• Title/Summary/Keyword: FC

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Differential regulation of phospholipase $C\gamma$ isoforms through Fc$\varepsilon$RI, high affinity IgE receptor

  • Yoon, Eung-Joo;Beom, Sun-Ryeo;Kim, Kyeong-Man
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.93.3-94
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    • 2003
  • The signaling components of high affinity IgE receptor (Fc RI) were searched by yeast-hybrid screening of the cDNA library constructed from RBL-2H3 cells. The cytoplasmic part of the Fc RI- chain was found to specifically interact with PLC 2, and further comparatives studies were conducted focusing on the differential regulation of two PLC- isoforms through Fc RI. The inhibitors of Src, Syk, and protein kinase C similarly affected the tyrosine phosporylations of PLC 1 and PLC 2 but the inhibitors of PI3-kinase and p42/44 ERK effectively inhibited the activation of PLC 1 but not PLC 2. (omitted)

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A Study on the actual conditions of college students′ fast-food use and their likings -Centering around the students in the Uijungbu region- (대학생들의 패스트푸드 이용 실태와 기호도에 관한 연구 -의정부 지역 대학생 중심으로-)

  • 이명호;김동섭
    • Culinary science and hospitality research
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    • v.6 no.3
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    • pp.329-342
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    • 2000
  • The demographic characteristics of research examinees showed that male students were 122(44%) and female ones 155(56%), and teenagers 142 per age. The average monthly pocket money appeared that 96 students received more than 200,000(34.7%) and it was analyzed that 243(87.7%) resided in their homes. The research of fast-food choice factors resulted in the fact that female students who were easily influenced by their friends were 83, while males 57. And, mascom took influence on 40 female students and 20 male ones, besides other included 39 male and 30 female students. They most preferred hamburger and pizza(94) among the fast-food menus, and taste like seasame was most preferred among the characteristic factors of foods taste. And as a result of factor analysis of menu, Cronbach alpha coefficient Fc 2 of reliability appeared highest as 0.7694, next, Fc 3 as 0.6965, and Fc 1 lowest as 0.6403. The reliability among the specific items of this study may be regarded high. And the difference between groups according to gender appeared that Fc 1, Fc 2, and Fc 3 were significantly different at 5% level as a result of T-examination analysis and the distributed analysis of the average monthly pocket money resulted in the fact that the difference between groups appeared significantly at F3 below significant level of 0.05. The testing of the difference of reason why the fast-food is preferred according to frequency of use per week lead to Chi-square value of 41.399 with free degree of 12, so significant degree became 0.000. The zero hypothesis that the two variables are not independent each other is not reserved because it has a small value of signigicant degree below 0.05, and the permanent hypothesis is adopted.

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Effects of Natural Products on the Induction of NAD(P)H: Quinone Reductase in Hepa 1c1c7 Cells for the Development of Cancer Chemopreventive Agents

  • Kim, Young-Mi;Chang, Il-Moo;Mar, Woong-Chon
    • Natural Product Sciences
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    • v.3 no.2
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    • pp.81-88
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    • 1997
  • NAD(P)H:quinone reductase (QR) is one of the protective phase II enzymes against toxicity that accomplishes the capacity of detoxification by modulating the effects of mutagens and carcinogens. The detoxification mechanism is that quinone reductase promotes the 2-electron reduction of quinones to hydroquinones which are less reactive. This study is to search new inducers of quinone reductase from natural products, which can be used as cancer chemopreventive agents. Plant extracts were evaluated by using quinone reductase generating system With Hepa 1c1c7 murine hepatoma cell lines for enzyme inducing properties and crystal violet staining method for the measurement of cytotoxicity provoked. We have tested approximately 106 kinds of natural products after partition into n-hexane, ethyl acetate and aqueous layers from 100% methanol extracts of natural products. The ethyl acetate fractions of Vitex rotundifolia $(fruits,\;2FC:\;12.7\;{\mu}g/ml)$, Cnidium officinale $(aerial\;parts,\;2FC:\;10.5\;{\mu}g/ml)$, Chrysanthemum sinese $(flowers,\;2FC:\;17.4{\mu}g/ml)$ and the hexane fractions of Angelica gigas $(roots,\;2FC:\;13.2\;{\mu}g/ml)$, Smilax china $(roots,\;2FC:\;l1.9\;{\mu}g/ml)$, Sophora flavescens $(roots,\;2FC:\;16.3\;{\mu}g/ml)$ revealed the significant induction of quinone reductase in a murine hepatic Hepa 1c1c7 cell culture system.

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Effects of different physical forms of concentrate on performance, carcass characteristics, and economic analysis in hanwoo steers

  • Kim, Sung Il;Seo, Bo Cheon;Jang, In Surk;Kim, Ouk;Choi, Chang Bon;Jung, Keun Ki
    • Journal of Animal Science and Technology
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    • v.56 no.3
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    • pp.9.1-9.8
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    • 2014
  • This study was performed to investigate the effects of different forms of concentrate fed to Hanwoo steers on performance, carcass characteristics, and economic performance. Forty-two Hanwoo steers (average age of $5.1{\pm}0.8mo$. with body weight of $147.05{\pm}10.85kg$) were randomly allotted into FC (animals fed flakes for entire experimental period) and GC (animals fed grounded concentrate during growing and fattening phases followed by flaked concentrate during finishing phase) groups for 758 d after reaching an age of $30.0{\pm}0.82mo$. There was no difference in body weight (BW) or ADG between the treatments until fattening (15 ~ 22 mo.) phase. However, by finishing phase (23 ~ 30 mo.), the GC group (739.24 kg BW and 0.67 kg ADG) showed greater (P < 0.05) BW and ADG than the FC group (702.93 kg BW and 0.59 kg ADG). Steers in the GC group also showed greater (P < 0.05) BW and ADG than the FC group throughout the entire experimental period (5 ~ 30 mo.). There was no significant difference in carcass weight or backfat thickness between the treatments. M. Longissimus dorsi area of the GC group ($91.00cm^2$) was greater (P < 0.05) than that of the FC group ($83.59cm^2$). Marbling score and percentage of $1^{{+}{+}}$ meat quality grade were 14.0 and 48.0% higher in the GC group compared to the FC group. There was no significant difference in physicochemical characteristics, including moisture and crude protein levels, between the treatments. Gross income per head excluding operating expenses was 59.3% greater in the GC group (1,647,512 won) compared to the FC group (1,034,343 won).

Classification and Genetic Variation Analysis Among Formae Speciales of Fusarium oxysporum by Using Recombinant DNA Probes (재조합 DNA probe에 의한 Fusarium oxysporum 분화형간의 분류 및 유전적 변이 분석)

  • Kim, Young-Tae;Kim, Hong-Gi
    • The Korean Journal of Mycology
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    • v.25 no.4 s.83
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    • pp.362-368
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    • 1997
  • Five formae speciales of Fusarium oxysporum in Korea were examined using RFLP analysis to find the possibility for classification and analyze genetic variations. DNAs from F. oxysporum f. sp. lycopersici, cucumerinum, fragariae, garlic and sesami were used with three recombinant probes such as pFC46, pFC52 and pFC57. Distinct differences among five formae speciales of this fungus were detected in RFLP band patterns based on southern hybridization of genomic DNA using each recombinant clone, which was a repetitive copy probe. Strains belong to four formae speciales could be very stable in genetic variation except f. sp. sesami which has more variation than the others based on the RFLP analysis. They formed their own cluster which has high similarity within the same formae specialis resulted from the UPGMA analysis for genetic relationship analysis and each cluster represented its own formae specialis. The method using three recombinant DNA probes could be a good tool for classification of formae speciales in F. oxysporum.

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Vaccinium angustifolium Root Extract Suppresses FcεRI Expression in Human Basophilic KU812F Cells.

  • Shim, Sun Yup;Lee, Kyung Dong;Lee, Mina
    • Preventive Nutrition and Food Science
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    • v.22 no.1
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    • pp.9-15
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    • 2017
  • Vaccinium angustifolium, commonly known as the lowbush blueberry, is a rich source of flavonoids, with which various human physiological activities have been associated. The present study focuses on the investigation of the effect of the methanolic extract of V. angustifolium root extract (VAE) on high affinity immunoglobulin E receptor ($Fc{\varepsilon}RI$) ${\alpha}$ chain antibody (CRA-1)-induced allergic reaction in human basophilic KU812F cells. The total phenolic content of VAE was found to be $170{\pm}1.9mg$ gallic acid equivalents/g. Flow cytometry analysis revealed that the cell surface expression of $Fc{\varepsilon}RI$ was suppressed in a concentration-dependent manner upon culture with VAE. Reverse-transcriptase polymerase chain reaction analysis showed that the mRNA level of the $Fc{\varepsilon}RI$ ${\alpha}$ chain was reduced in a concentration-dependent manner as a result of VAE treatment. Western blot analysis revealed that the protein expression of $Fc{\varepsilon}RI$ and the phosphorylation of extracellular signal-regulated kinases (ERK) 1/2 were concentration-dependently inhibited by VAE. We determined that VAE inhibited anti-CRA-1-induced histamine release, in addition to the elevation of intracellular calcium concentration ([$Ca^{2+}$]i), in a concentration-dependent manner. These results indicate that VAE may exert an anti-allergic effect via the inhibition of calcium influx and histamine release, which occurs as a result of the down-regulation of $Fc{\varepsilon}RI$ expression through inhibition of ERK 1/2 activation.

Research about VVVF direct subways Secondary source unit(SIV)180KVA for using Filter Capacitor(FC) maintenance & limit of use (VVVF 직류전동차의 보조전원장치(SIV)180KVA용에 사용되는 필터콘덴서(FC) 유지보수 및 사용한도에 관한 연구)

  • Shin, Hye-Jin;Woo, Suk-Tae;Shin, Min-Ho;Son, Young-Jin
    • Proceedings of the KSR Conference
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    • 2010.06a
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    • pp.1345-1351
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    • 2010
  • First, In this essay we research about secondary source unit(SIV)180KVA is install on VVVF direct subways for using Filter Capacitor(FC) maintenance & limit of use. Vehicle's extendly use and congestly at load for Catenary voltage and load change about output change and in SIV unit there are important device named GTO Thyristor, Power Transistor Module device become short and it often happen. We analysis the fault cause and there are effects and we know the secondary source unit in filter capacitor FC1~4 input harmonic distortion component's filtering don't do correctly so we study the capacitor extend use for the component change and the outside condition by slow property changed happen and we check before and when its fault, its difficult to measure. Finally, In this research we care for this reason and we study about capacitor maintenance through control and measure ways improvement and capacitor's life spans limit of use.

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