Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Oral Administration of a Hot Water Extract of the Softshell Turtle (Trionyx sinensis) Improves Exercise Performance

  • Harwanto, Dicky (Department of Biotechnology, Pukyong National University) ;
  • Lee, Gong-Hyeon (Department of Biotechnology, Pukyong National University) ;
  • Park, Sun-Mee (Institute of Marine Biotechnology, Pukyong National University) ;
  • Choi, Jae-Suk (Department of Bio-Food Materials, Silla University) ;
  • Kim, Mi-Ryung (Department of Bio-Food Materials, Silla University) ;
  • Hong, Yong-Ki (Department of Biotechnology, Pukyong National University)
  • Received : 2015.03.02
  • Accepted : 2015.06.04
  • Published : 2015.06.30
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Abstract

Freshwater softshell turtle (Trionyx sinensis) extract has been used traditionally as a tonic soup, and to recover from physical fatigue. To support these claims, the forelimb grip strength of mice was measured after feeding a soft-shell turtle extract for 7 days. The T. sinensis extract significantly increased the grip strength to $1.25{\pm}0.07N$ (P<0.01), which is 16.8% higher than the force on day 0. After exercising, the blood glucose levels in extract-fed mice were 202% higher and urea levels were 73% lower, which were both significantly different than the levels observed after control treatment. Lactate dehydrogenase was significantly higher by 314%, and glutathione peroxidase increased by 165%. In addition, the obesity markers, serum triglyceride and cholesterol, decreased to 62% and 49%, respectively, after mice were fed the extract. These data show that the T. sinensis extract provided more energy for forelimb exercise, prevented protein catabolism and muscle fatigue, and decreased the oxidative stress caused by an exhaustive workout.

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References

  1. Mehta RK, Agnew MJ. 2012. Influence of mental workload on muscle endurance, fatigue, and recovery during intermittent static work. Eur J Appl Physiol 112: 2891-2902. https://doi.org/10.1007/s00421-011-2264-x
  2. Marquez R, Santangelo G, Sastre J, Goldschmidt P, Luyckx J, Pallardo FV, Vina J. 2001. Cyanoside chloride and chromocarbe diethylamine are more effective than vitamin C against exercise-induced oxidative stress. Pharmacol Toxicol 89: 255-258. https://doi.org/10.1034/j.1600-0773.2001.d01-156.x
  3. Wang J, Li S, Fan Y, Chen Y, Liu D, Cheng H, Gao X, Zhou Y. 2010. Anti-fatigue activity of the water-soluble polysaccharides isolated from Panax ginseng C. A. Meyer. J Ethnopharmacol 130: 421-423. https://doi.org/10.1016/j.jep.2010.05.027
  4. Huang CC, Hsu MC, Huang WC, Yang HR, Hou CC. 2012. Triterpenoid-rich extract from Antrodia camphorata improves physical fatigue and exercise performance in mice. Evid Based Complement Alternat Med 2012: 364741.
  5. Ni W, Gao T, Wang H, Du Y, Li J, Li C, Wei L, Bi H. 2013. Anti-fatigue activity of polysaccharides from the fruits of four Tibetan plateau indigenous medicinal plants. J Ethnopharmacol 150: 529-535. https://doi.org/10.1016/j.jep.2013.08.055
  6. Chen TH, Chang HC, Lue KY. 2009. Unregulated trade in turtle shells for Chinese traditional medicine in East and Southeast Asia: the case of Taiwan. Chelonian Conserv Biol 8: 11-18. https://doi.org/10.2744/CCB-0747.1
  7. Park JY. 2013. Korean Fisheries Yearbook. 41st ed. Choi GR, ed. Korea Fisheries Association, Seoul, Korea. p 507.
  8. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-275.
  9. Li X, Mohan S, Gu W, Wergedal J, Baylink DJ. 2001. Quantitative assessment of forearm muscle size, forelimb grip strength, forearm bone mineral density, and forearm bone size in determining humerus breaking strength in 10 inbred strains of mice. Calcif Tissue Int 68: 365-369. https://doi.org/10.1007/s00223-001-0004-7
  10. Golde WT, Gollobin P, Rodriguez LL. 2005. A rapid, simple, and humane method for submandibular bleeding of mice using a lancet. Lab Anim (NY) 34: 39-43.
  11. Saggu S, Kumar R. 2008. Effect of seabuckthorn leaf extracts on circulating energy fuels, lipid peroxidation and antioxidant parameters in rats during exposure to cold, hypoxia and restraint (C-H-R) stress and post stress recovery. Phytomedicine 15: 437-446. https://doi.org/10.1016/j.phymed.2007.11.002
  12. Liu J, Willför S, Xu C. 2015. A review of bioactive plant polysaccharides: biological activities, functionalization, and biomedical applications. Bioact Carbohydr Diet Fibre 5: 31-61. https://doi.org/10.1016/j.bcdf.2014.12.001
  13. Garrett RH, Grisham CM. 2006. Biochemistry. 3rd ed. Brooks/Cole Publishing Co., Belmont, CA, USA. p 1086.
  14. Kumar GP, Anand T, Singsit D, Khanum F, Anilakumar KR. 2013. Evaluation of antioxidant and anti-fatigue properties of Trigonella foenum-graecum L. in rats subjected to weight loaded forced swim test. Pharmacogn J 5: 66-71. https://doi.org/10.1016/j.phcgj.2013.03.005
  15. Washington TA, Healey JM, Thompson RW, Lowe LL, Carson JA. 2014. Lactate dehydrogenase regulation in aged skeletal muscle: regulation by anabolic steroids and functional overload. Exp Gerontol 57: 66-74. https://doi.org/10.1016/j.exger.2014.05.003
  16. Bigard AX, Zoll J, Ribera F, Mateo P, Sanchez H, Serrurier B, Ventura-Clapier R. 2001. Influence of overload on phenotypic remodeling in regenerated skeletal muscle. Am J Physiol Cell Physiol 281: C1686-C1694. https://doi.org/10.1152/ajpcell.2001.281.5.C1686
  17. Qi B, Liu L, Zhang H, Zhou GX, Wang S, Duan XZ, Bai XY, Wang SM, Zhao DQ. 2014. Anti-fatigue effects of proteins isolated from Panax quinquefolium. J Ethnopharmacol 153: 430-434. https://doi.org/10.1016/j.jep.2014.02.045
  18. Wu CY, Chen R, Wang XS, Shen B, Yue W, Wu Q. 2013. Antioxidant and anti-fatigue activities of phenolic extract from the seed coat of Euryale ferox Salisb. and identification of three phenolic compounds by LC-ESI-MS/MS. Molecules 18: 11003-11021. https://doi.org/10.3390/molecules180911003
  19. Cai Q, Rahn RO, Zhang R. 1997. Dietary flavonoids, quercetin, luteolin and genistein, reduce oxidative DNA damage and lipid peroxidation and quench free radicals. Cancer Lett 119: 99-107. https://doi.org/10.1016/S0304-3835(97)00261-9
  20. Walberg JL, Greenwood MR, Stern JS. 1983. Lipoprotein lipase activity and lipolysis after swim training in obese Zucker rats. Am J Physiol 245: 706-712.
  21. Jung KA, Kim IH, Han DS. 2004. Effect of medicinal plant extracts on forced swimming capacity in mice. J Ethnopharmacol 93: 75-81. https://doi.org/10.1016/j.jep.2004.03.022

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