• Biomolecules & Therapeutics
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• v.20 no.6
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• pp.526-531
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• 2012

During our on-going studies to identify bioactive compounds in medicinal herbs, we found that saucerneol F (SF), a naturally occurring sesquilignan isolated from Saururus chinensis (S. chinensis), showed in vitro anti-inflammatory activity. In this study, we examined the effects of SF on the generation of 5-lipoxygenase (5-LO) dependent leukotriene $C_4$ ($LTC_4$), cyclooxygenase-2 (COX-2) dependent prostaglandin $D_2$ ($PGD_2$), and on phospholipase $C{\gamma}1$ ($PLC{\gamma}1$)-mediated degranulation in SCF-induced mouse bone marrow-derived mast cells (BMMCs). SF inhibited eicosanoid ($PGD_2$ and $LTC_4$) generation and degranulation dose-dependently. To identify the molecular mechanisms underlying the inhibition of eicosanoid generation and degranulation by SF, we examined the effects of SF on the phosphorylation of $PLC{\gamma}1$, intracellular $Ca^{2+}$ influx, the translocation of cytosolic phospholipase $A_2$ ($cPLA_2$) and 5-LO, and on the phosphorylation of MAP kinases (MAPKs). SF was found to reduce intracellular $Ca^{2+}$ influx by inhibiting $PLC{\gamma}1$ phosphorylation and suppressing the nuclear translocations of $cPLA_2$ and 5-LO via the phosphorylations of MAPKs, including extracellular signal-regulated protein kinase-1/2 (ERK1/2), c-Jun N-terminal kinase (JNK), and p38. Taken together, these results suggest that SF may be useful for regulating mast cell-mediated inflammatory responses by inhibiting degranulation and eicosanoid generation.

• Journal of the Korean Society of Tobacco Science
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• v.4 no.1
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• pp.21-27
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• 1982

Six flue-cured cultivars of Nicotiana tabacum L., 15 possible $F_1$ hybrids and 15 $F_2$ populations among them, and 15 haploid populations of $F_1$ hybrids and 6 haploid from parents, were evaluated. The variances of general combining ability were predominant for all characters studied in $F_1$ hybrid and $F_2$ population, and in haploid populations of $F_1$ hybrids except leaf width, value and reducing sugar. There were significant correlations between generations in variances and ranks of parental GCA effects estimated from general combining ability analysis of $F_1,\;F_2$ and haploid generation of $F_1$ hybrids for yield, stem height, days to flower. leaves per plant, value and total alkaloids.

• Archives of Pharmacal Research
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• v.29 no.6
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• pp.469-475
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• 2006

A series of yakuchinone B 1f and its analogs 1a-e was synthesized and evaluated for free radical scavenging, suppression of LPS-induced NO generation, cytotoxicity and anti-excitotoxicity in vitro. Compound 1c exhibited potent anti-excitotoxicity, while all compounds 1a-f showed considerable effects of free radical scavenging, suppression of LPS-induced NO generation, and cytotoxicity in microglia.

• Journal of Embryo Transfer
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• v.27 no.1
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• pp.9-14
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• 2012

This study was conducted to analyze the transgenic efficiency and sex ratio in ${\alpha}$-1,3-galactosyltransferase (GalT) knock-out (KO) transgenic pigs according to generation. GalT KO piglets were produced by artificial insemination or natural mating. The transgenic confirmation of GalT KO was evaluated by PCR amplification using specific primers. After electrophoresis, three types of bands were detected such as 2.3 kb single band (Wild), 2.3 and 3.6kb double bands (GalT KO -/+; heterozygote), and 3.6kb single band (GalT KO -/-; homozygote). Transgenic efficiency in F1 generation was 64.5% (23/35) of GalT KO (-/+). In F2 generation, GalT KO transgenic efficiency was 36.4% (21/57, Wild), 47.5% (28/57, GalT KO -/+), and 16.1% (8/57, GalT KO -/-), respectively. Interestingly, no homozygote piglets were born in 6 deliveries among total 11 deliveries, although they were pregnant between male (M) and female (F) $F_1$ heterozygote. In the 5 litters including at least one GalT KO -/- piglet, the transgenic efficiency was 13.3% (2/24, Wild), 51.3% (14/24, GalT KO -/+), and 35.3% (8/24, GalT KO -/-), respectively. The sex ratio of M and F was 40:60 in $F_1$ and 49:51 in $F_2$ generation, respectively. Based on these results, GalT KO transgenic pigs have had a reproductive ability with a normal range of transgenic efficiency and sex ratio.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.85-85
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• 2003

Human lactoferrin (hLF) is an 80 kDa iron-binding glycoprotein that is expressed in high concentration in milk and in lesser amount in the secondary or specific granules of neutrophils and in plasma, LF is classically considered to be related to the binding, transport, and storage of iron. The transgenic mice carrying the human hLF gene in conjunction with the bovine $\beta$-casein promoter produced the human hLF in their milk during lactation. To screen transgenic mice, PCR was carried out using chromosomal DNA extracted from tail or toe tissues. In this study, stability of germ line transmission and expression of hLF were monitored up to generation Fl7 of a transgenic line. When female mouse of generation F9 was crossbred with normal male, generation F9 to Fl7 mice showed similar transmission rates ($66.0 \pm 12.57%, 42.0 \pm 14.98%, 72.2 \pm 25.45%, 50.0 \pm 16.70%, 65.7 \pm 6.45%, 48.6 \pm 14.65%, 54 1 \pm 18 11%, 57.8 \pm 16.16% and 48.6 \pm 20.66$, respectively), implying that the hLF gene can be transmitted stably up to long term generation in the transgenic mice For ELISA analysis, hLF expression levels were determined with an hLF ELISA kit in accordance with the supplier's protocol. Expression levels of human hLF from milk of generation F9 to Fl3 mice were $ 3.2 \pm 0.69 mg/ml, 3.1 \pm 0.81 mg/ml, 4.6 \pm 1.38 mg/ml, 3.1 \pm 0.42 mg/ml, and 4.5 \pm 1,48 mg/ml$, respectively. These expression levels were lower than that of founder (6.6 mg/$m\ell$) mouse. We concluded that transgenic mice faithfully passed the transgene on their progeny and successively secreted target proteins into their milk through several generations.

• Journal of the Ergonomics Society of Korea
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• v.25 no.4
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• pp.9-22
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• 2006

1/f fluctuation characteristics can be seen in various natural phenomena, such as breezes, streams, candle flames and the luminous patterns of fireflies. It is said that the 1/f fluctuation are comfortable for human beings. And they are introduced into many industrial products, such as an air conditioner, music, lighting, etc. This study focused on verifying the effects of 1/f fluctuation sound on comfort sensibility. Stimulus were divided into three groups(Group 1, 2, 3) by sound generation methodology. Fluctuation patterns of group 1, 2 were given by three types of fluctuation, 1/f0, 1/f1, 1/f2, and its of group 3 were given by two types of pure tone, 1/f1. In order to verify the effects, we measured the physiological responses of the subjects such as EEG(Electroencephalogram), ERP(Event-Related Potential), and these physiological responses were compared with subjective assessments, free answers. Consequently, we found that factor which had an effect on comfort sensibility was cognitive factor(for stimulus) rather than 1/f fluctuation sound pattern.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.42 no.6
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• pp.703-710
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• 2009

The spectral irradiance and underwater transmission characteristics of a combined high-luminance light-emitting diode (LED) lights have been studied to evaluate suitable light sources for fishing lamps of the next generation. The wavelengths at which the irradiance was maximum were changed from 473, 501, 525, and 465 nm for blue, peacock blue, green, and white LED light to 475, 504 and 528 nm for [$F_{WB}$], [$F_{PB}$] and [$F_{GB}$] combined LED lights, respectively. If the irradiance characteristics at 400-700 nm wavelengths are set as 100%, the irradiance rates at 450-499 nm and 500-549 nm were decreased from 82.4% and 56% for blue, peacock blue LED light to 60.0%, 38.5% for [$F_{WB}$], [$F_{WP}$] combined LED lights. The underwater transmission characteristics of the combined LED lights were superior in the order [$F_{WB}$], [$F_{BP}$], [$F_{GB}$] in optical water type I; [$F_{WB}$], [$F_{PB}$], [$F_{GP}$] in optical water type II-III; and [$F_{GP}$], [$F_{WP}$], [$F_{PB}$] in optical water type 1. Setting the 10m depth underwater transmission characteristics of the combined LED lights in optical water type I at 100%, the transmission of water types II, III and 1 drops to 29.5%, 8.0% and 2.2%. Based on the distribution of spectral irradiance and underwater transmission characteristics calculated in optical water types II-III, where was the jigging ground for fishing lamps, the [$F_{WB}]$ and [$F_{GP}$] combined LED lights can be used as a suitable light sources for fishing lamps of the next generation.

• Bulletin of the Korean Chemical Society
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• v.32 no.1
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• pp.183-185
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• 2011

Adenylate kinase-like activity and phosphotransferase-like activity from $F_1$-ATPase of Escherichia coli was revealed by $^{31}P$ NMR spectroscopy. Incubation of F1-ATPase with ADP in the presence of $Mg^{2+}$ shows the appearance of $^{31}P$ resonances from AMP and Pi, suggesting generation of AMP and ATP by adenylate kinase-like activity and the subsequent hydrolysis to Pi. Incubation of $F_1$-ATPase with ADP in the presence of methanol shows additional peak from methyl phosphate, suggesting phosphotransferase-like activity of $F_1$-ATPase. Both adenylate kinase-like activity and phosphotransferase-like activity has not been reported from $F_1$-ATPase of Escherichia coli. $^{31}P$ NMR could be a valuable tool for the investigation of phosphorous related enzyme.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.4
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• pp.386-391
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• 1988

Five barley crosses and their progenies (F$_2$, F$_3$, and F$_4$) were evaluated the potentiality of hybrid populations to segregate superior yielders in later generation. Four characters used for evaluation were number of spike, number of grain, spike weight and grain weight per plant. Superiority value (Y) of number of spike was best in SB76588${\times}$SB72648 and average of superior plants in F$_4$, was excellent in this cross. Milyang 6${\times}$Suwon 203 showed high Y value and average of F$_4$ superior plants in the number of grain. Spike weight showed the highest Y value and excellent averages of superior plant in Milyang 6${\times}$Suwon 203. Superiority value of grain weight per plant coincided with average of F$_4$ superior plants in 3 crosses and SB76588${\times}$SB72648 was the best among the crosses.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.6
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• pp.896-904
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• 2013

Cattle breeds have a striking effect on milk, including milk composition and technological characteristics. This study aims to compare milk composition, acidification activity, viscosity, milk dispersion system stability and casein molecular weight among three buffalo breeds in China. The technological characteristics of milk produced by three cattle breeds of river buffalo (Murrah), crossbreed 1st generation ($F_1$), crossbreed multiple generation ($F_H$, $H{\geq}3$) buffaloes were investigated. Cattle breeds showed evident effect on milk protein, fat and total solids content, but little effect on most of buffalo casein molecular weight. Milk fat, protein content and the viscosity of buffalo milk from river buffalo were lower than those of $F_1$ and $F_H$, so was the buffer capacity. The viscosity was negatively correlated to temperature and concentration. Results of stability coefficient showed that milk dispersion system had the best dynamic stability characteristics under pH 6.6 and 6 times dilution, while zeta potential of Murrah milk was slightly higher than that of hybrid offspring ($F_1$, $F_H$). SDS-PAGE results showed that buffalo ${\alpha}_s$-casein had a slightly faster mobility than standard ${\alpha}_s$-casein; while buffalo ${\beta}$-casein showed a slightly slower mobility than standard ${\beta}$-casein. There is no clear differences in molecular weight of ${\alpha}_s$-, ${\beta}$-, and ${\kappa}$-casein among Murrah, $F_1$ and $F_H$.