The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Classification and Genetic Variation Analysis Among Formae Speciales of Fusarium oxysporum by Using Recombinant DNA Probes

재조합 DNA probe에 의한 Fusarium oxysporum 분화형간의 분류 및 유전적 변이 분석

  • Kim, Young-Tae (Department of Agricultural Biology, Chungnam National University) ;
  • Kim, Hong-Gi (Department of Agricultural Biology, Chungnam National University)
  • 김영태 (충남대학교 농과대학 농생물학과) ;
  • 김홍기 (충남대학교 농과대학 농생물학과)
  • Published : 1997.12.30
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Abstract

Five formae speciales of Fusarium oxysporum in Korea were examined using RFLP analysis to find the possibility for classification and analyze genetic variations. DNAs from F. oxysporum f. sp. lycopersici, cucumerinum, fragariae, garlic and sesami were used with three recombinant probes such as pFC46, pFC52 and pFC57. Distinct differences among five formae speciales of this fungus were detected in RFLP band patterns based on southern hybridization of genomic DNA using each recombinant clone, which was a repetitive copy probe. Strains belong to four formae speciales could be very stable in genetic variation except f. sp. sesami which has more variation than the others based on the RFLP analysis. They formed their own cluster which has high similarity within the same formae specialis resulted from the UPGMA analysis for genetic relationship analysis and each cluster represented its own formae specialis. The method using three recombinant DNA probes could be a good tool for classification of formae speciales in F. oxysporum.

재조합 DNA probe를 이용하여 국내 Fusarium oxysporum 분화형간의 분류 가능성을 탐색하고 그들의 유전적 변이를 분석하였다. F. oxysporum f. sp. lycopersici, cucumerinum, fragariae, garlic, sesami 등 5종의 분화형을 공시하여 RFLP 분석을 실시하였다. Repetitive copy clone인 세종의 재조합 클론 pFC46, pFC52, pFC57을 이용하여 HindIII로 처리한 F. oxysporum의 genomic DNA에 대해 southern hybridization한 결과 나타난 밴드의 양상은 분화형에 따라 차이가 명확히 밝혀져 이들을 이용한 분류가 가능하였다. 또한 RFLP 분석 결과 f. sp. sesami는 다른 분화형에 비해 다소 변이가 심했으나 다른 분화형들은 변이가 거의 없어 f. sp. sesami를 제외한 f. sp. lycopersici 등 4종의 Fusarium oxysporum 분화형의 균주들은 채집 지역에 관계없이 대체로 유전적으로 안정되어 있는 것으로 판단되었다. Hybridization밴드의 양상에 기초하여 유전적 유연관계를 집괴 분석한 결과 각 분화형별로 유사도가 매우 높게 별도의 유사군을 형성하였다.

Keywords

References

  1. Exp. Mycol. v.19 Intraspecific variation within population of Fusarium oxysporum based on RFLP analysis of the intergenic spacer region of the rDNA Appel, D.;Gordon, T.R.
  2. Can J. Bot. v.65 An evaluation of F. oxysporum from crucifers based on pathogenicity, isozyme polymorphism, vegetative compatibility and geographic origin Bosland, P.W.;Williams, P.H.
  3. Phytopathology v.77 Nitrate nonutilizing mutants of Fusarium oxysporum and their use in vegetative compatibility tests Correll, J.C.;Klittich, C.J.R.;Leslie, J.F.
  4. Phytopathology v.85 Comparison of three molecular methods for the characterization of Fusarium oxysporum strains Edel, V.;Steinberg, C.;Avelange, I.;Laguerre, G.;Alabouvette, C.
  5. Mol. Plant-Microbe Inter. v.6 Population structure of Fusarium oxysporum f. sp. lycopersici: Restriction fragment length polymorphisms provide genetic evidence that vegetative compatibility group is an indicator of evolutionary origin Elias, K.S.;Zamir, D.;Lichtman-Pleban, T.;Katan, T.
  6. Phytopathology v.41 The pathogenecity of race 1 and 2 of Fusarium oxysporum f. sp. lycopersici Gerdemann, J.W.;Finley, A.M.
  7. Plant Dis. v.66 Occurrence of a third race of Fusarium wilt of tomatoes in Queensland Grattige, R.;O'Brien, R.G.
  8. Kor. J. Plant Pathol. v.10 Potential application of recombinant DNA probes for relatedness analysis of Fusarium oxysporum Kim, H.G.;Kim, Y.T.;Yu, S.H.
  9. Phytopathology v.77 Relatedness of strains of Fusarium oxysporum from Crucifers measured by examination of mitochondrial and ribosomal DNA Kistler, H.C.;Bosland, P.W.;Benny, U.
  10. Phytopathology v.81 Repetitive genomic sequence for determining relatedness among strains of Fusarium oxysporum Kistler, H.C.;Momol, E.A.;Benny, U.
  11. Trans. Mycol. Soc. Japan v.33 Genetic diversity of Fusarium oxysporum f. sp. lycopersici in restriction fragment length polymorphisms of mitochondrial DNA Kuninaga, S.;Yokosawa, R.
  12. Phytopathology v.77 Potential application of random DNA probes and restriction fragment length polymorphisms in the taxonomy of the Fusaria Manicom, B.Q.;Bar-Joseph, M.;Rosner, A.;Vigodsky-Haas, H.;Kortze, J.M.
  13. Phytopathology v.80 A restriction fragment length polymorphism probe relating vegetative compatibility groups and pathogenicity in Fusarium oxysporum f. sp. dianthi Manicom, B.Q.;Bar-Joseph, M.;Kortze, J.M.;Bekcer M.M.
  14. Can. J. Bot. v.63 Classification of strains of Fusarium oxysporum on the basis of vegetative compatibility Puhalla, J.E.
  15. NTSYS-pc. Numerical taxonomy and multivariate analysis system Rohlf, F.J.
  16. Molecular Cloning: A Laboratory manual Sambrook, J.;Fritsch, E.F.;Maniatis, T.