• International Journal of Concrete Structures and Materials
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• v.19 no.1E
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• pp.25-32
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• 2007

The fracture energy evaluated from the previous experimental results can be simulated by using the modified singular fracture process zone (S-FPZ) model. The fracture model has two fracture properties of strain energy release rate for crack extension and crack close stress versus crack width relationship $f_{ccs}(w)$ for fracture process zone (FPZ) development. The $f_{ccs}(w)$ relationship is not sensitive to specimen geometry and crack velocity. The fracture energy rate in the FPZ increases linearly with crack extension until the FPZ is fully developed. The fracture criterion of the strain energy release rate depends on specimen geometry and crack velocity as a function of crack extension. The behaviors of micro-cracking, micro-crack localization and full development of the FPZ in concrete can be explained theoretically with the variation of strain energy release rate with crack extension.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.26 no.9
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• pp.1897-1904
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• 2002

Monotonic torsional and pure torsional low cycle fatigue(LCF) test with artificial degradation were performed on duplex stainless steel(CF8M). CF8M is used in pipes and valves in nuclear reactor coolant system. It was aged at 430$^{\circ}C$ for 3600hrs. Through the monotonic and LCF test, it is found that mechanical properties(i.e., yield strength, strain hardening exponent, strength coefficient etc.) increase and fatigue life(N$\sub$f/) decreases with degradation of material. The relationship between shear strain amplitude(${\gamma}$$\sub$a/)and N$\sub$f/ was proposed.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.25 no.11
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• pp.1569-1580
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• 2001

A new nonlinear near-wall turbulence model is developed to predict turbulent flow and heat transfer in strongly nonequilibrium flows. The k-$\varepsilon$-f$\sub$${\mu}$/, model of Park and Sung$\^$(1)/ is extended to a nonlinear formulation. The stress-strain relationship is the thrid-order in the mean velocity gradients. The strain dependent coefficients are obatined from the realizability constraints and the singular behavior at large strains. An improved explicit heat flux model is proposed with the aid of Cayley-Hamilton theorem. This new model includes the quadratic effects of flow deformations. The near-wall asymptotic behavior is incorporated by modifying the f$\sub$λ/ function. The model performance is shown to be satisfactory.

• Geomechanics and Engineering
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• v.7 no.4
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• pp.431-458
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• 2014

Construction of a new cavern close to an existing cavern will result in a modification of the state of stresses in a zone around the existing cavern as interaction between the twin caverns takes place. Extensive plane strain finite difference analyses were carried out to examine the deformations induced by excavation of underground twin caverns. From the numerical results, a fairly simple nonparametric regression algorithm known as multivariate adaptive regression splines (MARS) has been used to relate the maximum key point displacement and the percent strain to various parameters including the rock quality, the cavern geometry and the in situ stress. Probabilistic assessments on the serviceability limit state of twin caverns can be performed using the First-order reliability spreadsheet method (FORM) based on the built MARS model. Parametric studies indicate that the probability of failure $P_f$ increases as the coefficient of variation of Q increases, and $P_f$ decreases with the widening of the pillar.

• Journal of Microbiology and Biotechnology
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• v.17 no.12
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• pp.1937-1943
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• 2007

A new strain, GS603, having ${\beta}$-glucosidase activity was isolated from soil of a ginseng field, and its ability to convert major ginsenoside $Rb_1$ to minor ginsenoside or gypenoside was studied. Strain GS603 was identified as an Intrasporangium species by phylogenetic analysis and showed high ginsenoside-converting activity in LB and TSA broth but not in nutrient broth. The culture broth of the strain GS603 could convert ginsenoside $Rb_1$i into two metabolites, which were analyzed by TLC and HPLC and shown to be the minor ginsenoside $F_2$ and gypenoside XVII by NMR.

• Proceedings of the Korean Geotechical Society Conference
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• 2005.03a
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• pp.916-923
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• 2005

In this study, to observe aging effect of undrained shear behavior for Nak-Dong River sand, undrained static and cyclic triaxial tests were performed with changing relative density ($D_r$), consolidation stress ratio($K_c$) and consolidation time. As a result of the test, the modulus of elasticity to all samples estimated within elastic zone by the micro strain of about 0.05% in case of static shear behavior increased with the lapse of consolidation time significantly, so aging effect was shown largely. Also strength of phase transformation point(S_{PT}$) and strength of critical stress ratio point($S_{CSR}$) increased with the lapse of consolidation time. Undrained cyclic shear strength($R_f$) obtained from the failure strain 5% increased in proportion to relative density($D_r$) and initial static shear stress($q_{st}$), $R_f$ of consolidated sample for 1,000 minutes increased about 10.6% compared to that for 10 minutes at the loose sand, and $R_f$ increased about 7.0% at the medium sand. In situ application range of $R_f$ to the magnitude of earthquake for Nak-Dong River sand was proposed by using a increasing rate of $R_f$ as being aging effect shown from this test result.

• Journal of Microbiology and Biotechnology
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• v.12 no.5
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• pp.819-825
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• 2002

Methanol dehydrogenase (MDH) is a key enzyme in the process of methanol oxidation in methylotrophic bacteria. However, information on MDH genes from genus Methylobacillus is limited. In this study, a 6.5-kb HindIII DNA fragment of Methylobacillus sp. SK-5 chromosomal DNA was isolated from the genomic library of the strain by using a degenerate oligonucleotide probe that was designed based on JV-terminal amino acid sequence of the MDH $\alpha$ subunit purified from the strain. Molecular analysis of the fragment revealed four tightly clustered genes (mxaFJGI) involved in the methanol oxidation. The first and fourth genes were very similar to mxaF (77% identity for nucleotides an 78% identity for amino acids) and mxaF (67% Identity for nucleotides and 68% Identity for amino acids) genes, respectively, from Methylovorus sp. SSI. Genes mxaF and mxaI encode $\alpha$ and $\beta$ subunits of MDH, respectively. The two subunits were identified from purified MDH from Methylobacillus sp. SK-5. A dendrogram constructed by comparison of amino acid sequences of MDH u subunits suggests that MxaF from Methylobacillus sp. SK-5 belongs to a subfamily cluster of MDH u subunits from $\beta$-subgroup Proteobacteria. The subfamily cluster is separated from the other subfamily that consists of $\beta$- and $\gamma$-subgroup Proteobacteria. This study provided information on mn genes from a methylotrophic bacterium in $\beta$-subgroup Proteobacteria, which would aid to better develop a gene probe to detect one-carbon metabolizing bacteria.

• The Plant Pathology Journal
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• v.29 no.1
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• pp.52-58
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• 2013

Fusarium head blight (FHB) caused by the filamentous fungus Fusarium graminearum is one of the most severe diseases threatening the production of small grains. Infected grains are often contaminated with mycotoxins such as zearalenone and trichothecences. During survey of contamination by FHB in rice grains, we found a bacterial isolate, designated as BN1, antagonistic to F. graminearum. The strain BN1 had branching vegetative hyphae and spores, and its aerial hyphae often had long, straight filaments bearing spores. The 16S rRNA gene of BN1 had 100% sequence identity with those found in several Streptomyces species. Phylogenetic analysis of ITS regions showed that BN1 grouped with S. sampsonii with 77% bootstrap value, suggesting that BN1 was not a known Streptomyces species. In addition, the efficacy of the BN1 strain against F. graminearum strains was tested both in vitro and in vivo. Wheat seedling length was significantly decreased by F. graminearum infection. However, this effect was mitigated when wheat seeds were treated with BN1 spore suspension prior to F. graminearum infection. BN1 also significantly decreased FHB severity when it was sprayed onto wheat heads, whereas BN1 was not effective when wheat heads were point inoculated. These results suggest that spraying of BN1 spores onto wheat heads during the wheat flowering season can be efficient for plant protection. Mechanistic studies on the antagonistic effect of BN1 against F. graminearum remain to be analyzed.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2009.06a
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• pp.245-246
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• 2009

정전용량형 후막 스트레인 게이지(piezocapacitive thick film strain gage)는 세라믹 ($Al_2O_3$)을 주 원료로 하는 지지대(약 5mm)와 다이어프램(약 $300{\mu}m$) 그리고 가드 링으로 구성된다. 전극 판은 도전성 페이스트를 이용하여 지지대와 다이어프램에 형성되었으며 극판 사이에는 유전체 메이스트를 사용하여 스크린 인쇄로 후막을 형성하였다. 극판 사이의 가드 링 두께는 약 $30{\mu}m$정도로 다이어프램의 변위 최대값을 유지시키는 데 필요한 간격이다. 따라서 정전용랑형 후막 스트레인 게이지는 지지대를 중심으로 다이어프램에 압력 (0.5~1.0bar)이 인가될 때 변위를 발생시키면서 커패시터 값이 압력의 크기에 따라 비례 특성을 가지고 변화하는 것을 이용한 것이다. 압력이 없을때 초기값은 35pF~40pF 정도이고 정격압력의 최대치를 인가시켰을 때 약 55pF~55p를 나타내었다.

• Mycobiology
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• v.42 no.3
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• pp.256-261
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• 2014

A ${\beta}$-glucosidase producing yeast strain was isolated from Korean traditional rice wine. Based on the sequence of the YCL008c gene and analysis of the fatty acid composition, the isolate was identified as Saccharomyces cerevisiae strain HJ-014. S. cerevisiae HJ-014 produced ginsenoside Rd, $F_2$, and compound K from the ethanol extract of red ginseng. The production was increased by shaking culture, where the bioconversion efficiency was increased 2-fold compared to standing culture. The production of ginsenoside $F_2$ and compound K was time-dependent and thought to proceed by the transformation pathway of: red ginseng extract ${\rightarrow}Rd{\rightarrow}F_2{\rightarrow}$ compound K. The optimum incubation time and concentration of red ginseng extract for the production of compound K was 96 hr and 4.5% (w/v), respectively.