• 한국응용곤충학회지
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• 제45권2호
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• pp.189-194
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• 2006

털두꺼비하늘소(Moechotypa diphysis) 성충에 대한 18종 식물정유의 훈증독성을 조사하였다. 그들 중 10 $\mu\ell/\ell$ (공기)의 농도에서 eucalyptus oil, rosemary oil, 그리고 pennyroyal oil은 100%, sage oil은 85%의 살충활성을 나타내었다. 4종 정유의 치사속도를 조사한 결과, eucalyptus oil과 rosemary oil이 처리 후 6시간 이내에 100%의 살충률을 나타내었다. 각 정유에 대한 주요 구성성분을 GC와 GC/MS로 분석한 결과, eucalyptus oil, rosemary oil, sage oil의 주성분인 1,8-cineole과 sage oil의 또 다른 주성분인 thujone과 pennyroyal의 주성분인 pulegone 모두 20 $\mu\ell/\ell$(공기)의 농도에서 100%의 살충효과를 나타내었다.

• Natural Product Sciences
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• 제14권1호
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• pp.27-31
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• 2008

We have evaluated the combined antifungal effects of the essential oil from Eucalyptus globulus or its main component 1,8-cineole with ketoconazole. Checkerboard microtiter tests were used to analyze their effects against three Candida and six Trichophyton species. The susceptibility of the Trichophyton species to E. globulus essential oil differed distinctly. The fractional inhibitory concentration indices (FICIs) against the tested Candida species ranged between 0.09 and 0.38 for ketoconazole combined with E. globulus essential oil or 1,8-cineole, indicating significant synergism between ketoconazole and the oil samples. Similar experiments using Trichophyton species resulted in FICIs between 0.28 and 0.63, indicating relatively weaker combined effects than those observed with Candida species. Thus, the data reported here show that the anti-Candida effects of ketoconazole can be significantly improved in the presence of E. globulus essential oil or 1, 8-cineole.

• 대한한의학회지
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• 제25권1호
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• pp.40-48
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• 2004

Background and Objectives: Aroma therapy using Eucalyptus Globulus essential oil for curing allergic rhinitis is an inhalation treatment which has been applied as an aid to oriental clinics which use a fundamental treatment such as acupuncture and herbs. There have been no studies so far performed on the effect of this oils' use, only . We aimed to find out the therapeutic effect of its exclusive use only on an Animal Model with Allergic Rhinitis. Materials and Methods: Sprague-Dawley rats were induced the allergic rhinitis by ovalbumin solution. And then, rats in the treated group were treated by diffusing Eucalyptus essential oil in a flask for 7 days. Compared with the treated group, rats in the control group were treated by diffusing normal saline in a flask for 7 days. We observed changes in glandular cells and mast cells in nasal mucosa and submucosa; also changes in the number of eosinophils and neutrophils in blood and the succus of rhinenchysis. Results: The number of neutrophils in the succus of rhinenchysis was significantly decreased in treated group compared with control group(p<0.05). The number of neutrophils in blood and mast cells in nasal mucosa were decreased in the treated group but, these were not significant statistically(p>0.05). Histologic changes showed edema; congestion and expansion of grandular cells in nasal submucosa and hypertrophy of epithelium in nasal mucosa were decreased in treated group compared with control group. Conclusions: The results may suggest that the inhalation treatment using Eucalyptus essential oil decreases the inflammatory response on an Animal Model with Allergic Rhinitis.

• 융합정보논문지
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• 제11권6호
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• pp.198-205
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• 2021

본 배경 : 질염은 항생제를 복용하는 방식으로 치료를 하고 있으며, 이러한 항생제의 지속적인 치료는 내성을 유발할 수 있다. 연구 방법 : Lactic acid bacteria 2종에 에센셜 오일을 이용한 항균 활성을 보고자 한다. SE(Grapefruit Seed Extract), eucalyptus, tea tree, clove bud oil, cinnamon oil, lemongrass oil, thyme oil, ginger oil을 일정 비율로 넣어 배양 후, 병원성 미생물- E. coli, C. albicans와 Lactic acid bacteria은 균주에 맞는 배지를 사용하여 균 수를 측정하였다. 결과 : Essential oil 7종과 GSE가 병원성 미생물에 억제 효과가 있으며, 병원성균(E. coli, C. albicans)에 대한 Grapefruit seed extract(GSE)의 억제농도를 확인하였다, 병원성균은 억제하고 Lactic acid bacteria는 억제하지 않는 배합비도 확인하였다(GSE 농도가 200ppm에서 Eucalyptus globulus(EG) oil 50㎕와 Melaleuca alternifolia oil(Tea tree oil, TTO) 50㎕(pH 5.0, 5.5, 6.0)). 결론 : 본 실험에서 Essential oil은 다양한 항균 활성 가지고 있어 항생제 대안으로도 생각할 수 있으며, 장기 항생제 치료환자에 대한 보조 항균제로서도 유용할 것으로 생각된다.

• 한국미생물·생명공학회지
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• 제48권2호
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• pp.148-157
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• 2020

Eucalyptus oil is a rich source of bioactive compounds with a variety of biological activities and is widely used in traditional medicine. Eucalyptus citriodora is cultivated for the production of essential oils. However, the mode of antibacterial action of essential oils from E. citriodora is not well-known. This study aimed to determine the chemical components, microbial inhibitory effect, and mechanism of action of the essential oil from E. citriodora. The oil was extracted from E. citriodora leaves by hydro-distillation and the chemical components were analyzed using gas chromatography-mass spectrometry. The antibacterial activities of eucalyptus oil against gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus, and Staphylococcus intermedius) and gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) were screened by disc diffusion method and quantitative analysis was conducted by the microdilution method. The mechanism of action of the extracted essential oil was observed using SEM and analyzed by SDS-PAGE. The major components of E. citriodora oil were citronellal (60.55 ± 0.07%), followed by dl-isopulegol (10.57 ± 0.02%) and citronellol (9.04 ± 0.03%). The antibacterial screening indicated that E. citriodora oil exhibited prominent activity against all tested strains. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against B. subtilis were 0.5% and 1.0%, respectively. The MIC and MBC concentrations against S. aureus, S. intermedius, E. coli, and P. aeruginosa were 1% and 2%, respectively. As observed by SEM, the antibacterial mechanism of E. citriodora oil involved cell wall damage; SDS-PAGE revealed decrease in protein bands compared to untreated bacteria. Thus, E. citriodora oil showed significant antimicrobial properties and caused cellular damage.

• Journal of the Korean Wood Science and Technology
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• 제44권4호
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• pp.494-504
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• 2016

Eucalyptus oils are widely used as spices, perfume industrial materials, food flavorings, and medicines. Several types of Eucalyptus oils also have insecticidal activity and as carminative. This study investigated the chemical composition, insecticidal (larvicidal and repellent) activity of E. urophylla oil against filarial mosquito Culex quinquefasciatus. E. urophylla oil was obtained from fresh leaves by water-steam distillation with oil yield 1.08%. E. urophylla oil in this study had no color (clear), has odor (typical eucalyptus), with specific gravity 0.941; refractive index 1.465; miscibility in 70% ethanol 1 : 3; and optical rotation (-) $5.83^{\circ}$. The major compounds of the oil were ${\alpha}$-pinene (11.73%), 1,8-cineole (49.86%), ${\beta}$-ocimene (6.25%), ${\gamma}$-terpinene (9.11%), and ${\alpha}$-terpinyl acetate (7.63%). The result showed the excellent insecticide activity against C. quinquefasciatus. The oil provided larvicidal activity with $LC_{50}$: 80.21 ppm and $LC_{90}$: 210.18 ppm, and repellent activity with $IC_{50}$: 0.82% and $IC_{90}$: 4.88%. The present study showed the effectiveness of E. urophylla as natural insecticide against C. quinquefasciatus, the mosquito vector of filariasis.

• 한국유기농업학회지
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• 제26권2호
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• pp.259-268
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• 2018

The study investigated the efficacy of four different essential oils on the repellent responses of large black chafer (Holotrichia parallela) Motschulsky (Coleoptera: Scarabaeidae) in organic pear (Pyrus pyrifolia) orchards. Cinnamon, pine, peppermint, and eucalyptus oils were used, and the behavioral responses and repellent effects against H. parallela were investigated under laboratory and field conditions. Adult beetle responses to different oils were examined using a Y-tube olfactometer in the laboratory and four absorbent blocks with each oil in the field. The repellent responses rates of H. parallela were 100% for cinnamon oil; however, only 67% of adult beetles avoided peppermint and eucalyptus oil in the Y-tube olfactometer bioassay. In the field tests, the least damage to leaves was observed on trees treated with cinnamon oil, whereas the most damage was observed in the control (non-treated) trees and those treated with peppermint oil, followed by eucalyptus and pine oil. Therefore, cinnamon oil can be used as a repellent to avoid damage form large black chafers in organic pear orchards.

• 한국동물위생학회지
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• 제33권2호
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• pp.167-171
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• 2010

Majority of asthma and atopic dermatitis are known to be spontaneously sensitive to house dust mite allergen. Control of house dust mite populations has been principally achieved by using chemical insecticides. But the risk to human health would be a potential problem. Moreover, house dust mite remain as allergens even after death. So, It is more effective method keeping the house dust mites away than killing them. The use of plant-derived repellents has been considered as a promising alternative to chemical repellents. Eucalyptus is a diverse genus of flowering trees in the myrtle family, Myrtaceae. It has insect repellent properties and is an active ingredient in some commercial mosquito repellents. These studies were carried out to investigate repellent effects of Eucalyptus oil against house dust mites and compare lemongrass oil. Eucalyptus oil and lemongrass oil were exposed at different doses (0.2, 0.1, 0.05, 0.025, 0.0125, $0.00625{\mu}l/cm^2$) and different times (1, 3, 6 hours) on house dust mites. The most effective dose of Eucalyptus and lemongrass oils against house dust mites was $0.1{\mu}l/cm^2$ and $0.025{\mu}l/cm^2$. Each repellent effect(%) in most effective dose was 90.3% (Eucalyptus) and 80.8% (lemongrass).

• The Plant Pathology Journal
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• 제25권2호
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• pp.189-192
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• 2009

The effect of plant extracts of eucalyptus (Eucalyptus chamadulonsis), garlic (Allium sativium), marigold (Tagetes erecta) and neem (Azadirachta indica) and essential oils were tested on the suppression of root-knot nematode Meloidogyne incognita under greenhouse and field conditions. In vitro study, all tested treatments had nematicidal effect on nematode juveniles after 24 and 48 hours from exposures. The highest percentage of nematode mortality was achieved by application of neem extract (65.4%), essential oils (64.4%) and marigold extract (60.5%), followed by garlic and eucalyptus extracts (38.7-39.5%). Under greenhouse and field conditions, neem extract and essential oils treatments were more effective in reducing population numbers of the M. incognita in soil and root gall index compared to other treatments. In field experiments, the maximum protection of tomato plant against root-knot nematode was obtained by application of neem and essential oil treatments, 44.2 and 32.6%, respectively.

• 대한화장품학회지
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• 제37권4호
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• pp.303-308
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• 2011

레몬 오일과 유칼립투스 오일은 노화방지, 살균작용과 같은 다양한 효능을 가진 것으로 알려져 있다. 그러나 특정 피부 상재균에 대한 항균 효과와 항산화 효과는 아직 연구가 미비한 상황이다. 따라서 본 연구에서는 DPPH 라디칼 소거능 실험과 paper disc 방법을 이용하여 단일 오일들과 혼합오일의 항산화와 항균 활성을 검증하고자 하였다. 레몬 오일은 높은 DPPH 소거능을 보였으나 유칼립투스 오일은 아주 약한 소거능을 보였다. 두 오일을 혼합한 경우에는 효과가 증대되지 않았다. Paper disc 기법을 이용한 항균 효과 시험에서는 두 오일 모두 Candida albicans (C. albicans) 와 Propionibacterium acnes (P. acne)에서 항균력이 우수하며 농도의존적인 효능이 있음을 알 수 있었다. 또한, 두 오일 을 혼합 하였을 때 P. acne에 대하여 항균 활성이 상승됨을 알 수 있었다. 본 연구의 결과를 종합적으로 볼 때, 레몬 오일과 유칼립투스 오일의 혼합 오일은 천연성분으로서 균으로 야기된 문제성 피부나 여드름 피부를 개선시키는데 효과적일 것으로 사료된다.