• Journal of Nutrition and Health
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• v.33 no.5
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• pp.491-496
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• 2000

Food intake is regulated by both central and peripheral mechanisms. In the central nervous, the hypothalamus acts for autonomic and endocrine homeostasis. The paraventricular nucleus(PVN) of hypothalamus is an imprtant site of interaction in central feeding pathways. Neuroepetide Y(NPY)is one of the most powerful neurochemical stimulants of food intake known. Also brain nitric oxide(NO), known as neurotransmitter, is involved in the mechanisms that regulate food intake. In this experiment, 24h fasting mice and anorexia mutant mice have been to examine the expression of NPY, which is the major neuropeptide increasing food intake. Double staining with NPY and nicotinamide-adenine-dinucleotide-phosphate diaphorase(NADPH-d), followed by immunohistochemical method and image analysis, have been used to observe coexisting neurons and the level of expression of each neurons. The results were as follows. 1) NPY-immunoreactivitys reduced immune response of the hypothalamus, particularly paraventricular nucleus(PVN), in anorexia mutant mice. Decreased level of NPY is assumed to be a major pathological factor in anorexia mutant mice. On the other hand, PVN in hypothalamus of fasting mice showed increased immunoreactivity which is in agreement of other researchers. 2) NPY and NADPH-d double staining revealed coexisting neurons in the cerebral cortex. Fasting mice had a tendency to have increased level of coexisting neurons compared to the control group. Compared to the control group, fasting mice express is not increase level of NPY-immunoreactivity, while anorexia mutant mice tended to have a decreased level.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.4 no.3
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• pp.95-103
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• 2023

This study aimed to identify the initial migratory macroinvertebrate species in two newly created mountain wetlands. To analyze the initial immigration species of macroinvertebrates, two experimental sites and two control sites were selected after habitat creation and investigated thrice from spring to fall. Benthic macroinvertebrates were collected quantitatively from each site using a Surber sampler and Modified D-frame deep net. After restoration at the two experimental sites, the number of macroinvertebrate species and individuals gradually increased from spring to fall and continued to increase over time with the development of waterside vegetation and habitat stabilization at the experimental sites. The species initially introduced to mountain wetlands after their creation were Ephemera strigata, Ephemera orientalis, Chironomidae sp., and Aquaris paludum. Subsequently, predators such as Davidius lunatus, Sieboldius albardae, Oyamia nigribasis, and Sialis longidens were introduced. Additionally, as a differentiating physicochemical factor between the two habitats, current velocity, which determines the distribution characteristics of benthic macroinvertebrates, and water temperature, which impacts the growth of organisms, showed a relatively stronger influence.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.21 no.4
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• pp.11-23
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• 2018

The purpose of this study is to identify the ecological characteristics and biological interactions between species of the abandoned rice paddy field in mountainous areas and to suggest a management strategy for stable food chain formation and biodiversity enhancement. The study site is located in Uldae wetland of Songchu district Bukhansan National Park, site characteristics and biological habitat characteristics were identified through site survey and literature survey. With regard to physical environment, among geographical features, the Uldae Wetland and the neighborhood inside the basin was a gently sloping area($5{\sim}15^{\circ}$). And 64.0% of basin faced the north. With regard to water environment, the Uldae Wetland was wetland of rainfed paddy field depending on precipitation and the system of stream flowing into the wetland from valley. According to the results of examining flora in plant ecology, in general, they were herbaceous wetland species. 88.6% of existing plants inside the Uldae Wetland basin was a forest in the mountain. And Quercus spp. community and Pinus densiflora community accounted for 64.6% of that, and was dominant. Except for that, Salix koreensis community was distributed. The existing vegetation of Uldae Wetland inhabited wetland species and terrestrialization indicator species, and it was thought that partial terrestrialization inside the Uldae Wetland was in progress after the discontinuation of paddy cultivation, such as the expansion of Salix koreensis distribution area. In the status of appearing faunae in the Uldae Wetland with regard to wildbirds of appearing principal species, The Uldae wetland was based on a abandoned rice paddy field various wildlife, and was a wildlife feeding, spawning, and resting place. The water environment was an important factor in maintaining the wetland living creatures function, habitat of waterbirds and benthic macroinvertebrates, amphibians and odonate are spawning ground and habitat, it was affecting the vegetation ecosystem based on wetlands. In order to maintain the diversity of wildlife, it was important to maintain smooth water supply and water level. A stable food chain will be formed and the Uldae wetland biodiversity will be abundant by establishing the relationship between the species of Uldae wetland, which is abandoned rice paddy field, and the habitat environment favored by species belonging to the ecosystem stepwise linkage. The ecological characteristics of the Uldae wetlands and the relation between the species were analyzed and the environmental conditions were reflected in the planning and management plan of Uldae wetland ecology.

• Korean Journal of Ecology and Environment
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• v.52 no.2
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• pp.105-117
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• 2019

In order to investigate the characteristics of benthic macroinvertebrate communities in the Gihwa stream, a tributary of the Dong River, we surveyed the community and environmental factors in April and November 2013 at 6 sites. The benthic macroinvertebrate taxa represented total 63 species belonging to 29 families, 12 orders, 5 classes and 4 phyla. Total 48 (10~28 in each site) species were collected in April and 44 (13~24 in each site) in November. The number of individuals increased slightly from $560{\sim}2,290m^{-2}$ in April to $806{\sim}3,674m^{-2}$ in November. Chironomidae spp. was dominant species in April and Stenopsyche bergeri was dominant species in November. In the Functional Feeding Groups, Gathering-collector(53.9%) was dominant in April, while Filtering-collector (44.3%) increased in November. Intolerant order category (i.e. EPT species richness) in St.1, St.5 and St.6 increased in November compared to April due to the increase of Trichoptera. St.2, St.3 and St.4, which were located near the fish farm, were low EPT as a whole, but Benthic macroinvertebrate index (BMI) was good state in November than April due to decrease of Chironomidae spp.. The environmental factors in the survey site showed similar tendency except for St.1 between both seasons, and electrical conductivity, salinity, and water width showed seasonal differences. Cluster analysis and Nonmetric multidimensional scaling (NMDS) based on benthic macroinvertebrate community data were divided into two groups according to season. Electrical conductivity, salinity and substrate composition were the most influential factors determining the distribution patterns of macroinvertebrate communities.

• Korean Journal of Environment and Ecology
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• v.27 no.3
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• pp.305-315
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• 2013

This study is aimed to provide the preliminary data for conservation and management of wintering site for Anser fabalis population in Cheongna Area, Incheon Free Economic Zone, Korea through analyzing the relationship between the biotop structure of its wintering site and behavior characteristics. The main types (size and ratio) of the biotope in Cheongna Area, Incheon Free Economic Zone are reed wetlands ($6,093,762m^2$, 47.8%), rice fields without rice straw ($2,927,916m^2$, 23.0%), and rice fields with rice straw ($1,915,655m^2$, 15.0%). According to the survey carried on 13th Feb., 2013, total 33 species and 6, 535 individual birds were observed and among of them, the dominant bird was Anser fabalis showing 5,128 indiviuals, 78% of total population. As the result of analyzing the migratory route of Anser fabalis, the bird moved from roosting site to foraging site before and after sunrise and from feeding site to roosting site before and after sunset. According to the analysis of interrelation between habitat characteristics and biotope types of the bird, population density was the highest in reed wetlands among habitat types and individual appearance was the highest in water-filled rice field melting ice. The bird ate the roots and bulb of hydrophytes in reed wetlands and showed various behaviors like eating dropped grains, resting and sleeping in water-filled rice fields and eating dropped grains and resting in rice fields with and without rice straw. It is shown that the number of Anser fabalis appearing in rice fields is depended on the presence of dropped grains than types of rice field.

• Korean Journal of Environment and Ecology
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• v.21 no.6
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• pp.526-535
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• 2007

This study was conducted at the Civilian Controlled Zone(CCZ) in the Cherowon from February to March 2004 to investigate the reaction to the vehicles of Red-crowned Cranes(RCC) and White-naped Cranes(WNC) and trade-off of the vehicle interruption with food resources. The for-aging distance from the road of WNC was much closer than that of RCC. The large flocks of cranes' average feeding distance from the road was farther than small flocks of cranes in both species. Cranes showed the reaction, such as alert, walking, running and flying against the vehicle stop and were more sensitive as they were close to the road. The reacting time to the stopping vehicle were reduced as it was farther from the road. The distance of about 250m was a reaction threshold distance against the vehicle stop to both species. The reacting time in the same distance was not different in relation to the traffic volume, but large traffic volume tended to cause cranes to stay far away from the road and not to feed themselves near a traffic congested area. The reason cranes fed themselves on fallen rice grains in spite of the vehicular traffic interruption factor was that there was a high density of the fallen rice grains remaining at the area near a road.

• Journal of Animal Science and Technology
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• v.62 no.5
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• pp.682-691
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• 2020

Careful cleaning and disinfection of pigpens is essential to prevent disease spread and avoid the resultant economic losses. Hygiene in pigpens is generally evaluated by visual monitoring supplemented with bacteriological monitoring, which includes counting the total aerobic bacteria (TAB) and/or fecal indicator bacteria (FIB). However, these methods present drawbacks such as time and labor requirements. As adenosine triphosphate (ATP) is ubiquitous in all living organisms including microorganisms, this study aimed to directly compare the results of microbial assessment and ATP quantification, and to suggest possible detailed application methods of the ATP test for hygiene evaluation in pigpens of a farrowing unit. Before and after standard cleaning procedures, samples were collected from the floor corner, floor center, and feeding trough of four pigpens at different time points. No FIB were detected and both the TAB and ATP levels were significantly decreased in the floor center area after cleaning. FIB were continuously detected after cleaning and disinfection of the floor corners, and there was no significant ATP level reduction. The feeding trough did not show any significant difference in these values before and after cleaning, indicating insufficient cleaning of this area. The levels of TAB and ATP after cleaning were significantly correlated and the average ATP value was significantly lower in the absence of FIB than in their presence. In the absence of standard references, a more thorough hygiene management could be achieved evenly by supplementing cleaning or disinfection based on the lowest ATP results obtained at the cleanest test site, which in the present study was the floor center. Overall, these results indicate that the on-farm ATP test can be used to determine the cleanliness status, in addition to visual inspection, as an alternative to laboratory culture-based testing for the presence of microorganisms.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.22 no.4
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• pp.430-435
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• 2000

Cleft lip and palate is one of the congenital anomalies which need comprehensive and multidisciplinary treatment plan because 1) oral cavity is an important organ with masticatory function as a start of digestive tract, 2) anatomic symmetry and balance is esthetically important in midfacial area, and 3) it is also important to prevent psycho-social problems by adequate restoration of normal facial appearance. There are many different protocols in the treatment of cleft lip and palate, but our department has adopted and modified the $Z{\"{u}}rich$ protocol, as published in the Journal of Korean Cleft Lip and Palate Association in 1998. The first challenge is feeding. Type of feeding aid ranges from simple obturators to active orthopedic appliances. In our department we use passive-type plate made up of soft and hard acrylic resin which permits normal maxillary growth. We use Millard's method to restore normal appearance and function of unilateral complete cleft lip. In consideration of both maxillary growth and phonetic problems, we first close soft palate at 18 months of age and delay the hard palate palatoplasty until 4 to 5 years of age. When soft palate is closed, posterior third of the hard palate is intentionally not denuded to allow normal maxillary growth. In hard palate palatoplasty the mucoperiosteum of affected site is not mobilized to permit residual growth of the maxilla. We have treated a patient with unilateral complete cleft lip and palate by Ajou protocol, which is a kind of modified $Z{\"{u}}rich$ protocol. It is as follows: Infantile orthopedics with passive-type plate such as Hotz plate, cheiloplasty with Millard's rotation-advancement flap, and two stage palatoplasty. It is followed by orthodontic treatment and secondary osteoplasty to augment cleft alveolus, orthognathic surgery, and finally rehabilitation with conventional prosthodontic treatment or implant installation. The result was good up to now, but we are later to investigate the final result with longitudinal follow-up study according to master plan by Ajou protocol.

• Journal of Ecology and Environment
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• v.41 no.3
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• pp.45-53
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• 2017

Background: There have been many studies on the growth conditions of Zostera marina and Zostera japonica, but few studies have examined how spatial and temporal factors affect growth in established seagrass beds or the distribution range and shoot density. This study aims to clarify the factors that determine the temporal and spatial distribution of Zostera marina and Zostera japonica in the Seto Inland Sea east of Yamaguchi Prefecture. Methods: The study site is in Hiroshima Bay of the Seto Inland Sea, along the east coast of Yamaguchi Prefecture, Japan. We monitored by diving observation to confirm shoot density, presence or absence of both species and observed water temperature, salinity by sensor in study sites. Results: The frequency of occurrence of Zostera marina was high in all seasons, even in water depths of D.L. + 1 to -5 m ($80{\pm}34%$ to $89{\pm}19%$; mean ${\pm}$ standard deviation), but lower (as low as $43{\pm}34%$) near the breakwall, where datum level was 1 to 2 m, and it was further reduced in datum level -5 m and deeper. The frequency of occurrence of Zostera japonica was highest in water with a datum level of +1 to 0 m. However, in datum level of 0 m or deeper, it became lower as the water depth became deeper. Datum level +1 m to 0 m was an optimal water depth for both species. The frequency of occurrence and the shoot density of both species showed no negative correlation. In 2011, the daily mean water temperature was $10^{\circ}C$ or less on more days than in other years and the feeding damage by S. fuscescens in the study sites caused damage at the tips. Conclusions: We considered that the relationship between these species at the optimal water depth was not competitive, but due to differences in spatial distribution, Zostera marina and Zostera japonica do not influence each other due to temperature conditions and feeding damage and other environmental conditions. Zostera japonica required light intensity than Zostera marina, and the water depth played an important role in the distribution of both species.

• Journal of Nutrition and Health
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• v.37 no.7
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• pp.540-549
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• 2004

Infants and children with food related Atopic Dermatitis (AD) need extra dietary efforts to maintain optimal nutrition due to food restriction to prevent allergy reactions. However, nutrition ignorance and food faddism make patients even more confused and practice desirable diet more difficult. The objective of this study was to report the AD patients' malnutrition cases in Korea. We report on 2 cases of severe nutritional deficiency caused by consuming macrobiotic diets which avoid processed foods and most animal foods, i.e. one of vegetarian diet. Case 1, a 12-month-old male child, was admitted with severe marasmus. Because of a history of AD, he was started on mixed grain porridge at 3 months without any breast milk or formula feeding. His caloric intake was 66% and protein intake was 69% of the recommended dietary allowance. Patient's height and weight was under 3th percentile. On admission the patient was unable to crawl or roll over. Case 2, a 9-month-old AD female patient, was diagnosed with kwashiorkor and rickets. She was also started on mixed grain porridge at 100 days due to AD. Her caloric intake has been satisfied recommended dietary allowance until 7 months, however, she conducted sauna bath therapy and reduced both energy and protein intake at 8 months. The amount of protein intake for case 2 was higher than recommended dietary allowance, but, sauna therapy and severe AD with intakes of low guality protein may increase patient's protein requirement resulting in kwashiorkor. Case 2 patient's height and weight was on 3th percentile. Both cases showed low intake of calcium, iron, zinc, vitamin A, vitamin E and especially very low intake of vitamin B$_{12}$ and vitamin D. Allergy tests for certain foods had not done prior to admission for both cases. They followed the dietary advise operated by macrobiotic diet internet site. In conclusion, AD infants' parents and caregivers should contact a pediatrician trained as a specialist in allergy for accurate diagnosis. For infant patients, breast or formula feeding including hypoallergenic formula should be continued until their one year of age. When certain foods need to be restricted or to follow special diets such as vegetarian diet, consultation with pediatrician and dietitian is needed.d.