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Microbial Conversion of Ginsenoside $Rb_1$ to Minor Ginsenoside $F_2$ and Gypenoside XVII by Intrasporangium sp. GS603 Isolated from Soil  

Cheng, Le-Qin (Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University)
Na, Ju-Ryun (Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University)
Kim, Myung-Kyum (Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University)
Bang, Myun-Ho (Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University)
Yang, Deok-Chun (Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University)
Publication Information
Journal of Microbiology and Biotechnology / v.17, no.12, 2007 , pp. 1937-1943 More about this Journal
Abstract
A new strain, GS603, having ${\beta}$-glucosidase activity was isolated from soil of a ginseng field, and its ability to convert major ginsenoside $Rb_1$ to minor ginsenoside or gypenoside was studied. Strain GS603 was identified as an Intrasporangium species by phylogenetic analysis and showed high ginsenoside-converting activity in LB and TSA broth but not in nutrient broth. The culture broth of the strain GS603 could convert ginsenoside $Rb_1$i into two metabolites, which were analyzed by TLC and HPLC and shown to be the minor ginsenoside $F_2$ and gypenoside XVII by NMR.
Keywords
Bioconversion; ginsenoside $F_2$; gypenoside XVII; Intrasporangium; ginsenoside $Rb_1$;
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Times Cited By KSCI : 7  (Citation Analysis)
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1 Cheng, L. Q., M. K. Kim, J. W. Lee, Y. J. Lee, and D. C. Yang. 2006. Conversion of major ginsenoside Rb1 to ginsenoside $F_2$ by Caulobacter leidyia. Biotechnol. Lett. 28: 1121-1127   DOI   ScienceOn
2 Lee, D. S., Y. S. Kim, C. N. Ko, K. H. Cho, H. S. Bae, K. S Lee, J. J. Kim, E. K. Park, and D. H. Kim. 2002. Fecal metabolite activities of herbal components to bioactive compounds. Arch. Pharm. Res. 25: 165-169   DOI
3 Sato, K., M. Mochizuki, I. Saiki, Y. C. Yoo, K. Samukawa, and I. Azuma. 1994. Inhibition of tumor angiogenesis and metastasis by a saponin of Panax ginseng-ginsenoside $Rb_2$. Biol. Pharm. Bull. 17: 635-639   DOI   ScienceOn
4 Yu, H., H. Liu, C. Zhang, D. Tan, M. Lu, and F. Jin. 2004. Purification and characterization of gypenoside-${\alpha}$ -L-rhamnosidase hydrolyzing gypenoside-5 to ginsenoside Rd. Process Biochem. 39: 861-867   DOI   ScienceOn
5 Coskun, H. and E. Öndül. 2004. Free fatty acid accumulation by mesophilic lactic acid bacteria in cold-stored milk. J. Microbiol. 42: 133-138
6 Kim, M. K., J. W. Lee, K. Y. Lee, and D.-C. Yang. 2005. Microbial conversion of major ginsenoside Rb1 to pharmaceutically active minor ginsenoside Rd. J. Microbiol. 43: 456-462   과학기술학회마을
7 Lin, M. C., K. C. Wang, and S. S. Lee. 2001. Transformation of ginsenosides Rg1 and Rb1 and crude Sanchi saponins by human intestinal microflora J. Chin. Chem. Soc. 48: 113- 120   DOI
8 Xie, J. T., S. R. Mehendale, X. Li, R. Quigg, X. Wang, C. Z. Wang, J. A. Wu, H. H. Aung, P. A. Rue, G. I. Bell, and C. S. Yuan. 2005. Anti-diabetic effect of ginsenoside Re in ob/ob mice. Biochim. Biophys. Acta 1740: 319-325   DOI   ScienceOn
9 Chi, H., B. H. Lee, H. J. You, M. S. Park, and G. E. Ji. 2006. Differential transformation of ginsenosides from Panax ginseng by lactic acid bacteria. J. Microbiol. Biotechnol. 16: 1629-1633   과학기술학회마을
10 Saito, H., Y. Yoshida, and K. Takagi. 1974. Effect of Panax ginseng root on exhaustive exercise in mice. Jpn. J. Pharmacol. 24: 119-127   DOI
11 Kim, M.-W., S.-R. Ko, K.-J. Choi, and S.-C. Kim. 1987. Distribution of saponin in various sections of Panax ginseng root and changes of its contents according to root age. Korean J. Ginseng Sci. 11: 10-16
12 Yoon, K. Y., E. E. Woodams, and Y. D. Hang. 2004. Probiotication of tomato juice by lactic acid bacteria. J. Microbiol. 42: 315-318
13 Kim, S.-J., C.-M. Lee, M.-Y. Kim, Y.-S. Yeo, S.-H. Yoon, H.-C. Kang, and B.-S. Koo. 2007. Screening and characterization of an enzyme with ${\beta}$-glucosidase activity from environmental DNA. J. Microbiol. Biotechnol. 17: 905-912   과학기술학회마을
14 Wang, B. X., J. C. Cui, A. J. Liu, and S. K. Wu. 1983. Studies on the anti-fatigue effect of the saponins of stems and leaves of Panax ginseng (SSLG). 1983. J. Tradit. Chin. Med. 3: 89-94
15 Lee, B. H., H. J. You, M. S. Park, B. K., and G. E. Ji. 2006. Transformation of the glycosides from food materials by probiotics and food microorganisms. J. Microbiol. Biotechnol. 16: 497-504   과학기술학회마을
16 Zhang, C., H. Yu, Y. Bao, L. An, and F. Jin. 2002. Purification and characterization of ginsenoside-${\alpha}$-arabinofuranase hydrolyzing ginsenoside Rc to Rd from the fresh root of Panax ginseng. Process Biochem. 37: 793-798   DOI   ScienceOn
17 Park J. H. 2004. Sun ginseng - a new processed ginseng with fortified activity. Food Ind. Nutr. 9: 23-27
18 Bae, E. A., N.-Y. Kim, M. J. Han, M.-K. Choo, and D.-H. Kim. 2006. Transformation of ginsenosides to compound K (IH-901) by lactic acid bacteria of human intestine. J. Microbiol. Biotechnol. 13: 9-14
19 Shin, Y.-W., E.-A. Bae, M. J. Han, and D.-H. Kim. 2006. Metabolism of ginsenoside Rg5, a main constituent isolated from red ginseng, by human intestinal microflora and their antiallergic effect. J. Microbiol. Biotechnol. 16: 1791- 1798   과학기술학회마을
20 Dong, A. L., M. Ye, H. Z. Guo, J. H. Zheng, and D. Guo. 2003. Microbial transformation of ginsenoside Rb1 by Rhizopus stolonifer and Curvularia lunata. Biotechnol. Lett. 25: 339-344   DOI   ScienceOn
21 Kim, D. J., G. S. Seong, D. W. Kim, S. L. Go, and J. C. Jang. 2004. Antioxidative effects of red ginseng saponins on paraquat-induced oxidative stress. Korean J. Ginseng Res. 28: 5-10   DOI
22 Han, B. H., M. H. Park, Y. N. Han, W. S. Woo, U. Sankawa, S. Yahara, and O. Tanaka. 1982. Degradation of ginseng under mild acidic condition. Plant Med. 44: 146-149   DOI   ScienceOn
23 Bae, E. A., S. Y. Park, and D. H. Kim. 2000. Constitutive ${\beta}$-glucosidases hydrolyzing ginsenoside Rb1 and Rb2 from human intestinal bacteria. Biol. Pharm. Bull. 23: 1481- 1485   DOI   ScienceOn
24 Mochizuki, M., Y. C. Yoo, and K. Matsuzawa. 1995. Inhibitory effect of tumor metastasis in mice by saponins, ginsenoside $Rb_2$, 20(R)- and 20(S)-ginsenoside $Rg_3$, of Red ginseng. Biol. Pharm. Bull. 18: 1197-1202   DOI   ScienceOn