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Evaluation of genotype by environment interactions on milk production traits of Holstein cows in southern Brazil

  • Moreira, Raphael Patrick;Pinto, Luis Fernando Batista;Valloto, Altair Antonio;Pedrosa, Victor Breno
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.4
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    • pp.459-466
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    • 2019
  • Objective: This study assessed the possible existence of genotype by environment interactions for milk, fat and protein yields in Holstein cattle raised in one of the most important milk production basins in Brazil. Methods: Changes in the genetic parameters and breeding values were evaluated for 57,967 animals from three distinct regions of southern Brazil, divided according to differences in climate. The genotype by environment interaction was determined by genetic correlations between regions, estimated by the restricted maximum likelihood, considering the animal model. Bull rankings were investigated to verify the ratio of coincident selected animals between regions for each trait. Results: The estimates of heritability coefficients were similar between two regions, but were lower in the third evaluated area, for all traits. Genetic correlations between regions were high, ranging from 0.91 to 0.99 for milk, fat and protein yields, representing the absence of a genotype by environment interaction for productive traits. The percentage of selection error between regions for the top 10% of animals ranged from 0.88% to 2.07% for milk yield, 0.99% to 2.46% for fat yield and 0.59% to 3.15% for protein yield. Conclusion: A slight change in genotype between areas was expected since no significant genotype by environment interactions were identified, facilitating the process of selecting Holstein cattle in southern Brazil.

Dietary Intake of High School Students in the Dormitories (전북지역 남여 고등학교 기숙사 학생들의 영양섭취 실태 조사)

  • 한숙경;권태봉
    • The Korean Journal of Food And Nutrition
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    • v.6 no.2
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    • pp.115-120
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    • 1993
  • A nutrition survey of 80 high school students in urban dormitories, 17-19 year old, in Jeonbuk province was undertaken in August of 1990, to investigate dietary intake. Total energy intake of male and female was 2,246.0 and 1,584.9 kcal respectively. Energy Intakes from breakfast, lunch and dinner were 553.5, 683.4 and 1,023.0 kcal in male, and 372.9, 391.8 and 820.2 local in female. Protein daily intake of male and female was 95.3 and 62.9g, and carbohydrate intake was 368.1g and 259.6g respectively. Total energy intake of male student was composed of carbohydrate 65%. protein 16.8% and fat 18.2% and that of female was carbohydrate 65.5%, protein 15.9% and fat 18.6% respectively.

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Expression of Adipocyte-Associated Genes in the Mouse Tail Epididymal Fat at Different Postnatal Ages

  • Lee, Ki-Ho
    • Development and Reproduction
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    • v.24 no.3
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    • pp.167-176
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    • 2020
  • The fat pad defined as the epididymal fat is located at the head part of the epididymis. However, another fat mass is present near the caudal epididymis, named tail epididymal fat. The present research was focused to determine the expression of adipocyte-associated molecules in the mouse tail epididymal fat at different postnatal ages, including 2, 5, 8, and 12 months of age. The quantitative real-time PCR analysis showed continuous increases of expression levels of delta like non-canonical Notch ligand 1, leptin, and resistin as postnatally aged. The transcript level of peroxisome proliferator-activated receptor gamma was the highest at 5 months of age, remaining at a steady level until 12 months of age. Expression levels of fatty acid binding protein 4, leptin, and adiponectin were significantly increased until 8 months of age, persisting the level at 12 months of age. The transcript level of fatty acid synthase was significantly increased at 8 months of age, without a further change of the level at 12 months of age. These findings exhibited the expression of adipocyte-associated genes which were also detected at the ordinary epididymal fat pad. However, expression patterns of these genes in the tail epididymal fat are different with those in the distal and proximal epididymal fat, suggesting distinct characteristics and/or functions of the tail epididymal fat.

Effect of Folic Acid on Economic Traits and the Change of Some Metabolic Substances of the Silkworm, Bombyx mori L.

  • Nirwani, R.B.;Kaliwal, B.B.
    • Journal of Sericultural and Entomological Science
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    • v.38 no.2
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    • pp.118-123
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    • 1996
  • Dietary supplementation of folic acid to silkworm larvae in different concentration (100, 200 and 300 ${\mu}$g/ml) to the 4th and the 5th instar resulted in a significant increase in economic parameters, such as female and male cocoon weights, shell weights, egg productivity and egg hatching percentage. However, larval duration was significantly decreased, as compared with that of carrier control. The increase in the glycogen and protein contents of the fat body was not significant, whereas the increase in trehalose content of haemolymph in all the treated groups and protein content of fat body and haemolymph in 100 ${\mu}$g/ml treated groups were significant. However, the haemolymph protein was significantly decreased in 300${\mu}$g/ml treated group.

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Research on Real-Time Portable Quality Evaluation System for Raw Milk

  • Lee, Dae Hyun;Kim, Yong Joo;Min, Kyu Ho;Choi, Chang Hyun
    • Agribusiness and Information Management
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    • v.6 no.2
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    • pp.32-39
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    • 2014
  • The goal of this research was to develop a portable system that could be used to evaluate the quality of milk in real time at a raw milk production site. A real-time portable quality evaluation system for raw milk was developed to enable non-destructive quality evaluation of somatic cell count (SCC), fat, protein, lactose, and total solid (TS) in milk samples. A prediction model of SCC, fat, protein, lactose, and TS was constructed using partial least squares (PLS) and 200 milk samples were used to evaluate the prediction performance of the portable quality evaluation system and high performance spectroscopy. Through prediction model development and verification, it was found that the accuracy of high performance spectroscopy was 90% for SSC, 96% for fat, 96% for protein, 91% for lactose, and 97% for TS. In comparison, the accuracy of the portable quality evaluation system was relatively low, at 90% for SSC, 95% for fat, 92% for protein, 89% for lactose, 92% for TS. However, the measurement time for high performance spectroscopy was 10 minutes for 1 sample, while for the portable quality evaluation system it was 6 minutes. This means that the high performance spectroscopy system can measure 48 samples per day (8 hours), while the portable quality evaluation system can measure 80 (8 hours). Therefore, it was found that the portable quality evaluation system enables quick on-site quality evaluation of milk samples.

Genetic parameter estimation for milk β-hydroxybutyrate and acetone in early lactation and its association with fat to protein ratio and energy balance in Korean Holstein cattle

  • Ranaraja, Umanthi;Cho, KwangHyun;Park, MiNa;Kim, SiDong;Lee, SeokHyun;Do, ChangHee
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.6
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    • pp.798-803
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    • 2018
  • Objective: The objective of this study was to estimate the genetic parameters for milk ${\beta}$-hydroxybutyrate (BHBA), acetone (Ac), fat protein ratio (FPR), and energy balance (EB) using milk test day records and investigate the effect of early lactation FPR and EB on milk ketone body concentrations. Methods: Total 262,940 test-day records collected from Korea Animal Improvement Association during the period of 2012 to 2016 were used in this study. BHBA and Ac concentrations in milk were measured by Fourier transform infrared spectroscopy (FTIR). FPR values were obtained using test day records of fat and protein percentage. EB was calculated using previously developed equation based on parity, lactation week, and milk composition data. Genetic parameters were estimated by restricted maximum likelihood procedure based on repeatability model using Wombat program. Results: Elevated milk BHBA and Ac concentrations were observed during the early lactation under the negative energy balance. Milk FPR tends to decrease with the decreasing ketone body concentrations. Heritability estimates for milk BHBA, Ac, EB, and FPR ranged from 0.09 to 0.14, 0.23 to 0.31, 0.19 to 0.52, and 0.16 to 0.42 respectively at parity 1, 2, 3, and 4. The overall heritability for BHBA, Ac, EB and FPR were 0.29, 0.32, 0.58, and 0.38 respectively. A common pattern was observed in heritability of EB and FPR along with parities. Conclusion: FPR and EB can be suggested as potential predictors for risk of hyperketonemia. The heritability estimates of milk BHBA, Ac, EB, and FPR indicate that the selective breeding may contribute to maintaining the milk ketone bodies at optimum level during early lactation.

Growth and Carcass Characteristics of Goats Given Diets Varying Protein Concentration and Feeding Level

  • Shahjalal, M.;Bishwas, M.A.A.;Tareque, A.M.M.;Dohi, H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.5
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    • pp.613-618
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    • 2000
  • Twelve castrated male Black Bengal goats with an average live weight of 10.3 kg (8 months old) were used in a $2{\times}2$ factorial arrangement to study the effects of dietary crude protein concentration [20.3% (HP) and 16.9% (LP)] and feeding level [ad libitum and 85% of ad libitum (restricted)] on growth and carcass characteristics. Ad libitum feeding, on average, significantly (p<0.05 to p<0.01) increased daily live weight (6.27 vs -5.86 g), dry mater intake (409 vs 351 g/d), estimated gain in carcass (0.195 vs -0.200 kg) and empty body weight (0.385 vs -0.350 kg), chemically extracted fat in meat sample (6.89 vs 6.48%), depth of M. longissimus dorsi (22.1 vs 18.3 mm) and gut and caul fat (170 vs 130 g) compared with restricted feeding regime. The greater intake of dietary protein from the HP diet resulted in significantly (p<0.05 to p<0.01) greater values for depth (20.9 vs 19.5 mm) and width (32.4 vs 27.9 mm) of M. longissimus dorsi, gut and caul fat (190 vs 110 g) and also perirenal and retroperitoneal fat (85 vs 50 g) than those of the goats that received the LP diet. Similarly, the HP diet had significantly (p<0.05 to p<0.01) higher values for CP digestibility and DCP concentration than those of the LP diet. The results indicated that growth rate and carcass gain were highest in goats fed the HP diet ad libitum and therefore, diet containing 20.3% CP may be suggested for feeding growing goats.

Effect of Adrenal Hormones and Diets on Diabetic Rats (부신홀몬과 식이가 당뇨병 백서에 미치는 영향)

  • 주진순
    • Journal of Nutrition and Health
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    • v.22 no.2
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    • pp.63-69
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    • 1989
  • For assessment of the effects of adrenal hormones and diets on diabetic condition, adrenalectomized. Sprague-Dawley rats were fed either high carbohydrate diet, high protein diet, or high fat diet with administration of aldosterone or corticsterone. Adrenalectomy of the rats reduced serum glucos, insulin, total cholesterol, and body weight in all types of diet feeding. In comparison with adrenalectomized control group, corticoste5rone-administered group showed significant increase of serum glucose when fed high fat diet or high protein diet ; however, aldosterone-administered group with high fat diet or high protein diet ; however, aldosterone-administered group with high hat diet showed significant decrease o serum glucose. Serum lipid was increased significantly in cortricosterone-administered group with feeding of high fat or high protein diet. There was no significant effects of aldosterone administeration on parameters determined in this study.

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Pectinase-Processed Ginseng Radix (GINST) Ameliorates Hyperglycemia and Hyperlipidemia in High Fat Diet-Fed ICR Mice

  • Yuan, Hai-Dan;Kim, Jung-Tae;Chung, Sung-Hyun
    • Biomolecules & Therapeutics
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    • v.20 no.2
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    • pp.220-225
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    • 2012
  • To develop a ginseng product possessing an efficacy for diabetes, ginseng radix ethanol extract was treated with pectinase and obtained the GINST. In the present study, we evaluate the beneficial effect of GINST on high fat diet (HFD)-induced hyperglycemia and hyperlipidemia and action mechanism(s) in ICR mice. The mice were randomly divided into five groups: regular diet group (RD), high fat diet group (HFD), HFD plus GINST at 75 mg/kg (GINST75), 150 mg/kg (GINST150), and 300 mg/kg (GINST300). Oral glucose tolerance test reveals that GINST improves the glucose tolerance after glucose challenge. Fasting plasma glucose and insulin levels were decreased by 4.3% and 4.2% in GINST75, 10.9% and 20.0% in GINST150, and 19.6% and 20.9% in GINST300 compared to those in HFD control group. Insulin resistance indices were also markedly decreased by 8.2% in GINST75, 28.7% in GINST150, and 36.4% in GINST300, compared to the HFD control group. Plasma triglyceride, total cholesterol and non-esterified fatty acid levels in the GINST300 group were decreased by 13.5%, 22.7% and 24.1%, respectively, compared to those in HFD control group. Enlarged adipocytes of HFD control group were markedly decreased in GINST-treated groups, and shrunken islets of HFD control mice were brought back to near normal shape in GINST300 group. Furthermore, GINST enhanced phosphorylation of AMP-activated protein kinase (AMPK) and glucose transporter 4 (GLUT4). In summary, GINST prevents HFD-induced hyperglycemia and hyperlipidemia through reducing insulin resistance via activating AMPK-GLUT4 pathways, and could be a potential therapeutic agent for type 2 diabetes.

Effect of Graded Levels of Tallow in the Diet on Performance, Digestibility of Fat, Lipogenesis and Body Lipid Deposition of the Weaned Piglet

  • Reis de Souza, T.C.;Aumaitre, A.;Mourot, J.;Peiniau, J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.4
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    • pp.497-505
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    • 2000
  • Thirty piglets weaned at 24.5 d of age ($6.9{\pm}0.5kg$) randomly alloted to 3 treatments were used to investigate the effect of dietary tallow on average performance, digestibility of nutrients, metabolic utilization of energy and body composition at 25 kg. Weaned piglets respond to increasing levels of dietary tallow from 0 to 4% and 8% by digestive and metabolic adaptation. Apparent fecal digestibility of fat (AFDf) was highly correlated with the level of dietary tallow (X as % of fat extracted after HCl hydrolysis) by the following curvilinear equation of regression: $AFDf=33.8+6.9X-0.3X^2$. Feed intake expressed as DE was only significantly increased at the higher inclusion level of tallow. But neither average daily gain, nor feed conversion was affected by the addition of fat. On the other hand, body composition at 25 kg was equally affected, by both levels of supplementary fat; dry matter and energy content in the body were significantly higher (p<0.01) in piglets receiving tallow. As a consequence, the energy cost of the live weight gain was also increased from 23 to 24.7 MJ DE/kg (p<0.02) and the efficiency of energy deposition was decreased from 3.2 to 2.8 MJ DE/MJ deposited energy (p<0.01) in the presence of dietary tallow. An increase in the level of fat stimulated the activity of pancreatic lipase up to a constant value of $22{\pm}1.4IU/mg$ protein but conversely depressed the activity of amylase from 300 to 100 IU/mg of protein. The activity of liver acetyl CoA carboxylase and malic enzyme in the perirenal fat were low lind not affected by dietary fat; the activity of glucose-6-phosphate dehydrogenase was high. Opposite to that, the activity of acetyl CoA carboxylase and malic enzyme in the perirenal and backfat were higher than in the liver and both were significantly reduced by the inclusion of fat in the diet. A direct deposition of dietary fat has been demonstrated by increasing the energy and lipid content of the empty body weight gain between 7 and 25 kg of live weight, and decreasing the efficiency of digestible energy utilization.