantly increased at immediate and 25 minutes after injection. After injection of Bovis calculus Fel Ursi Pharmacopuncture, Ln(TP) was significantly increased at immediately and 25 after injection. Ln(LF) was significantly increased at 20 and 25 minutes after injection. Ln(VLF) was significantly increased at immediate and 25 minutes after injection. Conclusions : We results suggest that Cervi Pantotrichum Cornu Pharmacopuncture and Bovis calculus Fel Ursi Pharmacopuncture in healthy adult men tend to activate the autonomic nervous system and sympathetic nervous system within normal range.

• Herbal Formula Science
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• v.9 no.1
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• pp.231-250
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• 2001

In order to determine the effects of annexing bile extracts of bears on the anti-fibrotic effect of Injinhotang. Mix compound of Injinhotang and bile extracts of bears were administered to the carbon tetrachloride ($CCl_4$)-induced cirrhotic rats during 20 days and the changes of serum levels of GOT (glutamic-oxalacetic transaminase), GPT (glutamic pyruvic transaminase), LDH (lactate dehydrogenase), ALP (alanine phosphatase), GGT (gamma glutamyl transpeptidase) and T-BIL (total bilirubin) were monitored with comparison to the results of Injinhotang administered group. The results were summarized as follows. 1. A significant (p<0.01) increase of serum GOT levels were observed in control group compared to those of normal group but these increased levels were dramatically decreased in Injinhotang and Injinhotang with Fel Ursi-administered group. In addition, a significant (p<0.05) increase were also detected In Injinhotang with Fel Ursi-administered group compared to that of Injinhotang-administered group. 2. A significant (p<0.01) increase of serum GPT levels were observed in control group compared to those of normal group but these increased levels were dramatically decreased in Injinhotang and Injinhotang with Fel Ursi-administered group. Although significances were not recorded, increase of serum GPT levels were also detected in Injinhotang with Fel Ursi-administered group compared to that of Injinhotang-administered group. 3. A significant (p<0.01) increase of serum LDH levels were observed in control group compared to those of normal group but these increased levels were dramatically decreased in Injinhotang and Injinhotang with Fel Ursi-administered group. Although significances were not recorded, increase of serum LDH levels were also detected in Injinhotang with Fel Ursi-administered group compared to that of Injinhotang-administered group. 4. A significant (p<0.01 or p<0.05) increase of serum ALP levels were observed in control group compared to those of normal group but these increased levels were dramatically decreased in Injinhotang and Injinhotang with Fel Ursi-administered group. In addition, a significant (p<0.05) increase were also detected in Injinhotang with Fel Ursi-administered group compared to that of Injinhotang-administered group. 5. A significant (p<0.01) increase of serum GGT levels were observed in control and Injinhotang-administered group compared to those of normal group but these increased levels were dramatically decreased in Injinhotang with Fel Ursi-administered group. 6. A significant (p<0.01) increase of serum T-BIL levels were observed in control group compared to those of normal group but these increased levels were dramatically decreased in Injinhotang and Injinhotang with Fel Ursi-administered group. Although significances were not recorded, increase of serum T-BIL levels were also detected in Injinhotang with Fel Ursi-administered group compared to that of Injinhotang-administered group. In conclusion, it is considered that bile extract of bears has some additional effect to the anti-fibrotic effect of Injinhotang but to know the exact mechanism of suitable dose and duration of administration, further studies such as pharmacokinetics and dose-dependent pharmacological studies were needed