• 한국수정란이식학회지
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• 제11권3호
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• pp.201-210
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• 1996

This experiment was carried out to evaluate the effect of early mouse embryonic development in vitro by co-culture with bovine and porcine oviductal epithelial cells (BOEC and POEC). The 2-cell embryos were collected from the oviducts of the superovulated and mated cultured in D-PBS /15% FCS at 48 hours after hCG injection. The in vitro developmental rate of blastocyst formation in the embryos were examined under the fllowing treatments; 1) TCM 199 added 15% HCS, 2) Ham's F-10 added 15% HCS, 3) MediCult IVF medium, 4) TCM 199 added 15% HCS + BOEC, 5) TCM 199 added 15% HCS + POEC, 6) Ham's F40 added 15% HCS + BOEC, 7) Ham's F-10 added 15% HCS + POEC,8) MediCult IVF medium + BOEC, 9) MediCult IVF medium + POEC. For a comparative study of in vitro development for 96 hours after hCG injection, were cultured with oviductal epithelial cell and media only. The obtained results were 2-cell embryos developed to the blastocyst stage in TCM 199, Ham's F-10 and MediCult IVF medium at the rates of 84.4,83.2 and 81.6%. respectively. The higher developmental rates(91~97%) of blastocyst formation was appeared when the embryos were co-cultured with a monolayer of bovine or porcine oviductal epithelial cells in TCM 199 or Ham's F-10 and MediCult IVF media. No significant difference in developmental rates was shown between bovine and porcine oviductal epithelial cells but significant difference in co-culture system in comparison between media only system and co-cultures. In conclusions, oviductal epithelial cells, BOEC and POEC, when co-culture with mouse early embryos improved the rates of development, blastocyst and hatching. Therefore, it is suggested that co-culture system using oviductal epithelial cells improve early embryonic developtnent in mouse.

• 한국유기농업학회지
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• 제31권4호
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• pp.427-439
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• 2023

The silverleaf whitefly, Bemisia tabaci, is a major pest distributing worldwide damaging over 900 host plant species, and is highly resistant to chemical pesti- cides. Due to the high pesticide resistance of whitefly, there is a need for alternatives to chemical control. Entomopathogenic fungi are candidates for biological pesticide that can overcome the resistance problem of chemical pesticide. Therefore, in this study, we tested pathogenicity of the entomopathogenic fungi to select high insec- ticidal activity against whitefly. As a result, IPBL-C (Cordyceps fumosorosea) and IPBL-F (Metarhizium pinghaense) isolates showed high insecticidal activity against whitefly. Additionally, as a result of culturing the selected isolates on spent coffee grounds medium, the conidia of IPBL-F produced on coffee grounds medium showed five times higher heat stability after heat treatment at 45℃ for one hour than conidia produced on PDA medium.

• International Journal of Oral Biology
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• 제39권1호
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• pp.35-40
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• 2014

Halitosis is caused by consumption of certain foods or drinks and production of volatile sulfur compounds (VSCs) by periodontopathogens. VSCs-related halitosis is not easily removed using mechanical or chemical therapies such as dental floss, plaque control and mouth rinse. Lactobacillus are known to be probiotics and stimulate immune systems of human. Furthermore, L. casei ATCC 334 and L. rhamnosus GG have an effect on protection of dental caries in vitro studies. The aim of this study was to investigate effect of Lactobacillus on halitosis by Fusobacterium nucleatum- and Porphyromonas gingivalis-producing VSCs and to analyze inhibitory mechanism. The periodontopathogens were cultivated in the presence or the absence Lactobacillus, and the level of VSCs was measured by gas chromatograph. For analysis of inhibitory mechanisms, the susceptibility assay of the spent culture medium of Lactobacillus against F. nucleatum and P. gingivalis was investigated. Also, the spent culture medium of Lactobacillus and periodontopathogens were mixed, and the emission of VSCs from the spent culture medium was measured by gas chromatograph. L. casei and L. rhamnosus significantly reduced production of VSCs. L. casei and L. rhamnosus exhibited strong antibacterial activity against F. nucleatum and P. gingivalis. The spent culture medium of L. casei inhibited to emit gaseous hydrogen sulfide, methyl mercaptan and dimethyl sulfide from the spent culture medium of periodontopathogens. However, the spent medium of L. rhamnosus repressed only dimethyl sulfide. L. casei ATCC 334 may improve halitosis by growth inhibition of periodontopathogens and reduction of VSCs emission.

• 한국식품조리과학회지
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• 제4권2호
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• pp.65-69
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• 1988

세 가지 액체배지를 사용하여 Berkeley대학 Dr. Bjeldanes Lab에서 isolate된 F. moniliforme을 생육시킨 결과 Czapek-Dox medium에서 Fusarin C생성량이 가장 높았으며 옥수수에 비하면 약 1/10정도의 양인 것으로 나타났다. 그리고 Czapek-Dox medium의 경우 $28^{\circ}C$에서 2주간 배양한 때에 Fusarin C의 생성량이 가장 많았으며 옥수수의 경우는 28$^{\circ}C$에서 1주간 배양한 때에 가장 높았다. 또한 Czapek-Dox medium의 initial PH가 6.5인 때에 Fusarin C의 생성량이 최대이었으며 6.3, 5.9인 때도 다른 PH보다 월등히 높은 양이 생성됨을 확인할 수 있었다.

• Biomolecules & Therapeutics
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• 제24권1호
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• pp.49-56
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• 2016

To determine the protective effect of aloe-emodin (AE) from high glucose induced toxicity in RIN-5F (pancreatic ${\beta}$-cell) cell and restoration of its function was analyzed. RIN-5F cells have been cultured in high glucose (25 mM glucose) condition, with and without AE treatment. RIN-5F cells cultured in high glucose decreased cell viability and increased ROS levels after 48 hr compared with standard medium (5.5 mM glucose). Glucotoxicity was confirmed by significantly increased ROS production, increased pro-inflammatory (IFN-${\gamma}$, IL-$1{\beta}$,) & decreased anti-inflammatory (IL-6&IL-10) cytokine levels, increased DNA fragmentation. In addition, we found increased Bax, caspase 3, Fadd, and Fas and significantly reduced Bcl-2 expression after 48 hr. RIN-5F treated with both high glucose and AE ($20{\mu}M$) decreased ROS generation and prevent RIN-5F cell from glucotoxicity. In addition, AE treated cells cultured in high glucose were transferred to standard medium, normal responsiveness to glucose was restored within 8hr and normal basal insulin release within 24 hr was achieved when compared to high glucose.

• 한국광학회지
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• 제11권5호
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• pp.371-376
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• 2000

디스크 형태의 저장 매체에 다중 홀로그램을 기혹함에 있어서, 우리는 저장밀도를 향상시키기 위해 회전, 각, 그리고 공간 다중화를 복합적으로 구현할 수 있는 간단하고 비용면에서 효율적인 방법을 연구한 바 있다. 회전다중과 각다중은 두 개의 쐐기 프리즘을 이용하여 기준빔을 직접 제어함으로써 얻고, 공간다중은 기록매질을 이동시킴으로써 얻었다. 본 논문에서는 저장밀도가 신호빔과 기준빔 경로상에 있는 렌즈들을 F/#(f number)와 밀접한 관계가 있으며, 그 저장밀도가 신호빔 경로상에 있는 렌즈의 F/#가 기준빔 경로에 있는 렌즈의 F/#의 약 두 배가 될 때 최대로 얻어짐을 보였다.

• KSBB Journal
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• 제8권5호
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• pp.483-487
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• 1993

기존의 무혈청 배지에 첨가하던 BSA/acid를 acid/Pluronic F-68 emulsion으로 대치하여도 세포성장에는 전혀 지장이 없었다. BSA를 제외함으로써 무혈청 배지에 첨가하는 단백질 함량을 최소하할 수 있었고, 이로 인한 세포농도 감소는 없었다. SFLSM은 0.2micron 필터로 여과 멸균하여도 SFLSM의 세포성장효과는 영향을 받지 않는다.

• BMB Reports
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• 제28권5호
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• pp.433-436
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• 1995

In order to assign NMR signals, conditions for the specific labeling of cytochrome $c_3$ of D. vulgaris Miyazaki F through the culture in a minimal medium were established. Phenylalanine residue was specifically deuterated at more than 85% efficiency. Cytochrome $c_3$ has two phenylalanine residues. The signals of one phenylalane were missing and this was tentatively assigned to Phe20.

• 식물조직배양학회지
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• 제26권4호
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• pp.289-291
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• 1999

발아 7일된 오이 (Cucumis sativus L.) F$_1$ 5품종과 순계 4품종의 하배축 절편을 1mg/L 2,4-D가 첨가된 MS고체배지에 치상하여 3주간 배양하였다. F$_1$ 1품종과 순계 2품종에서 발달한 캘러스의 8%이하에서 체세포배가 발생하였다. 체세포 배 절편을 동일한 배지에 4주간 배양하여 배발생캘러스를 유도하였다. 배발생캘러스는 동일조성의 MS액체배지에 현탁되어 증식하였다. 액체배지에서 배발생캘러스는 수많은 체세포 배로 발달하였으며, 생장조절제가 첨가되지 않은 MS기본배지에서 95%이상의 체세포배가 식물체로 발아하였다. 재분화된 식물체는 화분에서 생육되어 개화 후 착과하였다.

• 한국자원식물학회지
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• 제19권3호
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• pp.422-426
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• 2006

This research was conducted to get the basic materials necessary to obtain the somatic hybrid plant between Solanum sisymbriifolium and other Solanum species (S. integrifolium and S. toxicarium). Regarding the formation of colony from the protoplast in S. sisymbriifolium, S. integrifolium and the fused protoplast mixture; for the S. sisymbriifolium, a colony was observed in F medium(Kao medium containing $5.0mg{\cdot}L^{-1}\;NAA,\;1.0mg{\cdot}L^{-1}$ 2,4-D and $1.0mg{\cdot}L^{-1}$ BA); and for the S. integrifolium, in G medium (a half strength MS medium containing 0.03 M sucrose, 0.4 M mannitol, $1.0mg{\cdot}L^{-1}\;NAA,\;1.0mg{\cdot}L^{-1}$ kinetin) respectively. In mixed cultured protoplast after electriofusion treatment, the cell division and colony formation were observed in both media F and G. For the shoot and root formation rate, there was no difference between the parent of each breed and mixed protoplast regardless of the medium. In the fused protoplast mixture of S. sisymbriifolium and S. toxicarium, a colony formation was also observed in both media F and H(a half strength MS medium containing 0.03 M sucrose, 0.4 M mannitol, $1.0mg{\cdot}L^{-1}\;NAA,\;1.0mg{\cdot}L^{-1}$ kinetin); and there was no difference in the shoot and root formation rate between the parent and the mixed protoplast.