Browse > Article
http://dx.doi.org/10.3347/kjp.2019.57.3.217

Cytopathic Change and Inflammatory Response of Human Corneal Epithelial Cells Induced by Acanthamoeba castellanii Trophozoites and Cysts  

Sohn, Hae-Jin (Department of Microbiology, Ajou University School of medicine, and Department of Biomedical Science, Graduate School of Ajou University)
Seo, Ga-Eun (Department of Microbiology, Ajou University School of medicine, and Department of Biomedical Science, Graduate School of Ajou University)
Lee, Jae-Ho (Department of Microbiology, Ajou University School of medicine, and Department of Biomedical Science, Graduate School of Ajou University)
Ham, A-Jeong (Department of Microbiology, Ajou University School of medicine, and Department of Biomedical Science, Graduate School of Ajou University)
Oh, Young-Hwan (Department of Microbiology, Ajou University School of medicine, and Department of Biomedical Science, Graduate School of Ajou University)
Kang, Heekyoung (Department of Microbiology, Ajou University School of medicine, and Department of Biomedical Science, Graduate School of Ajou University)
Shin, Ho-Joon (Department of Microbiology, Ajou University School of medicine, and Department of Biomedical Science, Graduate School of Ajou University)
Publication Information
Parasites, Hosts and Diseases / v.57, no.3, 2019 , pp. 217-223 More about this Journal
Abstract
Acanthamoeba castellanii has ubiquitous distribution and causes primary acanthamoebic keratitis (AK). AK is a common disease in contact lens wearers and results in permanent visual impairment or blindness. In this study, we observed the cytopathic effect, in vitro cytotoxicity, and secretion pattern of cytokines in human corneal epithelial cells (HCECs) induced by A. castellanii trophozoites and/or cysts. Morphological observation revealed that panked dendritic HCECs co-cultured with amoeba cysts had changed into round shape and gradually died. Such changes were more severe in co-culture with cyst than those of co-cultivation with trophozoites. In vitro cytotoxicity assay revealed the highest cytotoxicity to HCECs in the co-culture system with amoeba cysts. A. castellanii induced the expression of $IL-1{\alpha}$, IL-6, IL-8, and CXCL1 in HCECs. Secreted levels of $IL-1{\alpha}$, IL-6, and IL-8 in HCECs co-cultured with both trophozoites and cysts were increased at an early incubation time (3 and 6 hr). These results suggested that cytopathic changes and pro-inflammatory cytokines release of HCECs in response to A. castellanii, especially amoebic cysts, are an important mechanism for AK development.
Keywords
Acanthamoeba castellanii; acanthamoebic keratitis; human corneal epithelial cell; cytotoxicity; pro-inflammatory cytokine;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Walochnik J, Scheikl U, Haller-Schober EM. Twenty years of acanthamoeba diagnostics in Austria. J Eukaryot Microbiol 2015; 62: 3-11.   DOI
2 Hiti K, Walochnik J, Haller-Schober EM, Faschinger C, Aspock H. Viability of Acanthamoeba after exposure to a multipurpose disinfecting contact lens solution and two hydrogen peroxide systems. Br J Ophthalmol 2002; 86: 144-146.   DOI
3 Zanetti S, Fiori PL, Pinna A, Usai S, Carta F, Fadda G. Susceptibility of Acanthamoeba castellanii to contact lens disinfecting solutions. Antimicrob Agents Chemother 1995; 39: 1596-1598.   DOI
4 Seal DV. Acanthamoeba keratitis update-incidence, molecular epidemiology and new drugs for treatment. Eye (Lond) 2003; 17: 893-905.   DOI
5 Hurt M, Niederkorn J, Alizadeh H. Effects of mannose on Acanthamoeba castellanii proliferation and cytolytic ability to corneal epithelial cells. Invest Ophthalmol Vis Sci 2003; 44: 3424-3431.   DOI
6 Hurt M, Apte S, Leher H, Howard K, Niederkorn J, Alizadeh H. Exacerbation of Acanthamoeba keratitis in animals treated with anti-macrophage inflammatory protein 2 or antineutrophil antibodies. Infect Immun 2001; 69: 2988-2995.   DOI
7 van Klink F, Alizadeh H, He Y, Mellon JA, Silvany RE, McCulley JP, Niederkorn JY. The role of contact lenses, trauma, and Langerhans cells in a Chinese hamster model of Acanthamoeba keratitis. Invest Ophthalmol Vis Sci 1993; 34: 1937-1944.
8 Visvesvara GS, Balamuth W. Comparative studies on related free-living and pathogenic amebae with special reference to Acanthamoeba. J Protozool 1975; 22: 245-256.   DOI
9 Sohn HJ, Kang H, Seo GE, Kim JH, Jung SY, Shin HJ. Efficient Liquid Media for Encystation of Pathogenic Free-Living Amoebae. Korean J Parasitol 2017; 55: 233-238.   DOI
10 Moon EK, Xuan YH, Chung DI, Hong Y, Kong HH. Microarray analysis of differentially expressed genes between cysts and trophozoites of Acanthamoeba castellanii. Korean J Parasitol 2011; 49: 341-347.   DOI
11 Moon EK, Hong Y, Lee HA, Quan FS, Kong HH. DNA methylation of gene expression in Acanthamoeba castellanii encystation. Korean J Parasitol 2017; 55: 115-120.   DOI
12 Lyons TB, Kapur R. Limax amoebae in public swimming pools of albany, schenectady, and rensselaer counties, new york: their concentration, correlations, and significance. Appl Environ Microbiol 1977; 33: 551-555.   DOI
13 Kim JH, Kim D, Shin HJ. Contact-independent cell death of human microglial cells due to pathogenic Naegleria fowleri trophozoites. Korean J Parasitol 2008; 46: 217-221.   DOI
14 Brown TJ, Cursons RT, Keys EA. Amoebae from antarctic soil and water. Appl Environ Microbiol 1982; 44: 491-493.   DOI
15 Cerva L, Serbus C, Skocil V. Isolation of limax amoebae from the nasal mucosa of man. Folia Parasitol (Praha) 1973; 20: 97-103.
16 Paszko-Kolva C, Yamamoto H, Shahamat M, Sawyer TK, Morris G, Colwell RR. Isolation of amoebae and Pseudomonas and Legionella spp. from eyewash stations. Appl Environ Microbiol 1991; 57: 163-167.   DOI
17 Niederkorn JY, Alizadeh H, Leher H, McCulley JP. The pathogenesis of Acanthamoeba keratitis. Microbes Infect 1999; 1: 437-443.   DOI
18 Claerhout I, Kestelyn P. Acanthamoeba keratitis: a review. Bull Soc Belge Ophtalmol 1999; 274: 71-82.
19 Alizadeh H, Pidherney MS, McCulley JP, Niederkorn JY. Apoptosis as a mechanism of cytolysis of tumor cells by a pathogenic free-living amoeba. Infect Immun 1994; 62: 1298-1303.   DOI
20 Shin HJ, Cho MS, Kim HI, Lee M, Park S, Sohn S, Im KI. Apoptosis of primary-culture rat microglial cells induced by pathogenic Acanthamoeba spp. Clin Diagn Lab Immunol 2000; 7: 510-514.   DOI
21 Kurpakus-Wheater M, Kernacki KA, Hazlett LD. Maintaining corneal integrity how the "window" stays clear. Prog Histochem Cytochem 2001; 36: 185-259.
22 Kernacki KA, Barrett RP, Hobden JA, Hazlett LD. Macrophage inflammatory protein-2 is a mediator of polymorphonuclear neutrophil influx in ocular bacterial infection. J Immunol 2000; 164: 1037-1045.   DOI
23 Auran JD, Starr MB, Jakobiec FA. Acanthamoeba keratitis. A review of the literature. Cornea 1987; 6: 2-26.   DOI
24 Schuster FL, Visvesvara GS. Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals. Int J Parasitol 2004; 34: 1001-1027.   DOI
25 Marciano-Cabral F, Cabral G. Acanthamoeba spp. as agents of disease in humans. Clin Microbiol Rev 2003; 16: 273-307.   DOI
26 Im K, Kim DS. Acanthamoebiasis in Korea: two new cases with clinical cases review. Yonsei Med J 1998; 39: 478-484.   DOI
27 van Klink F, Alizadeh H, Stewart GL, Pidherney MS, Silvany RE, He Y, McCulley JP, Niederkorn JY. Characterization and pathogenic potential of a soil isolate and an ocular isolate of Acanthamoeba castellanii in relation to Acanthamoeba keratitis. Curr Eye Res 1992; 11: 1207-1220.   DOI
28 Visvesvara GS, Stehr-Green JK. Epidemiology of free-living ameba infections. J Protozool 1990; 37 (suppl): 25-33.   DOI
29 Visvesvara GS. Amebic meningoencephalitides and keratitis: challenges in diagnosis and treatment. Curr Opin Infect Dis 2010; 23: 590-594.   DOI