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Re-identification of Fusarium sambucinum Species Complex Strains in Korea and Their Literature Review (국내에서 분리된 Fusarium sambucinum 종복합체 균주의 재동정 및 문헌 고찰)

  • Yunhee Choi;Anbazhagan Mageswari;Hyorim Choi;Jisu Lee;Daseul Lee;Seung-Beom Hong
    • Research in Plant Disease
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    • v.29 no.2
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    • pp.118-129
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    • 2023
  • Fusarium sambucinum species complex (FSAMSC) is an important taxonomic group, causing severe plant diseases. Many studies were carried out on FSAMSC plant diseases in Korea, but only 2 species (F. graminearum, F. sambucinum) from 14 host plants were registered in the List of Plant Disease in Korea. To clarify FSAMSC diversity and their pathogenecity, we examined FSAMSC isolates preserved in the Korean Agricultural Culture Collection. Fifty-seven strains were reidentifed as 7 species (F. asiaticum, F. graminearum, F. vorosii, F. meridionale, F. boothii, F. kyushuense, F. armeniacum) based on multi-locus sequence typing analysis. According to previous reports and result of this study, 5 species (F. asiaticum, F. graminearum, F. vorosii, F. armeniacum, F. sambucinum) were pathogenic on 24 host plants in FSAMSC, while the pathogenicity of 3 species (F. meridionale, F. boothii, F. kyushuense) were not clear.

Basic Studies on the Consumptive Use of Water Required for Dry Field Crops -Tomato and Chinese Cabbage- (밭작물소비수량에 관한 기초적 연구 -토마토 및 가을배추-)

  • 김철기;김진한;최홍규
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.30 no.3
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    • pp.25-37
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    • 1988
  • The purpose of this study is to fmd out the bask data for irrigation plans of tomato and chinese cabbage during the growing period, such as total amount of evapotranspiration, coefficients of evapotranspiration at each growth stage, the peak stage of evapotranspiration, the maximum evapotranspiration, optimum irrigation point, total readily available moisture and intervals of irrigation date. The plots of experiment were arranged with split plot design which were composed of two factors, irrigation point for main plot and soji texture for split plot, and three levels, irrigation points with PF 1.8, PF 2.2, PF 2.6 for tomato and those with PF 1.9, PF 2.3, PF 2.7, for Chinese cabbage, soil textures of silty clay, sandy loam and sandy soil for both tomato and Chinese cabbage, with two replications. The results obtained are summarized as follows 1. There was the highest significant correlation between the evapotranspiration and the pan evaporation, beyond all other meteoralogical factors considered. Therefore, the pan evaporation is enough to be used as a meteorological index measuring the quantity of evapotranspiration. 2. 1/10 probability values of maximum total pan evaporation during growing period for tomato and Chinese cabbage were shown as 355.8 mm and 233.0 mm, respectively, and those of maximum ten day pan evaporation for tomato and Chinese cabbage, 68.0 mm and 43.8 mm, respectively. 3. The time that annual maximum of ten day pan evaporation can be occurred, exists at any stage of growing period for tomato, and at any growth stage till the late of Septemberfor Chinese cabbage. 4. The magnitude of evapotranspiration and of its coefficient for tomato and Chinese cabbage was occurred in the order of pF 1.8>pF 2.2>pF 2.6 and of pF 1.9>pF 2.3>pF 2.7 respectively in aspect of irrigation point and of silty clay>sandy loam>sandy soil in aspect of soil texture. 5. 1/10 probability value of evapotranspiration and its coefficient during the growing period of tomato were shown as 327.3 mm and 0.92 respectively, while those of Chinese cabbage, 261.0 mm and 1.12 respectively. 6. The time that maximum evapotranspiration of tomato can be occurred is at the date of fortieth to fiftieth after transplanting and the time for Chinese cabbage is presumed to he in the late of septemben At that time, 1/10 probability value of ten day evapotranspiration and its coefficient for tomato is presumed to be 74.8 mm and 1.10 respectively, while those of Chinese cabbage, 43.8 mm and 1.00. 7. In aspect of only irrigaton point, the weight of raw tomato and Chinese cabbage were mcreased in the order of pF 2.2>pF 1.8>pF 2.6 and of pF 1.9>pF 2.3>pF 2.7, respectively but optimum irrigation point for tomato and Chinese cabbage, is presumed to be pF 2.6 - 2.7 if nonsignificance of the yield between the different irrigation treatments, economy of water, and reduction in labour of irrigaion are synthetically considered. 8. The soil moisture extraction patterns of tomato and Chinese cabbage have shown that maximum extraction rate exists at 7 cm deep layer at the beginning stage of growth m any soil texture and that extraction rates of 21 cm to 35 cm deep layer are increased as getting closer to the late stage of growth. And especially the extraction rates of 21 cm deep layer and 35 cm deep layer have shown tendency to be more increased in silty clay than in any other soils. 9. As optimum irrigation point is presumed to be pF Z6-2.7, total readily available moisture of tomato in silty clay, sandy loam and sandy sofl becomes to be 19.06 mm, 21.37 mm and 20.91 mm respectively while that of Chinese cabbage, 18.51 mm, 20.27 mm, 21.11 mm respectively. 10. On the basis of optimum irrigation point with pF 2.6 - 2.7 the intervals of irrigation date of tomato and Chinese cabbage at the growth stage of maximum consumptive use become to be three days and five days respectively.

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Cloning and Sequence Analysis of the trpB, trpA and 3' trpC(F) Gens of Vibrio metschnikovii Strain RH530 (Vibrio metschnikovii 균주 RH530의 trpB, trpA 그리고 3' trpC(F) 유전자의 클로닝 및 염기서열 결정)

  • Kwon, Yong-Tae;Kim, Jin-Oh;Yoo, Young-Dong;Rho, Hyune-Mo
    • Korean Journal of Microbiology
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    • v.32 no.2
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    • pp.120-125
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    • 1994
  • The genes, trpB, trpA and 3’ trpC(F) of Vibrio metschnikovii strain RH530 were cloned and sequenced. The trpB and trpA genes had open reading frames of 1,173 bp and 804 bp encoding 391 and 268 amino acids, respectively. The trpB and trpA genes had conventional ribosome-binding sequences and overlapped with each other by one nucleotide, suggesting that these two genes are translationally coupled. 115 nucleotide upstream the trpB start codon, tjere was an incomplete open reading frame of the 3’-end of the trpC(F). The amino acid sequences of trpB, trpA and trpC(F) of V. metschnikovii RH530 had identities of 64.2%, 82.4% and 73.7% respectively, for those of V. parahaemolyticus; 58.7%, 72.3% and 54.9%, respectively, for Salmonella typhimurium; and 42.6%. 54.1% and 12.5%, respectively, for brevibacterium lactofermentum. The genetic organization of these genes, especially in the noncoding region between trpC(F) and trpB, was distinct from that of Enterobacteriaceae.

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A Study on Worker Exposure Level and Variation to Asbestos in some Asbestos Industries (일부 석면취급사업장의 석면폭로 농도 및 작업환경관리 기준에 관한 연구)

  • Oh, Se Min;Shin, Yong Chul;Park, Doo Yong;Park, Dong Uk;Chung, Kyou Chull
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.3 no.1
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    • pp.100-109
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    • 1993
  • This study was performed to evaluate the asbestos exposure levels and variations in textile, brake lining manufacturing and slate manufacturing industries. For this study, fifteen plants of brake lining manufacturing industry, 7 plants of textile industry, and 2 plants of slate manufacturing industry were selected and surveyed. Geometric means (GMs) of airborne asbestos concentrations in textile, brake lining manufacturing, and slate manufacturing industries were 1.42 f/cc(0.07-6.1O f/cc), 0.19 f/cc(<0.01-2.67 f/cc) and 0.08 f/cc(0.02-0.67 f/cc), respectively. In textile industry overall GMs of airborne asbestos concentrations in plants with less than 50 workers and in plants with more than 50 workers were 1.60 f/cc and 0.3 f/cc, respectively. Therefore, the size of plant showed some difference in the airborne asbestos concentrations. Three out of 7(42.9%) exceed the Korean standard, 2 f/cc, and every plant exceed the USA standard, 0.2 f/cc of the OSHA-Permissible Exposure Level(OSHA-PEL). Especially, one plant showed the highest average concentration of 2.87 f/cc. In brake lining manufacturing industry, the plants with less than 50 workers showed 0.22 f/cc. The plants with more than 50 workers showed 0.18 f/cc. All plants showed the exposure level below the Korean standard. Five of 15 (33.3%) were above the OSHA-PEL. One plant showed the highest average concentraton of 0.84 f/cc. In slate manufacturing industry, the average exposure level was 0.08 f/cc, and all of the plants were below the Korean standard and the OSHA-PEL.

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Study on Estimation and Application of the Fwl-D-F curves for Urban Basins (도시유역의 Fwl-D-F 곡선 산정 및 활용에 관한 연구)

  • Choi, Hyun-Il;Kim, Eung-Seok
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.11 no.7
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    • pp.2687-2692
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    • 2010
  • There have been performed many researched for flood magnitude analysis, for example, the Flood-Duration-Frequency relations in the west. Because flood water stage data are more available rather than flood amount data at flood gauge stations of Korea, this study developed Flood water level-Duration-Frequency (Fwl-D-F) curves using rainfall Intensity-Duration-Frequency(I-D-F) curves for the quantitative flood risk assessment in urban watersheds. Fwl-D-F curve is made from water level data for 18 years at Joongrayng bridge station of Joongrayng River basin in Han River drainage area. Fwl-D-F curve can estimate the occurrence frequency for a certain flood elevation, which can be used for urban flood forecasting. It is expected that the flood elevation can be estimated from the forecasted rainfall data using both Fwl-D-F and I-D-F curves.

Usefulness of $^{18}F$-FDG Fusion PET for Diagnosing an Early Stage Cancer (조기 암 진단을 위한 $^{18}F$-FDG Fusion PET의 유용성)

  • Jeon, Jae-Hwan;Kim, Byeong-Jin;Lee, Hong-Jae;Kim, Jin-Eui;Kim, Hyun-Joo
    • The Korean Journal of Nuclear Medicine Technology
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    • v.14 no.2
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    • pp.145-149
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    • 2010
  • Purpose: Recently, $^{18}F$-FDG Fusion PET which has a high sensitivity for diagnosing cancer is being used for purpose of health examination. This study is to demonstrate that $^{18}F$-FDG fusion PET study is useful for diagnosing an early stage cancer. Materials and Methods: This research has been conducted with 2790 patients visited Seoul National University Hospital Healthcare System (SNUHHS) for $^{18}F$-FDG fusion PET study for a health examination from February, 2004 to December 2008. PET/CT images were acquired from skull base to femur after 1 hour from injecting $^{18}F$-FDG 0.14 mCi/kg to the patients. GEMINI GS (Philips, Netherlands) was used for scanning. Results: From February 2004 to December 2008, $^{18}F$-FDG Fusion PET study was performed for 99,009 patients among all patients who visited SNUHHS and 2,790 patients was performed. Diagnostic rate for malignant cancer was 0.95% for the patients who were not examined by $^{18}F$-FDG Fusion PET study. 1.94% was for the patients who were. The rate of malignant tumor was showed 10% and benign tumor was 90% among 542 patients who showed abnormality in the PET/CT images. Types and rates of malignant tumor showed thyroid cancer: 31.5%, lung cancer: 14.8%, stomach cancer: 9.3%, rectum cancer: 3.7%, breast cancer: 3.7%, metastasis cancer: 16.7%. Nonspecific lymph node in the mediastinum, physiologic uptake in the colon, diffuse mild hypermetabolism in bilateral thyroid gland were shown as a benign tumor. Conclusion: The diagnostic rate of malignant tumor with $^{18}F$-FDG Fusion PET for a purpose of health examination was relatively higher than general medical examination. Consequently, it is superior and useful for applying $^{18}F$-FDG Fusion PET study for health examination.

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Isolation of the Anti-tumor Promoters from Citrus Peels (감귤과피로부터 발암 promotion 억제활성성분의 분리)

  • Yoon, Chang-Hoon;Jwa, Seung-Mi
    • Applied Biological Chemistry
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    • v.49 no.1
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    • pp.25-29
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    • 2006
  • This study was carried out to isolate the possible anti-tumor promoters from the citrus peel (Citrus natsudaidai Hayata). We fractionated the cold-pressed oil of citrus peel by column chromatography, HPLC and TLC. The analysis on column chromate-graphy yielded seven peaks $(F-I{\sim}F-VII)$, all of which showed single spot on TLC analysis ($R_f$ for $F-I{\sim}VIII$; 0.31, 0.13, 0.13, 0.78, 0.79, 0.69 and 0.84). Among the seven fractions, three fractions (F-I, -II and F-IV) were re-analyzed on HPLC, also showing single peak except for one fraction (F-IV) which was divided two peaks. The retention times $(R_f)$ of F-I and F-II was 3 min. and 2.5 min., respectively, but these of two peaks from F-IV were 2 min. and 4.5 min., respectively. Since the area of the latter peak (4.5 min.) was very smaller than that of the former one (2 min.), it is considered that the latter one did not appear on TLC analysis. The inhibitory effect on tumor promoter 12-O-tetradecanoylphorbol-13-acetate(TPA)-induced Epstein-Barr virus activation in Raji cells was tested for the seven fraction obtained. It decreased in order of F-VI (82.3+1.3%) > F-I (80.4+1.6%) > F-II (77.2+0.9%) > F-III (75.0+1.2%) > F-IV (74.1=1.0%) > F-V (71.0+1.1%) > F-VII (70.2+1.2%). These results imply that some constiuents contained in citrus peels have the inhibitory activity of TPA-induced tumor promotion.

Bioconversion of Ginsenosides by Bifidobacterium CBT BG7, BR3 and BL3 (비피도박테리움 CBT BG7, BR3, BL3의 진세노사이드 전환능)

  • Jiwon Choi;Chang Kwon;Jong Won Kim;Myung Jun Chung;Jong Hyun Yoon;Sanghyun Lim
    • Microbiology and Biotechnology Letters
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    • v.50 no.3
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    • pp.395-403
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    • 2022
  • In this study, we identified that the fermentation of Korean indigenous probiotics and red ginseng produced ginsenoside compound K (CK) from major ginsenosides. Based on whole genome sequencing of 19 probiotics species, β-glucosidase, α-arabinofuranosidase, β-xylosidase, and α-rhamnosidase related to bioconversion of ginsenosides are identified in the genome of 19 species, 3 species, 6 species, and 8 species, respectively. Among the 19 probiotics species, Bifidobacterium longum CBT BG7 converted from ginsenoside Rb1 to CK, and both B. breve CBT BR3 and B. lactis CBT BL3 converted ginsenoside Rb1 to Rd. The final concentration and yield of ginsenoside F2 and CK were higher in the fermentation with the nondisrupted cells than with disrupted cells. The combination of both CBT BG7 and BL3, and CBT BG7 and BR3 showed higher amounts of F2 than CBT BG7 only. CBT BG7 with adding α-amylase increased the amounts of F2. In this study, we identified that the fermentation of both Korean indigenous probiotic bacteria CBT BG7, BR3 and BL3, and red gingseng is able to produce CK, a bioactive compound that promotes health benefits.

Crystal Structure of 1-Cyclopropyl-7-(2,7-diazabicyclo[3.3.0]oct-4-en-7-yl)-6-fluoro-8-methoxy-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (HCI salt) (1-Cyclopropyl-7-(2,7-diazabicyclo[3.3.0]oct-4-en-7-yl)-6-fluoro-8-methoxy-4-oxo-1,4-dihydroquinoline-3-carboxylic acid 염산염의 결정구조)

  • 김문집;신준철
    • Korean Journal of Crystallography
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    • v.6 no.2
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    • pp.103-110
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    • 1995
  • The crystal structure of 1-Cyclopropyl-7-(2,7-diazabicyclo[3.3.0]oct-4-en-7-yl)-6-fluoro-8-methoxy-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (HCI salt) has been determined from single crystal x-ray diffraction study ; C20H21N3O4FCl, Monoclinic, C2/c, a=28.349(2)Å, b=11.941(2)Å, c=12.806(2)Å, β=96.428(9)°, V=4307.8Å3, T=296(2)K, Z=8, CuKα(λ=1.5418Å). The molecular structure was solved by direct method and refined by full-matrix least squares to a final R=4.96% for 2258 unique observed F0>4σ(F0) reflections and 293 parameters. The conformation of the molecule is stabilized by an intramolecular O(28)-H(28)…O(25) [2.517(4)Å, 156.7(447)°] hydrogen bond. Intermoleculars distances correspond to van der Waals contacts.

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