• Title/Summary/Keyword: Experimental Units

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Comparison of apical extrusion of intracanal bacteria by various glide-path establishing systems: an in vitro study

  • Dagna, Alberto;El Abed, Rashid;Hussain, Sameeha;Abu-Tahun, Ibrahim H;Visai, Livia;Bertoglio, Federico;Bosco, Floriana;Beltrami, Riccardo;Poggio, Claudio;Kim, Hyeon-Cheol
    • Restorative Dentistry and Endodontics
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    • v.42 no.4
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    • pp.316-323
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    • 2017
  • Objectives: This study compared the amount of apically extruded bacteria during the glide-path preparation by using multi-file and single-file glide-path establishing nickel-titanium (NiTi) rotary systems. Materials and Methods: Sixty mandibular first molar teeth were used to prepare the test apparatus. They were decoronated, blocked into glass vials, sterilized in ethylene oxide gas, infected with a pure culture of Enterococcus faecalis, randomly assigned to 5 experimental groups, and then prepared using manual stainless-steel files (group KF) and glide-path establishing NiTi rotary files (group PF with PathFiles, group GF with G-Files, group PG with ProGlider, and group OG with One G). At the end of canal preparation, 0.01 mL NaCl solution was taken from the experimental vials. The suspension was plated on brain heart infusion agar and colonies of bacteria were counted, and the results were given as number of colony-forming units (CFU). Results: The manual instrumentation technique tested in group KF extruded the highest number of bacteria compared to the other 4 groups (p < 0.05). The 4 groups using rotary glide-path establishing instruments extruded similar amounts of bacteria. Conclusions: All glide-path establishment instrument systems tested caused a measurable apical extrusion of bacteria. The manual glide-path preparation showed the highest number of bacteria extruded compared to the other NiTi glide-path establishing instruments.

Influence of immediate loading on the removal torque value of mini-screws (교정력의 즉시 부하가 미니스크류의 제거 회전력에 미치는 영향의 평가)

  • Sun, Seung-Bum;Kang, Yoon-Goo;Kim, Seung-Hun;Mo, Sung-Seo;Kook, Yoon-Ah
    • The korean journal of orthodontics
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    • v.37 no.6
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    • pp.400-406
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    • 2007
  • The purpose of this experimental study was to evaluate the effect of immediate orthodontic loading on the stability at the bone-implant interface of titanium miniscrews in a rabbit model. Methods: Forty titanium miniscrews (1.6 mm diameter, 8 mm length) were inserted in the tibiae of 10 rabbits. Twenty test group miniscrews were subjected to continuous orthodontic forces of 200g immediately after implantation for a period of 6 weeks. The remaining 20 control group miniscrews were left unloaded for the same follow-up interval. Removal torque values were recorded using a digital torque gauge. An independent t-test was performed. Results: All the miniscrews were stable, and exhibited no mobility or displacement throughout the experimental period. Histologically, miniscrews were well-integrated into bone. No statistically significant differences in removal torque data were found between the loaded test and the unloaded control groups. Conclusions: These findings suggest that titanium miniscrews can be used as anchoring units for orthodontic tooth movement immediately after insertion.

Laboratory-Acquired Infections with Hantavirus at a Research Unit of Medical School in Seoul, 1996 (의과대학 연구동에서 발생한 유행성출혈열 역학조사)

  • Cho, Soo-Hun;Kang, Dae-Hee;Kim, Suhng-Gwon;Kim, Ik-Sang;Hong, Sung-Tae;Ju, Young-Su
    • Journal of Preventive Medicine and Public Health
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    • v.32 no.3
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    • pp.269-275
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    • 1999
  • Background : In April 6, 1990, a male researcher who has worked at a research unit at the Basic Research Building of Seoul National University(SNU) College of Medicine admitted to SNU Hospital due to persistent fever. He was diagnosed serologically as hemorrhagic fever with renal syndrome(HFRS). Another female researcher in the same unit was also diagnosed as HFRS at the same hospital several days later. Epidemic investigation of HFRS was conducted to determine the magnitude of the problems since these two cases were strongly suspected to have laboratory-acquired infections of HFRS. Methods : All researchers and employees working at the Basic Research Building(BRB) of SNU College of Medicine as of April 1, 1996 were recruited for the study, information on symptoms of HFRS and history of contact to experimental animals were collected by self-administered questionnaires and serological tests among study subjects were also conducted by indirect immunofluorescent antibody(IFA) to hantavirus. The experimental animals were also serologically tested for infection with hantavirus by IFA. Results : Among 218 surveyed, six researchers and an animal caretaker had hantavirus antibodies above 1:20 in IFA titer. Five of seven sero-positive subjects had antibodies above 1:640 in IFA titer and had shown clinical symptoms compatible to HFRS during Jan. 1 to Apr, 20, 1996. The sero-positive persons had handled animals more frequently than sero-negative persons (OR, 19,68; 95% Cl, 1.11-350.40) and handling animals at the animal quarter at School of Public Health(SPH) had shown consistently higher risk to get infected with hantavirus irrespective of types of animals handled (OR, 4.90-6.37). Sero-positivity of rats of the aniamal quarter at BRB was 30-60%, whereas 80% of rats at SPH tested were shown sero-positivity. Conclusion: There was a epidemic of HFRS in research units of a medical school during the period from Jan. through Apr, Further investigation is needed to determine the extent and the mode of transmission of the laboratory-acquired infection with hantavirus in other research facilities.

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Effect of Organic Soil Conditioner Ratio on the Soil Moisture Content and Growth of Cotoneater horizontalis in the Container Type for Wall-Planting under Non-irrigation (무관수 용기형 벽면녹화에서 유기질 토양개량제가 토양수분함량과 홍자단의 생육에 미치는 영향)

  • Ju, Jin-Hee;Lee, Sun-Young;Yoon, Yong-Han
    • Journal of Environmental Science International
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    • v.22 no.1
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    • pp.17-23
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    • 2013
  • In order to evaluate the effect of various organic soil conditioner ratios on the soil moisture content and growth of Cotoneaster horizontalis, a container type for wall-planting experiment was conducted in a greenhouse at Konkuk University under non-irrigation. The experimental planting grounds were prepared with different organic soil conditioner ratios ($A_1L_0$, $A_8L_1$, $A_4L_1$ $A_2L_1$ and $A_1L_1$), and a drought-tolerant ornamental variety of Cotoneaster horizontalis was planted. The change in soil moisture content, plant height, number of branches, number of dead leaves, number of leaves, number of shoots, length of node, length of leaf, width of leaf, root-collar caliper, chlorophyll content, and survival rate were investigated, from April to Jun 2010. The results of soil moisture content measurements were analyzed with weight units in the container type for wall-planting during the dry summer season. The soil moisture contents were significantly enhanced in the container type for wall-planting in increasing order as the amount of soil conditioner level was increased ($A_1L_1$ > $A_2L_1$ > $A_4L_1$ > $A_8L_1$ > $A_1L_0$). Compared to the control treatment application (amended soil with 100% + organic soil conditioner 0%), the highest plant growth was observed in the treatment of $A_1L_1$ application (amended soil with 50% + organic soil conditioner 50%). However, the differences between the organic soil conditioner ratio treatments of $A_1L_1$, $A_4L_1$, and $A_8L_1$ organic soil conditioner application were mostly not significant. The survival rate increased with the increasing application of organic soil conditioner, but in the control treatment application all the plants died. The experimental results from clearly demonstrated that the organic soil conditioner improved the survival rate more than the growth of Cotoneaster horizontalis. Therefore, Cotoneaster horizontalis is expected to be a highly valuable shrub for green wall systems, when considered for us in integration system or for increasing soil water contents in planting grounds.

Development of Vegetation Indicator for Assessment of Naturalness in Stream Environment (하천환경의 자연성 평가를 위한 식생지표의 개발)

  • Chun, Seung-Hoon;Chae, Soo-Kwon
    • Journal of Environmental Impact Assessment
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    • v.25 no.6
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    • pp.384-401
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    • 2016
  • The vegetation assessment indicator has been developed recently as a biological part of the integrated assessment system for river environment to improve the efficiency of river restoration projects. This study carried out to test the vegetation assessment indicator and to reset its grade criteria on experimental streams. We classified and mapped vegetation communities at the level of physiognomic-floristic composition by each assessment unit. A total of 204 sampling quadrats were set up on the 68 assessment units at 5 experimental streams. By analyzing the vegetation data collected, we examined the appropriate numbers of sampling quadrats, the criteria of vegetation index score, classification of vegetation community, and grade criteria for vegetation assessment. The developed vegetation assessment indicator composed with the vegetation complexity index (VCI), the vegetation diversity index (VDI), and the vegetation naturalness index (VNI) was proved to reflect the current conditions of the streams sufficiently. The contribution of vegetation naturalness index to grading by vegetation assessment indicator was larger, but three indexes were closely correlated to each other. Also there was more clearer discrimination of grading with the application of adjusted criteria of vegetation assessment indicator and the standardized classification of vegetation community, but the stream segment type did not influence the vegetation assessment grade significantly.

Effects of Feeding Earthworm Meal on the Egg Quality and Performance of Laying Hens (지렁이 분말의 급여가 계란의 품질 및 산란계의 생산성에 미치는 영향)

  • Son J.H.
    • Korean Journal of Poultry Science
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    • v.33 no.1
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    • pp.41-47
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    • 2006
  • A study was conducted to investigate the effect of supplementing earthworm meal(EWM) on the egg quality and performance of laying hens. A total of 360 laying hens at 55 weeks of age were fed the experimental diets containing 0(Control), 0.3 and 0.6% of EWM for 5 weeks. Eggs were collected and weighed in every day and egg production and feed conversion were weekly recorded. However egg quality were measured fer last week of experimental period. When fed both 0.3 and 0.6% of EWM, egg production and daily egg mass tended to increase but were not different between those treatments. Feed intake and feed conversion ratio of laying hens were not different among three groups. Egg shell thickness, breaking strength, color and egg yolk color were tend to improve in both 0.3 and 0.6% of EWM compared to those of control. The haugh units(HUs) showed no difference among each treatments at 14 after laying egg, but increased in EWM treatments compared to control for storage period. As, Cd, Cr, Hg and Pb detected 4.41, 1.23, 1.18, 0.00 and 3.39ppm in EWM, respectively, but which were not detected in control. It assumed that supplementing 0.3% of earthworm meal in the 55 weeks old laying hens diet, improved the laying performance and egg quality.

The growth and productivity of native Indonesian rice progenies and its relationship with root development during dry-season

  • Zakaria, Sabaruddin;Fitrya, Farid;Kurniawan, Trisda;Hereri, Agam Ihsan;Maulana, Teuku
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.338-338
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    • 2017
  • One of the problems in increasing rice production is getting lines or varieties that have high adaptability so that able to produce maximum production in a variety of environmental conditions. One strategy that can be done to get adaptive varieties is through the improvement of native varieties. This research was conducted in rain fed paddy field, Aceh province, Indonesia from June to September 2014. The texture of the soil was clay-loam with the soil pH ranged from 6.5-6.7. Five potential progenies of rice from crossing between native Indonesian rice with introduced rice varieties consist of C3, C4, S3, S5, S6 were used in this study. Besides that, one national rice variety Ciherang also used as a comparison. The plants were growth in the plot with the size of $2m{\times}1.4m$ with plant distance was $20cm{\times}20cm$. The fertilizers used in this study were Urea, NPK, and KCl. Randomized block design with 6 rice progenies/variety and 3 replications were used in this study. There were 18 experimental units and each experimental unit had 10 samples for the sources of data. The variables that were observed in this study including plant height at harvesting time, number of productive tiller, the percentage of empty grains and filled grain per panicle, weight of filled grains per hill, weight of filled grain per plot and yield potential per hectare. Analyzed were also conducted for the depth of root penetration, dry-root weight, dry-shoot weight, shoot-root weight ratio and its correlation with the weight of filled grain per hill. The research results show that there was significant difference on plant height at harvesting time, number of productive tillers, the percentage of empty grains and filled grain per panicle, weight of filled grains per hill, weight of filled grain per plot and yield potential per ha (p>0.01) among the treatments. In addition, depth root penetration, dry-root weight, dry-shoot weight, shoot-root weight ratio also had significant difference (p>0.01) among the treatment. The highest plant at harvesting time was found in S6, reaching 129.8 cm and the shortest plants was found in C3 reaching 107.5 cm. The largest number of productive tillers and the highest percentage of filled grains per panicle were found in Ciherang reaching 10.5 tillers and 80.7% respectively. Ciherang also had the heaviest weight of filled grains per hill and per plot reaching 21.1 g and 2.18 kg respectively. Whereas, S6 had the lowest number of tillers and the lowest percentage of filled grain per panicle.. The highest yield potential per ha was found in Ciherang reaching 7.79 tons. Among the progenies, S5 had the highest yield potential reaching 5 tons/Ha. The result also showed significant relationship between shoot-root weight ratio with weight of filled grains per hill. The highest value of shoot-root weight ratio (1.57) in Ciherang is thought had closed relationship with its yield potential.

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Modification of Microclimate to Improve Milk Production in Tropical Rainforest of Thailand

  • Suriyasathaporn, W.;Boonyayatra, S.;Kreausukon, K.;Pinyopummintr, T.;Heuer, C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.6
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    • pp.811-815
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    • 2006
  • The objective of this study was to evaluate the effect of electric fan installation for milk production improvement of dairy cattle in Thailand. The study was conducted using 2 small-holder dairy farms in Chiang Mai province, during April to August 2004. Electric fans were installed in front of each row of cows. Each of the two rows of cows in the barn was defined as an experimental unit, thus each farm had two experimental units. The fans were operated alternately in 7-day intervals between rows of cows within each farm during the day or between 8.00 am to 8.00 pm. Non-operation periods were used as control. Milk yields were recorded. Data on environmental temperature and humidity were obtained from Chiang Mai Meteorological Center. Result from statistically analysis of milk record suggested an interaction between lactation period and fan installation. Therefore, this interaction term of lactation period and fan installation (PERIOD_FAN) was added as a variable to the regression model. Due to the repeated data collection of milk yield from the same cow (alternate week), milk yield was analyzed by repeated measure analysis (Mixed model). Least square means were calculated for all levels and used to compare between each pair-wise values. The final data were collected from the total of 18 cows with 2,072 data. Overall means and SEM of milk yields and days in milk separated into farm were $14.7{\pm}0.06kg/day$ and $176.3{\pm}2.2days$, and $15.2{\pm}0.22kg/day$ and $202.5{\pm}3.7$ days for farm A and farm B, respectively. For multivariable analysis, only PERIOD_FAN and humidity were significantly associated with milk yield. Only the first period of lactation showed that the amount of milk yields during fan installation was higher than that of non-fan installation (p<0.05). Cows with fan installation produced approximately 1.2 kg/cow more milk than cows without fan installation during this period. In conclusion, the use of electric fan operated during the day time increased milk production of cows during the first period of lactation.

The Effect of Continuous Use of Cow Manure on Productivity of Organic Rice and Feed Value, Stock Carrying Capacity of Organic Rice Straw in Central Region of Korea (한국 중부지역에서 우분의 연용이 유기 벼의 생산성 및 유기 볏짚의 사료가치, 가축사육능력에 미치는 영향)

  • Park, Sang-Soo;Noh, Jin-Hwan;Lee, Hee-Choong;Kim, Yoon-Joong;Lee, Ju-Sam
    • Korean Journal of Organic Agriculture
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    • v.21 no.2
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    • pp.271-282
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    • 2013
  • This experiments was conducted to investigate the influence on organic rice cultivation by continuous use of cow manure in the central region of Korea and investigated productivity of organic rice and feed value, stock carrying capacity of organic rice straw. In the results, application of chemical fertilizer showed higher one of 1st and 2nd year in fresh matter yield (15.77, 30.30ton/ha), dry matter yield (9.90, 13.89 ton/ha), dry matter yield of rice straw (4.88, 7.15ton/ha), dry matter weight of kernel per plant (29.81, 39.99g) and number of kernel per plant (1212.58, 1701units), but there were not significantly differences with each experimental plots in 3rd year. And harvest index showed higher one in application of chemical fertilizer (0.52, 0.48, 0.43) until 3 years. But their quantitative difference between application of chemical fertilizer and cow manure was gradually decreased each year. And the average value of $K_{CP}$ and $K_{TDN}$ showed higher one in application of chemical fertilizer (2.01, 2.94, 1.95 head/ha/yr) until 3 years. However, quantitative difference between chemical fertilizer and cow manure was gradually decreased each year. Especially, application of cow manure showed higher value of 3rd year in $K_{ME}$, but there was not significantly difference with each experimental plots. According to the results, continuous use of cow manure will be reduced their quantitative differences between chemical fertilizer as the year progress in the central region of Korea in terms of productivity of organic rice and feed value, stock carrying capacity of organic rice straw.

Calculation of Stability Number of Tetrapods Using Weights and Biases of ANN Model (인공신경망 모델의 가중치와 편의를 이용한 테트라포드의 안정수 계산 방법)

  • Lee, Jae Sung;Suh, Kyung-Duck
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.28 no.5
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    • pp.277-283
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    • 2016
  • Tetrapod is one of the most widely used concrete armor units for rubble mound breakwaters. The calculation of the stability number of Tetrapods is necessary to determine the optimal weight of Tetrapods. Many empirical formulas have been developed to calculate the stability number of Tetrapods, from the Hudson formula in 1950s to the recent one developed by Suh and Kang. They were developed by using the regression analysis to determine the coefficients of an assumed formula using the experimental data. Recently, software engineering (or machine learning) methods are introduced as a large amount of experimental data becomes available, e.g. artificial neural network (ANN) models for rock armors. However, these methods are seldom used probably because they did not significantly improve the accuracy compared with the empirical formula and/or the engineers are not familiar with them. In this study, we propose an explicit method to calculate the stability number of Tetrapods using the weights and biases of an ANN model. This method can be used by an engineer who has basic knowledge of matrix operation without requiring knowledge of ANN, and it is more accurate than previous empirical formulas.