• Journal of Applied Biological Chemistry
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• v.61 no.4
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• pp.379-382
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• 2018

Trafficking process of viral glycoprotein to cell surface results in the syncytium formation when baby hamster kidney (BHK) cells was infected by Newcastle disease virus (NDV). Rhus chinensis gall, well-known as a medicinal plant, inhibited not only syncytium formation, but also trafficking of glycoprotein, hemagglutinin-neuramidase (HN) to the cell-surface. Modification of viral glycoprotein is processed within the endoplasmic reticulum and golgi body during trafficking into surface. R. chinensis gall extracts showed the strong inhibitory activities ($IC_{50}$ $12.5{\mu}g/mL$) against ${\alpha}-glucosidase$, when compared with the ${\beta}-glucosidase$. And this inhibitory activities is increased by the samples in a dose-depedent pattern. These data showed that the extracts of R. chinensis gall inhibited the cell-surface expression of NDV-hemagglutinin-neuramidase glycoprotein without significantly affecting HN glycoprotein synthesis in NDV-infected BHK cells.

• Parasites, Hosts and Diseases
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• v.57 no.3
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• pp.217-223
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• 2019

Acanthamoeba castellanii has ubiquitous distribution and causes primary acanthamoebic keratitis (AK). AK is a common disease in contact lens wearers and results in permanent visual impairment or blindness. In this study, we observed the cytopathic effect, in vitro cytotoxicity, and secretion pattern of cytokines in human corneal epithelial cells (HCECs) induced by A. castellanii trophozoites and/or cysts. Morphological observation revealed that panked dendritic HCECs co-cultured with amoeba cysts had changed into round shape and gradually died. Such changes were more severe in co-culture with cyst than those of co-cultivation with trophozoites. In vitro cytotoxicity assay revealed the highest cytotoxicity to HCECs in the co-culture system with amoeba cysts. A. castellanii induced the expression of $IL-1{\alpha}$, IL-6, IL-8, and CXCL1 in HCECs. Secreted levels of $IL-1{\alpha}$, IL-6, and IL-8 in HCECs co-cultured with both trophozoites and cysts were increased at an early incubation time (3 and 6 hr). These results suggested that cytopathic changes and pro-inflammatory cytokines release of HCECs in response to A. castellanii, especially amoebic cysts, are an important mechanism for AK development.

• Journal of Fashion Business
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• v.23 no.4
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• pp.13-23
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• 2019

This study aims to understand the concept of pixel, the most important factor in constituting a digital image, draw the formative characteristics of pixelation image expressed through non-digital media, and develop fashion design reflecting the characteristics. As a research method, the literature review was conducted in the present study by involving domestic and foreign publications, related academic journals, and theses and dissertations on the pixel and pixelation image based on a qualitative research process. In addition, through an analysis of the cases that borrowed pixelation images in non-digital media like contemporary art and design, etc., an attempt was made to draw the formative characteristics of the pixelation image. Apparently, six fashion design looks are presented in the present study. The formative characteristics of the pixelation image include: first, the repeatability that repeats the minimum unit; second, the incompleteness of the shape appearing through the phenomenon of aliasing due to the characteristics of the pixel; and third, the combination that completes the shape through the combination of individual independent pixels. The results of the expression through reflecting them in fashion design are as follows: first, this study chose one small geometric formative element and presented repeatability by repetitively expressing that element in a textile pattern; second, for incompleteness, this study expressed an incomplete form, handling the edge part of the shape with the method of disentangling the strand; and third, the combination by completing a single look through overlapping of independent textiles and the combination of different independent individuals is expressed.

• Korean Journal of Agricultural Science
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• v.48 no.3
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• pp.643-654
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• 2021

Phytophthora capsici is one of the most destructive hemibiotrophic pathogens; it can cause blight in chili peppers, and secrete various effector proteins to infect the plants. These effectors contain an N-terminal conserved RXLR motif. Here, we generated full-length RXLR effector coding genes using primer pairs, and cloned them into the pGR106 vector for in planta expression. Two of these genes, PcREK6 and PcREK41 (P. capsici RXLR effector from the Korea isolate), were further characterized. PcREK6 and PcREK41 genes showed that they encode effector proteins with a general modular structure, including the N-terminal conserved RXLR-DEER motif and signal peptide sequences. PcREK6 and PcREK41 expressions were strongly induced when the chili pepper plants (Capsicum annuum) were challenged with P. capsici. These results provide molecular evidence to elucidate the virulence or avirulence factors in chili pepper. Our results also showed that two effectors induce hypersensitive response (HR) cell death when expressed in chili leaves. Cell death suppression assays in Nicotiana benthamiana revealed that most effectors could not suppress programmed cell death (PCD) triggered by Bcl-associated X (BAX) or Phytophthora infestans elicitin (INF1). However, PcREK6 fully suppressed PCD triggered by BAX, while PcREK41 partially suppressed PCD triggered by INF1 elicitin. These results suggest that PcREK effectors from P. capsici interact with putative resistance (R) proteins in planta, and different effectors may target different pathways in a plant cell to suppress pattern-triggered immunity (PTI) or effector-triggered immunity (ETI).

• Journal of Microbiology and Biotechnology
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• v.31 no.2
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• pp.250-258
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• 2021

Among various species of marine bacteria, those belonging to the genus Halomonas have several promising applications and have been studied well. However, not much information has been available on their antibiotic resistance. In our efforts to learn about the antibiotic resistance of strain Halomonas socia CKY01, which showed production of various hydrolases and growth promotion by osmolytes in previous study, we found that it exhibited resistance to multiple antibiotics including kanamycin, ampicillin, oxacillin, carbenicillin, gentamicin, apramycin, tetracycline, and spectinomycin. However, the H. socia CKY01 resistance pattern to kanamycin, gentamicin, apramycin, tetracycline, and spectinomycin differed in the presence of 10% NaCl and 1% NaCl in the culture medium. To determine the mechanism underlying this NaCl concentration-dependent antibiotic resistance, we compared four aminoglycoside resistance genes under different salt conditions while also performing time-dependent reverse transcription PCR. We found that the aph2 gene encoding aminoglycoside phosphotransferase showed increased expression under the 10% rather than 1% NaCl conditions. When these genes were overexpressed in an Escherichia coli strain, pETDuet-1::aph2 showed a smaller inhibition zone in the presence of kanamycin, gentamicin, and apramycin than the respective control, suggesting aph2 was involved in aminoglycoside resistance. Our results demonstrated a more direct link between NaCl and aminoglycoside resistance exhibited by the H. socia CKY01 strain.

• Korean Journal of Construction Engineering and Management
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• v.23 no.1
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• pp.37-45
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• 2022

It is very important to measure the displacement of a structure to evaluate the safety of the structure. This study shows a methodology to measure the displacement to determine the stability of a structure when it is damaged by loads. The methodology used Moiré's phenomenon and was verified through experiments. The experiments utilized pipes to simulate the pipe supports in the construction site and measured the vertical displacement of the Moiré interference patterns according to the horizontal displacement of the pipes. Experiments confirmed that the linear relationship between horizontal displacement of pipes and vertical displacement of Moiré patterns and derive a relational expression. In conclusion, the methodology presented in this work allows us to simultaneously measure a number of vertical members' displacements regardless of distance and determine the safety of the structure.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.26 no.6
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• pp.859-864
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• 2022

Drowsy driving, one of the biggest causes of traffic accidents every year, is accompanied by various factors. As a general method to check whether or not there is drowsiness, a method of identifying a driver's expression and driving pattern, and a method of analyzing bio-signals are being studied. This paper proposes a driver fatigue detection system using deep learning technology and bio-signal measurement technology. As the first step in the proposed method, deep learning is used to detect the driver's eye shape, yawning presence, and body movement to detect drowsiness. In the second stage, it was designed to increase the accuracy of the system by identifying the driver's fatigue state using the pulse wave signal and body temperature. As a result of the experiment, it was possible to reliably determine the driver's drowsiness and fatigue in real-time images.

• Journal of Microbiology and Biotechnology
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• v.32 no.7
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• pp.877-884
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• 2022

Probiotics are microorganisms that can benefit host health when ingested in a live state, and lactic acid bacteria are the most common type. Among fungi, Saccharomyces boulardii (SB) is the only strain known to have a probiotic function with beneficial effects on colitis; however, information on other probiotic yeast strains is limited. Therefore, this study aimed to discover yeast strains expressing intestinal anti-inflammatory activities by exhibiting probiotic properties in dextran sodium sulfate (DSS)-induced colitis mice model. Nuruk (Korean traditional fermentation starter) containing various microbial strains was used as a source for yeast strains, and S. cerevisiae 28-7 (SC28-7) strain was selected with in vitro and in vivo characteristics to enable survival in the intestines. After 14 days of pretreatment with the yeast strains, DSS was co-administered for six days to induce colitis in mice. The results revealed that the disease activity index score was lowered by SC28-7 treatment compared to the DSS group, and the colon length and weight/length ratio were recovered in a pattern similar to that of the normal group. SC28-7 administration significantly reduced the secretion of pro-inflammatory cytokines in the serum and modified the mRNA expression of inflammatory cytokines (interleukin-1β, transforming growth factor-β, and interferon-γ) and proteins involved in gut barrier functions (mucin 2, mucin 3, zonula occludens-1, and occludin) in colon tissues. These results indicate that SC28-7 attenuates DSS-induced colon damage and inflammation, supporting its future use as a probiotic yeast for treating and preventing intestinal inflammatory diseases such as inflammatory bowel disease.

• Journal of Korean Medical classics
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• v.34 no.2
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• pp.207-223
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• 2021

Objectives : This paper studied the different disease sites according to Yin and Yang deviation and the principal to applying the Five Shu Points acupuncture method to these sites of the Yin-Yang needling method as explained in the Shouyaogangrou chapter of the Lingshu. Methods : Of the seasonal needling method in the Neijing, the principle to managing the Five Shu Points and indications of each point were examined. Next, clinical applications of the Five Shu Points were analyzed. Descriptions of clinical expression were collected and examined to understand the underlying pattern. Results : If we connect the disease sites of the Yin-Yang needling method to the Five Shu Points needling method according to the seasonal changes, the Yang of Yin connects to Spring, Yin of Yang to Summer, Yang of Yang to Autumn, and Yin of Yin to Winter. Of the needling site, the collateral vessel is the collateral vessel of Spring, 'Meridian of Yin' is the meridian of the Yin domain or the meridian points of the Yin meridian. 'He of Yang' is the He point of the Yang meridian, and the Yin Xing and Shu are the Xing and Shu points of the Yin meridian. Upon examining cases in the Neijing where the Five Shu Points were applied, it could be found that the Xing and Shu points of the Yin meridian were used together, while the He point of the Yang meridian was used to eliminate Yang pathogen or to stimulate Yang qi, which matches the Yin-Yang needling method of the Shouyaogangrou chapter of the Lingshu. Conclusions : The Yin and Yang of the needling sites from the Yin-Yang needling method in the Shouyaogangrou chapter of the Lingshu refers to the Yin and Yang meridians, or the Yin and Yang domains. In the context of disease site description, the former Yin and Yang describes a spatial aspect, while the latter Yin and Yang refers to the vicissitudes of qi according to temporal change.

• Journal of Oriental Neuropsychiatry
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• v.33 no.4
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• pp.389-400
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• 2022

Objectives: Depression is a highly prevalent disease, and the market for antidepressant drugs continues to grow at a steady rate. Although current antidepressants are reported to be effective, because of their low remission rate and side effects, new antidepressants are needed. The use of Banhahubak-tang (BHT) to treat the symptoms of depression is supported by experimental evidence. The proposed study will evaluate the efficacy and safety of BHT in treating depression. Methods: A randomized, waitlist-controlled, parallel clinical trial will be conducted to assess the efficacy of BHT in depression. A total of 84 participants with depression will be randomized into the intervention group or waitlist-control group at a 1:1 ratio. Patients in the intervention group will be administered BHT three times a day for four weeks and followed up for four more weeks after therapy completion. Patients in the waitlist-control group will undergo the same intervention and follow-up after a four-week waiting period. The primary outcome is change in the Korean version of the Hamilton Depression Rating Scale (K-HDRS) scores for major depressive disorders after four weeks. The secondary outcomes include scores on the K-HDRS, Korean Symptom Check List 95 (KSCL-95), Insomnia Severity Index (ISI), State-Trait Anxiety Inventory-Korean version (STAI-K), State-Trait Anger Expression Inventory-Korean version (STAXI-K), and the Instrument on Pattern Identifications for Depression and EuroQol-5 Dimension (EQ-5D). Conclusions: This trial will provide high-quality clinical evidence of the efficacy and safety of BHT in the management of depression.