• Journal of Applied Biological Chemistry
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• v.63 no.1
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• pp.69-74
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• 2020

The fruit, leaves, and roots of Elaeagnus multiflora Thunb. have been used in traditional Chinese medicine to treat cough, diarrhea, and itching. However, the anti-fatigue effects of E. multiflora fruit (EMF) extract have not been studied in detail. The aim of this study was to examine the effect of EMF on fatigue and exercise performance in BALB/c mice. EMF was orally administered to mice at four doses (10, 50, 100, and 200 mg/kg/day) for 2 weeks. The anti-fatigue activity was evaluated by determining the exhaustive swimming time. Blood lactate and glucose levels and serum lactate levels after a 10 min swimming time, as well as ammonia, creatine kinase (CK), blood urea nitrogen (BUN), lactate dehydrogenase (LDH), and glycogen contents after exhaustive swimming time were measured. The exhaustive swimming time of the EMF 200 group was significantly increased (p <0.01). The EMF groups showed significantly low levels of CK, BUN, LDH, and lactate compared with the control group (p <0.05). Increased liver glycogen was observed in the EMF 200 group (p <0.05). These results suggest that EMF can be utilized as an efficacious natural resource for its anti-fatigue effects.

• Journal of Veterinary Clinics
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• v.27 no.3
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• pp.262-267
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• 2010

Magnesium ($Mg^{2+}$) is an essential co-factor for over 325 physiological and biochemical processes so that plays a central role of neuronal activity, cardiac excitability, neuromuscular transmission, muscular contraction, vasomotor tone, and blood pressure significantly related to physical performance. However, only limited information on blood ionized $Mg^{2+}$ ($iMg^{2+}$) regarding to physical exercise is available and the data from blood total $Mg^{2+}$ detection are inconsistent. This present study investigated the changes of blood $iMg^{2+}$ correlated with metabolic demands during acute high-intensive exhaustive physical exercise in rats. After exhausted swimming (3-4 hours), blood pH, glucose, $HCO_3{^-}$, oxygen and ionized $Ca^{2+}$ ($iCa^{2+}$) were significantly decreased, whereas lactate, carbon dioxide, $iMg^{2+}$, ionized $Na^+$ and ionized $K^+$ were significantly increased. During the exhausted swimming, the changes in $iMg^{2+}$ showed a significant negative correlation with changes in pH, glucose, $HCO_3^-$ and $iCa^{2+}$, however a significant negative correlation with changes in lactate and anionic gap. It is concluded that the acute high-intensive exhaustive physical exercise could produced hypermagnesemia, an increase in blood $iMg^{2+}$ via stimulation of $iMg^{2+}$ efflux following increase in intracellular $iMg^{2+}$ from muscle induced by metabolic and respiratory acidosis.

• Preventive Nutrition and Food Science
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• v.20 no.4
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• pp.246-252
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• 2015

The hot water extract of leather carp (Cyprinus carpio nudus) has been used as a nourishing tonic soup and as an aid for recovery from physical fatigue. In this study, we investigated the effect of leather carp extract on exercise performance in mice. Swimming endurance and forelimb grip strength were assessed following oral administration of the extract (once per day for 7 days) at a dose of $0.5 mg/10{\mu}L/g$ body weight. After 7 days, mice given the leather carp extract had significantly greater swimming endurance [$105{\pm}18s$ (P<0.05); 52% longer than day 0] and forelimb grip strength [$1.18{\pm}0.05$ Newton (P<0.01); 17% greater than day 0]. The extract increased muscle mass, but had little effect on body weight. Following the swimming exercise, blood glucose, glutathione peroxidase, and superoxide dismutase levels in extract-fed mice were significantly higher (145%, 131%, and 106%, respectively) than in the saline control group. Blood levels of high-density lipoprotein cholesterol were also significantly increased (128%) in mice given the extract compared to the controls. These results suggest that leather carp extract can improve physical exercise performance and prevent oxidative stress caused by exhaustive workouts.

• Journal of Korean Medicine Rehabilitation
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• v.30 no.2
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• pp.37-46
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• 2020

Objectives In this study, we analyzed the effect of Dioscoreae Rhizoma on exercise practice ability and blood fatigue variable factor in the forced swimming test. Methods Male mice were divided into four group. Mice were administrated with saline, Discorae Rhizoma extracted by water (DRW), Discorae Rhizoma extracted by 30% ethanol (DRE), red ginseng (RG), orally once per day, for 1 week. Exercise performance was evaluated by exhaustive swimming time and weights. Glycogen contents of liver and soleus muscle were measured at the end of the experiment. In addition, the biochemical parameters such as asparate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen, creatine, lactate dehydrogenase (LDH), ammonia, triglycerides (TG), total cholesterol (TC) and free fatty acid (FFA) were analyzed. Results Both DRW and DRE showed an improvement of swimming endurance compared with control group. Levels of ALT, ammonia, TG, TC and FFA were significantly decreased in both DRW and DRE compared with control group. Levels of AST, Creatine were significantly reduced in DRE group compared with control group. Moreover LDH showed only a tendency to increase in the DRW and DRE groups. Conclusions In summary, the present study showed that Dioscoreae Rhizoma improved in physical fatigue and exercise practice ability and DRE improved the superior than those of DRW.

• Preventive Nutrition and Food Science
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• v.20 no.2
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• pp.133-136
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• 2015

Freshwater softshell turtle (Trionyx sinensis) extract has been used traditionally as a tonic soup, and to recover from physical fatigue. To support these claims, the forelimb grip strength of mice was measured after feeding a soft-shell turtle extract for 7 days. The T. sinensis extract significantly increased the grip strength to $1.25{\pm}0.07N$ (P<0.01), which is 16.8% higher than the force on day 0. After exercising, the blood glucose levels in extract-fed mice were 202% higher and urea levels were 73% lower, which were both significantly different than the levels observed after control treatment. Lactate dehydrogenase was significantly higher by 314%, and glutathione peroxidase increased by 165%. In addition, the obesity markers, serum triglyceride and cholesterol, decreased to 62% and 49%, respectively, after mice were fed the extract. These data show that the T. sinensis extract provided more energy for forelimb exercise, prevented protein catabolism and muscle fatigue, and decreased the oxidative stress caused by an exhaustive workout.

• The Korean Journal of Physiology
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• v.5 no.2
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• pp.15-27
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• 1971

Physiological analysis of the physical exercise was made on 9 subjects performing mountain climbing. The course between two points (256 and 516 meters altitude) was 1,300 meters in distance and difference of vertical height was 260 meters making the mean grade of 20%. In the field, the heart rates during uphill or downhill walk were recorded by EKG radio-telemetry. In the laboratory, oxygen consumption was obtained by the recorded heart rates, using individual heart rate vs oxygen consumption diagram obtained by treadmill test. the following results were obtained. 1. Uphill walk time was 36.5 minutes, and during this period the mean heart rate was 149.0 heats/min and peak heart rate was 169.2 beats/min. The total heart beats during the uphill walk was 5.433 beats. 2. The ratio of individual mean heart rate during the uphill walk to the maximal heart rate distributed between 66.6% and 98.3%, and the mean of the total group was 83.1%. The ratio of peak heart rate of uphill walk to the maximal heart rate was 94.5% in the group. Thus uphill walk of a 20% grade mountain course was an exhaustive exercise. 3. Oxygen consumption during uphill walk was 2.22 l/min (ranged between 1.79 and 2.70 l/min) and the ratio of this to the resting oxygen consumption was 8.31. The peak value of oxygen consumption during uphill walk was 2.73 l/min and the ratio of this to the resting oxygen consumption was 10.39. 4. Energy expenditure during uphill walk showed a mean of 11.1 kcal/min and the peak expenditure rate was 13.6 kcal/min. The total energy expenditure during 36.5 minutes of uphill walk was 396 kcal. 5. In downhill walk, the time was 31.7 minutes, mean heart rate was 118.4 (ranged between 100.1 and 142.7) beats/min, and the peak heart rate was only 129.4 beats/min. The ratio of mean heart rate to the maximal heart rate was 66.3%. Total heart beats during downhill walk was 3,710 beats. The ratio of downhill oxygen consumption to the resting consumption was 5.70. The rate of energy expenditure was 7.5 kcal/min, and the total onery expenditure during the 31.7 minutes of downhill walk was 228 kcal. 6. The effect of training was manifest in the uphill walk and not in the downhill walk. After training in mountain course walk, i) the uphill time was shortened, ii) mean heart rate increased, iii) time vs heart rate curve became smooth and showed less frequent zig-zag, i.e., the depth of trough on the curve decreased and the magnitude was less than 10 beats. In non-trained subject the depth of trough on the curve was greater than 50 beats and appeared more frequently. 7. Mountain climbing is a good health promotion exercise. For the promotion of health the reasonable amount of uphill mountain walk exercise in a 20% grade course is a walk for 40 or 50 minutes duration once a week.

• Korean Journal of Exercise Nutrition
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• v.16 no.1
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• pp.51-59
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• 2012

The purpose of this study was to examine the effects of endurance training and prolonged L-arginine supplementation on blood glucose, blood insulin, muscle glycogen, muscle glycogen synthase (GS), muscle nitric oxide (NO), muscle nitric oxide synthase (NOS), endurance performance. We equally divided 36 Sprague-Dawley mice to be distributed into control group, L-NMMA treated group and L-arginine treated group. The L-arginine treated group and L-NMMA treated group consumed 10 mg/kg/day of L-arginine and 5 mg/kg/day of L-NMMA for 6 weeks period. Mice of control group, L-arginine treated group, and L-NMMA treated groups performed swimming exercise training for 60 min once a day, 5 days per week for 6 weeks. Blood glucose had tendency to increase in L-arginine treated group than the control group, and insulin significantly increased in L-arginine treated group than the control group. L-arginine treated group showed significant increase in glycogen, GS, NO and NOS in the gastrocnemius muscle and soleus muscle compared to the control group. Whereas L-NMMA treated group showed the lowest glycogen, GS, NO and NOS in the gastrocnemius muscle and soleus muscle compared to control group and L-arginine treated group. Exhaustive swimming time had tendency to increase in L-arginine treated group compared to the value for control group. These reults indicate that endurance training and prolonged L-arginine supplementation appear to be effective in exhancing nitric oxide production, glycogen concentration and endurance performance.

• Korean Journal of Environment and Ecology
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• v.9 no.1
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• pp.49-55
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• 1995

To investigate the heart rate changes under the influence of physical conditions on mountain trails, heart rates of 3 subjects were recorded on 9 routes in Kyeryongsan National Park and Odaesan National Park. Maximal heart rate during uphill walking recorded between 84% and 96% to individual maximal heart rate using ergometer, thus uphill walking on mountain trail was an exhaustive exercise. The ratio of maximal heart rate during downhill walking indicated between 78% and 93% to maximal heart rate during uphill walking. And gradient of trail influenced on heart rate, but width of trail didn't. The heart rate on wooden- or stony-step-trail were rapidly increased in comparison with the heart rates on soil-trails.

• Journal of Life Science
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• v.20 no.1
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• pp.147-152
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• 2010

Exercise-induced anaphylaxis (EIA) is a physical allergy, sometimes severe, triggered by exertion following specific food intake. It was defined for the first time in 1980. EIA is associated with different kinds of exercise. The clinical manifestations progress from itching, erythema and urticaria to some combination of cutaneous angioedema and vascular collapse. Mast cell participation in the pathogenesis of this syndrome has been proved by the findings of an elevated serum histamine level during exhaustive exercise. As predisposing factors of EIA, a specific or even nonspecific sensitivity to food has been reported. Food-dependent exercise-induced anaphylaxis (FDEIA) is a distinct form of food allergy induced by physical exercise. It is typified by the onset of anaphylaxis during exercise which was preceded by the ingestion of the causal food allergens. The diagnosis of FDEIA is heavily dependent on clinical history. Allergy tests may need to be performed using a broad panel of food and food additives. As with food allergies, FDEIA diagnosis is based on interview, biological test and skin test. Prophylaxis aims to prevent a recurrence; the patient should be given an emergency kit to deal with any recurrent episodes. After the food allergen has been identified, it should be avoided for at least 4 to 5 hours before any exercise. Two cases of EIA are presented (EIA to circumstances; FDEIA) in this paper, The diagnosis, pathophysiology and therapy of FDEIA are also reviewed.

• Journal of the Economic Geographical Society of Korea
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• v.13 no.3
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• pp.416-441
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• 2010

This paper attempts to develop a framework articulating a suite of indicators of cluster development, based upon existing work on the economic geography of clusters, cluster frameworks and indicators and cluster policies. Unlike other work the framework adopted here emphasizes adaptive and proactive roles played by agents, whether individual or collective, within the cluster when understanding it as a learning environment to capture an implication made from adopting the cluster perspective. Some possible indicators are operationalized and suggested even if they are not definitive and exhaustive. The conceptual framework and the specific indicators suggested can provide policy-makers and key stakeholders in clusters with a proper set of tools for measuring the level of cluster development, maneuvering a broader strategic planning exercise for successful cluster development.