• 한국임상수의학회지
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• 제24권2호
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• pp.264-268
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• 2007

Three German Shepherd dogs (5 years old, 30 kg, male; 4 years old, 23 kg, female; 4 years old, 24 kg, female) with history of acute vomiting, hemorrhagic diarrhea, anorexia and dullness were admitted to veterinary clinic in the military working dogs training corp (MWDTC). All of them showed same symptoms after ingestion the $'PREVNTIC^{(R)}'$ collar to control ecto-parasites infection in field. One dog was operated by gastrotomy and the $5{\sim}6$ pieces of the $'PREVNTIC^{(R)}'$ collar were removed. The dog was completely cured by intensive care within 2 hours after $'PREVNTIC^{(R)}'$ collar ingestion. However, two dogs were operated by gastrotomy and intensive care over 24 hours after $'PREVNTIC^{(R)}'$ collar ingestion but died. In autopsy, the $5{\sim}6$ pieces of hard materials were found in the stomach, and severe thickness, congestion hemorrhage and swelling of the stomach walls and mucous membrane were observed. In these cases, it was suggested that the first aid of treatment such as gastrotomy within two hours after $'PREVNTIC^{(R)}'$ collar ingestion as soon as possible was helpful for attenuating of toxicosis.

• Parasites, Hosts and Diseases
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• 제59권5호
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• pp.465-471
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• 2021

Culicoides biting midges (Diptera: Ceratopogonidae) are hematophagous arthropod vectors that transmit epizootic arthropod-borne viruses (arboviruses). Arboviruses are recognized as causes of pregnancy loss, encephalomyelitis, and congenital malformations in ruminants. Therefore, continuous monitoring and control of Culicoides, which causes significant damage to industrial animals are necessary. We performed attraction and repellent tests in Culicoides using various essential oils, cow dung, and carbon dioxide (CO₂). Culicoides tended to move more to cow dung (60.8%, P<0.0001) and CO₂ (63.8%, P<0.01). To the essential oils as repellents, 26.1% (P<0.0001), 18.7% (P<0.001), and 25.5% (P<0.01) of the Culicoides moved to the lavender, lemongrass, and eucalyptus chamber, respectively. The Culicoides that moved to the 3 essential oils chambers showed markedly low activity. Collectively, it was showed that Culicoides tended to be attractive to cow dung and CO₂, and repellent from the 3 essential oils.

• 대한통합의학회지
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• 제10권4호
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• pp.103-111
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• 2022

Purpose : In recent years, essential oils have been produced using natural extracts for various uses. Their functionality is currently being tested not only for cosmetics and perfumes but also for other categories of products. Therefore, this study verified their antibacterial effects on S. mutans, P. gingivalis, and L. gasseri which are the representative strains that cause oral diseases. Methods : Eighteen types of natural essential oils were made at a concentration of 50 % (v/v) using Tween 20, and their antibacterial effects were verified by applying S. mutans, P. gingivalis, and L. gasseri. The antibacterial effects were measured with the disc diffusion method. All the experiments were repeated three times, and the mean value of three measurement values for each variable was used for data analysis. A one-way analysis of variance was conducted using these mean values. Results : Of the eighteen types of essential oils tested, sixteen types showed antibacterial effects on S. mutans, and sixteen and fifteen types exhibited antibacterial effects on P. gingivalis and L. gasseri respectively. The types of essential oils with high-level antibacterial activities were geranium, may chang, and bergamot for S. mutans, lemongrass, bergamot, and eucalyptus for P. gingivalis, and lemongrass, machan, and geranium for L. gasseri in order of antibacterial effect. This result was statistically significant (p<.001). In addition, in the case of mandarin oil, it was found that there was no antibacterial activity in all three strains. Conclusion : This study proved the antibacterial activities of essential oils, which are used for various purposes in daily life, against dental caries and periodontal diseases. The study results will likely be applied to different prevention programs for oral health and broadly used to develop products such as oral care items and dentifrices.

• 한국미생물·생명공학회지
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• 제38권1호
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• pp.93-104
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• 2010

프랑킨센스 에센셜 오일(BSEO)이 알러지성 천식 생쥐 모델의 폐, BALF 및 PBMC 내 면역세포 변화에 미치는 영향을 조사하였다. 본 연구는 Balb/c 생쥐를 이용하여 실험 1일에 $100\;{\mu}g$ ovalbumin (OVA)을 복강 내 투여하고, 실험 14일에 $50\;{\mu}g$ OVA를 기도 내 투여하여 감작시킨 후 OVA를 반복 흡입시켜 천식을 유발하였다. 천식 유발 생쥐군에 0.3% BSEO를 8주간, 1주에 3회, 1회에 30분간 nebulizer로 흡입시켰고, 실험일로부터 총 12주 후에 살처분시켜 폐, 기관지 폐포 세척액 (bronchoalveolar lavage fluid; BALF), 말초혈액단핵세포 (peripheral blood mononuc1ear cell; PBMC)를 분리하여 조직 내의 면역세포 변화를 관찰하였다. 본 연구 결과 알러지성 천식 유발 생쥐에서 기관지 수축제에 대한 기도 과민성반응이 천연 아로마 에센셜 오일군인 Bergamot군, Eucalyptus군, Chamomile군, Marjoram군, 및 Frankincense군 중 Frankincense군에서 효과적으로 억제됨을 확인하였다. 또한 알러지성 천식유발로 증가한 폐무게, 총폐포수, BALF수, MNL, DLN수가 억제됨을 확인하였다. BSEO는 알러지성 천적 모델 생쥐의 폐 조직 내의 $CD3e^+/CD19^-$, $B220^+/CD23^+$, $CD11b^+/Gr-1^+$ 세포 비율을 억제하였으며 BALF 내의 $CD4^+/CD8^-$, $B220^+/CD23^+$, $CD3e^+/CCR3^+$ 세포비율을 억제하였다. 또한 PBMC내 $CD3e^+/CD19^-$, $CD3^+/CD69^+$, $B220^+/CD23^+$ 세포비율을 억제하였다. 이러한 결과는 BSEO가 각 조직내의 면역세포의 활성 억제를 통한 면역조절작용으로 알러지성 천식의 치료에 효과적일 것으로 사료되었다.