• Journal of Marine Bioscience and Biotechnology
• /
• v.6 no.2
• /
• pp.47-52
• /
• 2014

Oxidation stability and cold fuid property are considered as the most important factors for determining biodiesel quality. Among the fatty acids, monounsaturated fatty acid satisfy both oxidation stability and cold flow property of biodiesel quality standards. Microalgae with high monounsaturated fatty acid contents is have some benefit for producing to produce biodiesels with satisfying quality standards. In this study, monounsaturated fatty acid contents of a isolated microalga from Youngheung island was analyzed. Phaeodactylum tricornutum was isolated by streaking, and growth rate and fatty acid composition of the algae were investigated. Total FAME contents were consisted of 26% of saturated fatty acids, 43% of monounsaturated fatty acids, and 18% of polyunsaturated fatty acids. The contents of monounsaturated fatty acid were especially high in the Phaeodactylum species. This result implies that the FAMEs from P. tricornutum may contribute to improve the oxidation stability and cold flow property of biodiesel.

• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 2003.10a
• /
• pp.115.2-116
• /
• 2003

Complete genomic nucleotide sequence of Daphe virus S (DVS), a member of the genus Carlavirus, causing leaf distortion and chlorotic spot disease symptoms in daphne plants, has been determined in this study. The genome of DVS contained six open reading fames coding for long viral replicase, triple gene block, 36 kDa viral coat protein (CP) and 12 kDa from the 5' to 3' ends, which is a typical genome structure of carlaviruses. Two Korean isolates of DVS isolates were 98.1％ and 93.6％ amino acid identical in the CP and 12kDa, respectively. The CP gene of DVS shares 25.2-55.2％ and 42.9-56.1％ similarities with that of 19 other carlaviruses at the amino acid and nucleotide levels, respectively. The 3'-proximal 12 kDa gene of DVS shares 20.2-57.8％ amino acid identities with that of 18 other members of the genus. The 3' noncoding region of DVS consists of 73 nucleotides with long excluding poly A tract, and shares 69.1-77.1％ identities to the known carlaviruses. In the phylogenetic analyses of the two proteins, DVS was closely related to Helenium virus S and Chrysanthemum virus B. This is the first complete sequence information for the DVS, and further confirms the classification of DVS as a distinct species of the genus Carlavirus.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.29 no.6C
• /
• pp.834-841
• /
• 2004

There can be lips transformed geometrically in the lip images according to the location or the pose of camera and speaker. This transformation of the lip images changes geometric information of original lip phases. Therefore, for enhancing global lip information by using partial information of lips to correct lip phases transformed geometrically, in this paper we propose a method that can geometrically correct lips. The method is composed of two steps - the feature-deciding step and the correcting step. In the former, it is for us to extract key points and features of source image according to the its lip model and to create that of target image according to the its lip model. In the latter, we decide mapping relation after partition a source and target image based on information extracted in the previous step into each 4 regions. and then, after mapping, we unite corrected sub-images to a result image. As experiment image, we use fames that contain pronunciation on short vowels of the Korean language and use lip symmetry for evaluating the proposed algorithm. In experiment result, the correcting rate of the lower lip than the upper lip and that of lips moving largely than little was highly enhanced.

• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 2003.10a
• /
• pp.97.1-97
• /
• 2003

An antagonistic bacterium, KJ1R5,, to Phytophthora capsici was obtained from root interior of a healthy pepper plant. To identify the bacterial antagonist, 16S rDNA sequence analysis, Biolog system, fatty acid methyl-esters (FAMEs), and physiological and biochemical characterization were conducted. The determined 165 rDNA sequence of KJ1R5, showed higher similarities to those of a group consisting of several Chryseobacterium strains with 95.2, 95.2, and 95,1％ similarity to C. defluvii, Chryseobacterium sp. FR2, and C. scophthalmum, respectively, In addition, Halounella gailinarum, Bergeyella zoohelcum, and Riemerella anatipestifer are another group for KJ1R5, with 94.1, 89.7, and 87.2％ similarities, respectively When identification of the antagonistic bacterium, KJ1R5, was conducted using BIOLOG system, the strain KJ1R5, was identified as Flavobacterium tirrenicum (similarity； 0.75％). Fatty acid profiles of the strain KJ1R5, were composed mainly of iso-17：0 w9c and iso-15：0 and identified as Chryseobacterium balustinum (similarity 0.524％). KJ1R5, was Gram-negative, regular short rods ranging from 0.8 $\mu\textrm{m}$ to 1.0 $\mu\textrm{m}$ and had no flagella. Phenotypic characterization of the antagonistic bacterium indicated that KJ1R5, were included in the genus Chreseobacterium, which belongs to the family Flavobacteriaceae. The strain was distinguished from these six existing species. These results indicated that strain might be placed as a new species in the genus Chryseobacterium.

• Korean Journal of Soil Science and Fertilizer
• /
• v.36 no.4
• /
• pp.239-246
• /
• 2003

We examined the relationships among community composition, microbial population, and microbial biomass to determine whether different land use leads to differences in microbial community composition. And also the relationships between soil characteristics and microbial community composition were investigated. There was no difference in pH between uncultivated and cultivated soils, but electrical conductivity, and contents of organic matter, available P and exchangeable cations were greater in the cultivated soil compared to the uncultivated soil. A linear correlation ($r^2=0.557$, n=18, p<0.01) was found between biomass-C estimated with fumigation extraction technique and total amount of fatty acids. An increase of fatty acid methyl esters (FAMEs) for bacteria, actinomycetes, fungi and protozoa was observed in cultivated soil.

• Journal of Microbiology and Biotechnology
• /
• v.21 no.10
• /
• pp.1073-1080
• /
• 2011

In order to investigate and generalize the effects of carbon and nitrogen sources on the growth of and lipid production in Chlorella sp. 227, several nutritional combinations consisting of different carbon and nitrogen sources and concentrations were given to the media for cultivation of Chlorella sp. 227, respectively. The growth rate and lipid content were affected largely by concentration rather than by sources. The maximum specific growth was negatively affected by low concentrations of carbon and nitrogen. There is a maximum allowable inorganic carbon concentration (less than 500~1,000 mM bicarbonate) in autotrophic culture, but the maximum lipid content per gram dry cell weight (g DCW) was little affected by the concentration of inorganic carbon within the concentration. The lipid content per g DCW was increased when the microalga was cultured with the addition of glucose and bicarbonate (mixotrophic) at a fixed nitrogen concentration and with the lowest nitrogen concentration (0.2 mM), relatively. Considering that lipid contents per g DCW increased in those conditions, it suggests that a high ratio of carbon to nitrogen in culture media promotes lipid accumulation in the cells. Interestingly, a significant increase of the oleic acid amount to total fatty acids was observed in those conditions. These results showed the possibility to induce lipid production of high quality and content per g DCW by modifying the cultivation conditions.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.30 no.6A
• /
• pp.445-454
• /
• 2005

To measure the performance of call quality, we have built the experiment environment and observed that the delay caused by encapsulation between internet and VoIP telephones is under 5ms at most. The major delay is assumed to be the time required to capsulate the packet for tunnelling of VPN. Because the difference of average delay time is under $4ms{\sim}5ms$, the difference of call quality between VoIP and VoIP telephone adopting VPN is negligible. We have concluded that the capsulation process between PAC and PNS is the major factor influencing the network load by changing the number of fames in a packet during communication Also, we have concluded that the most suitable frame numbers is tow or three by adding the frame numbers in a packet to obtain the suitable frames in a packet and setting up end-to-end delay under 150ms.

• Journal of the Korean Society of Costume
• /
• v.15
• /
• pp.217-231
• /
• 1990

The purposes of this study were to research 1) the background of origination, 2) the process of development and 3) the influences to modern clothing culture of American sportswear. The most Influential sportswear designers in America from beginning period to present : such as Claire McCardell, Calvin Klein, Ralph Lauren and Donna Karan were selected to study their life and achievements in the world sportswear market. The Results were as follows : 1) World War I & II have caused the greatest changes to modern history America became the center of world economy, society and culture. Politically, they have influenced democracy world-wide and raised the social status of women. The free American women enjoyed sports, Jazz drinking and car-driving. The advanced technology have accelerated mechanization and mass-production to the fashion industrv. 2) The above historical backgrounds have caused to change women's clothing to casual, practical and simple form. Dorothy Shaver at Lord & Taylor began promoting American designers who created the original American spirit. Claire McCardell created the seperated American sportswear to become world-wide fashion. Calvin Klein and Ralph Lauren, the sportswear designers who have the unique American feeling have come to have riches and fames equal to the top designers in Paris. 3) The American sportswear and fashion industry influenced Paris Mode, the traditional Haute Couture to open Pret-a-porter-a-porter. The concept of seperated coordination in American sportswear have promoted the advanced marketing techniques and merchandising systems to modern ready-to-wear industry. Fashion in 1980s have increased the mutual interdepedence between the American sportswear industry and the creative Paris Mode. And then, the fashion world in 1990s and 21C will be changed and developed on the basis of American sportswear mechanism.

• Korean Journal of Microbiology
• /
• v.38 no.4
• /
• pp.276-280
• /
• 2002

To find the applicability of resemblance of the fatty acid methy1 ester (FAME) profile analysis to the primary examination for the tracing P. aeruginosa contamination in the environment and water plants, we designed this study. Of6l P. aeruginosa isolates including 3 reference strains, 4 fecal or soil isolates and 54 water isolates, the resemblance coefficiencies of the FAMEs were calculated as euclidian distance (ED) and expressed as dendrogram. The 61 strains were clustered as the same group in the ED 8.4, and divided into 4 subgroups in the ED 6.9. The water isolates from Han River were classified into distinctively different subgroups from reference strains. These results provide the possibility of the dendrogram from FAME profile as a useful tools for tracing contamination as well as for identifying the species, especially in case of P. aeruginosa.

• Microbiology and Biotechnology Letters
• /
• v.24 no.2
• /
• pp.234-241
• /
• 1996

Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were used for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined at Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), the genus Lactobacillus (aggregate group F), the genera Lactobacillus and Pediococcus (aggregate group E) and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacteria from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.