• 생약학회지
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• 제38권4호
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• pp.358-362
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• 2007

From the EtOAc fraction of the MeOH extract of Ulmus davidiana, (-)-catechin (1), (-)-catechin-7-O-${\beta}$-D-apiofuranoside (2), and (-)-catechin-7-O-${\beta}$-D-xylopyranoside (3) were isolated and characterized on the basis of $^1H-and\;^{13}C-NMR$, and FABMS spectral data. Compounds 1-3 showed more strong reducing power activities than ${\alpha}-tocopherol$, a positive control.

• 생약학회지
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• 제45권1호
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• pp.35-40
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• 2014

In this study, in order to search for new functional materials from natural products, was carried out the study of antioxidant active ingredients in persimmon calyx(calyx of Diospyros kaki Thunberg). I have experimented with the effect of antioxidant activity of five different extract(MeOH, n-hexane, EtOAc, n-BuOH and $H_2O$ extract) obtained from persimmon calyx. As a result, the butanol extract, that is the main component fraction of antioxidant activity was found. Three compounds were isolated by silica gel column chromatography from the n-BuOH extract of persimmon calyx. Their structures of compound 1, 2 and 3 isolated from n-BuOH extract of persimmon calyx were identified as quercetin, (+)-catechin and gallic acid by using the TLC, $^1H$-NMR and $^{13}C$-NMR.

• Journal of Applied Biological Chemistry
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• 제48권1호
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• pp.32-34
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• 2005

Arils of Euporia longana L. was extracted with 80% aqueous MeOH and partitioned successively with EtOAc, n-BuOH and $H_2O$. From the n-BuOH fraction, furan compound was isolated through silica gel column chromatography. The results of physico-chemical data including NMR, MS and IR revealed the compound to be 5-(hydroxymethyl)-2-furfuraldehyde. This compound stimulated GDH I activity by $19.2{\pm}0.6$, $41.2{\pm}0.9$, $68.4{\pm}1.1$, $80.3{\pm}0.9$ and $85.9{\pm}1.6%$ at in vitro concentrations of 0.005, 0.008, 0.02 and 0.03 %, respectively.

• Journal of Applied Biological Chemistry
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• 제49권4호
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• pp.140-142
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• 2006

The aim of this study is to investigate in vitro biological properties of Eucommia ulmoides Oliv. bark. Ethyl acetate(EtOAc) fraction from aqueous extract of Eucommia bark showed strong antioxidant activity of $IC_{50}$ 19.2 ${\mu}g/ml$ by 1,1-diphenyl-2-picrylhydrazyl(DPPH) free radical-scavenging assay. The Eucommia bark extract showed $\alpha$-glucosidase inhibitory activity and inhibited growth of human liver cancer cell, suggesting its potential biological value of anticancer.

• 한국자원식물학회지
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• 제29권6호
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• pp.690-698
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• 2016

Various bioactive substances are found in mistletoe, including viscumneoside III (1) and homoflavoyadorinin B (2), both which inhibit tyrosinase. These two compounds are mainly found in the EtOAc fraction of the mistletoe extract and demonstrate higher rates of tyrosinase inhibition than ascorbic acid, which was used as a control. Our results suggest that mistletoe extracts can be utilized in skin whitening cosmetics.

• 한국약용작물학회지
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• 제3권2호
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• pp.91-95
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• 1995

국내(國內)에서 생산(生産)된 백화사설초(百花蛇舌草) Oldenlandia diffusa(WiIId.) Roxb. 의 전초로 부터 얻은 MeOH extract를 $CHCl_3, EtOAc$ 및 BuOH로 분획하여 얻은 fraction으로 부터 column chromatography를 실시하여 3종의 화합물을 분리하였다. 이들의 이화학적(理化學的) 및 분광학적(分光學的) data를 종합하여 화합물 I의 화학구조는 ${\beta}-sitosterol$에 소양(小量)의 stigmasterol이 함유된 sterol이며, 화합물 II는 ursolic acid이고, 화합물 III은 ${\beta}-sitostero\;3-0-{\beta}-D-glucoside$임을 밝혔다. 이 식물은 다양한 생리활성(生理活性)이 보고(報告)된 ursolic acid의 중요한 자원식물(資源植物)이 될 수 있음을 본실험을 통하여 밝혔으며 문헌(文獻)에 보고(報告)된 oleanolic acid는 함유(含有)되어 있지 않음을 알았다.

• 한국자원식물학회지
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• 제30권6호
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• pp.618-622
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• 2017

Various bioactive substances are found in Loranthus tanakae, including quercetin 3-rhamnoside (1), kaempferol 3-rhamnoside (2), rhamnetin 3-rhamnoside (3), and rhamnocitrin 3-rhamnoside (4), which inhibit tyrosinase. These compounds are mainly found in the EtOAc fraction of L. tanakae extract and demonstrate higher rates of tyrosinase inhibition than ascorbic acid, which was used as a control. Our results suggest that L. tanakae extracts can be utilized in skin whitening cosmetics.

• Archives of Pharmacal Research
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• 제29권3호
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• pp.191-194
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• 2006

Twigs from llex macropoda were extracted with MeOH, and the concentrated extracts were partitioned with $CH_2Cl_2$, EtOAc, n-BuOH, and $H_2O$. Repeated column chromatography of the $CH_2Cl_2$ fraction ultimately resulted in the isolation of two compounds, via activity-guided fractionation, using ACAT inhibitory activity measurements. According to the physico-chemical data, the chemical structures of these isolated compounds were identified as lupeol (1) and betulin (2). Compounds 1 and 2 were shown to inhibit the activity of hACAT-1 and hACAT-2 in a dose-dependent manner, and compounds 1 and 2 inhibited hACAT-1 with $IC_{50}$ values of 48 and $83{\mu}M$, respectively.

• 생약학회지
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• 제43권3호
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• pp.206-212
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• 2012

Ten flavonol glycosides were isolated from the EtOAc fraction of the MeOH extract of Metaplexis japonica Makino. Structures of the flavonoids were elucidated on the basis of spectroscopic data and comparison with literature values. The flavonoids were found to be mostly common flavonol 3-glycosides. It is of interest that the sacchaide parts of the isolates were pairs of arabinosides, glucosides, galactosides, rutinosides and robinobiosides of kaempferol and quercetin. All of these compounds were isolated for the first time from this plant.

• Natural Product Sciences
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• 제12권4호
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• pp.197-200
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• 2006

Two phenolic acids, gallic acid (1) and syringic acid (2), and five flavonoids, apigenin (3), luteolin (4), kaempferol (5), quercetin (6), and myricetin (7), were isolated from the aerial parts of Leonurus japonicus. Their structures were elucidated by chemical and spectral analysis. The antioxidant activities of the crude extracts, partitioned fractions and isolated compounds were evaluated by DDPH free radical-scavenging assay. Results suggested that the EtOAc partitioned fraction and compounds 1, 4, 5, 6, and 7 showed significantly high antioxidant potential compared with $\alpha-tocopherol$ and BHT, which were used as controls.