• Proceedings of the Korean Institute of Landscape Architecture Conference
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• 1999.03a
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• pp.42-43
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• 1999

• Journal of Veterinary Science
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• v.25 no.4
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• pp.52.1-52.10
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• 2024

Importance: Kidney disease is prevalent among veterinary species, including zoo animals; however, investigations into this condition in striped skunks (Mephitis mephitis) are scarce. Diagnostic tools for kidney diseases in this species also remain limited. Objective: This study aimed to assess the utility of symmetric dimethylarginine as a biomarker for kidney disease in captive striped skunks in Korea. Methods: This retrospective study analysed 11 striped skunks housed at the Everland Zoo between 2017 and 2021. Blood samples were collected during health checks. Kidney function was assessed through blood analysis and diagnostic ultrasound, with necropsies conducted on deceased animals. Symmetric dimethylarginine levels were measured in 27 plasma samples collected from 11 skunks. Results: Over the study period, seven skunks were diagnosed with kidney disease. Analysis of 27 blood samples revealed a concurrent increase in SDMA levels with concentrations of blood urea nitrogen and blood creatinine. In 3 of the 7 skunks with kidney disease, symmetric dimethylarginine exceeded 14 ㎍/dL prior to the elevation of blood urea nitrogen and blood creatinine above the upper reference limit. Conclusions and Relevance: To our knowledge, this is the first study investigating symmetric dimethylarginine in captive striped skunks in Korea. Our findings suggest that symmetric dimethylarginine may serve as an early and consistent biomarker for renal dysfunction in striped skunks. Further studies with larger clinical sample size from striped skunks are needed to validate the clinical utility of blood symmetric dimethylarginine concentration.

• Asian Journal of Turfgrass Science
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• v.18 no.4
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• pp.211-219
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• 2004

Transgenic zoysiagrass (Zoysia japonica cv. Zenith) plants have been obtained by particle bombardment of embryogenic callus with the plasmid pSMABuba, which contains hygromycin resistance (hpt) and bialaphos resistance (bar) genes. Parameters on DNA delivery efficiency of the particle bombardment were partially optimized using transient expression assay of a chimeric $\beta-glucuronidase$(gusA) gene driven by the CaMV 35S promoter. Stably transfarmed zoysiagrass plants were recovered with a selection scheme using hygromycin. Transgenic zoysiagrass plants were confirmed by PCR analysis with specific primer for bar gene. Expression of the transgene in transformed zoysiagrass plants was demonstrated by Reverse transcriptase (RT)-PCR analysis. All the tested transgenic plants showed herbicide BastaR resistance at the field application rate of $0.1\%-0.3\%$.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.2 no.4
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• pp.64-73
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• 1999

This study was conducted to find out the effects of hydroseeding material and seed mixture on the revegetation of rock cut-slopes by PEC4 (Polymer-Ecology-Control) Hydroseeding Measures. PEC4 hydroseeding material was applied to four cut-slopes using hydroseeding measures from April to August, 1999, and the field survey was carried out by monthly. PEC4 material consisted of bark compost and organic soil amendments. This material has high content of organic matter and high level of water holding capacity. PEC4 hydroseeding material shows low level of soil hardness, so it gives to good condition for seed germinating and plant growing in early stage. PEC4 material attached at rock cut-slopes by two types of adhesive agent was not eroded by rainfall. The plant coverage and number of plant species were affected by mixing ratio of seeds and seeding timing. From the viewpoint of plant establishment, the optimal hydroseeding timing of mixed seeds for plant growth seems to be in May. Most of the plant seeds were germinated well and they covered rock cut-slopes so quickly and effectively. Plant importance value of Silene armeria and Platycodon grandiflorum. were higher than any other seeded-native species in the competition between native species and exotic species, so they have enough possibility to be used for slope revegetation works. Thus it leads to conclusion that the revegetation method used in this experiment was a very effective method for plant establishment on rock cut-slopes.

• Korean Journal of Veterinary Pathology
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• v.2 no.2
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• pp.139-145
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• 1998

A total of 5 animals including 3 raccoons, 1 badger, and 1 fennec fox kept in outdoor exhibits at the Everland Zoological Gardens showed depression, anorexia, dyspnea, serous oculonasal discharge, diarrhea, and convulsions. All the affected animals died within 10 days after the onset of clinical signs. This outbreak lasted about 4 months. On necropsy, major gross lesions were confined to the lungs. Red to grey sublobular to lobular consolidations with various sized tan to reddish spots were observed in the lungs. Histopathologically, the pulmonary lesions were characterized by acute to subacute bronchointerstitial pneumonia with secondary bacterial or adenoviral infections. Intracytoplasmic eosinophilic inclusion bodies compatible with canine distemper virus (CDV) were found in the lung, urinary bladder, kidney, intrahepatic bile duct, stomach, small and large intestines. Multifocal areas of severe demyelination and accumulation of gitter cells or nonsuppurative inflammation were seen in the brains of 2 raccoons. CDV -specific antigens were demonstrated in the lung sections on immunofluorescent assay. The present report describes an outbreak of CDV infection in a zoo and indicates the range of susceptible zoo animal species.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.3 no.4
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• pp.33-42
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• 2000

This study is carried out to investigate plant community structure, actual vegetation map and flora for examining the relationship between naturally introduced vegetation and surrounding vegetation in Hamtae coal-mined spoil lands. According to the analysis of vegetation, surrounding vegetation was divided into five plant community groups : Pinus koraiensis community, Pinus densiflora - Quercus mongolica community, Q. mongolica - Deciduous broadleaved trees community, Betula schmidtii - B. davurica community, Larix leptolepis community. Robinia pseudo-acacia, which had been planted, was dominant species in coal-mined spoil lands. Young trees, which were naturally introduced, such as B. schmidtii, B. davurica etc., partially appeared with the high density($15{\sim}19ea/100m^2$) in coal-mined spoil lands. The flora of this area was listed as 54 taxa; 22 families, 42 genus, 50 species, 3 varieties and 1 forma. Especially Spodiopogon sibiricus, Miscanthus sinensis, Arundinella hirta, Artemisia capillaris, Artemisia iwayomogi and Youngia denticulata appeared with the high frequency. It is estimated that the proper way for harmonious and effective revegetation is to target B. schmidtii - B. davurica communities and to seed B. schmidtii - B. davurica mixing highly frequent herbaceous species.

• Korean Journal of Veterinary Pathology
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• v.3 no.1
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• pp.55-59
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• 1999

Metastatic squamous cell carcinoma was described in two female Pere David's deer reared at Everland zoo. Both deer with chronic emaciation had the mass on right maxilla(9$\times$11$\times$15cm or 20$\times$17cm) which was composed of miliary tan creamy contents and encapsulated by connective tissue. The undulating contents in the mass was a1so extended in the underlying or adjacent soft palate, maxillary and frontal sinus causing severe bone destruction. In one deer, two fistula were also noted in the right periocular area. Histologically, the neoplastic masses of both deer consist of anastomosing cords or nests of squamous epithelial cells with intercellular bridge or keratin pearl formation. The neoplastic cells invade deep into the subcutis and bony tissue. Mitotic figure was rare. Multifocal areas of necrosis and hemorrhages were also noted in the dermis. Metastasis to maxilla and ethmoid bones and/or to submandibular lymph node was noted in both cases. The diagnosis was based on the results of histopathology.

• Journal of Veterinary Clinics
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• v.26 no.5
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• pp.511-513
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• 2009

This report deals with a 3-year-old male Yellow-crowned Amazon (Amazona orcrocephala) that died after twenty five days history of cloacal prolapse. On necropsy, multiple to occasionally coalescing well-demarcated tan nodules were scattered throughout the entire liver lobes. The nodules were firm and slightly elevated from the surface. The neoplastic nodules were also recognized on cut sections of the liver. Histologically, the nepatic nodules consisted of cuboidal to low columnar cells forming tubular to acinar pattern with marked desmoplasia. The neoplastic cells had small to moderate amounts of eosinophilic cytoplasm and round nucleus with one to two prominent nucleoli. Based on these results, hepatic nodules was diagnosed as primary cholangiocellular carcinoma.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.3 no.4
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• pp.43-51
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• 2000

This study was carried out to develop technologies of restoration and revegetation through monitoring the change of vegetation after setting up 3 type experimental sites on abandoned coal-mine lands. According to the results of 7 month's monitoring, sown species and a variety of invading species appeared at experimental sites. Moreover, the number of species, individuals and the ratio of coverage increased as time passed on. After 5 months, each experiment sites showed the result of successful revegetation. The most effective sites was treated by overspreading 1 cm loam soil on coal minesoil. As a result of this study, it is considered that the revegetation of abandoned coal-mine lands can be achieved effectively by making the minimum of foundation layer and improving the efficiency through mechanized construction. Besides, it is recommended to use native and early stage-succession species adapting themselves to coal minesoil, such as Gramineae, Leguminosae, Compositae and etc.

• Journal of Veterinary Clinics
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• v.22 no.3
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• pp.249-252
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• 2005

This study was carried out to assess hip joint in Labrador retrievers. From 1999 to 2004, 131 Labrador retrievers were tested by British Veterinary Association(BVA)/Kennel club(KC) scoring scheme, 66 were tested by $PennHIP^{(R)}$ method. In this study the mean score by BVA/KC scoring scheme was $12.5{\pm}6.6$ and the mean DI was $0.53{\pm}0.14$. It was found that Labrador retrievers of Samsung Guide Dog school have better hip joint condition than that of Labrador retrievers registered in BVA, and have similar mean DI to PennHIP breed mean score. This study suggested that using both BVA/KC scoring scheme and $PennHIP^{(R)}$ method was a specific method which can make the most possible definitive diagnosis, could provide baseline hip information for the hip joint in Labrador retrievers as Guide Dog.