• Journal of Ginseng Research
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• 제42권3호
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• pp.343-351
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• 2018

Background: Red ginseng is a popularly used traditional medicine with antiaging effects in Asian countries. The present study aimed to explore the changes in protein expression underlying the mechanisms of life span extension and antiaging caused by red ginseng extract (RGE) in Drosophila melanogaster. Methods: A proteomic approach of two-dimensional polyacrylamide gel electrophoresis (2-DE) was used to identify the differential abundance of possible target proteins of RGE in D. melanogaster. The reliability of the 2-DE results was confirmed via Western blotting to measure the expression levels of selected proteins. Proteins altered at the expression level after RGE treatment (1 mg/mL) were identified by matrix-assisted laser desorption/ionization-time of flight tandem mass spectrometry and by searching against the National Center for Biotechnology nonredundant and Uniprot protein databases. The differentially expressed proteins were analyzed using bioinformatics methods. Results: The average survival life span of D. melanogaster was significantly extended by 12.60% with RGE treatment (1 mg/mL) compared to untreated flies. This followed increased superoxide dismutase level and decreased methane dicarboxylic aldehyde content. Based on the searching strategy, 23 differentially expressed proteins were identified (16 up-regulated and 7 down-regulated) in the RGE-treated D. melanogaster. Transduction pathways were identified using the Kyoto Encyclopedia of Genes and Genomes database, and included the hippo and oxidative phosphorylation pathways that play important roles in life span extension and antiaging process of D. melanogaster. Conclusion: Treatment with RGE in D. melanogaster demonstrated that mechanisms of life span extension and antiaging are regulated by multiple factors and complicated signal pathways.

• Journal of Ginseng Research
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• 제31권4호
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• pp.210-216
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• 2007

The backbone structure of ginsenosides, active ingredients of Panax ginseng, is similar with that of sterol, especially cholesterol. Caenorhabditis elegans (c. elegans) is one of free living nematodes and is well-established animal model for biochemical and genetic studies. C. elegans cannot synthesize de novo cholesterol, although cholesterol is essential requirement for its growth and development. In the present study, we investigated the effects of Korean red ginseng total extract (KRGE), ginseng total saponins (GTS) on life span of C. elegans in cholesterol-deprived and -fed medium. Cholesterol deprivation caused damages on life span of worms throughout F1 to F3 generations. KRGE or GTS supplement to cholesterol-deprived medium restored the life span of worms as much as cholesterol alone-fed medium. In study to identify which ginsenosides are responsible for life span restoring effects of KRGE, we found that ginsenoside Rc supplement not only restored life span of worms grown in cholesterol-deprived medium but also prolonged life span of worms grown in cholesterol-fed medium. These results show a possibility that ginsenosides could be utilized by C. elegans as a sterol substitute and further indicate that ginsenoside Rc is the effective component of Korean red ginseng that prolongs the life span of C. elegans.

• 생명과학회지
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• 제14권2호
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• pp.209-214
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• 2004

본 연구에서는 키토산이 암세포에 미치는 효과를 보기 위하여 암세포에 대한 세포독성효과, 키토산을 기존 항암제와 함께 사용하였을 때의 암세포에 대한 세포독성효과 및 암마우스에 대한 생명연장 효과의 변화를 관찰하여 암 치료 가능성을 조사하고자 하였다. 암세포인 K562세포나 Yac-1세포에 키토산을 단독으로 작용시켰을 때 암세포성장억제효과를, 기존항암제(mitomycin C, cisplatin, 5-fluorouracil)와 복합으로 작용시켰을 때 암세포성장억제효과의 상승효과를 보이고 암 마우스에서 키토산을 기존항암제와 복합으로 투여한 결과 생명연장효과를 보였다. 따라서 이러한 결과들은 앞으로의 추가적인 연구결과들이 있게 되면 임상에서의 암 치료에 키토산의 이용가능성을 시사한다 하겠다.

• 한국산학기술학회논문지
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• 제9권2호
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• pp.281-285
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• 2008

냉간단조금형(Cold Forging Die)의 다이블록(Dieblock)을 제작하는 방법 중의 하나로, 다이블록 제작용 재료를 면가공 하여 다이블록 상면(上面)을 마스터펀치(Master Punch)인 호브(Hob)로 압입(Indentation) 시켜 절삭가공((Cutting Work)이 아닌 다이호빙(Die Hobbing) 방법으로 임프레션(Impression)을 성형하여 제작하고 있다. 이 방법에 의하여 다이블록의 재료를 합금공구강(Alloy Tool Steel)인 SKD11을 사용하여 제작하고, 스테인리스판(Stainless Sheet Metal)을 제품 재료로 하여 냉간단조가공(Cold Forging Work)을 수행하였더니 6,000 스트로크(Stroke)에서 금형수명(Die Life)을 다 하고 파손되었다. 본 논문에서는 다이블록 재료를 고속도공구강(High Speed Tool Steel)인 SKH51로 교체 제작하고, 탄소강(Carbon Steel)인 S45C를 제품 재료로 하여 냉간단조가공을 수행하였더니 21,000 스트로크에서 금형수명을 다하고 파손되어 종래의 방법과 비교 검토하였을 때 350%의 금형수명 연장 효과를 얻게 되었다.

• Molecules and Cells
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• 제26권3호
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• pp.299-307
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• 2008

To characterize gene expression that is dependent on the strength of calorie restriction (CR), we obtained transcriptome at different levels of glucose, which is a major energy and carbon source for budding yeast. To faithfully mimic mammalian CR in yeast culture, we reconstituted and grew seeding yeast cells in fresh 2% YPD media before inoculating into 2%, 1%, 0.5% and 0.25% YPD media to reflect different CR strengths. We collected and characterized 160 genes that responded to CR strength based on the rigorous statistical analyses of multiple test corrected ANOVA (adjusted p value < 0.1 or raw p value < 0.0031) and Pearson correlation (|r| > 0.7). Based on the individual gene studies and the GO Term Finder analysis of 160 genes, we found that CR dose-dependently and gradually increased mitochondrial function at the transcriptional level. Therefore, we suggest these 160 genes are markers that respond to CR strength and that might be useful in elucidating CR mechanisms, especially how stronger CR extends life span more.

• 한국도로학회:학술대회논문집
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• 한국도로학회 2005년도 춘계학술대회 논문집
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• pp.27-34
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• 2005

• 한국철도학회:학술대회논문집
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• 한국철도학회 2010년도 춘계학술대회 논문집
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• pp.775-783
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• 2010

새마을 동차를 유지보수하는 용산기지의 이전에 따른 새마을 동차를 유지보수 할 기지가 없어, 현재 내구연한 20년인 새마을동차의 조기폐차가 논의 되고 있지만, 철도차량의 도입에 많은 비용이 소요되고, 정확한 정책판단이 요청되어 경제성 분석을 시행하였다. 분석을 위해 기존의 PP동차를 조기 폐차하는 경우와 신규간선형 EMU를 도입하는 경우, 철도차량정밀진단을 통해 PP동차를 내구연한을 초과하여 사용하는 경우를 시나리오 설정을 통해서 경제성분석을 시도하였다. 경제성 분석을 위해서 크게 유지보수비, 운영비, 차량감가상각비(차량 잔존가액)와 조기폐차에 따른 부대적인 비용을 산정하여 분석하였다. 특정한 철도공사의 사례이지만, 디젤차량과 전기차량의 운영비적인 측면에서 상세한 검토자료가 될 수 있다.

• 농촌지도와개발
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• 제11권1호
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• pp.1-19
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• 2004

The objective of this study was to find a linkage of ideological background between agricultural extension education and lifelong education. This study was conducted by analyzing the studies related to agricultural extension and lifelong education. Review of literature and documents was main methods of this study. The study reviewed and analyzed the concepts, characteristics and ideology of lifelong education, and presented some general characteristics of lifelong education in the context of educational ideology. As a result of the study, the following five characteristics of lifelong education in the context of educational ideology were presented; 1) lifelong education is the supreme concept of education and includes all kinds of education, 2) lifelong education is the future direction of educational ideology and philosophy rather than a kind of educational practice, 3) lifelong education means the security for a right of learning through the entire life-span of an individual, 4) lifelong education has the innovative function of the existing situation of education; viewpoint, contents, and methodology of learning, 5) Lifelong education runs ultimately towards a 'learning society'. Agricultural extension and lifelong education shared the similar ideological background in general, and have the similar basic philosophy. The ideology and philosophy of lifelong education should be reflected into the ideology of agricultural extension to broaden the perspectives of agricultural extension in the future.

• 생약학회지
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• 제39권4호
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• pp.310-315
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• 2008

Viscum album coloratum (Korean mistletoe; KM) is a semi-parasitic plant growing on various trees. This plant hasbeen shown to possess a variety of biological functions such as immunomodulation, apoptosis-induction and antitumor activity. However, there is no information about how the extract of KM affects life span of the host. In this study, in order to examine the effect of KM on the longevity of the host, we investigated whether a Korean mistletoe extract (KME) was able to extend the life length in an experimental model using the spring silkworm moth of Bombyx mori (Lepidoptera bombycidae). We established a model test for survival in which all of the male and female moths died within 18 days after the beginning of the experiment. Mean survival time of female moths was longer than that of male moths. Both male and female moths fed with KME containing diet (200 or 400 ${\mu}g$/head/day) showed significantly higher mean survival times than those of the control moths. Studying the effect of KME on prolongation of mean survival time showed that male moths were more susceptible than female moths. The moths fed with KME-containing diet showed a slight decrease in body weight. Interestingly, however, no difference in food intake was observed between moths fed with KME-containing diet and those with control diet. In analysis for mRNA increase in the SIRT2 gene, a member of the Sir2 gene family playing important roles in regulation of cell death and prolongation of life span, moths fed with KME-containing diet showed a significant increase in SIRT2 gene expression. These results suggest that KME has a potential to extend the life span in Bombyx mori, and its effect is partly associated with increase in SIRT2 gene expression.

• 대한한의학방제학회지
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• 제16권1호
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• pp.55-64
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• 2008

The span of life of human beings has lengthened because of medical progress and so on. Consequently, the old have begun to remark that quality of life (QOL) is as important as extension of the life expectancy. However, some diseases such as stroke, cancer induce decrease of QOL. In case of the stroke, it is followed by chronic headache and dementia as an aftereffect, causing serious decrease of QOL. Jodeungsan, traditional medicine, have been prescribed for headache and hypertension. Many clinical trials and laboratory experiments about pharmacological effects of Jodeungsan have been reported. Jodeungsan ameliorated chronic headache and improved recognitive deficit. Moreover, Jodeungsan lowered blood pressure in hypertensive condition which is one of a major cause of stroke. These effects of Jodeungsan on several diseases are partly attributed to antioxidant effect. This report reviews the pharmacological effect of Jodeungsan in the view of stroke-related diseases.