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http://dx.doi.org/10.5423/RPD.2005.11.1.048

Specific Detection of Root Rot Pathogen, Cylindrocarpon destructans, Using Nested PCR from Ginseng Seedlings  

Jang, Chang-Soon (Department of Agricultural Biology, Chungnam National University)
Lee, Jung-Ju (Department of Agricultural Biology, Chungnam National University)
Kim, Sun-Ick (Geumsan Agricultural Development & Technology Center)
Song, Jeong-Young (Department of Agricultural Biology, Chungnam National University)
Yoo, Sung-Joon (Bioshield Co. Ltd. HTVC KAIST)
Kim, Hong-Gi (Department of Agricultural Biology, Chungnam National University)
Publication Information
Research in Plant Disease / v.11, no.1, 2005 , pp. 48-55 More about this Journal
Abstract
Cylindrocarpon destructans is a soil-borne plant pathogenic fungus causing root rot on ginseng and trees. Rapid and exact detection of this pathogen was practiced on ginseng seedlings by nested PCR using speciesspecific primer set. The second round of PCR amplification by Dest 1 and Dest 4 primer set formed 400 bp of species-specific fragment of C. destructans from the product of first round of amplification by ITS 1 and ITS 4 primer set. In the PCR sensitivity test based on DNA density, nested PCR detected to the limit of one fg and it meant the nested PCR could detect up to a few spores of C. destructans. Also, nested PCR made it possible to detect the pathogen from ginseng seedlings infected by replantation on artificial infested soil. Our nested PCR results using species-specific primer set could be utilized for diagnosis of root rot disease in ginseng cultivation.
Keywords
Cylindrocarpon destructans; Diagnosis; Ginseng; Nested PCR; Root rot;
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