• 제목/요약/키워드: Evodia fruit

검색결과 12건 처리시간 0.023초

Cyclooxygenase-2 Inhibitor from Evodia rutaecarpa

  • Kang, Sam-Sik;Kim, Ju-Sun;Son, Kun-Ho;Kim, Hyun-Pyo;Chang, Hyeun-Wook
    • Natural Product Sciences
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    • 제5권2호
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    • pp.65-69
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    • 1999
  • By bioassay guided fractionation followed by chromatographic separation of the MeOH extract from the fruit of Evodia rutaecarpa (Juss.) Benth. (Rutaceae), a new cyclooxygenase-2 inhibitor was isolated and identified as an alkaloid, rutaecarpine. Other alkaloids such as evodiamine and dehydroevodiamine together with limonoids were also isolated and characterized.

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Isolation and Determination of Alkaloids in Evodiae Fructus by forming Complex Compound (착 화합물 생성에 의한 오수유 중 알칼로이드의 분리와 정량)

  • Kim, Sung-Eun;Kim, Dae-Keun;Shin, Tae-Yong;Lim, Jong-Pil;Eom, Dong-Ok
    • Korean Journal of Pharmacognosy
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    • 제34권3호통권134호
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    • pp.206-209
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    • 2003
  • The fruit of Evodia officinalis (Rutaceae) is known for containing a number of indoloquinazoline and quinoline type alkaloids. Evodiamine, evocarpine and rutaecarpine are the major constituents of Evodiae Fructus. These alkaloids were isolated and determined by forming complex compounds from Evodiea Fructus. For the determination of these alkaloids, a new spectrophotometric method was developed with a simple and selective sample clean-up using thiocyanatocobaltate [II] complex ion. The absorbance of alkaloidal complex in 1.2-dichloroethane solution was measured at 625 nm. A calibration curve for the alkaloids isolated from Evodia Fruit was linear over the concentration range of $1.0{\sim}6.0\;mg/ml$. The method proved to be rapid, simple and reliable for the isolation and the determination of the alkaloids in Evodiae Fructus.

Studies on the Components of Evodia daniellii $H_{EMSLEY}$ in Korea (한국산 오수유성분에 관한 연구)

  • Chung, Bo-Sup
    • Korean Journal of Pharmacognosy
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    • 제1권4호
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    • pp.119-124
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    • 1970
  • Evodia daniellii $H_{EMSLEY}$, a plant belonging to Rutaceae, is cultivated in Korea and used as a folkmedicine for gastric inflammation, extermination of noxious insects, and headache. The seed oil of this plant also has been used in various diseases, for example, dermatitis, scabies and so forth. From the barks, fruit peels, and seed oil of Evodia daniellii $H_{EMSLEY}$, four crystalline compounds were isolated. Three compounds except one were characterized as methyl sinapate, bergapten, and evodiamine by m.p. determination, elemental analysis, UV, IR, NMR spectra and mass analysis. Even though methyl sinapate was a known synthetic compound, it was not yet reported as a natural product. From the seed oil, unsaponifiable fraction was separated and was analyzed. It was considered to be consisted with sterols, hydrocarbons and tocopherols.

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Studies on the Processing of Crude Drugs(VIII): The Changes of Physico-chemical Parameter and Biological Activities by Processing of Evodia Fruit (한약수치에 관한 연구(제 8보): 오수유 탕포법)

  • Park, Sung-Hwan;Lee, Woo-Jung;Choi, Hyuck-Jai;Song, Bo-Whan;Kim, Dong-Hyun;Kim, Nam-Jae
    • Korean Journal of Pharmacognosy
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    • 제36권2호통권141호
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    • pp.102-108
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    • 2005
  • We have studied the physico-chemical change and pharmacological transformation of traditional herbal medicines with processing. Evodia fruit (EF) has processed for the purpose of detoxification and reduction of its bitter taste. There are various methods of processing EF in Oriental medicinal reference. Among them, we processed EF according to the method of Sang-han theory, the most famous medicinal reference. We processed EF with washing in hot water, and then dried. Processed EFs(PEFs) were prepared according to the above procedure through repetition of 1,3,5 and 7 times respectively. The contents of weight loss, water extract, diluted ethanol extract, ether extract, total ash, acid insoluble ash, alkaloids and limonin in non-processed EF (NPEF) and PEFs were examined. The weight loss, and contents of water extract and dilute ethanol extract in PEF showed decrease in proportion to increase of washing time, while the contents of ether extract, total ash and acid insoluble ash showed little change as compared with those of NPEF. And the content of evodiamine and rutaecarpine was not changed distinctly. However, the content of limonin decreased in the final processed material in proportion to increase of washing times with hot water. And the intense of bitter taste in PEF was also reduced. These results were ascribed to the flowing-out of the water-soluble portion. The biological activities of NPEF and PEF were also investigated. In the test of DPPH scavenging effect, xanthine oxidase inhibition effect and TBA-Rs effect, PEF was more effective than NPEF in vitro. Also, both NPEF and PEF showed potent analgesic and anti-inflammatory effects against in mice. Especially, PEF by 3 times washing with hot water was more effective than other PEFs. From these results, it is considered that PEF should be prepared by 3 times washing with hot water.

A study on the fruit color characteristics and persisting period of deciduous woody landscape plants in Middle area of Korea (중부지방 낙엽조경수목의 열매색 특성 및 지속 기간에 관한 연구)

  • 서병기;심경구;정해준;심재성
    • Journal of the Korean Institute of Landscape Architecture
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    • 제23권3호
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    • pp.144-154
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    • 1995
  • The fruit color characteristics and persisting period of deciduous woody landscape plants were investigated through the field survey in Suwon region from January 1, 1992 to March 20, 1993. The summary of the study results was as follows; The total fruit color persisting period was about 320 days from May 1, 1992 when Prunus mume was beginning of fruit coloring, to March 20, 1993 when the fruits of Platanus occidentalis and platanus X acerifolia were persisting. And the plants of fruit persisting period over 60 days after leaf falling were Vibumum erosum, Ilex serrata, Ilex verticillata 'Christmas Cheer', Platanus X acerifolia, Platanus occidentalis, Berberis thunbergii 'Atropupurea', Ligustrum obtusifolium. According to the KBS standard color number, 52.6% of of the fruit color were red, 18.9% yellow, 11.6% black, green 9.5%, white 2.1%, violet 1.1%, and red is followed black 4.2%. Evodia daniellii, Ilex verticillata 'Christmas Cheer', Ilex serrata, Ginkgo biloba, Lindera obtusiloba, and Lindera erythrocarpa should be planted male and female species together for fruits. We got the new information on the fruit color characteritics and persisting period of Malus 'Hopa', Malus 'Almey', Malus 'Pioneer X', Acer rubrum, Malus prunifolia, Pyrus serotina, Berberis thunbergii 'Atropurpurea', Vibumum rhytidophyllum, Rosa spp. Vitis vinifera, Vaccinium angustifolium, Ilex verticillata 'Christmas Cheer', Magnolia stellata, Aronia arbutifolia, Sorbus alnifolia, Lonicera japonica var. aueroreticulta, and Ligustrum X vicaryi. And we need to introduce new cultivars of woody landscape plants including Malus spp, Berberis spp, Sorbus alnifolia yellow autumn leaf clone etc. for the better planting design. The fruit persisting period of woody landscape plants studied by fruit name was that sorosis 276 days, samara 155 days, legume 153 days, hip 133 days, pome was 124 days, drupe 92 days, berry 73 days, capsule 67 days, follicle 55 days and nut 52 days respectively.

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Effect of Methanol Extract Concentration on the Anti-oxidative Activity and Toxicity of Evodiae Fructus to AGS Cells (오수유의 메탄올 추출 농도에 따른 항산화와 AGS세포에 대한 독성 효과)

  • Yang, Ji Yeong;Byeon, Hwiyong;Kim, Jin Woo;Kim, Sa Hyun;Lee, Pyeongjae
    • Journal of the Korean Society of Food Culture
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    • 제35권4호
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    • pp.400-405
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    • 2020
  • Evodiae Fructus is the dried unripe fruit of Evodia rutaecarpa, and has traditionally been used for treating stomachache and diarrhea. Evodiamine and rutaecarpine, the major biologically active compounds of Evodiae Fructus, are reported to have anti-oxidative and anti-inflammatory effects, as well as inhibit proliferation and metastasis of various cancer cells. The current study investigates the anti-oxidative and anti-cancer effects of the Evodiae Fructus extract, considering varying concentrations of methanol extraction (40, 80, and 95%). High contents of total phenolic compounds were determined in the order of extracts 80, 95, and 40%. Evaluating contents of the 95, 80, and 40% extracts revealed 36.77, 7.29, and 1.86 ㎍/mg evodiamine, respectively, and 53.02, 17.16, and 3.79 ㎍/mg rutaecarpine, respectively, with the highest content of both compounds obtained in the 95% extract. DPPH radical scavenging activity was observed to be inversely proportional to the contents of total phenolic compounds, with decreasing SC50 values obtained in the order 80, 95, and 40% extract. The 95 and 80% extracts exerted toxicity to AGS gastric cancer cells, but the 40% extract was non-toxic. Evodiamine is a known anti-cancer agent, and could be responsible for the observed toxicity. Cleavage of PARP, and Caspase-3, -7, -8 and -9 was observed in the 95% extract-treated AGS cells, indicating that cell toxicity exerted by the 95% extract could be attributed to apoptosis.

Metabolism of Rutaecarpine by Rat Liver Microsomes

  • Lee, Sang-Kyu;Lee, Jae-Ick;Jahng, Young-Dong;Chang, Hyeun-Wook;Lee, Eung-Seok;Kim, Dong-Hyun;Jeong, Tae-Cheon
    • Proceedings of the PSK Conference
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    • 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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    • pp.127.2-128
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    • 2003
  • Rutaecarpine is an alkaloid originally isolated from the unripe fruit of Evodia rutaecarpa. In addition to its traditional use in treatment of gastrointestinal disorders, rutaecarpine has recently been characterized to have anti-inflammatory activity through cyclooxygenase-2 inhibition. More recently, to develop rutaecarpine as an anti-inflammatory agent, total synthesis of rutaecarpine has successfully been established in our group. (omitted)

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Cytotoxic Activity of Medicinal Plant Extracts against Human Tumor Cell Lines

  • Jeong, In-Hong;Lee, Jeong-Ock;Kim, Choul-Soo;Kim, Soo-Un;Ahn, Young-Joon
    • Journal of Applied Biological Chemistry
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    • 제43권1호
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    • pp.59-61
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    • 2000
  • The cytotoxic activities of the methanol extracts of 44 plant species in 31 families against five human solid A549 (lung), SK-OV-2 (ovarian), SK-MEL-2 (melanoma), XF-498 (central nervous system), and HCT-15 (colon) tumor cell lines were examined using the sulforhodamine B (SRB) assay. Responses varied with both cell line and plant species used. Potent cytotoxic activities ($ED_{50}$, <$40{\mu}g/ml$) against all model tumor cell lines were produced from the extracts of Rhus chinensis gall (Galla rhois), Betula platyphylla var. japonica bark, Inula helenium root, Cinnamomum cassia bark, Cinnamomum sieboldii root bark, Lysimachia davurica whole plant, and Evodia rutaecarpa fruit. These plants may be useful for developing new types of naturally occurring anti-tumor agents.

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Effects of Oral Rutaecarpine on the Pharmacokinetics of Intravenous Chlorzoxazone in Rats

  • Bista, Sudeep R.;Lee, Sang-Kyu;Thapa, Dinesh;Kang, Mi-Jeong;Seo, Young-Min;Kim, Ju-Hyun;Kim, Dong-Hyeon;Jahng, Yurng-Dong;Kim, Jung-Ae;Jeong, Tae-Cheon
    • Toxicological Research
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    • 제24권3호
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    • pp.195-199
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    • 2008
  • It has been reported that hepatic microsomal cytochrome P450(CYP) 2E1 is responsible for the metabolism of chlorzoxazone(CZX) to 6-hydroxychlorzoxazone. The present study was undertaken to assess the possible interaction of rutaecarpine, an alkaloid originally isolated from the unripe fruit of Evodia rutaecarpa, with CZX. Male Spraque-Dawley rats were administered with 80 mg/kg/day of oral rutaecarpine for three consecutive days. Twenty four hr after the pre-treatment with rutaecarpine, the rats were treated with 20 mg/kg of intravenous CZX. Rat hepatic microsomes isolated from rutaecarpine-treated rats showed greater(50% increase) activity of p-nitrophenol hydroxylase(a marker of CYP2E1) when compared with the control rats. Compared with control rats, the AUC of CZX was significantly smaller(84% decrease) possibly due to significantly faster CL(646% increase) in rats pretreated with rutaecarpine. This could be, at least partially, due to induction of CYP2E1 by rutaecarpine.

Effects of Rutaecarpine on the Pharmacokinetics of Caffeine and Its Three Metabolites in Rats

  • Seo, Young-Min;Noh, Keum-Han;Kong, Min-Jeong;Lee, Dae-Hun;Kang, Mi-Jeong;Jahng, Yurng-Dong;Kang, Won-Ku;Jeong, Byeong-Seon;Jeong, Tae-Cheon
    • Biomolecules & Therapeutics
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    • 제19권2호
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    • pp.243-247
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    • 2011
  • Rutaecarpine, an alkaloid originally isolated from the unripe fruit of Evodia rutaecarpa, has been shown to be anti-inflammatory. In the present study, a possible interaction between rutaecarpine and caffeine was investigated in male Sprague Dawley rats. Twenty four hr after the oral pretreatment with rutaecarpine at 80 mg/kg for three consecutive days, rats were treated intravenously with 10 mg/kg of caffeine. Compared with control rats, the pharmacokinetic parameters of caffeine in rutaecarpine-pretreated rats were significantly changed, possibly due to the rapid metabolism. The production of three metabolites of caffeine (i.e., paraxanthine, theobromine and theophylline) was also significantly changed in rats pretreated with rutaecarpine. The present results suggest that oral rutaecarpine would change the intravenous pharmacokinetic characteristics of caffeine.