• Journal of Applied Biological Chemistry
• /
• 제60권4호
• /
• pp.293-300
• /
• 2017

와송에 함유된 phenol성 물질은 50% ethanol을 용매로 하여 추출하였을 때 $10.56{\pm}0.32mg/g$의 함량을 나타내었다. 와송 추출물의 항산화력을 측정한 결과 전자공여능은 $200{\mu}g/mL$ phenolic 농도에서 열수 추출물과 50% ethanol 추출물 모두 80.0% 이상으로 높은 효과가 확인되었다. ABTS radical cation decolorization을 측정한 결과에서 와송 열수와 50% ethanol 추출물 모두 95.0% 이상의 높은 항산화 활성이 측정되었다. Antioxidant protection factor (PF)는 열수 추출물이 50% ethanol 추출물에 비해 높은 PF값을 나타내었으며, thiobarbituric acid reactive substance (TBARs) 저해효과는 50% ethanol 추출물이 열수 추출물 보다 높은 항산화력을 나타내었다. 와송 추출물의 항고혈압 효과를 살펴보기 위하여 angiotensin converting enzyme 저해활성을 측정한 결과 $200{\mu}g/mL$ phenolic의 처리농도에서 열수와 50% ethanol 추출물이 각각 6.67%과 7.98%로 낮게 나타났다. 와송 추출물의 XOase 저해활성은 50% ethanol 추출물 $200{\mu}g/mL$ phenolic 농도에서 60.85%의 저해율을 나타내었으며, tyrosinase 억제활성은 50% ethanol 추출물 $200{\mu}g/mL$ phenolic 농도에서 64.59%로 나타나 대조구인 kojic acid 보다 미백 효과가 더 우수함을 입증하였다. 주름 생성과 관련된 collagenase 억제효과는 50% ethanol 추출물의 $50200{\mu}g/mL$ phenolic 농도에서 75.9585.02%로 매우 우수하였으며, 대조구인 epigallocatechin-gallate의 68.9176.64% 보다 다소 높았다. Elastase 저해활성도 50% ethanol 추출물 $200{\mu}g/mL$ phenolic 농도에서 최대 63.65%의 저해효과를 나타내어 와송 50% ethanol 추출물의 주름개선 효과가 매우 우수함을 확인하였다. 이상의 결과를 통해 와송 추출물의 항산화 활성, 통풍 억제효능, 미백 및 주름개선 등의 기능성 검증을 위한 기초 연구 자료로 활용이 가능할 것으로 예상되며, 향후 추가적인 연구를 통해 와송 추출물을 기능성 식품 및 화장품, 의약품 소재로 적용이 가능할 것으로 판단되었다.

• 한국산학기술학회논문지
• /
• 제18권3호
• /
• pp.513-517
• /
• 2017

본 연구는 향장품 소재로 물이 IC50 680.98ppm으로 가장 높은 활성을 나타내었다. ABTS를 이용한 활성 산소 소거능에서는 50% 에탄올 추출물과 70% 에탄올 추출물에서 각각 IC50 646.94, IC50 661.94ppm으로 높은 활성을 보였다. 각 에탄올 추출물은 일정농도(100-10000ppm)에서 항산화 활성을 보였으나 에탄올 추출 농도와는 유의적인 관계를 보이지 않았다. LPS로 염증이 유도된 RAW 264.7 macrophage에 각각의 에탄올 추출물을 처리한 경우 모든 추출물에서 NO생성을 감소시키는 것을 확인 할 수 있었다. 50% 이상의 에탄올 추출물(10000ppm)에서 85%이상의 nitric oxide 생성 억제율을 보였으며, 특히 70% 에탄올 추출물에서 90% 이상의 nitric oxide 생성 억제율을 보였다. 또한 MTT assay를 실시한 결과 각 추출물의 모든 농도에서(1250-10000ppm) 세포독성이 없음을 확인 할 수 있었다. 본 연구를 통해 자소엽 에탄올 추출물은 세포독성이 없는 천연물 유래의 소재로서 각 추출농도에서 농도 의존적으로 항산화 활성과 항염활성을 가짐을 확인할 수 있었다.

• Journal of Nutrition and Health
• /
• 제37권10호
• /
• pp.872-880
• /
• 2004

This study was performed to investigate the effect of Puerariae radix-ethanol extracts rich in isoflavone on the antio-xidative system of rats. For this purpose, first, Puerariae radix was extracted with ethanol, and its total isoflavone and puerarin contents were analysed. Second, female Sprague Dawley rats were fed for 6 weeks with four diets which were based on AIN96G diet and supplemented with Puerariae radix-ethanol extracts to contain isoflavone. The isoflavone contents of four experimental diets were 0 mg, 500 mg, 1,000 mg, 2,000 mg per kg diet, respectively (control, P0.05%,P0.1%, P0.2%). Liver and erythrocyte activities of antioxidative enzyme such as superoxide dismutase (SOD), catalase,glutathione peroxidase (GSHpx) were measured. Also, plasma and liver malondialdehyde (MDA) concentrations, liver glutathione (GSH) and oxidized glutathione (GSSG) concentrations were measured. The total isoflavone content of Puerariae radix-ethanol extract was 3067.6 mg per 100 g extract and the content of puerarin was 2557.4 mg per 100 g extract. The erythrocyte activities of GSH-Px and catalase were higher in group P0.1% and P0.2%. But SOD activity of erythocyte did not show any difference by the Puerariae radix-ethanol extract supplementation in diet. The activity of SOD in liver increased significantly by the supplementation of extract, showing highest level in P0.1% group. The liver GSH concentration increased significantly in group of P0.05%, P0.1%, and P0.2% compared with control group (p <0.05). The GSSG concentration in liver showed no difference by the supplementation of Puerariae radix extract from the control group, except P0.2% group. The plasma MDA concentration did not show any significant differences by the extract supplementation. But the liver MDA concentration decreased by the extract supplementation, showing the lowest level in P0.1 % diet group. These results suggest that the supplementation of Puerariae radix-ethanol extract can inhibit lipid peroxidation in liver and enhance the antioxidative defense competence of rats.

• 동아시아식생활학회지
• /
• 제16권2호
• /
• pp.199-206
• /
• 2006

This study was designed to investigate the effect of medicinal herb extract on the antioxidant and antimicrobial activities against Helicobacter pylori, which is known as a ulcerogenic pathogen. The concentration of total phenolic compound of Scutellaria baicalensis(13.14%) was highest among water extracts and that of Ulmus parvifolia(15.12%) was highest among the ethanol extracts. The antioxidant activity of the water extract of Scutellaria baicalensis and of the ethanol extract of Ulmus parvifolia were 91.00% and 65.03%, respectively, in DPPH assay. The antioxidant activity of the water extract of Scutellaria baicalensis and of the ethanol extract of Ulmus parvifolia were 32.90% and 27.70%, respectively, in SOD assay. The antioxidant activity of the water extract of Ulmus parvifolia and of the ethanol extract of Glycyrrhiza uralensis Fischer was 2.15 and 2.17, respectively, in TBARS assay. In disc method, Scutellaria baicalensis showed the highest anti-microbial activity against H. pylori, followed by Glycyrrhiza uralensis Fischer among the water extracts and Glycyrrhiza uralensis Fischer showed the highest anti-microbial activity followed by Radix puerariae among ethanol extract.

• 환경위생공학
• /
• 제23권2호
• /
• pp.65-70
• /
• 2008

The purposes of this study were to investigate the effects of bamboo (SasacoreanaNakai)extracton chemical characteristics and biological activities. pH ranges of bamboo extract using water and ethanol were between 5.2 and 6.1. The concentration of total phenol compounds using ethanol extract was 0.51%, which was about 2.0 fold higher than that of water extract. The mineral concentrations were order of K, Mg, Ca, and Na, respectively. The nitrite scavenging ratio of bamboo extract using ethanol was 20.4% in vitro. In the case of bamboo extract using water, it was 18.0%. Especially, the maximum nitrite scavenging ratio was obtained at pH 1.5. On the other hand, when pH was increased from 3.0 to 6.0, the nitrite scavenging ratio was decreased from 16.2% to 3.0%. The antioxidant activity of bamboo extract in vitro was increased from 100 to 160% when bamboo extract using ethanol was increased from 10 to $50{\mu}L$. These results suggest that the bamboo extract of Sasa coreana Nakai using ethanol can be used in bioactive and functional material.

• 대한본초학회지
• /
• 제34권6호
• /
• pp.109-115
• /
• 2019

Objective : Herbal medicinal mixture (JMB) are consisted of Caryophylli Flos, Aucklandiae Radix, and Angelicae Dahuricae Radix. Each of these herbal medicines has studied on anti-aging effect in vitro. So this study was conducted to investigate efficacy and potency of JMB extract on dermal anti-aging and whitening. Methods : The JMB was extracted at room temperature by 80% ethanol. Collagenase and elastase inhibition activity in JMB ethanol extract were determined at 10, 50, 100, 500, 1000 mg/ml concentrations by colorimetric method. The toxic range of JMB ethanol extract was evaluated using MTT assay. Also, The inhibitory effect of JMB ethanol extract on tyrosinase activity and melanin contents in mouse melanoma cell line (B16F10 cell) was identified at 50, 100, 200 ㎍/㎖ levels by spectrometric assay. In each analysis, EGCG (epigallocatechin gallate) and Kojic acid were used as positive controls, respectively. Results : The elastase and collagenase inhibitory activity of JMB ethanol extract increased dose dependently. Also, The MTT assay showed that JMB, up to 400 ㎍/㎖ concentration, exhibited no toxic effect to the B16F10 cell. And following the JMB ethanol extract treat, cellular melanin contents and tyrosinase activity were dose-dependently decreased compared to those of control. Conclusion : These results suggest that JMB ethanol extract has effects to inhibitory activity on dermal wrinkle enzyme and melanogenesis. Therefore, JMB has applicable benefits for development of materials or products to have whitening and anti-aging functions on skin.

• Journal of the Korean Wood Science and Technology
• /
• 제50권5호
• /
• pp.338-352
• /
• 2022

Herein, water and ethanol extracts were obtained from the leaves, branches, kernels, and pericarp of Quercus gilva and subsequently analyzed for antioxidant activity and circadian rhythm regulation effects. Candidate components that may affect circadian rhythm and antioxidant activity were investigated to discover potential functional materials. Antioxidant activity was analyzed via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity assays, showing that the hot water extract exhibited higher activity than that of the ethanol extract. In particular, the branch extract showed high antioxidant activity. By measuring total contents of polyphenols, flavonoids, and tannins, the hot water branch extract showed the highest concentrations, highlighting their significant contribution to the antioxidant activity. Examination of the circadian rhythm regulation of each extract showed that the ethanol extract exhibited greater impacts on the circadian rhythm amplitude compared to the water extract. The branch ethanol extract induced circadian rhythm amplitude changes via clock gene Bmal1 expression regulation. Determination of 12 phenolic compound concentrations showed that the branch ethanol extract contained many phenolic compounds, including catechin. This suggests that these com- pounds affected circadian rhythm regulation. In conclusion, the hot water branch extract has potential as an natural antioxidant material, while the corresponding ethanol extract has potential as a functional material for regulating circadian rhythm.

• 한국조리학회지
• /
• 제22권6호
• /
• pp.14-23
• /
• 2016

This purpose of this study is to examine the antioxidant and antimicrobial activities of Persicaria hydropiper L. extract in 70% ethanol and in water, a medicinal herb, as an effort to examine the potential of medicinal herbs for development of antioxidants and natural preservative substitutes. The total poly-phenol content in the 70% ethanol extract and in the water extract were 19.88 mg/g and 7.46 mg/g, respectively. The DPPH radical scavenging activity was 90.99% and 64.98% in the 70% ethanol extract and water extract, in which 70% ethanol extract showed a higher activity. The antioxidant effect of Persicaria hydropiper L. extract appears to be very good and due to its excellent growth inhibitory effect on food-poisoning-causing microorganisms in the food, it is thought to be utilized as a potential natural preservative substitute in many areas.

• 동의생리병리학회지
• /
• 제27권4호
• /
• pp.391-399
• /
• 2013

The aim of the present study was to investigate the antioxidant efficacies between extracts of Prunus yedoensis(PY) and Betulae platyphyllae var. japonica(BP). HPLC pattern was different between barks and extract solvents. Content of total phenolic compound was the highest in ethanol extract of BP(382.201 mg/g ext.) and its content was 1.9 times higher than that from the water extract of PY. Total antioxidant efficacy also was the highest in ethanol extract of BP (292 copper reducing equivalents). Nitric oxide scavenging activity was almost 70% in ethanol extract of BP treated 200 ug/ml and it was higher than positive control(ascorbic acid). DPPH radical scavenging ability was up by 80% in all samples. ABTS cation decolorization from each barks was activated over 85% in all samples at 100 ug/ml concentration, especially, the activity was the highest (94.4%) in ethanol extract of BP. Hydrogen peroxide scavenging activities were also highest (45%) in ethanol extract of BP at 200 ug/ml concentration and were as high as positive control. Stimulation of the macrophages RAW 264.7 cells with lipopolysaccharide (LPS) increased intracellular ROS levels and ethanol extract of BP at 200 ug/ml concentration reduced ROS levels up to 41 %. The results indicated that the barks of PY and BP has potent antioxidant activities and ethanol extract of BP of them has the highest antioxidant activities.

• 대한본초학회지
• /
• 제37권6호
• /
• pp.37-44
• /
• 2022

Objectives : From this study, we sight to identify chondro-protective and anti-inflammatory effects of Sorbi Commixtae Fructus extract and its compound, chlorogenic acid. Methods : Sorbi Commixtae Fructus were extracted by 50% ethanol. And chlorogenic acid in Sorbi Commixtae Fructus 50% extract was quantified by high performance liquid chromatography (HPLC). To investigate chondro-protective effects, we treated Sorbi Commixtae Fructus 50% ethanol extract and chlorogenic acid in TNF𝛼-activated ATDC5 murine chondrogenic cells. After 24 hours, protein level of matrix metalloproteinase-3 (MMP3) and mRNA level of matrix metalloproteinase-13 (MMP13) were measured by using ELISA or reverse transcription PCR, respectively. To examine anti-inflammatory effects, we treated Sorbi Commixtae Fructus 50% ethanol extract and chlorogenic acid in LPS-induced RAW 264.7 murine macrophages. We measured the level of inflammatory mediators, such as Prostaglandin E2 (PGE2), Interleukin-6 (IL6) by ELISA and nitric oxide (NO) by Griess reagent assay. Results : A concentration of chlorogenic acid in Sorbi Commixtae Fructus 50% ethanol extract was 3.9 mg/g. Sorbi Commixtae Fructus 50% ethanol extract and chlorogenic acid attenuated protein level of MMP3 and mRNA level of MMP13 in TNF𝛼-activated ATDC5 cells. Sorbi commixtae Fructus 50% ethanol extract inhibited the level of PGE2, IL6 and NO in LPS-activated RAW 264.7 cells in dose dependent manner, but chlorogenic acid has no anti-inflammatory effects. Conclusions : These findings demonstrated that Sorbi Commixtae Fructus 50% ethanol extract has chondro-protective and anti-inflammatory effects showing possible therapeutics to ease the symptoms related with osteoarthritis.