• Title/Summary/Keyword: Exposure energy

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NMR-based Metabolomic Responses of Zebrafish (Danio Rerio) by Fipronil Exposure

  • Lee, Sujin;Oh, Sangah;Kim, Seonghye;Lee, Wonho;Choi, Juyoung;Lee, Hani;Lee, Yujin;Kim, Suhkmann
    • Journal of the Korean Magnetic Resonance Society
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    • v.24 no.4
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    • pp.104-116
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    • 2020
  • Fipronil, the phenylpyrazole insecticide, is effective and used in various fields. Especially, fipronil was reliable because it was known to be specific on invertebrate animals than vertebrate animals including mammals. However, fipronil had potential risks that affect vertebrate animals as it blocks the gamma-aminobutyric acid (GABA) receptors that also exists in vertebrates as well as invertebrates. Therefore, it was necessary that harmful effects of fipronil on vertebrates are clarified. For this purpose, the zebrafish (Danio rerio) were used on behalf of vertebrate animals in present study. The zebrafish were exposed to 5 ㎍/L, 25 ㎍/L, and 50 ㎍/L of fipronil during 12, 24 and 72 hours. To closely observe toxic process, 12 hours and 24 hours of additional time point were set in the exposure test. Nuclear magnetic resonance (NMR)-based metabolomics is an approach to detect metabolic changes in organism resulted from external stimuli. In this study, NMR-based metabolomics showed the metabolic changes in zebrafish caused by fipronil exposure. Metabolic analysis revealed that fipronil interfered with energy metabolism and decreased the antioxidant ability in zebrafish. Antioxidant ability decline was remarkable at high exposure concentration. In addition, metabolic analysis results over time suggested that reactions for alleviating the excessive nerve excitation occurred in zebrafish after fipronil exposure. Through this study, it was elucidated that the adverse effects of fipronil on vertebrate animals are evident. The risk of fipronil on vertebrates can be no longer ignored. Moreover, this study has a meaning of practically necessary research for organism by examining the effects of fipronil at low concentrations existed in real environment.

Basic Physiological Research on the Wing Flapping of the Sweet Potato Hawkmoth Using Multimedia

  • Nakajima, Isao;Yagi, Yukako
    • Journal of Multimedia Information System
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    • v.7 no.2
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    • pp.189-196
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    • 2020
  • We have developed a device for recording biological data by inserting three electrodes and a needle with an angular velocity sensor into the moth for the purpose of measuring the electromyogram of the flapping and the corresponding lift force. With this measurement, it is possible to evaluate the moth-physiological function of moths, and the amount of pesticides that insects are exposed to (currently LD50-based standards), especially the amount of chronic low-concentration exposure, can be reduced the dose. We measured and recorded 2-channel electromyography (EMG) and angular velocity corresponding to pitch angle (pitch-like angle) associated with wing flapping for 100 sweet potato hawkmoths (50 females and 50 males) with the animals suspended and constrained in air. Overall, the angular velocity and amplitude of EMG signals demonstrated high correlation, with a correlation coefficient of R = 0.792. In contrast, the results of analysis performed on the peak-to-peak (PP) EMG intervals, which correspond to the RR intervals of ECG signals, indicated a correlation between ΔF fluctuation and angular velocity of R = 0.379. Thus, the accuracy of the regression curve was relatively poor. Using a DC amplification circuit without capacitive coupling as the EMG amplification circuit, we confirmed that the baseline changes at the gear change point of wing flapping. The following formula gives the lift provided by the wing: angular velocity × thoracic weight - air resistance - (eddy resistance due to turbulence). In future studies, we plan to attach a micro radio transmitter to the moths to gather data on potential energy, kinetic energy, and displacement during free flight for analysis. Such physiological functional evaluations of moths may alleviate damage to insect health due to repeated exposure to multiple agrochemicals and may lead to significant changes in the toxicity standards, which are currently based on LD50 values.

Gamma-ray Dose Measurements in a Human Phantom Using Thermoluminescent Dosimeter

  • Yoo, Young-Soo;Lee, Hyun-Duk
    • Nuclear Engineering and Technology
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    • v.6 no.4
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    • pp.239-247
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    • 1974
  • A human phantom of polyethylene has been designed and sculptured for studying the effective radiation safety control. The phantom has the approximate size of the Korean adult and was sliced into thirty-five transverse slabs, 2.5 cm thick, The relative dose at the specified position was determined from the exposure that a TLD badge worn on the surface of the phantom body received from external ${\gamma}$-ray. The variation of the exposure as a function of depth in the phantom was measured for uncollimated ${\gamma}$-ray using TLD rods, and also isodose curves were obtained for the anatomical cross-section of the critical organs of the body. To simulate radiation exposure condition in the nuclear facility, measurements were made for given angles of incident ${\gamma}$-ray. The front to back attenuation factor for human phantom of thickness 20 cm was 0.439 for Cs$^{137}$ ${\gamma}$-ray which is in reasonable agreement with the published data.

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A Probabilistic Safety Assessment of a Pyro-processed Waste Repository (A-KRS 처분 시스템 확률론적 안전성 평가)

  • Lee, Youn-Myoung;Jeong, Jongtae
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.10 no.4
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    • pp.263-272
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    • 2012
  • A GoldSim template program for a safety assessment of a hybrid-typed repository system, called A-KRS, in which two kinds of pyro-processed radioactive wastes, low-level metal wastes and ceramic high-level wastes that arise from the pyro-processing of PWR nuclear spent fuels are disposed of, has been developed. This program is ready both for a deterministic and probabilistic total system performance assessment which is able to evaluate nuclide release from the repository and farther transport into the geosphere and biosphere under various normal, disruptive natural and manmade events, and scenarios. The A-KRS has been probabilistically assessed with 9 selected input parameters, each of which has its own statistical distribution for a normal release and transport scenario associated with nuclide release and transport in and around the repository. Probabilistic dose exposure rates to the farming exposure group have been evaluated. A sensitivity of 9 selected parameters to the result has also been investigated to see which parameter is more sensitive and important to the exposure rates.

A Control Room Dose Assessment for a 1300 MWe PWR Following a Loss of Coolant Accident (냉각재(冷却材) 상실사고시(喪失事故時) 1300 MWe 급(級) PWR원전(原電) 주제어실(主制御室)의 선량평가(線量評價))

  • Chang, Si-Young;Ha, Chung-Woo
    • Journal of Radiation Protection and Research
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    • v.14 no.1
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    • pp.34-45
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    • 1989
  • The habitability of a reactor control room in a French 1300 MWe P'4 type PWR has been evaluated through the exposure dose assessment for the reactor operator following a Loss of Coolant Accident. The main hypotheses adopted in this evaluation are based on the French Standard Safety Analysis Report. A simple computer program, named COREX(Control Room EXposure), was developed to calculate : the time-integrated radioactivities released from the reactor building, the volume factors for radionuclides concerned and the resulting time-integrated external whole body and internal thyroid doses to the reactor operators staying in the control room up to 30 days following the LOCA. The results obtained in this study, on the whole, well agreed with those proposed by the EDF(Electricite de France) except for the case of the whole body exposure, which was attributed to the differences in the volume factors for the radionuclides concerned.

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A STUDY FOR THERMAL CONDUCTIVITY OF IRRADIATED CAVITY (와동내에 조사된 열의 전도양상에 관한 연구)

  • Baik, Byeong-Ju;Kim, Jae-Gon;Hur, Sun;Lee, Du-Cheol;Yun, Hyun-Du
    • Journal of the korean academy of Pediatric Dentistry
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    • v.25 no.3
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    • pp.583-597
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    • 1998
  • The purpose of this study was to evaluate temperature change occurred in enamel, dentin and pulp due to the heat from cavity prepration with laser. We made three models had different cavity depth: cavity depth of model A was 3.52mm, model B was 2.32mm, model C was 1.16mm. We irradiated cavity base with thermal capacity of $30J,100J,300J/cm^2s$ during few seconds and studied the change of temperature in tooth during 10 seconds, and estimated change of thermal capacity by different irradiated site and exposure time. At $300J/cm^2$ irradiation for 2 seconds, the temperature of irradiated surface was elevated fast according to irradiated thermal energy during 1 second. In proportion to continuous exposure time, temperature elevated slowly. The surface temperature was $1370^{\circ}C$. After discontinue of thermal irradiation, the heat of irradiated surface was diffused in dentin and pulp and the greatest temperature was made. The greatest temperature was disappeared within 10 seconds The greatest temperature of the inner part of model brought about very severe change by different depth. Temperature in pulp was raised by the greater irradiated energy density and exposure time.

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Vapor Exposure Effect of a Casting Solution on the Embedding and Radioactive Detection of CAYS in Double-layered Polysulffne Film (방사능탐지용 CAYS 함침 이중구조 폴리설폰막의 형상 및 특성에 제막공정의 습도가 미치는 영향)

  • Han Myeong-Jin;Nam Suk-Tae;Lee Kune-Woo;Seo Bum-Kyoung
    • Membrane Journal
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    • v.15 no.3
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    • pp.198-205
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    • 2005
  • Double-layered polymer films to assay the radioactive contamination were formulated using polysulfone (PSF) and cerium activated yttrium silicate (CAYS), consisting of a dense support layer and a CAYS-holding top layer prepared via the diffusion-induced phase inversion. As the vapor exposure process was omitted, the CAYS-holding layer showed a typical asymmetric structure, with CAYS being transfixed into the polymer network spread with large macropores. With the increase in vapor exposure time before immersion, morphology of the films transformed from asymmetric to sponge-like structures, with CAYS being localized in cellular structure. The border structure between the two layers reflects the phase inversion behavior of a cast solution during the coagulation. In the radioactive detection, the polymer phase in a film holding a sponge-like structure is so dense that the radionuclides, deposited on the film, could not filter through the phase, consequently resulting in the loss in the detection efficiency of the film.

The Characteristics of Bonding for Thermo-plastic using Solar Energy (태양에너지를 이용한 열경화성 플라스틱 접합특성)

  • Kim, Ok-Sam;Kim, Il-Soo;Son, Joon-Sik;Seo, Joo-Hwan;Moon, Chae-Joo
    • Transactions of the Korean Society of Machine Tool Engineers
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    • v.16 no.2
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    • pp.106-111
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    • 2007
  • In this research work attempts were made to study the bonding of thermo-plastics with adhesives using solar radiation. In order to study the curing behaviour necessary experiments were conducted under varying conditions of temperature, exposure time and power of solar energy. The cured samples were then studied under the optical microscope before subjecting to tensile testing in order to study their mechanical properties of thermo-plastics. The fracture surfaces were further studied under the Scanning Electron Microscopy(SEM) in order to research the microstructural changes that are taken place during curing. In order to measure the performance of solar energy cured joints the parameters such as; bond strength, surface morphology, the microstructual changes, variation in properties of adhesives bonded joints are compared to that of specimen cured at ambient conditions and specimen cured using microwave techniques.

A Study on Light-shelf system based on IT in Housing Space -Focus on Lighting Energy Saving- (주거공간을 위한 IT결합 광선반시스템 개발 연구 -조명에너지 저감을 중심으로-)

  • Ahn, Hye-Young;Lee, Heang-Woo;Kim, Yong-Seong
    • KIEAE Journal
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    • v.13 no.1
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    • pp.133-140
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    • 2013
  • The purpose of this study was to test the effectiveness of the light-shelf system combined with IT to save lighting energy in resident space. According to the multiple variables that affected intensity of illumination, various experiments were designed and conducted. The main findings of this study were following: (1)the optimal level of light-shelf system was formulated which met the appropriate range of intensity of illumination in living-room; (2)although the mixed light-shelf system indicated a high performance, it could often provide discomforts to residents according to the degree of exposure; (3)artificial lights using light-shelf system combined with IT showed a higher effectiveness than those using fixed light-shelf system displayed. This study provided initial information of light-shelf system formative processes that could contribute to the lighting energy diminution. Future studies should focus more on multiple approaches regarding different types of resident space planning.

PROTECTION OF DNA BY SCUTELLARlA BAICALENSIS IN HL-60 CELLS EXPOSED TO $\gamma$-RAYS; ANALYSED BY MICRONUCLEI FORMATION AND SINGLE CELL GELL ELECTROPHORESIS

  • Heon Oh;Park, Hae-Ran;Ham, Yeon-Ho;Kim, Sung-Ho;Jo, Sung-Kee
    • Proceedings of the Korean Society of Toxicology Conference
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    • 2001.10a
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    • pp.107-107
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    • 2001
  • In the present study, the protective effect of Scutellaria baicalensis against DNA damage in HL-60 cells exposed to $^{60}$ Co ${\gamma}$-rays was evaluated using micronuclei formation and alkaline single cell gel electrophoresis (SCGE, comet assay). The frequency of micronuclei was decreased in groups treated with water extract (P<0.01), polysaccaride fraction (P<0.01) and methanol fraction (P<0.01) before/after exposure to 200 cGy of ${\gamma}$-rays.(omitted)

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