• Journal of Animal Science and Technology
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• v.47 no.2
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• pp.147-158
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• 2005

The purpose of this study was to find out the effects that daughters' incomplete lactation records affect sire's breeding values through genetic evaluation using RRTDM(random regression test-day model). First, we estimated genetic parameters and breeding values on sires having complete lactation records of daughter by RRTDM, second, we changed complete lactation records of specific sires into incomplete records by various methods. Third, the breeding values were compared between complete and incomplete records. Finally, this study aimed to find out the methods to minimize the estimation errors of young bulls' breeding values. Data used in this study were collected from the dairy herd improvement program, and a total of 97,562 records were composed of 10,929 first parity with both parents known, since 1999. Breeding values on the daughters from randomly chosen sires were calculated and compared with among 90 day, 150day, and 200 day's incomplete records. For milk yields, sire's ranks of breeding values used by complete lactation records were very different from sire's ranks of breeding values obtained by incomplete lactation records(Rank_90 cut, 150cut, 200 cut).The differences were also obtained between complete lactation records(per305_full) and incomplete lactation record (per_90 cut, 150cut, 200 cut) in breeding values regarding persistency. Especially, the differences between per_90 cut and per305_full were very large(from 1.8 kg to 145kg).

• The Korean Journal of Zoology
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• v.33 no.1
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• pp.6-11
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• 1990

Analysis of genetic structure in Kimpo natural population of Drosophila was carried out by utilizing the deleterious gene on the second chromosome of Drosophila melanogaster. Male flies tested were continuously collected for eight years; in late September 1974 and 1981-1987. The frequency of deleterious gene (lethal plus semilethal) ranged from 27.02% in 1983 to 41.48% in 1987, and the values estimated from the eight years samples are highly signihcent from each other with a homogenety test (X$^2$=52.0157, d.f.=28, P<0.005). Allelic rates ranged from 1.30% in 1981 to 5.03% in 1974. And the effective population size by using the rate of allelism was estimated average at 3, 300 pairs. Elimination rate by homozygous of lethal gene ranged from 0.0004 in 1984 to 0.0019 in 1974, and that is for smaller than mutation rate(0.005) at second chromosome. We suppose that stable frequency (about 20%) lethal genes of D. melanogaster in Kimpo natual population are maintained by invade of P-type mutator factor (P element) versus eliminated in heterozygous and homozygous condition of lethal gene.

• Korean Journal of Veterinary Research
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• v.46 no.2
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• pp.127-133
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• 2006

Pasteurella multocida is a terrible veterinary pathogen that causes widespread infections in husbandry. To induce homologous and/or heterologous immunity against the infections, outer membrane protein Hs (OmpH) in the envelope of different strains of P. multocida are thought to be attractive vaccine candidates. Previously we cloned and characterized a gene for OmpH from pathogenic P. multocida (A : 3) (In Press, Korean J. Microbiol. Biotechnol. 2005, 33, December). The gene is composed of 1,047 nucleotides (nt) coding 348 amino acids (aa) with signal peptide of 20 aa. The truncated ompH, a gene without nt coding for the signal peptide, was generated using pRSET A to name "pRSET A/OmpH-F2". This truncated ompH was well expressed in Escherichia coli BL21 (DE3). Truncated OmpH was purified for induction of immunity against live pathogen of fowl cholera (P. multocida A : 3) in mice. Some $50{\mu}g$ of the purified polypeptide was intraperitoneally injected into mice two times with 10 day interval. Lethal dose ($25{\mu}l$) of live P. multocida A : 3 was determined by directly injecting the pathogen into wild mice (n = 25). To demonstrate the vaccine candidate of the truncated OmpH, the live pathogen ($25{\mu}l$) was challenged with the OmpH-immunized mouse group as well as positive & negative controls (n = 80). The results show that the truncated OmpH can be used for an effective vaccine production to prevent fowl cholera caused by pathogenic P. multocida (A : 3).

• Korean Journal of Food Science and Technology
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• v.29 no.4
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• pp.752-757
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• 1997

Crude papain extracted at optimum condition was purified with an ethanol precipitation method. Four factors of protein recovery method were optimized by response surface methodology (RSM) and the function was expressed in terms of a quadratic polynomial equation. Adequacy of the model equation for optimum response values was tested and optimum conditions of protein recovery were 38.2 mg/mL of protein, ethanol concentration of 40% and precipitation temperature of $-8^{\circ}C$. The experimental value (68.97%) for recovery yield was closed to the predicted value (77.28%) under these conditions.

• Journal of the Korean Data and Information Science Society
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• v.25 no.5
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• pp.1011-1024
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• 2014

This study is to identify the relationship between unsaturated fatty acids, which are indicators of beef flavor, and unsaturated fatty acid biosynthetic enzymes, which are associated with SNPs in the SCD, SREBPs, $PPAR{\gamma}$, FABP4, FASN and LPL in Hanwoo population. For analysis of fatty acid in Hanwoo, we used to Hanwoo steer(n=513). Also, following an analysis of the relationship raised from Gyeongbuk province region. FABP4; g.3977-325 T>C was selected and the distribution of beef grade of g.3977-325 T>C related in field trial was proved very excellent.

• Applied Biological Chemistry
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• v.35 no.5
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• pp.361-366
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• 1992

The antagonistic bacteria, showing distinguished effect against Fusarium oxysporum and Rhizoctonia solani were isolated from the rhizosphares of horticultural plants and identified as Bacillus subtilis. The strains were studied for their chracteristics of biochemistry, physiology, antagonistic effect against plant pathogenic fungi, and growth promoting effect on horticultural plants. The Bacillus thuringiensis(BT) HD-1 toxin gene was introduced into these B. subtilis. The BT toxin genes on chromosome of the bacteria were identified by southern blotting, but its proteins were not detected by SDS-PAGE. These transformed bacteria showed growth promoting effect and showed also insecticidal and antagonistic effects against Bombix mori and fungi F. oxysporum and R. solani but not against nematode Bursaphelenchus xylophilus.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.11 no.6
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• pp.145-154
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• 2012

This paper analyzed the characteristics of traffic accidents in all tunnels on nationwide freeways and selected some various independent variables related to accident occurrence in tunnels. The study aims to develop reliable accident forecasting models using the various dependent variables such as the number of accident (no.), no./km, and no./MVK. Finally, reliable multiple linear regression models were proposed in this paper. This study tested the validity verification of developed models through statistics such as $R^2$, F values, multicollinearity, residual analysis. The paper selected the accident forecasting models considering the characteristics of tunnel accidents and two models were finally proposed according to two groups of tunnel length. In the selected models, natural logarithm of ln(no./MVK) is used for the dependent variable and AADT, vertical slope, and tunnel hight are used for the independent variables. The reliability of two models was proved by the comparison analysis between field data and estimating data using RMSE and MAE. These models may be not only effective in evaluating tunnel safety under design and planning phases of tunnel but also useful to reduce traffic accidents in tunnels and to manage the traffic flow of tunnel.

• FLOWER RESEARCH JOURNAL
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• v.16 no.4
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• pp.295-298
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• 2008

A new Cymbidium cultivar 'White Princess' was released by the National Horticultural Research Institute (NHRI) in 2005. A cross was made in 1995 between 'Cymbidium 2113', light purple colored petal and medium plant sized variety, and 'Lucky Rainbow Randevous', a dark purple petal with red lip and large type variety. The ninety progenies were obtained after planting and acclimatization in green house. In 2000, a line (9526747) was selected and multipliticed after test of its flower color, leaf shape, flower stalk, and vigorous growing habit. After evaluation trial for two growing seasons, the selected line was named as 'White Princess'. The 'White Princess' has white petals (WN 155A) with red lip (RP59D) color and large sized leaves, and flowers (leaf length is about 88 cm and flower diameter is about 8 cm) with semi-erect flower stalks. Blooming started from December to January in optimal culture conditions. High temperature (more than $30^{\circ}C$) and low light intensity(less than 10,000Lux) should be avoided for the appropriate growth.

• Korean Journal of Breeding Science
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• v.42 no.6
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• pp.717-720
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• 2010

A new cultivar of Euphorbia milii, 'Panorama' was developed at Cactus Research Institute, Gyeonggi-do Agricultural Research & Extension Services in 2009. 'Gabi' and 'Maxi' were crossed in 2001 and three $F_1$ seedlings were obtained. Pedigree selection was performed in 2002. One line was finally selected and named as 'Panorama' through the test of specific character from 2007 to 2009. The new cultivar 'Panorama' has green and pink colored bract, and it has 2~3 each of inflorescence number, 2.0 cm of bract width, 4.3 each of lateral shoot and 14.9 cm of plant height. Characters of the cultivar can be maintained by vegetative propagation.

• Phonetics and Speech Sciences
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• v.14 no.2
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• pp.39-54
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• 2022

This study aims to clarify the prosodic differences in speech types by examining the Korean read speech and spontaneous speech in the Korean part of the L2 Korean Speech Corpus (speech corpus for Korean as a foreign language). To this end, the articulation length, articulation speed, pause length and frequency, and the average fundamental frequency values of sentences were set as variables and analyzed via statistical methodologies (t-test, correlation analysis, and regression analysis). The results found that read speech and spontaneous speech were structurally different in the form of prosodic phrases constituting each sentence and that the prosodic elements differentiating each speech type were articulation length, pause length, and pause frequency. The statistical results show that the correlation between articulation speed and articulation length was highest in read speech, explaining that the longer a given sentence is, the faster the speaker speaks. In spontaneous speech, however, the relationship between the articulation length and the pause frequency in a sentence was high. Overall, spontaneous speech produces more pauses because short intonation phrases are continuously built to make a sentence, and as a result, the sentence gets lengthened.