• KSBB Journal
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• v.11 no.3
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• pp.288-294
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• 1996

We have investigated the characteristics of recombinant CHO cell growth and erythropoletin(EPO) production at different concentrations of serum and inoculation density. Cell growth and EPO production were increased with the increase of serum concentration and inoculation density. Enhancement of CHO cell growth and EPO production by medium exchange using serum-free medium at the growth phase of cells was studied. It was found that the exchange of culture medium with serum-free medium was favorable for growth of cells and production of EPO. The maximum number of cell and concentration of EPO obtained by exchanging culture medium were $6.2{\times}105cells/$\textrm{cm}^2$ and 7,470units/m1, respectively, compared to $2.1{\times}105cells/\textrm{cm}^2$ and 2,380units/m1 in serum-containing medium without medium exchange. It was observed that CHO cell growth was correlated with EPO production in serum-free media.

• Journal of the Korean Applied Science and Technology
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• v.23 no.1
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• pp.45-53
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• 2006

In this study, we prepared porous cation exchange membrane using polystyrene such as, EPS (expanded polystyrene), SAN (styrene acrylonitrile copolymer) and HIPS (high impactive polystyrene). These polystyrenes were sulfonated by acetyl sulfate to make porous cation exchange membrane such as, SEPS, SSAN, SHIPS. SEM was employed to confirm porous structure of membrane, and IR spectroscopy was used to confirm sulfonation rate of ion exchange membrane. Water and methanol content were also increased with amount of sulfuric acid in reactants. SSAN-20 showed the highest value in water and methanol content. Fixed ion concentration and conductivity was also increased with an amount of sulfuric acid in reactants. Methanol permeability for SEPS-20, SSAN-20, SHIPS-20 was found to be $1.326\;{\times}\;10^{-5}\;cm^2/s$, $1.527\;{\times}\;10^{-5}\;cm^2/s$ and $1.096\;{\times}\;10^{-5}\;cm^2/s$ respectively. From the result of electrodialysis experiment in 0.03 M $Pb(NO_3)_2$ aqueous solution, anion exclusion and cation selection effects were confirmed.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.2
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• pp.204-207
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• 2007

This study was conducted to suggest proper water management practices after and before broadcasting of rice seed on flooded paddy surface at reclaimed saline soil with two different saline levels in Gyehwado Substation of Honam Agricultural Research Institute (HARI) NICS, RDA for two years from 2004 to 2005. The stable seedling stand in low saline soil of 0.1% salinity was obtained by one time of water exchange after soil rotary Whereas in medium saline soilof 0.3% salinity, three times of water exchange was required for the stable seedling stand. Milled rice yield was not affected by frequency of water exchange in low saline soil, while it decreased sharply in one and two times of water exchange compared with three times of water exchange in medium saline soil. Irrigation water immediately after direct seeding increased the number of seeding stand in low saline soil. With the increase in the interval of water exchange after direct seeding, the milled rice yield decreased. Although the continuous water flowing showed the most number of seedling stand and was increased milled rice yield compared with the others interval of water exchange in medium saline soil, the number of seedling stand and milled rice wasn't significantly different up to exchange of two days interval compared with the continuous water flowing.

• Investigative Magnetic Resonance Imaging
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• v.2 no.1
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• pp.73-82
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• 1998

Purpose : A precise NMR technique for measuring the rate of water exchange and cell membrane permeability across the hepatocyte membrane using liver-specific MR contrast agent is described. Materials and Methods : The rat hepatocytes isolated by perfusion of the livers were used for the NMR measurements. All experiments were performed on an IBM field cycling relaxometer operating from 0.02MHz to 60 MHz proton Larmor frequency. spin-echo pulse sequence was empolyed to measure spin-lattice relaxation time, T1. The continuous distribution analysis of water proton T1 data from rat hepatocytes containing low concentrations of the liver specific contrast agent, Gd-EOB-DTPA, modeled by a general two compartment exchange model. Results : The mean residence time of water molecule inside the hepatocyte was approximately 250 msec. The lower limit for the permeability of the hepatocyte membrane was $(1.3{\pm}0.1){\;}{\times}{\;}10^{-3}cm/sec$. The CONTIN analysis, which seeks the natural distribution of relaxation times, reveals direct evidence of the effect of diffusive exchange. the diffusive water exchange is not small in the intracellular space in the case of hepatocytes. Conclusions : Gd-EOB-DTPA, when combined with continuous distribution analysis, provides a robust method to study water exchange and membrane permeability in hepatocytes. Water exchange in hepatocyte is much slower thatn that in red blood cells. Therefore, tissue-specific contrast agent may be used as a functional agent to give physiological information such as cell membrane permeability.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.29 no.3
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• pp.142-147
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• 2017

Improvement of the enthalpy exchange element's energy efficiency is of current interest from anenergy-saving viewpoint. In this study, a LiCl-impregnated spacer was devised as a means to improve the performance of the enthalpy exchange element. Two samples were tested : one with and one without impregnated spacers of $338{\times}338{\times}380mm$ sizes with 2.0 mm channel spacing. The results showed that the temperature efficiencies of the two samples were approximately the same. The humidity efficiency, however, was strongly affected by the LiCl impregnation. The impregnated sample yielded a 9% higher humidity efficiency under cooling and the difference increased to 14% under aheating condition. It was anticipated that more moisture would beadsorbed on the LiCl-impregnated spacers, which was delivered to their roots and eventually to the air in neighboring channels. Separate moisture adsorption tests revealed that both the adsorption rate and the amount of the adsorbed moisture are higher for the LiCl-impregnated specimen.

• Journal of the Korean Chemical Society
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• v.14 no.1
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• pp.85-89
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• 1970

The rate of the bromine-exchange reaction between gallium bromide and isopropyl bromide in nitrobenzene was measured at 19$^{\circ},\;25^{\circ}$ and 40$^{\circ}C$., using isopropyl bromide labelled with Br-82. The results indicated that the exchange reaction was second order with respect to gallium bromide and first order with respect to isopropyl bromide. The third-order rate constant determined at 19$^{\circ}C$. was 3.2 ${\times}10^{-2}l^2{\cdot}mole^{-2}sec^{-1}$. The activation energy, the enthalpy of activation and the entropy of activation for the exchange reaction were also determined.

• Asia-Pacific Journal of Business
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• v.11 no.1
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• pp.61-75
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• 2020

Purpose - The purpose of this study was to examine the efficiency of the foreign exchange markets in Korea, Japan and China. Design/methodology/approach - This study collected 1327 observations each of the daily closing exchange rates of the three currencies against the US dollar for the sample period from January 1, 2015 to January 31, 2020, based on the tests for autocorrelation, unit root tests and GARCH-M(1,1) model estimation. Findings - We have found that the autocorrelation test indicates the lack of autocorrelation and unit root test confirms the existence of unit roots in all times series of the three currencies, respectively. The GARCH-M(1,1) test results, however, suggest that the exchange rates do not follow a random walk process. In conclusion, the recent spot foreign exchange markets in Korea, Japan and China are believed to be informationally inefficient. Research implications or Originality - These findings have practical implications for both individual and institutional investors to be able to obtain excess returns on their investments in the foreign exchange markets in three countries by using appropriate risk management, portfolio strategy, technical analysis, etc. This study provides the first empirical examination on the foreign exchange market efficiency in the three biggest economies in Asia including China, which has been excluded from research due to its exchange rate regime.

• The Korean Journal of Pharmacology
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• v.30 no.1
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• pp.67-73
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• 1994

Na-Ca exchange transports calcium ion either into (reverse mode Na-Ca exchange) or out of the cell (forward mode Na-Ca exchange) according to the direction of driving force produced by the changes in ratio of intra- and extra-cellular Na concentrations. Thus, Na-Ca exchange is regarded as the regulator of myocardial contraction. However, the existence of reverse mode Na-Ca exchange and its role in myocardial contraction is still questioned. Present study was performed to identify the presence of reverse mode Na-Ca exchange and its possible involvement in the regulation of myocardial contraction in rat heart. Using the left atria of rat, contraction was induced by electrical field stimulation (EFS, 0.5 msec duration and supramaximal voltage). Changing of the stimulation frequencies from resting 4 Hz to 0.4, 1 or 8 Hz caused typical negative staircase effect in twitch tension, but $^{45}Ca$ uptake showed bimodal increase. When the stimulation frequency was abruptly changed from 4 Hz to 0.4 Hz the atrial twitch tension showed three phased-enhancement, that is, the initial rapid increase (the first phase) followed by rapid decrease (the second phase) and stabilization (the third phase). $^{45}Ca$ uptake was equivalent to tension, i.e. initial significant increase in first 30 second and then decrease. Benzamil treatment abolished the first phase of increase in a dose dependent manner from $10^{-5}\;to\;3{\times}10^{-4}M.$ Bay k 8644 $(3{\times}10^{-5}M)$ treatment enhanced the inotropy induced by frequency reduction and abolished the second and third phase decreases. Benzamil treatment also suppressed the contraction stimulated by Bay K 8644. Although the contraction at 4 Hz stimulation was completely abolished by verapamil $3{\times}10^{-5}\;M$ pretreatment, the contraction reappeared as soon as the stimulation frequency was changed into 0.4 or 1 Hz and interstingly,$^{45}Ca$ uptake were significantly higher than no treatment. From these results, it is concluded that reduction of stimulation frequency causes calcium influx by the reverse mode Na-Ca exchange, resulting in initial rapid increase of twitch tension. then it turns into forward mode exchange to efflux the calcium, resulting in decrease of the twitch tension in left atria of rat.

• Korean Journal of Computational Design and Engineering
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• v.6 no.1
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• pp.48-58
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• 2001

One of the biggest problems of rapid prototyping(RP) technologies lies in their standard file format for CAD data exchange. Current methods using the de facto industry standard 'STL'have at times resulted in problems such as accuracy, redundancy, and integrity. In this paper we propose a STEP based data exchange framework for rapid prototyping systems. In this paradigm of data exchange, STEP models can be imported and converted into faceted B-rep. solid models for visualization and 2-D layer data for RP. Also an STL model, on the other hand, can be converted into a faceted B-rep. STEP model and exported as a new data exchange model with RP information.

• Journal of the Korean Geotechnical Society
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• v.30 no.7
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• pp.55-61
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• 2014

The need of geothermal energy is constantly increasing for economical and environmental utilization. Horizontal ground heat exchangers (GHEs) can reduce installation cost and increase efficiency. There are many kinds of GHEs, and it is known that slinky and spiral coil type GHEs show high thermal performance. Therefore, this paper presents experimental results of heat exchange rates in horizontal slinky and spiral coil type GHEs installed in a steel box whose size is $5m{\times}1m{\times}1m$. Dried Joomunjin standard sand was filled in a steel box, and thermal response tests (TRTs) were conducted for 30 hours to evaluate heat exchange rates by changing different pitch spaces of horizontal slinky and spiral coil type GHEs. As a result, spiral coil type GHE showed 30~40% higher heat exchange rates per pipe length than horizontal slinky type GHEs. Furthermore, long pitch interval (Pitch/Diameter=1) showed 200~250% higher heat exchange rates per pipe length than short pitch interval (Pitch/Diameter=0.2) in both spiral coil and horizontal slinky type GHEs, respectively.