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Fabrication of Fluorescent Labeled Bi-compartmental Particles via the Micromolding Method (미세 성형 방법을 이용한 형광 표지된 이중 분획 입자의 제조)

  • Shim, Gyurak;Jeong, Seong-Geun;Hong, Woogyeong;Kang, Koung-Ku;Lee, Chang-Soo
    • Korean Chemical Engineering Research
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    • v.56 no.6
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    • pp.826-831
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    • 2018
  • This study presents fabrication of bi-compartmental particles labeled by multiple fluorescence. To compartmentalize fluorescent expression at the particle, two fluorescent dyes with less overlap of the excitation and emission spectra are selected. To ensure the fluorescence stability, the fluorescent dyes contain acrylate functional groups in the molecules so that they can be cross-linked together with monomers constituting the particle. Strong fluorescent expression and compartmentalization were observed at the particle fabricated using the selected fluorescent dyes through confocal microscopy. Furthermore, long-term fluorescence stability was verified by measuring fluorescent expression and intensity for 4 weeks. We anticipate that the bi-compartmental particles labeled by multiple fluorescence can be widely used for multi-target drug delivery system, analysis of 3 dimensional Brownian motion, and investigation of 3 dimensional complex self-assembled morphologies.

Synthesis and Optical Properties of BaSiO3:RE3+ (RE = Sm, Eu) Phosphors (BaSiO3:RE3+ (RE = Sm, Eu) 형광체의 합성과 광학 특성)

  • Cho, Shinho
    • Korean Journal of Materials Research
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    • v.29 no.6
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    • pp.356-362
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    • 2019
  • $BaSiO_3:RE^{3+}$ (RE = Sm or Eu) phosphor powders with different concentrations of activator ions are synthesized using the solid-state reaction method. The effects of the concentration of activator ions on the structural, photoluminescent, and morphological properties of the barium silicate phosphors are investigated. X-ray diffraction data reveals that the crystal structure of all the phosphors, regardless of the type and the concentration of the activator ions, is an orthorhombic system with a main (111) diffraction peak. The grain particles agglomerate together to form larger clusters with increasing concentrations of activator ions. The emission spectra of the $Sm^{3+}$-doped $BaSiO_3$ phosphors under excitation at 406 nm consist of an intense orange band at 604 nm and three weak bands centered at 567, 651, and 711 nm, respectively. As the concentration of $Sm^{3+}$ increases from 1 to 5 mol%, the intensities of all the emission bands gradually increase, reach maxima at 5 mol% of $Sm^{3+}$ ions, and then decrease significantly with further increases in the $Sm^{3+}$ concentration due to the concentration quenching phenomenon. For the $Eu^{3+}$-doped $BaSiO_3$ phosphors, a strong red emission band at 621 nm and several weak bands are observed. The optimal orange and red light emissions of the $BaSiO_3$ phosphors are obtained when the concentrations of $Sm^{3+}$ and $Eu^{3+}$ ions are 5 mol% and 15 mol%, respectively.

Seismic responses of a free-standing two-story steel moment frame equipped with a cast iron-mortar sliding base

  • Chung, Yu-Lin;Kuo, Kuan-Ting;Nagae, Takuya;Kajiwara, Koichi
    • Earthquakes and Structures
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    • v.17 no.3
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    • pp.245-256
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    • 2019
  • An experimental study was conducted to evaluate the dynamic behavior of a free-standing frame equipped with a movable base system using cast iron and mortar as the bearing materials. The preliminary friction test indicated that a graphite layer developed on the interface and exhibited stable friction behavior. The friction coefficient ranged from 0.33 to 0.36 when the applied normal compression stress ranged from 2.6 to 5.2 MPa. The effect of the variation of normal compression stress would be small. Shaking table tests on the free-standing frame showed that rock, slide, and rock-slide responses occurred. The cumulative slide distance reached 381 mm under JMA Kobe wave excitation; however, only a few cyclic slides occurred at the same locations along the moving track. Most surfaces sustained single slides. Similar results can be observed in other shaking conditions. The insufficient cyclic sliding and significant rocking resulted in a few graphite layers on the mortar surfaces. Friction coefficients were generally similar to those obtained in the preliminary friction tests; however, the values fluctuated when the rocking became significant. The collisions due to rocking caused strong horizontal acceleration responses and resulted in high friction coefficient. In addition, the strong horizontal acceleration responses caused by the collisions made the freestanding specimen unable to reduce the input horizontal acceleration notably, even when slippage occurred. Compared with the counterpart fixed-base specimen, the specimen equipped with the iron-mortar base could reduce the horizontal acceleration amplification response and the structural deformation, whereas the vertical acceleration response was doubled due to collisions from rocking.

b0 Dependent Neuronal Activation in the Diffusion-Based Functional MRI

  • Kim, Hyug-Gi;Jahng, Geon-Ho
    • Progress in Medical Physics
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    • v.30 no.1
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    • pp.22-31
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    • 2019
  • Purpose: To develop a new diffusion-based functional MRI (fMRI) sequence to generate apparent diffusion coefficient (ADC) maps in single excitation and evaluate the contribution of b0 signal on neuronal changes. Materials and Methods: A diffusion-based fMRI sequence was designed with single measurement that can acquire images of three directions at a time, obtaining $b=0s/mm^2$ during the first baseline condition (b0_b), followed by 107 diffusion-weighted imaging (DWI) with $b=600s/mm^2$ during the baseline and visual stimulation conditions, and another $b=0s/mm^2$ during the last activation condition (b0_a). ADC was mapped in three different ways: 1) using b0_b (ADC_b) for all time points, 2) using b0_a (ADC_a) for all time points, and 3) using b0_b and b0_a (ADC_ba) for baseline and stimulation scans, respectively. The fMRI studies were conducted on the brains of 16 young healthy volunteers using visual stimulations in a 3T MRI system. In addition, the blood oxygen level dependent (BOLD) fMRI was also acquired to compare it with diffusion-based fMRI. A sample t-test was used to investigate the voxel-wise average between the subjects. Results: The BOLD data consisted of only activated voxels. However, ADC_ba data was observed in both deactivated and activated voxels. There were no statistically significant activated or deactivated voxels for DWI, ADC_b, and ADC_a. Conclusions: With the new sequence, neuronal activations can be mapped with visual stimulation as compared to the baseline condition in several areas in the brain. We showed that ADC should be mapped using both DWI and b0 images acquired with the same conditions.

Analytical and experimental investigation of stepped piezoelectric energy harvester

  • Deepesh, Upadrashta;Li, Xiangyang;Yang, Yaowen
    • Smart Structures and Systems
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    • v.26 no.6
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    • pp.681-692
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    • 2020
  • Conventional Piezoelectric Energy Harvesters (CPEH) have been extensively studied for maximizing their electrical output through material selection, geometric and structural optimization, and adoption of efficient interface circuits. In this paper, the performance of Stepped Piezoelectric Energy Harvester (SPEH) under harmonic base excitation is studied analytically, numerically and experimentally. The motivation is to compare the energy harvesting performance of CPEH and SPEHs with the same characteristics (resonant frequency). The results of this study challenge the notion of achieving higher voltage and power output through incorporation of geometric discontinuities such as step sections in the harvester beams. A CPEH consists of substrate material with a patch of piezoelectric material bonded over it and a tip mass at the free end to tune the resonant frequency. A SPEH is designed by introducing a step section near the root of substrate beam to induce higher dynamic strain for maximizing the electrical output. The incorporation of step section reduces the stiffness and consequently, a lower tip mass is used with SPEH to match the resonant frequency to that of CPEH. Moreover, the electromechanical coupling coefficient, forcing function and damping are significantly influenced because of the inclusion of step section, which consequently affects harvester's output. Three different configurations of SPEHs characterized by the same resonant frequency as that of CPEH are designed and analyzed using linear electromechanical model and their performances are compared. The variation of strain on the harvester beams is obtained using finite element analysis. The prototypes of CPEH and SPEHs are fabricated and experimentally tested. It is shown that the power output from SPEHs is lower than the CPEH. When the prototypes with resonant frequencies in the range of 56-56.5 Hz are tested at 1 m/s2, three SPEHs generate power output of 482 μW, 424 μW and 228 μW when compared with 674 μW from CPEH. It is concluded that the advantage of increasing dynamic strain using step section is negated by increase in damping and decrease in forcing function. However, SPEHs show slightly better performance in terms of specific power and thus making them suitable for practical scenarios where the ratio of power to system mass is critical.

Seismic pounding between adjacent buildings considering soil-structure interaction

  • Raheem, Shehata E Abdel;Alazrak, Tarek M.A.;AbdelShafy, Aly G.A.;Ahmed, Mohamed M.;Gamal, Yasser A.S.
    • Earthquakes and Structures
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    • v.20 no.1
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    • pp.55-70
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    • 2021
  • In urban cities, buildings were built in the neighborhood, these buildings influence each other through structure-soilstructure interaction (SSSI) and seismic pounding due to limited separation distance in-between. Generally, the effects of the interaction between soil and structure are disregarded during seismic design and analysis of superstructure. However, the system of soil-base adversely changes structural behavior and response demands. Thus, the vibration characteristics plus the seismic response of a building are not able to be independent of those in adjacent buildings. The interaction between structure, soil, and structure investigates the action of the attendance of adjacent buildings to the others by the interaction effect of the sub-soil under dynamic disturbances. The main purpose of this research is to analyze the effects of SSSI and seismic pounding on the behavior of adjacent buildings. The response of a single structure or two adjacent structures with shallow raft base lying on soft soil are studied. Three dimensions finite element models are developed to investigate the effects of pounding; gap distance; conditions of soil; stories number; a mass of adjacent building and ground excitation frequency on the seismic responses and vibration characteristics of the structures. The variation in the story displacement, story shear, and story moment responses demands are studied to evaluate the presence effect of the adjacent buildings. Numerical results acquired using conditions of soil models are compared with the condition of fixed support and adjacent building models to a single building model. The peak responses of story displacement, story moment, and story shear are studied.

Channel Attention Module in Convolutional Neural Network and Its Application to SAR Target Recognition Under Limited Angular Diversity Condition (합성곱 신경망의 Channel Attention 모듈 및 제한적인 각도 다양성 조건에서의 SAR 표적영상 식별로의 적용)

  • Park, Ji-Hoon;Seo, Seung-Mo;Yoo, Ji Hee
    • Journal of the Korea Institute of Military Science and Technology
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    • v.24 no.2
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    • pp.175-186
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    • 2021
  • In the field of automatic target recognition(ATR) with synthetic aperture radar(SAR) imagery, it is usually impractical to obtain SAR target images covering a full range of aspect views. When the database consists of SAR target images with limited angular diversity, it can lead to performance degradation of the SAR-ATR system. To address this problem, this paper proposes a deep learning-based method where channel attention modules(CAMs) are inserted to a convolutional neural network(CNN). Motivated by the idea of the squeeze-and-excitation(SE) network, the CAM is considered to help improve recognition performance by selectively emphasizing discriminative features and suppressing ones with less information. After testing various CAM types included in the ResNet18-type base network, the SE CAM and its modified forms are applied to SAR target recognition using MSTAR dataset with different reduction ratios in order to validate recognition performance improvement under the limited angular diversity condition.

A New Approach for Detection of Gear Defects using a Discrete Wavelet Transform and Fast Empirical Mode Decomposition

  • TAYACHI, Hana;GABZILI, Hanen;LACHIRI, Zied
    • International Journal of Computer Science & Network Security
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    • v.22 no.2
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    • pp.123-130
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    • 2022
  • During the past decades, detection of gear defects remains as a major problem, especially when the gears are subject to non-stationary phenomena. The idea of this paper is to mixture a multilevel wavelet transform with a fast EMD decomposition in order to early detect gear defects. The sensitivity of a kurtosis is used as an indicator of gears defect burn. When the gear is damaged, the appearance of a crack on the gear tooth disrupts the signal. This is due to the presence of periodic pulses. Nevertheless, the existence of background noise induced by the random excitation can have an impact on the values of these temporal indicators. The denoising of these signals by multilevel wavelet transform improves the sensitivity of these indicators and increases the reliability of the investigation. Finally, a defect diagnosis result can be obtained after the fast transformation of the EMD. The proposed approach consists in applying a multi-resolution wavelet analysis with variable decomposition levels related to the severity of gear faults, then a fast EMD is used to early detect faults. The proposed mixed methods are evaluated on vibratory signals from the test bench, CETIM. The obtained results have shown the occurrence of a teeth defect on gear on the 5th and 8th day. This result agrees with the report of the appraisal made on this gear system.

Development of a predictive functional control approach for steel building structure under earthquake excitations

  • Mohsen Azizpour;Reza Raoufi;Ehsan Kazeminezhad
    • Earthquakes and Structures
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    • v.25 no.3
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    • pp.187-198
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    • 2023
  • Model Predictive Control (MPC) is an advanced control approach that uses the current states of the system model to predict its future behavior. In this article, according to the seismic dynamics of structural systems, the Predictive Functional Control (PFC) method is used to solve the control problem. Although conventional PFC is an efficient control method, its performance may be impaired due to problems such as uncertainty in the structure of state sensors and process equations, as well as actuator saturation. Therefore, it requires the utilization of appropriate estimation algorithms in order to accurately evaluate responses and implement actuator saturation. Accordingly, an extended PFC is presented based on the H-ifinity (H∞) filter (HPFC) while considering simultaneously the saturation actuator. Accordingly, an extended PFC is presented based on the H-ifinity (H∞) filter (HPFC) while considering the saturation actuator. Thus, the structural responses are formulated by two estimation models using the H∞ filter. First, the H∞ filter estimates responses using a performance bound (𝜃). Second, the H∞ filter is converted into a Kalman filter in a special case by considering the 𝜃 equal to zero. Therefore, the scheme based on the Kalman filter (KPFC) is considered a comparative model. The proposed method is evaluated through numerical studies on a building equipped with an Active Tuned Mass Damper (ATMD) under near and far-field earthquakes. Finally, HPFC is compared with classical (CPFC) and comparative (KPFC) schemes. The results show that HPFC has an acceptable efficiency in boosting the accuracy of CPFC and KPFC approaches under earthquakes, as well as maintaining a descending trend in structural responses.

Single Step Response Based Method for the Simple Identification of Wiener-type Nonlinear Process (단일 계단 응답에 근거한 Wiener형 비선형 공정의 간편한 모델 확인 방법)

  • Sanghun Lim;Jea Pil Heo;Su Whan Sung;Jietae Lee;Friedrich Y. Lee
    • Korean Chemical Engineering Research
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    • v.61 no.1
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    • pp.89-96
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    • 2023
  • The Wiener-type nonlinear model where a static nonlinear block follows a dynamic linear block is widely used to describe the dynamics of chemical processes. A long process excitation step is typically needed to identify this Wiener-type nonlinear model with two blocks. In order to cope with this disadvantage, an identification method for the Wiener-type nonlinear model that uses only a single-step response is proposed here. The proposed method estimates the response of the dynamic linear sub-block from the initial part of the step response, and then the static nonlinear sub-block is identified. Because the only single-step response is used to identify the Wiener-type nonlinear model, there is great benefit in time and cost for obtaining process response. The performance of the proposed identification method with the single-step response is verified through a representative Wiener-type nonlinear process, a pH titration process, and a liquid level system.