• Proceedings of the Korea Concrete Institute Conference
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• 1992.04a
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• pp.137-142
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• 1992

The purpose of this study is to develop a new structural system for precast deck plate bridge. In order to investigate load distribution and composite effects by connecting diaphragms and precast deck plate to main girder with shear keys, computer analysis with F.E.M S/W package program ADINA was carried out. As results of analysis, the following could be concluded : -The distance between diaphragms with6m is suficient for crossing load distribution effects. -The composite effects by connection deck plate to main girder with shear key is reasonable. -The gap between deck plate and main girder under given load is negligible.

• Journal of Life Science
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• v.15 no.3 s.70
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• pp.415-422
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• 2005

library of the mutants was prepared by transposon mutagenesis of the Mycobacterium bovis BCG. We screened this library for the resistance to an anti-tuberculosis antibiotic, PA-824. Most of the mutants resistant to the PA-824 were not able to synthesize the coenzyme $F_{420}$ which is normally produced by the wild type M. bovis BCG strains. HPLC analysis of the cellular extract showed that one of those mutants which lost the ability to synthesize $F_{420}$ still produced F0. The insertion site of the transposon in this mutant was determined by an inverse PCR and the transposon was found to be inserted in the Rv2435c open reading frame (ORF). Rv2435c ORF is predicted to encode an 80.3 kDa protein. Rv2435c protein appears to be bound to the cytoplasmic membrane, its N-terminal present in the periplasm and C-terminal in the cytoplasm. The C-terminal portion of this protein is highly homologous with the adenylyl cyclases of both prokaryotes and eukaryotes. There are 15 ORFs which have homology with the class III AC proteins in the genome of the M. tuberculosis and M. bovis. Two of those, Rv1625c and Rv2435c, are highly homologous with the mammalian ACs. We cloned the cytoplasmic domain of the Rv2435c ORF and expressed it with six histidine residues attached on its C-terminal in Escherichia coli to find out if this protein is a genuine AC. Production of that protein in E. coli was proved by purifying the histidine-tagged protein by using the Ni-NTA resin. This protein, however, failed to complement the cya mutation in E. coli, indicating that this protein lacks the AC activity. All of the further attempts to convert this protein to a functional AC by a mutagenesis with UV or hydroxylamine, or construction of several different fusion proteins with Rv1625c failed. It is, therefore, possible that Rv2435c protein might affect the conversion of F0 to $F_{420}$ not by synthesizing cAMP but by some other way.

• Journal of Microbiology and Biotechnology
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• v.18 no.4
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• pp.648-657
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• 2008

In this study, we used functional genomic strategies, proteomics and quantitative real-time (qRT)-PCR, to advance our understanding of genes associated with fumonisin production in the fungus Fusarium verticillioides. Earlier studies have demonstrated that deletion of the FCC1 gene, which encodes a C-type cyclin, leads to a drastic reduction in fumonisin production and conidiation in the mutant strain (FT536). The premise of our research was that comparative analysis of F. verticillioides wild-type and FT536 proteomes will reveal putative proteins, and ultimately corresponding genes, that are important for fumonisin biosynthesis. We isolated proteins that were significantly upregulated in either the wild type or FT536 via two-dimensional polyacrylamide gel electrophoresis, and subsequently obtained sequences by mass spectrometry. Homologs of identified proteins, e.g., carboxypeptidase, laccase, and nitrogen metabolite repression protein, are known to have functions involved in fungal secondary metabolism and development. We also identified gene sequences corresponding to the selected proteins and investigated their transcriptional profiles via quantitative real-time (qRT)-PCR in order to identify genes that show concomitant expression patterns during fumonisin biosynthesis. These genes can be selected as targets for functional analysis to further verify their roles in $FB_1$ biosynthesis.

• Journal of Korean Society of Forest Science
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• v.107 no.4
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• pp.385-392
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• 2018

The slope stabilization analysis was performed by conducting survey and selecting the representative section in order to improve slope composition and management technology of masonry embankments in the quarry area, The mean slope of the masonry retain wall (A, B, C, D, E, F) was $38.5^{\circ}$, although the steep slope of the lowest slope (A) as $59^{\circ}$. The horizontal distance of the masonry embankments is 66.2 m and the slope height is 48.3 m. However, the inclination of the masonry embankments is relatively steep and visually unstable. The slope stability analysis for the slope stability analysis was taken into account during the drying and saturation. The slope stability analysis during saturation was performed by modeling the fully saturated slope. The strength constants of the ground were divided into two groups. The safety factor for dry period was 1.850 and the safety factor for rainy season was 1.333. The safety rate of dry period and rainy season was above 1.5 and 1.2. However, the weathered granite on the upper part of the masonry embankments at the time of heavy rainfall is considered to have a high risk of slope erosion and collapse. Therefore, it is considered necessary to take measures for stabilization through appropriate maintenance such as drainage installation.

• Journal of the Korean Society of Safety
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• v.28 no.2
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• pp.26-30
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• 2013

The purpose of this study is to find risk factors by analyzing the operation principle of a photo coupler (P/C) used to remove the noise of electronic devices and establish a base for the performance improvement of developed products. It was found from the P/C circuit analysis of normal products that they were equipped with an electrolytic condenser of $0.1{\mu}F$ to smooth system signals. Due to the epoxy resin packing the external part of the P/C, this study experienced a limit to visually examine the damage to it. It could be seen from the analysis of electric characteristics of the P/C that the forward voltage ($V_f$) and reverse current ($I_r$) were 1.3 V and 10 uA, respectively. In addition, it is required that the breakdown voltage (VCE) between the collector (C) and emitter (E) be maintained at less than 35 V. The and of the damaged product #1 were comparatively good. However, the measurement of was 100.0 uA. From this, it is thought that a short circuit occurred to the internal circuit. Moreover, from the fact that the of the damaged product #2 was open circuit and the measurement of was 0.0 uA, it is thought that the collector and emitter was separated or insulation resistance was significantly high. Furthermore, from the fact that the of the damaged product #3 was open circuit and the measurement of was 0.0 uA, it is thought that the space between the collector (C) and emitter (E) failed to meet the design standard or that they were separated. Therefore, it is thought that fabricating the P/C by increasing the reverse current 10 mA to 50 mA will prevent its malfunction.

• Journal of Welding and Joining
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• v.17 no.1
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• pp.88-96
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• 1999

The effects of root gap on welding residual stress and deformation are dealt with the multi-pass weldment with three kinds(0, 6, 30mm) of root gap by F.E.M common code, and then compared with experiment data. In this analysis, an 100% ramp heat input model was used to avoid numerical convergence problem due to an instantaneous increase in temperature near the fusion zone, and the effect of a moving arc in a two dimensional plane was also included. During the analysis, a small time increment was applied in a period with instantaneous temperature fluctuation while a large time increment was used in the rest period. The residual stress is distributed as symmetric types and maximum value is also equivalent when the weldment with 0mm and 6mm root gap is welded. In the case of 30mm root gap welding, the distribution of the residual stress extends over a wide range as asymmetric types due to the built-up weld, and most of the residual stress is biased in the side of a built-up weld part. In case of 0mm gap welding and 6mm gap welding, a little angular distortion occurs, but the level of deformation is small. When the weldment with 30mm root gap is welded, the angular deformation of the asymmetric types, however, occurs larger than the other specimens. The experimental and the analytic results show good coincidence and indicate that the welding residual stress and deformation distribution of 30 mm root gap specimen may be asymmetric and the amplitude is larger than those of root gap specimen under standard.

• Journal of the Korean Geotechnical Society
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• v.24 no.11
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• pp.157-166
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• 2008

To investigate the relationship between compressive strength ($q_u$) of Lightweight Air-mixed soil (LAS) and its physical deformation coefficient ($E_{50}$), a series of unconfined compressive tests have been performed on specimens of LAS according to various dredged soil types by percentage of sand, silt and clay. From the results it was found that the cement content ($C_i$) and unit weight (${\gamma}_m$) are most influence factors on strength, and percentage of sand, silt, clay by grain size analysis (KS F2302) have more effect on compressive strength than other physical properties of soil. It was also found that the rate of strength (a) increases with curing time, but it reduces with the increase of percentage of clay ($C_%$).

• Molecules and Cells
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• v.25 no.3
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• pp.417-427
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• 2008

Comparison of maps and QTLs between populations may provide us with a better understanding of molecular maps and the inheritance of traits. We developed and used two reciprocal $BC_1F_1$ populations, IP/DS//IP and IP/DS//DS, for QTL analysis. DS (Dasanbyeo) is a Korean tongil-type cultivar (derived from an indica x japonica cross and similar to indica in its genetic make-up) and IP (Ilpumbyeo) is a Korean japonica cultivar. We constructed two molecular linkage maps corresponding to each backcross population using 196 markers for each map. The length of each chromosome was longer in the IP/DS//IP population than in the IP/DS//DS population, indicating that more recombinants were produced in the IP/DS//IP population. Distorted segregation was observed for 44 and 19 marker loci for the IP/DS//IP and IP/DS//DS populations, respectively; these were mostly skewed in favor of the indica alleles. A total of 36 main effect QTLs (M-QTLs) and 15 digenic epistatic interactions (E-QTLs) were detected for the seven traits investigated. The phenotypic variation explained (PVE) by M-QTLs ranged from 3.4% to 88.2%. Total PVE of the M-QTLs for each trait was significantly higher than that of the E-QTLs. The total number of M-QTLs identified in the IP/DS//IP population was higher than in the IP/DS//DS population. However, the total PVE by the M-QTLs and E-QTLs together for each trait was similar in the two populations, suggesting that the two $BC_1F_1$ populations are equally useful for QTL analysis. Maps and QTLs in the two populations were compared. Eleven new QTLs were identified for SN, SF, GL, and GW in this study, and they will be valuable in marker-assisted selection, particularly for improving grain traits in tongil-type varieties.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.15 no.4
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• pp.332-337
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• 2002

The applicability of models based on fractal geometry to characterize the surface of the EL devices was investigated. Insulating layer and phosphor layer of EL devices were deposited on ITO glass using e-beam method. The images of phosphor layer by scanning electron microscope(SEM) were transformed to binary coded data. The relations between fractal geometry and electrical characteristics of EL devices were investigated. When the fractal dimension of $Cas:EuF_3$ EL device was 1.82 and its grain boundary area was 19%, the brightness of $Cas:EuF_3$ EL device was 261 cd/$\textrm{m}^2$.

• Journal of the Korean institute of surface engineering
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• v.39 no.1
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• pp.35-42
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• 2006

In this study, the fatigue properties on the cavitation damage of the flame quenched 8.8Al-bronze (8.8Al-4.5Ni-4.5Fe-Cu) as well as the current nuclear pump impeller materials (8.8Al-bronze, STS316 and SR50A) has been investigated using an ultrasonic vibratory cavitation test. For this the impact loads of cavitation bubbles generated by ultrasonic vibratory device quantitatively evaluated and simultaneously the cavitation erosion experiments have been carried out. The fatigue analysis on the cavitation damage of the materials has been made from the determined impact load distribution (e.g. impact load, bubble count) and erosion parameters (e.g. incubation period, MDPR). According to Miner's law, the determined exponents b of the F-N relation ($F^b$ N = Constant) at the incubation stage (N: the number of fracture cycle) were 5.62, 4.16, 6.25 and 8.1 for the 8.8Al-bronze, flame quenched one, STS316 and SR50A alloys. respectively. At the steady state period, the exponents b of the F-N' curve (N': the number of cycle required for $1{\mu}m$ increment of MDP) were determined as 6.32, 5, 7.14 and 7.76 for the 8.8Al-bronze, flame quenched one, STS316, and SR50A alloys, respectively.