• Journal of Periodontal and Implant Science
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• 제32권4호
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• pp.733-744
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• 2002

The fibroblasts are the principal cells in the periodontal ligament of peridontium. As the periodontal ligament fibroblasts (PDLF) show similar phenotype with osteoblasts, the PDLF are thought to play an important role in alveolar bone remodeling. Cell-to-cell contacted signaling is crucial for osteoclast formation. Recently it has been reported that PDLJ enhance the bone resorbing activity of osteoclasts differentiated from hematopoietic preosteoclasts. The aims of this study were to $clarify\;^{1)}$ the mechanism of PDLF-induced osteoclastogenesis $and\;^{2)}$ whether we can use preosteoclast cell line instead of primary hematopoietic preosteoclast cells for studying the mechanism of PDLF-induced osteoclastogenesis. Osteoclastic differentiation of mouse macrophage cell line RAW264.7 was compared with that of mouse bone marrow-derived M-CSF dependent cell (MDBM), a well-known hematopoietic preosteoclast model, by examining, 1) osteoclast-specific gene expression such as calcitonin receptor, M-CSF receptor (c-fms), cathepsin K, receptoractivator nuclear factor kappa B (RANK) ,2) generation of TRAP(+) multinucleated cells (MNCs), and 3) generation of resorption pit on the $OAAS^{TM}$ plate. RAW264.7 cultured in the medium containing of soluble osteoclast differentiation Factor (sODF) showed similar phenotype with MDBM-derived osteoclasts, those are mRNA expression pattern of osteoclast-specific genes, TRAP(+) MNCs generation, and bone resorbing abivity. Formation of resorption pits by osteoclastic MNCs differentiated from sODF-treated RAW264.7, was completely blocked by the addition of osteoprotegerin (OPG), a soluble decoy receptor for ODF, to the sODF-containing culture me야um. The effects of PDLF on differentiation of RAW264.7 into　the TRAP(+) multinucleated osteoclast-like cells were examined using coculture system. PDLF were fxed with paraformaldehyde, followed by coculture with RAW264.7, which induced formation of TRAP(+) MNCs in the absence of additional treatment of sODF. When compared with untreated and fixed PDLF (fPDLF), IL-1 ${\beta}$-treated, or lipopolysaccha-ride-treated and then fixed PDLF showed two-folld increase in the supporting activity of osteoclastogenesis from RAW264.7 coculture system. There were no TRAP(+) MNCs formation in coculture system of RAW264.7 with PDLF of no fixation. These findigs suggested that we can replace the primary hematopoietic preosteoclasts for RAW264. 7 cell line for studying the mechanism of PDLF-induced osteoclastogenesis, and we hypothesize that PDLF control osteoclastogenesis through ODF expression which might be enhanced by inflammatory signals.

• 한국조리학회지
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• 제21권1호
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• pp.250-266
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• 2015

오늘날 실버세대는 독립된 소비세대로 사회 여러 분야에서 실버외식산업을 급성장시키는 세대이기도 하다. 본 연구에서는 새로운 소비의 주체세력으로 부상하고 있는 실버세대를 대상으로 실버세대에 대한 외식 소비행동에 따른 외식 선택속성과 식습관 변화를 살펴보고자 하였다. 서울에 거주하는 55세 이상의 실버 연령인 216명을 대상으로 하여 식습관 수준과 인구사회학적 특성에 따른 변인분석은 독립표본 t-검증과 F-검증을 실시하였으며, 실버세대의 식습관에 따른 소비태도와 외식소비행동 차이를 알아보기 위하여는 ${\chi}^2$검증을 실시하였다. 분석한 연구 결과를 요약하여 다음과 같은 결과를 얻었다. 첫째, 실버세대의 식습관 수준은 3점 만점에 평균 2.02점으로 나타나, 식습관이 어느 정도 양호함을 알 수 있었다. 둘째, 실버세대의 식습관에 따른 외식소비태도 차이를 분석한 결과, 식습관 태도가 비교적 양호한 사람들이 불량한 집단보다 물리적인 환경, 메뉴의 다양성, 업체의 품질과 서비스를 찾아서 외식업체를 선택하는 것으로 나타났다. 셋째, 실버세대의 식습관에 따른 외식소비행동 변화를 분석한 결과, 식습관 수준에 따른 외식업체 정보 출처와 외식업체 선택 선정기준 변수에 대해서만 집단 간 차이가 있는 것으로 나타났다.

• Corrosion Science and Technology
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• 제4권3호
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• pp.108-113
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• 2005

One of the recent safety issues in the pressurized heavy water reactor (PHWR) is the cracking of the feeder pipe. Because of the limited accessibility to the cracked region and a high dose of radiation exposure, it is difficult to inspect all the pipes with the conventional ultrasonic method. In order to solve this problem, a long-range guided wave technique has been developed. A computer program to calculate the dispersion curves in the pipe was developed and the dispersion curves for the feeder pipes in PHWR plants were determined. Several longitudinal and/or flexural modes were selected from the review of the dispersion curves and an actual experiment has been carried out with the specific alignment of the piezoelectric ultrasonic transducers. They were confirmed as L(0,1)) and/or flexural modes(F(m,2)) by the short time Fourier transformation(STFT) and were sensitive to the circumferential cracks, but not to the axial cracks in the pipe. An electromagnetic acoustic transducers(EMAT) was designed and fabricated for the generation and reception of the torsional guided wave. The axial cracks were detected by a torsional mode(T(0,1)) generated by the EMAT.

• 한국광학회:학술대회논문집
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• 한국광학회 2000년도 제11회 정기총회 및 00년 동계학술발표회 논문집
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• pp.284-285
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• 2000

광굴절률 현상은 optical signal processing과 홀로그램 기억소자로 널리 응용 될 수 있기 때문에 지금까지 광범위하게 연구되어져 왔다. 광굴절률 현상에서 중요한 변수는 빛이 있는 동안 drift, diffusion 과 photovoltaic current와 같은 전하 운반 메카니즘을 통해서 local charge의 재분포에 따른 공간전하장(Space-charge field, $E_{sc}$ )이다. 지금까지 single beam에 의한 공간전하장을 측정하는 방법으로 birefringenc $e^{1.2}$ 와 interference metho $d^{3}$을 이용하여 굴절률 변화를 측정함으로써 얻을 수 있었다. 그러나 이런 방법들은 공간전하장의 변화를 측정하기위해서 전기광학계수를 측정하여 얻는 간접적인 방법이고 또한 실험방법도 다소 복잡하다. 따라서 본 투고에서는 이미 발표된 광굴절률 현상시 제 2 고조파 세기(SHG)의 변화로부터 공간전하장을 간단하게 측정하는 방법을 이용하여 congruent, MgO가 4mole%, F $e_2$O가 0.1mole% 첨가된 LiNb $O_3$ 단결정의 공간전하장에 대해서 연구를 하였다. 이 방법은 전기광학물질인 LiNb $O_3$에서 SHG 위상정합조건이 dc 전기장에 의존하는 성질을 이용한 것이다. 그리고 온도가 일정할 경우 전기장의 변화에 따라 SHG의 크기가 변함을 이용하였다. (중략)

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제10권7호
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• pp.3353-3370
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• 2016

The way of combination of Product Perfect Codes (PPCs) is based on the theory of short codes constructing long codes. PPCs have larger embedding rate than Hamming codes by expending embedding columns in a coding block, and they have been proven to enhance the performance of the F₅ steganographic method. In this paper, the proposed modified product codes called MPCs are introduced as an efficient way to embed more data than PPCs by increasing 2^r₂-1-r₂ embedding columns. Unlike PPC, the generation of the check matrix H in MPC is random, and it is different from PPC. In addition a simple solving way of the linear algebraic equations is applied to figure out the problem of expending embedding columns or compensating cases. Furthermore, the MPCs over ℤ₄ have been proposed to further enhance not only the performance but also the computation speed which reaches O(n₁+σ). Finally, the proposed ℤ₄-MPC intends to maximize the embedding rate with maintaining less distortion , and the performance surpasses the existing improved product perfect codes. The performance of large embedding rate should have the significance in the high-capacity of covert communication.

• 로봇학회논문지
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• 제13권1호
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• pp.16-25
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• 2018

The talking head (TH) indicates an utterance face animation generated based on text and voice input. In this paper, we propose the generation method of TH with facial expression and intonation by speech input only. The problem of generating TH from speech can be regarded as a regression problem from the acoustic feature sequence to the facial code sequence which is a low dimensional vector representation that can efficiently encode and decode a face image. This regression was modeled by bidirectional RNN and trained by using SAVEE database of the front utterance face animation database as training data. The proposed method is able to generate TH with facial expression and intonation TH by using acoustic features such as MFCC, dynamic elements of MFCC, energy, and F0. According to the experiments, the configuration of the BLSTM layer of the first and second layers of bidirectional RNN was able to predict the face code best. For the evaluation, a questionnaire survey was conducted for 62 persons who watched TH animations, generated by the proposed method and the previous method. As a result, 77% of the respondents answered that the proposed method generated TH, which matches well with the speech.

• Fisheries and Aquatic Sciences
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• 제15권1호
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• pp.43-47
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• 2012

Growth hormone (GH) transgenesis in fish has the potential to improve aquaculture efficiency and capacity. However, many fast-growing transgenic fish have experienced side effects caused by excess GH expression. To overcome this unwanted issue associated with several GH transgenic mud loach Misgurnus mizolepis lines carrying GH construct driven by a strong ${\beta}$-actin regulator ($pml{\beta}$-actGH), we performed an alternative version of GH autotransgenesis using a weaker but more stable regulator, the mud loach lectin promoter. GH transgenesis with a pmlectGH construct consisting of the mud loach GH gene driven by the 2.3-kb lectin promoter exhibited significant growth stimulation. However, the extent of the growth acceleration in pmlectGH transgenics (six times maximum when assessed 2 months post hatching) was much less than that in transgenic individuals carrying the $pml{\beta}$-actGH construct. Additionally, the extraordinary gigantism that was common in $pml{\beta}$-actGH-transgenic mud loaches was diminished in transgenic loaches harboring the pmlectGH construct. Transgenic founders (pmlectGH) successfully transmitted their transgene into the next generation with up to 41% frequency. Growth stimulation also persisted in the transgenic F1 strains, with a seven-fold increase in maximum body weight at 6 months of age.

• 한국군사과학기술학회지
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• 제16권1호
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• pp.27-33
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• 2013

Helmet Mounted Display(HMD) has great advantages on the navigation and mission symbologies for the pilot's forward looking display and, therefore, has been remarkably drawing attention as the up coming display of the next generation aircraft. The essential technology to process the Line of Sight-Foward(LOS-F) data in real-time is to estimate exact helmet situation and position. In this paper, we research a acoustic helmet tracking technique. For the reason that mechanical acoustic noises might interfere with Helmet Tracking System(HTS) and unnecessary acoustic noises are inevitable when using acoustic technique, this approach has not been adapted. In order to overcome this problem. We propose that acoustic wave of which the wave length is longer than audio frequency and, especially, we used beating signal envelope which is composed of two close high frequency.

• Journal of Power Electronics
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• 제19권1호
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• pp.234-243
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• 2019

Studying the control strategy of a microgrid under the load unbalanced state helps to improve the stability of the system. The magnitude of the power fluctuation, which occurs between the power supply and the load, is generated in a microgrid under the load unbalanced state is called negative sequence reactive power $Q^-$. Traditional power distribution methods such as P-f, Q-E droop control can only distribute power with positive sequence current information. However, they have no effect on $Q^-$ with negative sequence current information. In this paper, a stationary-frame control method for power sharing and voltage unbalance compensation in islanded microgrids is proposed. This method is based on the proper output impedance control of distributed generation unit (DG unit) interface converters. The control system of a DG unit mainly consists of an active-power-frequency and reactive-power-voltage droop controller, an output impedance controller, and voltage and current controllers. The proposed method allows for the sharing of imbalance current among the DG unit and it can compensate voltage unbalance at the same time. The design approach of the control system is discussed in detail. Simulation and experimental results are presented. These results demonstrate that the proposed method is effective in the compensation of voltage unbalance and the power distribution.

• Genomics & Informatics
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• 제19권1호
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• pp.7.1-7.13
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• 2021

Levodopa (L-DOPA) therapy is normally practised to treat motor pattern associated with Parkinson disease (PD). Additionally, several inhibitory drugs such as Entacapone and Opicapone are also cosupplemented to protect peripheral inactivation of exogenous L-DOPA (~80%) that occurs due to metabolic activity of the enzyme catechol-O-methyltransferase (COMT). Although, both Entacapone and Opicapone have U.S. Food and Drug Administration approval but regular use of these drugs is associated with high risk of side effects. Thus, authors have focused on in silico discovery of phytochemicals and evaluation of their effectiveness against human soluble COMT using virtual screening, molecular docking, drug-like property prediction, generation of pharmacophoric property, and molecular dynamics simulation. Overall, study proposed, nine phytochemicals (withaphysalin D, withaphysalin N, withaferin A, withacnistin, withaphysalin C, withaphysalin O, withanolide B, withasomnine, and withaphysalin F) of plant Withania somnifera have strong binding efficiency against human COMT in comparison to both of the drugs i.e., Opicapone and Entacapone, thus may be used as putative bioenhancer in L-DOPA therapy. The present study needs further experimental validation to be used as an adjuvant in PD treatment.