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Dietary protease improves growth performance, nutrient digestibility, and intestinal morphology of weaned pigs

  • Park, Sangwoo;Lee, Jeong Jae;Yang, Boung Mo;Cho, Jin Ho;Kim, Soyun;Kang, Joowon;Oh, Sejong;Park, Dong-Jun;Perez-Maldonado, Rider;Cho, Jee-Yeon;Park, Il-Hun;Kim, Hyeun Bum;Song, Minho
    • Journal of Animal Science and Technology
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    • v.62 no.1
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    • pp.21-30
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    • 2020
  • This study was conducted to evaluate effects of dietary protease (PR) on growth performance, nutrient digestibility, and intestinal morphology of weaned pigs. A total of 75 weaned pigs [7.06 ± 0.18 kg of average body weight (BW); 28 day old] were randomly allotted to 3 dietary treatments in a randomized complete block design (blocks = BW and sex): a diet based on corn and soybean meal to meet the requirement of crude protein (CP) as a positive control (PC; CP = 24.49%), a low protein diet as a negative control (NC; CP = 22.51%), and NC + 0.02% PR. The PR used in this study was a commercial product containing 75,000 protease units/g derived from Nocardiopsis prasina produced in Bacillus licheniformis. Pigs were fed the dietary treatments for 6 weeks and the diets containing 0.2% chromic oxide for the last week of this study. Blood, feces, ileal digesta, and ileum samples were collected from randomly selected two pigs in each pen on respective time points. Measurements were growth performance, apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of dry matter (DM), CP, and energy, frequency of diarrhea, packed cell volume (PCV), and ileal morphology of weaned pigs. Pigs fed PC and PR had higher (p < 0.05) final BW, average daily gain (ADG), and gain to feed ratio (G:F) during overall experimental period than those fed NC. Pigs fed PC and PR had higher (p < 0.05) AID or ATTD of DM, CP, or energy than those fed NC. Moreover, pigs fed PR had higher ratio between villus height and crypt depth (p < 0.05) and number of goblet cells (p < 0.05) than those fed NC. Addition of PR decreased (p < 0.05) frequency of diarrhea for the first two weeks after weaning compared with PC and NC. In addition, pigs fed PR had lower (p < 0.05) PCV on d 14 after weaning than those fed PC and NC. In conclusion, addition of PR in nursery diets with a low protein level significantly improved growth performance, nutrient digestibility, and intestinal morphology of weaned pigs.

The Ammonia Removal Capacity of a Few Kinds of Filter Media in a Water Reuse quaculture System (순환 여과식 양식 시설에 이용될 수종의 여과 재료의 효능에 관한 연구)

  • KIM In-Bae;KIM Pyong-Kih;CHEE Young-Ok
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.20 no.6
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    • pp.561-567
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    • 1987
  • The purpose of this study was to find out the removal capacity of harmful ammonia by different filter media in the submerged biological filters in a given space of chamber. Four materials, pile cloth, corrugated skylight roofing plate, embossed plastic plate, and gravel, were used as the experimental filter media. Each filter medium was placed in two aquariums, each aquarium measuring $90cm\times60cm\times60cm\;(depth)$. Under the normal operating condition, the average of mean ammonia removal rates during the first and second functioning periods by each filter material which occupied tile space in the filter chamber (aquarium) was as follows: 1. Pile cloth: $8.381\;g{\cdot}m^{-3}.\;day^{-1}$ 2. Corrugated skylight roofing plate: $7.834\;g{\cdot}m^{-3}.\;day^{-1}$ 3. Embossed plastic plate: $7.797\;g{\cdot}m^{-3}.\;day^{-1}$ 4. Gravel: $7.051\;g{\cdot}m^{-3}.\;day^{-1}$ Thus, there were no significant differences between the media, but at the time of practical application of these materials, some other factors such as investment cost, easiness for the removal of excess detritus accumulated in tile interstices of filter media, etc. should be fallen into consideration. When large units are required, in particular, removal of excess detritus from tile gravel bed is extremely difficult, and in case of pile cloth filters the installation work is much complicated and a problem in supporting the structure when drained also exists. In these respects, corrugated skylight roofing plate and embossed plastic plate seem to be more optimal, but again in practice the local situation for the availability and the price of the materials should be rechecked and the fitness of tile materials in the particular filter chambers under use or under consideration for construction must be taken into account.

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FLEXION EFFECTS OF HEAT TREATMENT AND POST-SOLDRING OF CERAMO-METAL FIXED PARTIAL DENTURE FRAMEWORKS USING HOLOGRAPHIC INTERFEROMETRY (Holographic Interferometry를 이용한 하악 구치부 도재소부 전장관용 금속 구조물의 굴곡성향에 대한 연구)

  • Choi, Jin-Woong;Woo, Yi-Hyung;Choi, Boo-Byung
    • The Journal of Korean Academy of Prosthodontics
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    • v.34 no.4
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    • pp.869-902
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    • 1996
  • Flexion of a metal/ceramic fixed partial denture(EPD) frameworks under function can cause fracture of porcelain or deterioration of the cement seal. This study evaluated the flexion characteristics of three-unit mandibular FPD frameworks, repacing the second pre-molar under compressive load(200g, 400g). Testing was accompished with real-time holographic interferometry, using 6 porcelain fused-to metal frameworks. Tested alloys were non-precious alloy(Heracles, Holland), semi-precious alloy(Degudent U, Germany) and precious alloy(Degudent H, Germany). Changes of the fringe patterns according to the heat treatment(porcelain firing cycle), various loads(200g, 400g), occlusal forms(occlusal porcelain veneering, facial porcelain veneering), various alloys and post-soldering units were compared. Dental study model(Nissan dental products, Inc. D51DP-500A, Japan) and six 3-unit metal/ceramic fixed partial denture frameworks were used as experimental materials. 36 holograms were taken on fixed dental study model by using the 10mW He-Ne laser and real-time holographic interferometry. On the basis of this study, the following conclusions can be drawn : 1. In the frameworks for facial porcelain veneering, the semi-precious alloy framework was least deformed and precious alloy framework, non-precious alloy framework orderly before heat treatment, and the deformation was not shown great difference among three alloys after heat treatment and post-soldering. 2. In the frameworks for occlusal porcelain veneering, the precious alloy framework was greatest deformed and the deformation was not difference between semi-precious alloy framework and non-precious alloy framework before, after heat treatment, and the deformation was not shown great difference among three alloys after post soldering. 3. In the non-precious alloy frameworks for facial porcelain veneering and occlusal porcelain veneering, the deformation was greatly decreased after heat treatment and conversely increased after post-soldering. 4. In the semi-precious alloy framework for facial porcelain veneering, the deformation was not detectable after heat treatment and increased after post-solder. And in the frame-work for occlusal porcelain veneering, the deformation was slightly decreased after heat treatment and increased after post-soldering. 5. In the precious alloy framework for facial porcelain veneering, the deformation was greatly decreased after heat treatment and increased after post-soldering, And in the framework for occlusal porcelain veneering, the deformation was greatly decreased after heat treatment and decreased after post-soldering.

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Immunohistochemical study on the atretic and the growing follicles after experimental superovulation in rats 2. Atresia and growing of follicles (과배란 유기된 rat 난소에 퇴축난포와 성장난포에 대한 면역조직화학적 연구 2. 동원된 난포의 퇴축과 성장에 대하여)

  • Kwak, Soo-dong;Koh, Phil-ok;Kim, Chong-sup
    • Korean Journal of Veterinary Research
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    • v.37 no.1
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    • pp.79-86
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    • 1997
  • This study was designed to investigate the effects of superovulation on the growing and mature follicles following gonadotrophin treatments in mature rat by immunohistochemical methods. Eighteen mature rats (Sprague-Duwely, 190~230gm) were randomly alloted into 3 groups. One group was control group, another FSH-treated group was injected intramuscularly with 0.5 units of follicular stimulating hormone(FSH) / rat, and third PMS and HCG-treated group was injected intramuscularly with 20~25IU of pregnant mare serum(PMS) / rat and then at the 48 hrs later, with 20~25IU of human chorionic gonadotropin(HCG) / rat. Half the number of rats were administrated intraperitoneally with bromodeoxyuridine(Brdur, 0.2mg/gm BW once) at 2 hours before exanguination and the remainder of rats were sacrified without Brdur administration. The investigation by immunohistochemical methods using paraffin sections of ovaries was performed by using anti-Brdur antibody and PCNA(proliferating cell nuclear antigen) antibody for labeling proliferating cells in follicles. In immunohistochemical findings, follicles squeezed by peripheral corpus luteum or follicles large follicles with loosly and irregularly distributed granulosa cells and although with compacted granulosa cells, middle follicles with dilated round or oval follicular antrum were confirmed as atretic follicles. The proportions of atretic follicles in control group were 29.8%, 21.7% and 14.2% respectivley at large, middle and small follicles and mean proportions of these all 3 grade follicles were 26.7%. The proportions of atretic follicles in FSH-treated group were 35.4%, 24.9% and 10.4% respectively at large, middle and small follicles and mean proportions of these all 3 grade follicles were 28.1%. The proportions of atretic follicles in PMS and HCG-treated group were 44.7%, 24.0% and 12.7% respectively at large, middle and small follicles, and mean proportions of these all 3 grade follicles were 29.7%. The above findings reveal that the group with higher proportion of atretic follicles were ordered as large, middle and small follicles in size, and these proportions were increased in hormone treated two groups with more number of more growing and mature follicles when compared with control group.

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Birth statistics of high birth weight infants (macrosomia) in Korea

  • Kang, Byung-Ho;Moon, Joo-Young;Chung, Sung-Hoon;Choi, Yong-Sung;Lee, Kyung-Suk;Chang, Ji-Young;Bae, Chong-Woo
    • Clinical and Experimental Pediatrics
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    • v.55 no.8
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    • pp.280-285
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    • 2012
  • Purpose: The authors analyzed the trend from the birth-related statistics of high birth weight infants (HBWIs) over 50 years in Korea from 1960 to 2010. Methods: We used 2 data sources, namely, the hospital units (1960's to 1990's) and Statistics Korea (1993 to 2010). The analyses include the incidence of HBWIs, birth weight distribution, sex ratio, and the relationship of HBWI to maternal age. Results: The hospital unit data indicated the incidence of HBWI as 3 to 7% in the 1960's and 1970's and 4 to 7% in the 1980's and 1990's. Data from Statistics Korea indicated the percentages of HBWIs among total live births decreased over the years: 6.7% (1993), 6.3% (1995), 5.1% (2000), 4.5% (2000), and 3.5% (2010). In HBWIs, the birth weight rages and percentage of incidence in infants' were 4.0 to 4.4 kg (90.3%), 4.5 to 4.9 kg (8.8%), 5.0 to 5.4 kg (0.8%), 5.5 to 5.9 kg (0.1%), and >6.0 kg (0.0%) in 2000 but were 92.2%, 7.2%, 0.6%, 0.0%, and 0.0% in 2009. The male to female ratio of HBWIs was 1.89 in 1993 and 1.84 in 2010. In 2010, the mother's age distribution correlated with low (4.9%), normal (91.0%), and high birth weights (3.6%): an increase in mother's age resulted in an increase in the frequency of low birth weight infants (LBWIs) and HBWIs. Conclusion: The incidence of HBWIs for the past 50 years has been dropping in Korea. The older the mother, the higher was the risk of a HBWI and LBWI. We hope that these findings would be utilized as basic data that will aid those managing HBWIs.

A Design of an AMI System Based on an Extended Home Network for the Smart Grid (스마트 그리드를 위한 확장 홈 네트워크 기반의 AMI 시스템 설계)

  • Hwang, Yu-Jin;Lee, Kwang-Hui
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.49 no.7
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    • pp.56-64
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    • 2012
  • A smart grid is the next generation power grid which combines the existing power grid with information technology, so an energy efficient power grid can be provided. In this paper, in order to build an efficient smart grid an AMI system, which gears with the existing home network and provides an user friendly management function, is proposed. The proposed AMI system, which is based on an extended home network, consists of various functional units; smart meters, communication modules, home gateway, security modules, meter data management modules (MDMM), electric power application modules and so on. The proposed home network system, which can reduce electric power consumption and transmit data more effectively, is designed by using IEEE 802.15.4. The extended home gateway can exchange energy consumption information with the outside management system via web services. The proposed AMI system is designed to enable two-way communication between the home gateway and MDMM via the Internet. The AES(Advanced Encryption Standard) algorithm, which is a symmetric block cipher algorithm, is used to ensure secure information exchange. Even though the results in this study could be limited to our experimental environment, the result of the simulation test shows that the proposed system reduces electric power consumption by 4~42% on average compared to the case of using no control.

Channel characteristics of multi-path power line using a contactless inductive coupling unit (비접촉식 유도성 결합기를 이용한 다중경로 전력선 채널 특성)

  • Kim, Hyun-Sik;Sohn, Kyung-Rak
    • Journal of Advanced Marine Engineering and Technology
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    • v.40 no.9
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    • pp.799-804
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    • 2016
  • Broadband powerline communication (BPLC) uses distribution lines as a medium for achieving effective bidirectional data communication along with electric current flow. As the material characteristics of power lines are not good at the communication channel, the development of power line communication (PLC) systems for internet, voice, and data services requires measurement-based models of the transfer characteristics of the network suitable for performance analysis by simulation. In this paper, an analytic model describing a complex transfer function is presented to obtain the attenuation and path parameters for a multipath power line model. The calculated results demonstrated frequency-selective fading in multipath channels and signal attenuation with frequency, and were in good agreement with the experimental results. Inductive coupling units are used as couplers for coupling the signal to the power line to avoid physical connections to the distribution line. The inductance of the ferrite core, which depends on the frequency, determines the cut-off frequency of the inductive coupler. Coupling loss can be minimized by increasing the number of windings around the coupler. Coupling efficiency was improved by more than 6 dB with three windings compared to the results obtained with one winding.

Effect of Temperature at Different Performance Stages of Aphelinus varipes (Hym. Aphelinidae) Parasitizing the Green Peach Aphid, Myzus persicae (복숭아혹진딧물에 기생하는 목화면충좀벌의 발육단계별 온도영향)

  • Choi, Yong Seok;Hwang, In Su;Park, Deog Gee;Choe, Kwang Ryul
    • Korean journal of applied entomology
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    • v.51 no.4
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    • pp.343-348
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    • 2012
  • Developmental period, mummification, pupal mortality, and sex ratio of Korean strain of Aphelinus varipes F$\ddot{o}$rster parasitizing the green peach aphid, Myzus persicae were studied at 20, 25, 30, and $35^{\circ}C$ in controlled climate cabinets. The plastic container with a leaf disc of sweet pepper (Capsicum annuum L.) was used as experimental units. Green peach aphids at different developmental stages were reared in four different temperatures and presented to A. varipes for 12 hours. A female adult of A. varipes was allowed to parasitize for 12 hours in each plastic petri dish. The periods from egg to adult of A. varipes were 18.3 days, 14.7 days, 10.4 days, and 9.3 days at $20^{\circ}C$, $25^{\circ}C$, $30^{\circ}C$, and $35^{\circ}C$, respectively. The pupal mortalities of A. varipes were 0%, 1.5%, 2.8%, and 10.2% at $20^{\circ}C$, $25^{\circ}C$, $30^{\circ}C$, and $35^{\circ}C$, respectively. The mummifications of A. varipes were significantly different in all developmental stages of the aphid. The mummification of A. varipes parasitizing green peach aphid was the highest at 1st and 2nd in $20^{\circ}C$ and $25^{\circ}C$, but the highest at 3rd (84.4-85.0%) in $30^{\circ}C$ and $35^{\circ}C$. The host feedings of A. varipes were $20^{\circ}C$ (12.5%), $25^{\circ}C$ (17.3%), $30^{\circ}C$ (16%), and $35^{\circ}C$ (10.9%), respectively. The sex ratios of A. varipes female were $20^{\circ}C$ (49%), $25^{\circ}C$ (46%), $30^{\circ}C$ (69%), and $35^{\circ}C$ (60%), respectively.

A Comic Facial Expression Method for Intelligent Avatar Communications in the Internet Cyberspace (인터넷 가상공간에서 지적 아바타 통신을 위한 코믹한 얼굴 표정의 생성법)

  • 이용후;김상운;청목유직
    • Journal of the Institute of Electronics Engineers of Korea CI
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    • v.40 no.1
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    • pp.59-73
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    • 2003
  • As a means of overcoming the linguistic barrier between different languages in the Internet, a new sign-language communication system with CG animation techniques has been developed and proposed. In the system, the joint angles of the arms and the hands corresponding to the gesture as a non-verbal communication tool have been considered. The emotional expression, however, could as play also an important role in communicating each other. Especially, a comic expression is more efficient than real facial expression, and the movements of the cheeks and the jaws are more important AU's than those of the eyebrow, eye, mouth etc. Therefore, in this paper, we designed a 3D emotion editor using 2D model, and we extract AU's (called as PAU, here) which play a principal function in expressing emotions. We also proposed a method of generating the universal emotional expression with Avatar models which have different vertex structures. Here, we employed a method of dynamically adjusting the AU movements according to emotional intensities. The proposed system is implemented with Visual C++ and Open Inventor on windows platforms. Experimental results show a possibility that the system could be used as a non-verbal communication means to overcome the linguistic barrier.

Parallel Inverse Transform and Small-sized Inverse Quantization Architectures Design of H.264/AVC Decoder (H.264/AVC 복호기의 병렬 역변환 구조 및 저면적 역양자화 구조 설계)

  • Jung, Hong-Kyun;Cha, Ki-Jong;Park, Seung-Yong;Kim, Jin-Young;Ryoo, Kwang-Ki
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2011.10a
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    • pp.444-447
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    • 2011
  • In this paper, parallel IT(inverse transform) architecture and IQ(inverse quantization) architecture with common operation unit for the H.264/AVC decoder are proposed. By using common operation unit, the area cost and computational complexity of IQ are reduced. In order to take four execution cycles to perform IT, the proposed IT architecture has parallel architecture with one horizontal DCT unit and four vertical DCT units. Furthermore, the execution cycles of the proposed architecture is reduced to five cycles by applying two state pipeline architecture. The proposed architecture is implemented to a single chip by using Magnachip 0.18um CMOS technology. The gate count of the proposed architecture is 14.3k at clock frequency of 13MHz and the area of proposed IQ is reduced 39.6% compared with the previous one. The experimental result shows that execution cycle the proposed architecture is about 49.09% higher than that of the previous one.

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