• Animal cells and systems
• /
• v.9 no.2
• /
• pp.87-94
• /
• 2005

Spindle position checkpoint monitors the orientation of mitotic spindle for proper segregation of replicated chromosomes into mother cell and the daughter, and prohibits mitotic exit when mitotic spindle is misaligned. BUB2 forms one of the key upstream element of spindle position checkpoint in budding yeast, but its functional homologues have not been identified in higher eukaryotes. Here, we analyzed the functions of two putative BUB2 homologues of C. elegans in the spindle orientation checkpoint. From the C. elegans genome database, we found that two open reading frames (ORFs), F35H12_2 and C33F10_2, showed high sequence homology with BUB2. We obtained the expressed sequence tag (EST) clones for F35H12_2 (yk221d4) and C33F10_2 (yk14e10) and verified the full cDNA for each ORF by sequencing and 5' RACE with SL1 primer. The functional complementation assays of yk221d4 and yk14e10 in ${\Delta}bub2$ of S. cerevisiae revealed that these putative BUB2 homologues of C. elegans could not replace the function of BUB2 in spindle position checkpoint and mitotic exit. Our attempt to document the component of spindle position checkpoint in metazoans using sequence homology was not successful. This suggests that structural information about its components might be required to identify functional homologues of the spindle position checkpoint in higher eukaryotes.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
• /
• v.24 no.2
• /
• pp.35-40
• /
• 2011

Objective : The aim of this study is to investigate the characteristics of Ryodoraku in the patients with acute and chronic urticaria, and to compare the acute and chronic urticaria. Methods : Subjects were 124 patients with urticaria, acute(<8 weeks) patients were thirty eight and chronic(${\geq}$ 8 weeks) patients were sixty four. We calculated the average Ryodoraku score(RS, ${\mu}A$) and each variation from physiologic range of 12 Ryodoraku points, and compared the acute and chronic urticaria Ryodoraku points pattern. Results : 1. The mean value of F3 and F5 were under 40${\mu}A$ in acute urticaria group. 2. The mean value of H1, H2, H4, H5, H6, F1 and F4 were over 60${\mu}A$ in chronic urticaria group. 3. There were significant difference between acute and chronic urticaria group at H2, H3, H4, F1, F2, F3, F4, F5 and total Ave. Conclusion : These findings suggest that there are significant differences of Ryodoraku pattern between acute and chronic urticaria group, and Ryodoraku reflect the characteristic of urticaria.

• Archives of Pharmacal Research
• /
• v.12 no.1
• /
• pp.52-57
• /
• 1989

The structure of cinmetacin was determined by single crystal X-ray diffraction analysis. The compound was recrystallized from a mixture of acetone and water in orthorhombic, space group $P2_12_12_1$, with Z=4, a=35.681(8), b=9.482(2), c:5.071(1) ${\AA}$, $D_x=1.352 g/cm^3$, and $D_m=1.35g/cm^3$. The structure was solved by direct method and refined by least-squares procedure to the final R value of 0.036 for 1441 observed reflections ($F{\geq}3{\sigma}(F)$). The carboxyl group of the molecule is nearly perpendicular to the indole ring. The dihedral angle between indole ring and phenyl group is $64.5^{\circ}$. The molecules are linked together via O(1)-H ----O(3) hydrogen bonds, and arranged along 2-fold screw axis in the crystal. The intermolecular contacts are the normal van der Waals' forces.

• Phonetics and Speech Sciences
• /
• v.12 no.3
• /
• pp.1-14
• /
• 2020

Previous studies have confirmed that /o/ and /u/ in Seoul Korean are undergoing a merger in the F1/F2 space, especially for female speakers. As a substitute parameter for formants, it is reported that female speakers use phonation (H1-H2) differences to distinguish /o/ from /u/. This study aimed to explore whether H1-H2 values are being used as perceptual cues for /o/-/u/. A perception test was conducted with 35 college students using /o/ and /u/ spoken by 41 females, which overlap considerably in the vowel space. An acoustic analysis of 182 stimuli was also conducted to see if there is any correspondence between production and perception. The identification rate was 89% on average, 86% for /o/, and 91% for /u/. The results confirmed that when /o/ and /u/ cannot be distinguished in the F1/F2 space because they are too close, H1-H2 differences contribute significantly to the separation of the two vowels. However, in perception, this was not the case. H1-H2 values were not significantly involved in the identification process, and the formants (especially F2) were still dominant cues. The study also showed that even though H1-H2 differences are apparent in females' production, males do not use H1-H2 in their production, and both females and males do not use H1-H2 in their perception. It is presumed that H1-H2 has not yet been developed as a perceptual cue for /o/ and /u/.

• Journal of Acupuncture Research
• /
• v.28 no.5
• /
• pp.121-126
• /
• 2011

Objectives : The aim of the study is to investigate the characteristics of Ryodoraku score accoding to Sasang constitution in the low back pain patients. Methods : For Roydoraku score examination, we made a comparison between 44 patients who were diagnosticated to low back pain(LBP group) and 35 persons who were not(non-LBP group). 1. We campared the variation from physiologic range of 12 Roydoraku points between LBP and non-LBP group. 2. We campared the variation from physiologic range of 12 Roydoraku points according to Sasang constitution between LBP and non-LBP group. 3. We campared the variation from physiologic range of 12 Roydoraku points according to Sasang constitution in LBP and non-LBP group. Results : 1. The score of H2 H5 F5 had statistical differences. 2. The score of H3 H5 F5 had statistical differences in Soeumin. 3. The score of H3 F4 had statistical differences in LBP gorup. Conclusions : These results suggest that roydoraku maybe used to evaluate low back pain patients.

• The Journal of the Korean dental association
• /
• v.13 no.1
• /
• pp.33-36
• /
• 1975

To determine the dental arch length and width in Korean yaung adults, various points (shown on Fig 1) were measured by means of Boley gauge on the 124 plaster models which obtained from 2 young men. The measurements value were as follows: A : 36.10㎜ a : 25.53㎜ B : 33.35㎜ b : 28.20㎜ C : 39.80㎜ c : 33.07㎜ D : 43.36㎜ d : 36.36㎜ E : 44.80㎜ e : 39.12㎜ F : 49.11㎜ f : 42.48㎜ G : 59.98㎜ g : 44.92㎜ H : 62.22㎜ h : 57.85㎜ I : 52.66㎜ i : 49.91㎜

• Journal of the Korean Chemical Society
• /
• v.38 no.5
• /
• pp.359-365
• /
• 1994

The crystal stucture of hexapotassium undecahydrogen tetratungsto hexaantimonate(V) tetrahydrate has been determined from single crystal X-ray diffraction data. Crystal data are as follows: $K_6H_{12}[Sb_6W_4O_{36}]{\cdot}4H_2O$, Fw = 2360.62, tetragonal, I$4_1$/a, a = 10.799(1) ${\AA}$, c = 35.244(5) ${\AA}$, V = 4110.1(7) ${\AA}^3$, Z = 4, $D_x$ = 3.82 g$cm^{-3}$, $\mu(MoK\alpha)$ = 160.15 $cm^{-1}$, T = 293 K, final R = 0.0356 for 2400($F_0 > 3\sigma(F_0))$ independent reflections. The $[H_{12}Sb_6W_4O_{36}]^{-6}$ polyanion independently consists of one tungsten, two antimony, and nine oxygen atoms and belongs to the $\bar4(S_4)$ point group. This polyanion is formed by two open octahedra five membered ring of Sb(3)$O_6-W(1)O_6-Sb(2)O_6-W(1)O_6-Sb(3)O_6$ which is connected at right angle. The Sb-W, Sb-O, and W-O bond distances range from 3.2304(9) to 3.2403(5) $\AA$, 1.745(8) to 2.334(6) $\AA$, and 1.914(7) to 2.039(7) $\AA$, respectively.

• 한국생물공학회:학술대회논문집
• /
• 2001.11a
• /
• pp.681-684
• /
• 2001

Pseudomonas sp. F721 isolated from soil produced a substance related in seeds germination inhibition. Addition of phytohormone, and GA (gibberellin acid) in the culture broth elevated production of the germination inhibition substance. The production of the substance was optimized in the culture conditions of $35^{\circ}C$, pH 9.0, 150 rpm, 48 hr, glucose 0.5% (w/v), and innoculation ratio 1.0% (v/v). The physical and chemical stability of the substance in the variety of pH ranging from 2.0 to 12.0 and from freezing to $100^{\circ}C$ were shown. The germination inhibition substance suppressed 90% of germination compared with that of the control experiment in a few days.

• Journal of the Institute of Electronics Engineers of Korea SD
• /
• v.42 no.9 s.339
• /
• pp.1-8
• /
• 2005

A $0.35\mu$m SiGe BiCMOS fabrication process has been timely developed, which is aiming at wireless RF ICs development and fast growing SiGe RF market. With non-selective SiGe epilayer, SiGe HBTs in this process used trapezoidal Ge base profile for the enhanced AC performance via Ge induced bandgap niuoin. The characteristics of hFE 100, $f_{T}\;45GHz,\;F_{max}\;50GHz,\;NF_{min}\;0.8dB$ have been obtained by optimizing not only SiGe base profile but also RTA condition after emitter polysilicon deposition, which enables the SiGe technology competition against the worldwide cutting edge SiGe BiCMOS technology. In addition, the process incorporates the CMOS logic, which is fully compatible with $0.35\mu$m pure logic technology. High Q passive elements are also provided for high precision analog circuit designs, and their quality factors of W(1pF) and inductor(2nH) are 80, 12.5, respectively.

• Tuberculosis and Respiratory Diseases
• /
• v.56 no.3
• /
• pp.280-288
• /
• 2004

Background : Lung protective strategies, using low tidal volume in ARDS, improve survival rate in ARDS. However, low tidal volume ventilation may promote alveolar de-recruitment. Therefore, alveolar recruitment is necessary to maintain arterial oxygenation and to prevent repetitive opening and closure of collapsed alveoli in lung protective strategies. There has been a recent report describing improvement in arterial oxygenation with use of recruitment maneuver. However, impact of recruitment on outcome of ARDS is unknown. We evaluated whether short-term survival difference existed in patients with ARDS, who were performed alveolar recruitment maneuver(ARM) and prone position, according to response of alveolar recruitment or not. Methods : All patients who were diagnosed with ADRS and received mechanical ventilation were included. ARM were sustained inflation($35-45cmH_2O$ CPAP for 30-40 sec.) or increasing level of PEEP. If these methods were ineffective, alveolar recruitment with prone position was done for at least 10 hours. $P_aO_2/FiO_2$(P/F) ratio was determined before and at 0.5 and 2 hours after ARM. We defined a responder if the P/F ratio was increased over 50% of baseline value. We compared 10-days and 30-days survival rate between responders and non-responders. Results : 20 patients(M:F=12:8, $63{\pm}14age$) were included. Among them, 12 patients were responders and 8 patients were non-responders. In responders, P/F ratio was increased from $92{\pm}25mmHg$ to $244{\pm}85mmHg$. In non-responders, P/F ratio increased from $138{\pm}37mmHg$ to $163{\pm}60mmHg$. Among non-responders, P/F ratio was improved over 50% in 2 patients after prone position. Overall, 14 patients were responders after ARM and prone position. The 10-days and 30-days survival rate in responders was significantly higher than in non-responders(86%, 57% in responders and 33%, 0% in non-responders)(p<0.05). There was no significant difference between responders and non-responders in age($71{\pm}11$, $60{\pm}14$), lung injury score($2.8{\pm}0.2$, $2.9{\pm}0.45$), simplified acute physiology score(SAPS) II ($35{\pm}4.6$, $34{\pm}5.7$), positive end-positive pressure level($15.6{\pm}1.9cmH_2O$, $14.5{\pm}2.1cmH_2O$). Conclusion : ARM may improve arterial oxygenation in some patients with ARDS. These responders in patients with ARDS showed significant higher 10-days and 30-days survival rate than non-responders patients with alveolar recruitment.