• Title/Summary/Keyword: Exact test

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Specific Detection of Root Rot Pathogen, Cylindrocarpon destructans, Using Nested PCR from Ginseng Seedlings (Nested PCR 기법을 이용한 인삼 뿌리썩음병원균의 특이적 검출)

  • Jang, Chang-Soon;Lee, Jung-Ju;Kim, Sun-Ick;Song, Jeong-Young;Yoo, Sung-Joon;Kim, Hong-Gi
    • Research in Plant Disease
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    • v.11 no.1
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    • pp.48-55
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    • 2005
  • Cylindrocarpon destructans is a soil-borne plant pathogenic fungus causing root rot on ginseng and trees. Rapid and exact detection of this pathogen was practiced on ginseng seedlings by nested PCR using speciesspecific primer set. The second round of PCR amplification by Dest 1 and Dest 4 primer set formed 400 bp of species-specific fragment of C. destructans from the product of first round of amplification by ITS 1 and ITS 4 primer set. In the PCR sensitivity test based on DNA density, nested PCR detected to the limit of one fg and it meant the nested PCR could detect up to a few spores of C. destructans. Also, nested PCR made it possible to detect the pathogen from ginseng seedlings infected by replantation on artificial infested soil. Our nested PCR results using species-specific primer set could be utilized for diagnosis of root rot disease in ginseng cultivation.

A Numerical Method for Longitudinal Dispersion Equation for Nonconservative Contaminants (비보존성 오염물질에 대한 종확산 방정식의 수치해법)

  • Yu, Myeong-Gwan;Jeon, Gyeong-Su
    • Journal of Korea Water Resources Association
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    • v.32 no.6
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    • pp.607-616
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    • 1999
  • A fractional step finite difference model for the longitudinal dispersion of nonconservative contaminants is developed. It is based on splitting the longitudinal dispersion equation into a set of three equations each to be solved over a one-third time step. The fourth-order Holly-Preissmann scheme, an analytic solution, and the Crank-Nicholson scheme are used to solve the equations for the pure advection, the first-order decay, and the diffusion, respectively. To test the model, it is applied to simulate the longitudinal dispersion of continuous source released into a nonuniform flow field as well as the dispersion of an instantaneous source in a uniform flow field. The results are compared with the exact solution and those computed by an existing model. Compared to the existing model which uses Euler method for the first-order decay equation, the present model yield more accurate results as the decay coefficient increases.

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Nitrate Contents in Vegetables Cultivated by Organic Farming in Korea (우리나라 유기농 채소내 질산염 함량의 경시적 추이)

  • Sohn, Sang-Mok;Kim, Young-Ho;Yoon, Ji-Young
    • Korean Journal of Organic Agriculture
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    • v.7 no.2
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    • pp.125-151
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    • 1999
  • After the release of facts that the high nitrate contents in vegetables by Korean organic farming, organic farmers were recommended by Korean Organic Farmer Association to apply 20t/ha compost while they applied before as much as they could, sometimes even more than 100t/ha. This study was aimed to check the safety of Korean organic product in terms of nitrate content. Current nitrate content in leaf and root vegetables by 3 different farming systems(organic farming, conventional farming, greenhouse farming) were monitored and furthermore it was compared with previously published another data in Korea to find out any changes among the monitored years(1993-1999). Current nitrate content in chinese cabbage, lettuce and kale became less compare to those of the beginning of monitoring, and especially those cultivated organic farming got the lowest value while previous organic vegetables at beginning of 90's showed the higher value than those cultivated in greenhouse. This decrease in organic vegetables was affected by less application rate of organic fertilizer than before at 20t/ha. But the nitrate level by organic farming was still higher compare to those cultivated by conventional farming in open field, since currently monitored organic vegetables were cultivated basically in greenhouse condition. It shows the organic farmer the necessity of transfer their farming site and condition from greenhouse to open field in order to decrease of its high level of nitrate caused long-term application and slow release characteristics of organic fertilizer. It was concluded the adoption of soil nitrate test to recommend a organic farmer the exact application rate for need of crop growth. Additionally the mechanism of lower nitrate accumulation in rice and fruit vegetables were also discussed in the paper.

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Evaluation of Non-Watertight Dural Reconstruction with Collagen Matrix Onlay Graft in Posterior Fossa Surgery

  • Kshettry, Varun R.;Lobo, Bjorn;Lim, Joshua;Sade, Burak;Oya, Soichi;Lee, Joung H.
    • Journal of Korean Neurosurgical Society
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    • v.59 no.1
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    • pp.52-57
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    • 2016
  • Objective : Many surgeons advocate for watertight dural reconstruction after posterior fossa surgery given the significant risk of cerebrospinal fluid (CSF) leak. Little evidence exists for posterior fossa dural reconstruction utilizing monolayer collagen matrix onlay graft in a non-watertight fashion. Our objective was to report the results of using collagen matrix in a non-watertight fashion for posterior fossa dural reconstruction. Methods : We conducted a retrospective review of operations performed by the senior author from 2004-2011 identified collagen matrix (DuraGen) use in 84 posterior fossa operations. Wound complications such as CSF leak, infection, pseudomeningocele, and aseptic meningitis were noted. Fisher's exact test was performed to assess risk factor association with specific complications. Results : Incisional CSF leak rate was 8.3% and non-incisional CSF leak rate was 3.6%. Incidence of aseptic meningitis was 7.1% and all cases resolved with steroids alone. Incidence of palpable and symptomatic pseudomeningocele in follow-up was 10.7% and 3.6% respectively. Postoperative infection rate was 4.8%. Previous surgery was associated with pseudomeningocele development (p<0.05). Conclusion : When primary dural closure after posterior fossa surgery is undesirable or not feasible, non-watertight dural reconstruction with collagen matrix resulted in incisional CSF leak in 8.3%. Incidence of pseudomeningocele, aseptic meningitis, and wound infection were within acceptable range. Data from this study may be used to compare alternative methods of dural reconstruction in posterior fossa surgery.

Comparison of Root Images between Post-Myelographic Computed Tomography and Magnetic Resonance Imaging in Patients with Lumbar Radiculopathy

  • Park, Chun-Kun;Lee, Hong-Jae;Ryu, Kyeong-Sik
    • Journal of Korean Neurosurgical Society
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    • v.60 no.5
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    • pp.540-549
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    • 2017
  • Objective : To evaluate the diagnostic value of computed tomography-myelography (CTM) compared to that of magnetic resonance imaging (MRI) in patients with lumbar radiculopathy. Methods : The study included 91 patients presenting with radicular leg pain caused by herniated nucleus pulposus or lateral recess stenosis in the lumbar spine. The degree of nerve root compression on MRI and CTM was classified into four grades. The results of each imaging modality as assessed by two different observers were compared. Visual analog scale score for pain and electromyography result were the clinical parameters used to evaluate the relationships between clinical features and nerve root compression grades on both MRI and CTM. These relationships were quantified by calculating the receiver-operating characteristic curves, and the degree of relationship was compared between MRI and CTM. Results : McNemar's test revealed that the two diagnostic modalities did not show diagnostic concurrence (p<0.0001). Electromyography results did not correlate with grades on either MRI or CTM. The visual analog pain scale score results were correlated better with changes of the grades on CTM than those on MRI (p=0.0007). Conclusion : The present study demonstrates that CTM could better define the pathology of degenerative lumbar spine diseases with radiculopathy than MRI. CTM can be considered as a useful confirmative diagnostic tool when the exact cause of radicular pain in a patient with lumbar radiculopathy cannot be identified by using MRI. However, the invasiveness and potential complications of CTM are still considered to be pending questions to settle.

Validation of self-reported height and weight in fifth-grade Korean children

  • Lee, Bora;Chung, Sang-Jin;Lee, Soo-Kyung;Yoon, Jihyun
    • Nutrition Research and Practice
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    • v.7 no.4
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    • pp.326-329
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    • 2013
  • Height and weight are important indicators to calculate Body Mass Index (BMI); measuring height and weight directly is the most exact method to get this information. However, it is ineffective in terms of cost and time on large population samples. The aim of our study was to investigate the validity of self-reported height and weight data compared to our measured data in Korean children to predict obese status. Four hundred twenty-two fifth-grade (mean age $10.5{\pm}0.5$ years) children who had self-reported and measured height and weight data were final subjects for this study. Overweight/obese was defined as a BMI of or above the 85th percentile of the gender-specific BMI for age in the 2007 Korean National Growth Charts or a BMI of 25 or higher (underweight : < 5th, normal : ${\geq}5th$ to < 85th, overweight : ${\geq}85th$ to < 95th). The differences between self-reported and measured data were tested using paired t-test. Differences based on overweight/obese status were tested using analysis of variance (ANOVA) and linear trends. Pearson's correlation and Cohen's kappa were tested to examine agreements between the self-reported and measured data. Although measured and self-reported height, weight and BMI were significantly different and children tended to overreport their height and underreport their weight, the correlation between the two methods of height, weight and BMI were high (r = 0.956, 0.969, 0.932, respectively; all P < 0.001), and both genders reported their overweight/non-overweight status accurately (Cohen's kappa = 0.792, P < 0.001). Although there were differences between the self-reported and our measured methods, the self-reported weight and height was valid enough to classify overweight/obesity status correctly, especially in non-overweight/obese children. Due to bigger underestimation of weight and overestimation of height in obese children, however, we need to be aware that the self-reported anthropometric data were less accurate in overweight/obese children than in non-overweight/obese children.

Real Time Lip Reading System Implementation in Embedded Environment (임베디드 환경에서의 실시간 립리딩 시스템 구현)

  • Kim, Young-Un;Kang, Sun-Kyung;Jung, Sung-Tae
    • The KIPS Transactions:PartB
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    • v.17B no.3
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    • pp.227-232
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    • 2010
  • This paper proposes the real time lip reading method in the embedded environment. The embedded environment has the limited sources to use compared to existing PC environment, so it is hard to drive the lip reading system with existing PC environment in the embedded environment in real time. To solve the problem, this paper suggests detection methods of lip region, feature extraction of lips, and awareness methods of phonetic words suitable to the embedded environment. First, it detects the face region by using face color information to find out the accurate lip region and then detects the exact lip region by finding the position of both eyes from the detected face region and using the geometric relations. To detect strong features of lighting variables by the changing surroundings, histogram matching, lip folding, and RASTA filter were applied, and the properties extracted by using the principal component analysis(PCA) were used for recognition. The result of the test has shown the processing speed between 1.15 and 2.35 sec. according to vocalizations in the embedded environment of CPU 806Mhz, RAM 128MB specifications and obtained 77% of recognition as 139 among 180 words were recognized.

Development Portable Pipe Spool Location-Confirm System Based UHF RFID (UHF RFID기반 이동형 파이프 스풀 위치 인식 시스템 개발)

  • Kim, Jinsuk;Shin, Yongtae
    • KIPS Transactions on Computer and Communication Systems
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    • v.3 no.10
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    • pp.329-336
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    • 2014
  • Pipe spool is the most important element of plant equipment in plant construction site. Currently, the plant construction site manage the pipe spool location and usage history by handwriting. Frequently, the pipe spool is moved to unpredictable places by field workers in many construction sites and in cases like this, the pipe stool is replaced with another similar pipe spool. Since it's hard to determine the exact location of some of the pipe spools, it takes unnecessary time and labour to find the missing pipe spools, which proves that stock management is not under control. The purpose of our system is to make the identification of real-time location of the field pipes possible by attaching UHF RFID tags to the pipe spool, which will be used to connect with UHF RFID reader and antenna on vehicles. A field test conducted by the proposed system resulted in the success rate of 98% and the missing 2% was recuperated by hands-on correction, which proved that stock management through the proposed method can be 100% effective. The proposed system is expected to reduce labour costs and make stock control of assets possible, as well as applicable to similar stock management environments.

An Analysis on the types of Children등s Conception on Volcanos and Earthquakes in the Elementary Schools (화산과 지진에 관한 초등학교 학생들의 개념 유형 분석)

  • Wooi, Hong;Lee, Hang-Ro;Woo, Jong-Ok
    • Journal of Korean Elementary Science Education
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    • v.17 no.1
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    • pp.33-46
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    • 1998
  • This study researched the concepts that elementary school students have after having learned volcano and earthquake. The reacher collected and adjusted the journals related to this study, and sampled the concepts included in Chap. One "Moving Earth", the first term of the elementary school sixth year. The students' descriptive responses were analyzed after subjective questions on the basis of above were developed and put into. After the contents described in the answer sheets were categorized by key words and the types of concept were made out by qualitative analysis, it was showed what was differences between the sexual, regional and achievement levels. The results of this study were as follows; first, though the types of concept about volcano and earthquake were found variably, it was showed that wrong preconception was not corrected, especially the exact understanding about terms not attained to, which led to the trouble in conceptual readjustment of students. Second, though the response rate about the types of scientific concept was found high that volcano was made out of explosion and eruption of magma and lava, there were also children who described not the interior factor of globe but the surface factor. Third, according to the results divided into the interior factor of globe and the surface factor and analyzed about earthquake, there were a great number of children who responded as the surface factor though most of the students responded to the interior factor of globe. Fourth, through some types of concept, it was found that the formation of concept was much influenced by experience of children's life, curriculum, and the method of study. Fifth, In the most test question, the scientific concept was found that boys were higher than girls in the distinction of sex, the children of cities than those of town and island, and the upper group than the lower group in the achievement levels.

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A SAR Signal Processing Algorithm using Wavenumber Domain

  • Won, Joong-Sun;Yoo, Hong-Ryong;Moon, Wooil-M.
    • Korean Journal of Remote Sensing
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    • v.10 no.2
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    • pp.1-15
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    • 1994
  • Since Seasat SAR mission in 1978, SAR has become one of the most important surface imaging tools in satellite remote sensing SAR achieves high resolution by signal processing synthesizing a larger aperture. Therefore, SAR signal processing along with antenna technology has been centered upon SAR technologies. Thus interpreters of SAR imagery as well as those who involved in signal processing require the knowledge of the principal SAR processing algorithm. Although the conventional range-Doppler approach has been widely adopted by many SAR processors, azimuth compression including the range migration has been problematic. The recent development of the wavenumber domain approace is able to provide high precision SAR focusing algorithm. Compared with the wavenumber domain algorithm derived by applying Born (first) approximation, the transfer function of the conventional range-Doppler algorithm accounts only for the first order approximation of the exact transfer function. The results of a simulation and an actual test using airborne C-band SAR configuration demonstrate the dxcellent performance of the wavenumber domain algorithm.