• Asian Pacific Journal of Cancer Prevention
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• 제14권10호
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• pp.6069-6075
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• 2013

Background: At present, the diagnosis of colorectal cancer (CRC) requires a colorectal biopsy which is an invasive procedure. We undertook this pilot study to develop an alternative method and potential new biomarkers for diagnosis, and validated a set of well-integrated tools called ClinProt to investigate the serum peptidome in CRC patients. Methods: Fasting blood samples from 67 patients diagnosed with CRC by histological diagnosis, 55 patients diagnosed with colorectal adenoma by biopsy, and 65 healthy volunteers were collected. Division was into a model construction group and an external validation group randomly. The present work focused on serum proteomic analysis of model construction group by ClinProt Kit combined with mass spectrometry. This approach allowed construction of a peptide pattern able to differentiate the studied populations. An external validation group was used to verify the diagnostic capability of the peptidome pattern blindly. An immunoassay method was used to determine serum CEA of CRC and controls. Results: The results showed 59 differential peptide peaks in CRC, colorectal adenoma and health volunteers. A genetic algorithm was used to set up the classification models. Four of the identified peaks at m/z 797, 810, 4078 and 5343 were used to construct peptidome patterns, achieving an accuracy of 100% (> CEA, P<0.05). Furthermore, the peptidome patterns could differentiate the validation group with high accuracy close to 100%. Conclusions: Our results showed that proteomic analysis of serum with MALDI-TOF MS is a fast and reproducible approach, which may provide a novel approach to screening for CRC.

• Journal of Plant Biotechnology
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• 제5권1호
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• pp.13-17
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• 2003

Biotechnology is certainly one of the major landmarks in the 20th century history of science. It may produce enormous utility to human beings, but at the same time, it carries huge potential risks to the environment and public health. Thus, with a view to securing safety for the environment and public health in relation to the development and use of living modified organisms (LMOs), the Cartagena Protocol on Biosafety was adopted, in which a regulation Procedure as to the transboundary movements, transportation, handling, and use of LMOs were drawn up. In order to prepare for the entry into force of the Protocol, the Republic of Korea legislated the "Act on the Transboundary Movements of living modified organisms(hereinafter referred as the 'Act')" in Mar 2001, and has pre-announced the enactments of the enforcement ordinance and the enforcement regulation to the Act. Pursuant to the Act, the Ministry of Commerce, Industry and Energy, as a Competent National Authority, is making efforts to implement domestic biosafety schemes in cooperation with other bio-related government ministries. In order for these efforts to reap fruits, industry, academia, and research institutions should cooperate with one another, and civic groups and NGOs should narrow the differences in opinions and timely respond to the fast-changing situations. Focusing on the precautionary principle, the Protocol puts a great emphasis on the importance of information sharing amongst countries, and the Act also follows this principle. In order to gurantee biosafety, countries around the world, including the ROK, agreed to establish National Biosafety Clearing, designed to provide the information on the export/import of LMOs, R'||'&'||'Ds, risk assessment, safety control, etc. and register it on the CBD Central Biosafety Clearing House.e.

• 디지털융복합연구
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• 제14권3호
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• pp.219-226
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• 2016

본 연구에서는 유럽의 제조업 혁신의 발원지인 독일의 제조업 혁신 추진 전략을 살펴봄으로써 한국이 추진하고 있는 제조업 3.0을 성공적으로 추진하기 위한 전략적 함의를 제시하고자 한다. 현재 한국 경제의 잠재성장력은 점점 약화되어 왔으며, 신흥국과의 기술격차가 점차 감소되고 있어, 국가 경제의 경쟁력 제고를 위한 국가적인 노력이 필요한 상황이다. 신흥국의 성장은 제조업의 수출수요를 유발하여 새로운 기회 요인이 되고 있지만, 시장점유율을 놓고 후발개도국과 치열한 경쟁을 벌여야 하는 한국에게는 위협요인이기도 하다. 유사하게 유럽의 장기적인 경기침체와 신흥국의 부상에도 불구하고 여전히 높은 수준의 산업경쟁력을 유지하고 있는 독일은 바로 이러한 점에서 국내 제조업 발전 정책의 추진 및 개선에 시사점을 제공해 줄 것으로 사료된다.

• STI Policy Review
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• 제4권1호
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• pp.27-53
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• 2013

Technology research and development (R&D) expenditures have increased as most countries recognize that technological innovation is a significant factor for continued economic growth. R&D subsidies by governmental entities were permitted in accordance with the Subsidy and Countervailing Measure (SCM) Agreement under the World Trade Organization (WTO) system. However, according to Article 31 of the SCM Agreement the provision for R&D subsidies have been terminated as of January 2000 and legal disputes over R&D subsidies are likely to increase. The aircraft industry has been the only industry where R&D subsidies have become an issue under the WTO. This paper examines international trade disputes within the aircraft industry in regards to measures by Canada and bilateral disputes between the U.S. and the European Communities (EC). In these cases, various R&D subsidies on civil aircraft are found to be inconsistent with WTO rules. This study summarizes the WTO decisions on various R&D subsidies disputed in the aircraft cases and examines the type of R&D subsidies found to be inconsistent (or consistent) with the WTO to provide guidelines for current and future R&D subsidy policies in high-tech industries. The Canada-Aircraft case indicates that R&D subsidies directly targeted towards near market R&D projects with a high export potential will likely be in violation of current WTO rules. Furthermore, findings from the EC-Aircraft and the U.S.-Aircraft cases suggest that the forms (or the methods) of R&D subsidy distribution were not a sufficient condition for the WTO ruling; instead, what ultimately mattered was whether and specifically to whom the benefits of the R&D subsidies are conferred by the government entities.

• 한국물환경학회지
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• 제33권5호
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• pp.546-555
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• 2017

The Nutrient budget is one of the agricultural-environment indicators of OECD. A nutrient budget measures the surplus as the differential between the inputs and the outputs of within a certain boundary and within a specified period of time (i.e. one year). According to OECD, the annual nitrogen budget for Korea was $245kg\;N\;ha^{-1}$ in 2014, which corresponds to the first position among OECD countries. In Korea in 2014, about 90 % of livestock excreta was composted as solid and liquid manure, which are usually and customarily spread on agricultural land. The objectives of this study are intended to suggest methodology of the regional nitrogen budget as a nitrogen management tool, which considers conversion from raw excreta to composted manures based on the methodology of OECD/Eurostat, and application of the new method in an agricultural region of Korea. As a result, the calculated excess rate of hydrospheric nitrogen surplus was $251kg\;N\;ha^{-1}$ (in the region in 2014), which indicates the presence of potential risks emanating from excessive nitrogen, with regard to both export water and soil environments. The findings also assert that this was shown to be one of the most important elements in the nitrogen budget, which translates to the actual amounts of nitrogen lost during the solid composting process. To better understand the process and the reliability of the method, it is necessary to analyze the sensitivity of the relevant co-efficients used in the method in the near future.

• Journal of Microbiology and Biotechnology
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• 제22권6호
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• pp.742-753
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• 2012

Copper-containing compounds are introduced into the environment through agricultural chemicals, mining, and metal industries and cause severe detrimental effects on ecosystems. Certain microorganisms exposed to these stressors exhibit molecular mechanisms to maintain intracellular copper homeostasis and avoid toxicity. We have previously reported that the soil bacterial isolate Achromobacter sp. AO22 is multi-heavy metal tolerant and exhibits a mer operon associated with a Tn21 type transposon. The present study reports that AO22 also hosts a unique cop locus encoding copper homeostasis determinants. The putative cop genes were amplified from the strain AO22 using degenerate primers based on reported cop and pco sequences, and a constructed 10,552 base pair contig (GenBank Accession No. GU929214). BLAST analyses of the sequence revealed a unique cop locus of 10 complete open reading frames, designated copSRABGOFCDK, with unusual separation of copCD from copAB. The promoter areas exhibit two putative cop boxes, and copRS appear to be transcribed divergently from other genes. The putative protein CopA may be a copper oxidase involved in export to the periplasm, CopB is likely extracytoplasmic, CopC may be periplasmic, CopD is cytoplasmic/inner membrane, CopF is a P-type ATPase, and CopG, CopO, and CopK are likely copper chaperones. CopA, B, C, and D exhibit several potential copper ligands and CopS and CopR exhibit features of two-component regulatory systems. Sequences flanking indicate the AO22 cop locus may be present within a genomic island. Achromobacter sp. strain AO22 is thus an ideal candidate for understanding copper homeostasis mechanisms and exploiting them for copper biosensor or biosorption systems.

• 한국빅데이터학회지
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• 제3권1호
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• pp.21-32
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• 2018

현재 국내에서 서비스되고 있는 웹툰은 네이버, 다음 등 포탈을 중심으로 매우 빠른 성장을 보이고 있으며, 잠재적 시장성이 매우 높은 산업으로 촉망받고 있다. 웹툰의 특성 가장 두드러지는 특징은 다양한 요구를 가진 고객층을 대상으로 서비스를 제공할 수 있는 것이다. 이 때문에 국내뿐만 아니라 해외에서도 웹툰산업을 긍정적으로 판단하고 관련 산업 시장을 확충하고 있다. 이러한 현실은 국내 웹툰 작가 및 사업자로 하여금 웹툰을 기존의 k-pop이나 드라마 중심의 한류 컨텐츠와 더불어 새로운 한류 컨텐츠로 수출할 수 있는 기회를 제공한다. 성공적인 시장진출을 위해서는 수출 대상 국가의 소비자층에 대한 면밀한 분석이 요구된다. 본 논문에서는 네이버 웹툰에서 수집한 데이터의 분석을 통해 각 국가에서 서비스되는 웹툰과 해당 웹툰을 구독하는 소비자층에 대한 특성을 도출해보았다. 이를 통해 국가별로 웹툰 구독자의 선호도에 영향을 미치는 요소들을 빠르게 파악할 수 있을 것이다.

• 국제지역연구
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• 제17권4호
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• pp.1-21
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• 2013

본 논문에서는 ASEAN의 10개 회원국, 한국, 중국, 일본, 호주, 뉴질랜드, 인도 등 16개국으로 구성된 역내포괄적경제동반자협정(RCEP)가 RCEP의 회원국 경제와 전세계 경제에 어떠한 영향을 미치게 될 것인가를 다(多)지역 다(多)산업 연산일반균형(CGE)모형을 이용하여 정량적으로 분석하였다. Baseline 시나리오를 바탕으로 RCEP 회원국 구성의 특성에 입각하여 단계별 3가지 정책시나리오, 즉 한-중-일FTA(시나리오 1), ASEAN+3 FTA(시나리오 2), RCEP(시나리오 3)를 설정하였다. 3가지 정책 시나리오의 영향을 실질GDP, 후생수준의 지표로서 등가변환, 수출 수입물량, 국제수지, 교역조건 등 거시경제적 변수에 미치는 영향으로 제시하였다. 본 연구의 주요 결과를 보면, RCEP를 통해 모든 RCEP 회원국의 실질GDP가 증가할 것으로 전망되며 특히 한국의 실질GDP는 모든 RCEP회원국 중 가장 많이(2.43%) 증가할 것으로 예측되었다. 이는 한국이 RCEP 추진에 보다 적극적인 역할을 수행할 만한 경제적 이점이 있음을 시사하는 것이라고 할 수 있다.

• 한국버섯학회지
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• 제18권4호
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• pp.292-296
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• 2020

본 연구는 한라산천연보호구역의 기초자료 확보를 목적으로 수행된 한라산천연보호구역 기초학술조사 중 버섯류에 대한 연구로, 2018년 4월부터 10월까지 7개월 동안 성판악탐방로와 돈내코탐방로 사이 지역을 대상으로 수행되었다. 조사된 버섯은 총 65과 184속 392종이며, 이 중 187종의 버섯이 죽은 나무나 나뭇가지에서 발생하였다. 주름버섯목에 속하는 버섯이 184종으로 가장 높게 나타났고, 시기적으로는 7월에 206종으로 가장 많이 발생하였다. 또한 대부분의 버섯은 600~800 m 사이 고도에서 발생하였다. 본 연구에서 조사된 버섯들 중 기후변화 생물지표종에 해당되는 버섯이 5종, 후보종 2종이 나타났으며, 392종 중 국외 반출 승인 대상 생물자원에는 163종이 해당되었다(*Appendix 홈페이지에 업로드).

• 한국축산식품학회지
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• 제42권1호
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• pp.61-72
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• 2022

Antioxidants present in fruits and vegetables have a potential to reduce disease risk, and increase the shelf life of food products by reducing lipid oxidation. The effect of marination with antioxidants-rich fruit juices on quality characteristics of vacuum-packed chicken wings were examined during frozen storage. Chicken wings were mixed separately with marinades containing pineapple juice, June plum juice, and mango juice and kept for 12 h and 24 h. Three best marination conditions were selected based on a sensory evaluation. Antioxidant activity and total phenolic content of fruit juices, and marinade uptake, and marinade loss of marinated chicken wings were determined. In addition, vacuum packed marinated chicken wings were tested for pH, water holding capacity (WHC), 2-thiobarbituric acid reactive substances (TBARS) value and antioxidant activity over a 4-wk frozen storage. The best sensory properties were reported from chicken wings marinated with pineapple juice for 24 h, mango juice for 24 h, and June plum juice for 12 h (p<0.05) compared to other marinade-time combinations. Mango juice showed the highest antioxidant activity (92.2%) and total phenolic content (38.45 ㎍/mL; p<0.05) compared to other fruit juices. The pH and WHC of vacuumpacked chicken wings were slightly decreased over the frozen storage (p<0.05). Moreover, chicken wings marinated with mango juice had the lowest TBARS values and the highest 2,2-diphenyl-1-picryl-hydrazyl-hydrate free radical scavenging activity. In conclusion, mango juice was selected among tested as the most effective marinade for enhancing the oxidative stability of lipid while maintaining the other meat quality traits of vacuum-packed chicken wings.