• 제목/요약/키워드: FAMEs

검색결과 41건 처리시간 0.021초

영흥도에서 분리된 Phaeodactylum tricornutum의 증식 및 Monounsaturated fatty acid 관련 지방산 조성 분석 (Investigation of cultivation and FAME composition isolated Phaeodactylum tricornutum from Youngheung island)

  • 이상민;조용희;신동우;전효남;류영진;임상민;이철균
    • 한국해양바이오학회지
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    • 제6권2호
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    • pp.47-52
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    • 2014
  • Oxidation stability and cold fuid property are considered as the most important factors for determining biodiesel quality. Among the fatty acids, monounsaturated fatty acid satisfy both oxidation stability and cold flow property of biodiesel quality standards. Microalgae with high monounsaturated fatty acid contents is have some benefit for producing to produce biodiesels with satisfying quality standards. In this study, monounsaturated fatty acid contents of a isolated microalga from Youngheung island was analyzed. Phaeodactylum tricornutum was isolated by streaking, and growth rate and fatty acid composition of the algae were investigated. Total FAME contents were consisted of 26% of saturated fatty acids, 43% of monounsaturated fatty acids, and 18% of polyunsaturated fatty acids. The contents of monounsaturated fatty acid were especially high in the Phaeodactylum species. This result implies that the FAMEs from P. tricornutum may contribute to improve the oxidation stability and cold flow property of biodiesel.

Complete nucleotide sequence of genome RNA of Daphe virus S and its relationship n the genus Carlavirus (oral)

  • Lee, B.Y.;K.H. Ryu
    • 한국식물병리학회:학술대회논문집
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    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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    • pp.115.2-116
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    • 2003
  • Complete genomic nucleotide sequence of Daphe virus S (DVS), a member of the genus Carlavirus, causing leaf distortion and chlorotic spot disease symptoms in daphne plants, has been determined in this study. The genome of DVS contained six open reading fames coding for long viral replicase, triple gene block, 36 kDa viral coat protein (CP) and 12 kDa from the 5' to 3' ends, which is a typical genome structure of carlaviruses. Two Korean isolates of DVS isolates were 98.1% and 93.6% amino acid identical in the CP and 12kDa, respectively. The CP gene of DVS shares 25.2-55.2% and 42.9-56.1% similarities with that of 19 other carlaviruses at the amino acid and nucleotide levels, respectively. The 3'-proximal 12 kDa gene of DVS shares 20.2-57.8% amino acid identities with that of 18 other members of the genus. The 3' noncoding region of DVS consists of 73 nucleotides with long excluding poly A tract, and shares 69.1-77.1% identities to the known carlaviruses. In the phylogenetic analyses of the two proteins, DVS was closely related to Helenium virus S and Chrysanthemum virus B. This is the first complete sequence information for the DVS, and further confirms the classification of DVS as a distinct species of the genus Carlavirus.

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입술정보를 이용한 입술모양의 기하학적 보정 (Geometric Correction of Lips Using Lip Information)

  • 황동국;박희정;전병민
    • 한국통신학회논문지
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    • 제29권6C호
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    • pp.834-841
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    • 2004
  • 화자의 입술영상에는 카메라와 화자의 위치나 자세로 인하여 정상적인 입술이 기하학적으로 변환될 수 있다. 이러한 변환은 본래 입술위상의 기하학적 정보를 변경시킨다. 따라서 기하학적으로 변환된 입술모양을 보정하는데 부분적인 입술위상정보를 사용함으로써 전체 입술정보를 개선하고자, 본 논문에서는 입술모양의 기하학적 보정 기법을 제안한다. 제안한 기법은 특징결정 단계와 보정 단계로 구성된다. 특징결정 단계에서는 원영상과 목표영상의 입술모델에 따라 원영상의 특징점과 특징을 추출하고 목표영상의 특징점과 특징을 결정한다. 보정단계에서는 이전 단계에서 추출한 정보를 기반으로 영상을 부분영상으로 분할하고 사상 후 보정된 영상을 통합한다. 실험영상은 6개의 한국어 단모음 발음에 대한 동영상 프레임이고 알고리즘 평가를 위하여 입술의 좌우 대칭성을 활용한다. 실험 결과, 윗입술보다는 아랫입술의 보정률, 입술의 움직임이 작은 발음보다는 움직임이 큰 발음의 보정률이 높게 개선되었다.

Identification of an Antagonistic Bacterium, KJ1R5, for Biological Control of Phytophthora Blight of Pepper

  • Kim, Hye-Sook;Myung, Inn-Shik;Kim, Ki-Deok
    • 한국식물병리학회:학술대회논문집
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    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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    • pp.97.1-97
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    • 2003
  • An antagonistic bacterium, KJ1R5,, to Phytophthora capsici was obtained from root interior of a healthy pepper plant. To identify the bacterial antagonist, 16S rDNA sequence analysis, Biolog system, fatty acid methyl-esters (FAMEs), and physiological and biochemical characterization were conducted. The determined 165 rDNA sequence of KJ1R5, showed higher similarities to those of a group consisting of several Chryseobacterium strains with 95.2, 95.2, and 95,1% similarity to C. defluvii, Chryseobacterium sp. FR2, and C. scophthalmum, respectively, In addition, Halounella gailinarum, Bergeyella zoohelcum, and Riemerella anatipestifer are another group for KJ1R5, with 94.1, 89.7, and 87.2% similarities, respectively When identification of the antagonistic bacterium, KJ1R5, was conducted using BIOLOG system, the strain KJ1R5, was identified as Flavobacterium tirrenicum (similarity; 0.75%). Fatty acid profiles of the strain KJ1R5, were composed mainly of iso-17:0 w9c and iso-15:0 and identified as Chryseobacterium balustinum (similarity 0.524%). KJ1R5, was Gram-negative, regular short rods ranging from 0.8 $\mu\textrm{m}$ to 1.0 $\mu\textrm{m}$ and had no flagella. Phenotypic characterization of the antagonistic bacterium indicated that KJ1R5, were included in the genus Chreseobacterium, which belongs to the family Flavobacteriaceae. The strain was distinguished from these six existing species. These results indicated that strain might be placed as a new species in the genus Chryseobacterium.

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지방산에 의한 경지 및 미경지 토양의 미생물군집평가 (Evaluation of Microbial Community Composition in Cultivated and Uncultivated Upland Soils by Fatty Acids)

  • 서장선;전길형;권장식;김상효;백형진
    • 한국토양비료학회지
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    • 제36권4호
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    • pp.239-246
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    • 2003
  • 경지이용이 토양 화학성과 미생물 군집상에 어떠한 영향이 있는지 경지 및 미경지 토양을 채취하여 토양지방산, 미생물밀도 및 미생물체량간(biomass C)의 관계를 조사하였다. 경지와 미경지 토양의 pH는 큰 차이가 없었지만, 전기전도도 (EC), 유기물, 유효인산 및 치환성 양이온 함량은 미경지 토양에 비해 경지토양에서 높았다. 훈증추출법에 의해 측정된 미생물체량과 지방산 총 함량간에는 유의한 정의 상관관계가 있었다 ($r^2=0.557$, n=18, p<0.01). 세균, 방선균, 사상균, 원생동물을 나타내는 지표성 지방산 함량은 경지토양에서 높은 경향을 보였다.

Effects of Carbon and Nitrogen Sources on Fatty Acid Contents and Composition in the Green Microalga, Chlorella sp. 227

  • Cho, Sun-Ja;Lee, Duk-Haeng;Luong, Thao Thanh;Park, So-Ra;Oh, You-Kwan;Lee, Tae-Ho
    • Journal of Microbiology and Biotechnology
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    • 제21권10호
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    • pp.1073-1080
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    • 2011
  • In order to investigate and generalize the effects of carbon and nitrogen sources on the growth of and lipid production in Chlorella sp. 227, several nutritional combinations consisting of different carbon and nitrogen sources and concentrations were given to the media for cultivation of Chlorella sp. 227, respectively. The growth rate and lipid content were affected largely by concentration rather than by sources. The maximum specific growth was negatively affected by low concentrations of carbon and nitrogen. There is a maximum allowable inorganic carbon concentration (less than 500~1,000 mM bicarbonate) in autotrophic culture, but the maximum lipid content per gram dry cell weight (g DCW) was little affected by the concentration of inorganic carbon within the concentration. The lipid content per g DCW was increased when the microalga was cultured with the addition of glucose and bicarbonate (mixotrophic) at a fixed nitrogen concentration and with the lowest nitrogen concentration (0.2 mM), relatively. Considering that lipid contents per g DCW increased in those conditions, it suggests that a high ratio of carbon to nitrogen in culture media promotes lipid accumulation in the cells. Interestingly, a significant increase of the oleic acid amount to total fatty acids was observed in those conditions. These results showed the possibility to induce lipid production of high quality and content per g DCW by modifying the cultivation conditions.

VPN을 적용한 인터넷 전화 단말기의 성능평가에 관한 연구 (Study on Eveluation of Performancen on Internet Phone(VoIP) using the VPN)

  • 이성기;유승선;이명재;곽훈성
    • 한국통신학회논문지
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    • 제30권6A호
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    • pp.445-454
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    • 2005
  • 암호화된 인터넷 전화 단말기의 통화품질 측정을 하기위해 실험환경을 구성하여 캡슐화에 따른 지연을 실험한 결과 구현 인터넷 전화기와 일반 VoIP 전화기와의 지연시간의 차이는 최대 5ms 이하였다. 이러한 지연시간의 대부분은 VPN의 터널링을 위한 패킷 캡슐화에 소요되는 지연시간으로 예상할 수 있다. 따라서 일반 VoIP 전화기와 본 연구에서 제시한 VPN을 적용한 VoIP 전화기와의 통화품질의 차이는 무시할 만하였다. 또한 하나의 패킷당 프레임 개수를 다르게 하여 통신하는 동안 네트워크 전체 패킷의 크기를 구하여, PAC과 PNS사이의 캡슐화 과정이 네트워크 부하에 가장 많은 영향을 주는 요소로 작용한다. 따라서 본 연구에서는 최적의 패킷 당 프레임 수를 산출하기 위해 패킷에 할당 하는 프레임 개수를 증가시켜 통화 품질에 문제가 없는 종 단간 지연을 150ms 이내로 할 때, 패킷 당 프레임 개수는 $2{\sim}3$개가 적합하다는 결론을 얻었다.

미국(美國) Sportswear에 관한 연구(硏究) - Claire McCardell, Cavin Klein, Ralph Lauren, Donna Karan을 중심으로 - (The Research for Amarican Sportswear - Focusing on Claire McCardell, Calvin Klein, Ralph Lauren and Donna Karan -)

  • 정성혜
    • 복식
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    • 제15권
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    • pp.217-231
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    • 1990
  • The purposes of this study were to research 1) the background of origination, 2) the process of development and 3) the influences to modern clothing culture of American sportswear. The most Influential sportswear designers in America from beginning period to present : such as Claire McCardell, Calvin Klein, Ralph Lauren and Donna Karan were selected to study their life and achievements in the world sportswear market. The Results were as follows : 1) World War I & II have caused the greatest changes to modern history America became the center of world economy, society and culture. Politically, they have influenced democracy world-wide and raised the social status of women. The free American women enjoyed sports, Jazz drinking and car-driving. The advanced technology have accelerated mechanization and mass-production to the fashion industrv. 2) The above historical backgrounds have caused to change women's clothing to casual, practical and simple form. Dorothy Shaver at Lord & Taylor began promoting American designers who created the original American spirit. Claire McCardell created the seperated American sportswear to become world-wide fashion. Calvin Klein and Ralph Lauren, the sportswear designers who have the unique American feeling have come to have riches and fames equal to the top designers in Paris. 3) The American sportswear and fashion industry influenced Paris Mode, the traditional Haute Couture to open Pret-a-porter-a-porter. The concept of seperated coordination in American sportswear have promoted the advanced marketing techniques and merchandising systems to modern ready-to-wear industry. Fashion in 1980s have increased the mutual interdepedence between the American sportswear industry and the creative Paris Mode. And then, the fashion world in 1990s and 21C will be changed and developed on the basis of American sportswear mechanism.

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수환경 녹농균의 세포막 지방산 유사도 분석에 의한 Subgrouping과 활용 (Subgrouping of Pseudomonas aeruginosa Isolates by Resemblance Coefficiency of the Cellular Fatty Acid Analysis)

  • 정현미;김동빈
    • 미생물학회지
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    • 제38권4호
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    • pp.276-280
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    • 2002
  • 본 연구는 수환경이나 먹는샘물 생산 공장 등에서 녹농균이 검출되었을 경우 오염원추적의 1차 조사수단으로 세포막 지방산의 유사도 분석 활용 가능성을 평가함을 목적으로 하였다. 이를 위하여 54개 수환경 분리 균주, 3개 표준 균주, 토양과 분변 분리 균주 4종 등 총 61 개 녹농균의 세포 지방산을 분석하였고, 유사도를 산정하여 가계도식 도표(dendrogram)로 나타내었다. 그 결과 61개 균주 모두가 ED (Euclidian Distance) 8.4에서 단일한 cluster로 grouping되었고 ED 6.9에서 4개의 subgroups들로 분류되었다. 그 subgroups들은 현장 분리, 외국의 표준 균주 등 기원에 따라 각각의 subgroup으로 분류되어 미생물 동정에 사용되는 세포 지방산 분석의 유사도 산출이 먹는샘물 공장 등 수환경에서 경제적이고 간단한 오염원 조사의 1차 수단으로서 활용 될 가능성을 보여주었다.

김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

  • 이정숙;정민철;김우식;이근철;김홍중;박찬선;이헌주;주윤정;이근종;안종석;박완;박용하;민태익
    • 한국미생물·생명공학회지
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    • 제24권2호
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    • pp.234-241
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    • 1996
  • Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were used for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined at Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), the genus Lactobacillus (aggregate group F), the genera Lactobacillus and Pediococcus (aggregate group E) and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacteria from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

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