• 한국식품위생안전성학회지
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• 제28권3호
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• pp.234-240
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• 2013

본 연구는 E. coli와 S. typhimurium을 대상으로 orthophenylphenol 20%를 함유한 훈증소독제, Fumagari OPP$^{(R)}$의 살균효과를 평가하기 위해 수행되었다. 예비 시험에서, E. coli와 S. typhimurium의 현탁액 균수는 모두 $4.0{\times}10^8CFU/mL$이었으며, 모든 훈증소독제에 노출시킨 담체의 균수는 모두 평판배지법과 여과법으로 배양한 시험균주 현탁액의 균수의 50%보다 많았다. 또한, 대조 담체로부터 회복된 E. coli와 S. typhimurium 균수는 모두 $3.4{\times}10^6CFU/mL$이었다. 훈증소독제의 살균효과 시험에서는, 훈증소독제를 처리한 담체의 E. coli와 S. typhimurium의 감소 균수는 각각 5.64와 5.26 logCFU/mL로 나타났다. 이상의 결과로부터, 훈증소독제, Fumagari OPP$^{(R)}$은 E. coli와 S. typhimurium에 대해 효과적인 살균력을 갖는 것으로 확인되었으며, 병원성 세균에 오염된 식품재료 및 주방용품의 소독에 적용할 수 있을 것으로 사료된다.

• Childhood Kidney Diseases
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• 제6권1호
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• pp.102-108
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• 2002

저자들은 stx2 유전자 양성인 E.coli O114 에 의한 용혈성 요독 증후군 2례를 경험하여 보고하는 바이다. 두 환자 모두 설사와 구토가 1-2일 지속된 후 급작스럽게 요량 감소와 빈혈, 혈소판감소증을 보였으며 10일 간의 복막 투석 치료를 받았으며 급성기에 혈압 증가와 함께 전신성 강직 간대성의 경련이 15-20분 간 있었다. 15여 일 경과 후 후유증 없이 회복되었다. 대변 배양액의 PCR에거 eae와 stx2 유전자를 확인하였고 colony hybridization을 시행하여 O114 혈청형으로 진단하였다. 본 증례는 E.coli O114에 의해 유발된 용혈성 요독 증후군의 첫 국내 보고이다.

• Journal of Microbiology and Biotechnology
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• 제21권10호
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• pp.1053-1056
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• 2011

Optimal conditions for a high cell density fermentation were investigated in a recombinant Escherichia coli producing salmosin, a platelet aggregation inhibitor. The optimized carbon and nitrogen sources were glycerol 10 g/l, yeast extract 30 g/l, and bacto-tryptone 10 g/l, yielding the dry cell weight (DCW) of 10.61 g/l in a 500 ml flask culture. The late-stage induction with 1% L-arabinose in a 5 l jar fermentor showed the highest DCW of 65.70 g/l after 27 h of the fed-batch fermentation. Around 2,200 mg/l of the protein was expressed as an inclusion body that was then refolded to obtain the active salmosin of 96 mg/l. We also confirmed the inhibitory activity against platelet aggregation of the active salmosin from the high cell density fermentation.

• 대한의생명과학회지
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• 제17권3호
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• pp.173-176
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• 2011

Free-living Acanthamoeba are eukaryotic protozoan organisms that are widely distributed in the air, water, etc such as environment. Acanthamoeba ingest the Escherichia coli which will replicate in cytoplasm of Acanthamoeba. Bacterial pathogenicity or virulence is one of important determinant factors to survive in free-living Acanthamoeba and otherwise Acanthamoebic pathogenicity is also an important factor for their interactions. Bacterial association with pathogenic strain of Acanthamoeba T1 and T4 was lower about two times than non-pathogenic T7. Bacterial invasion percentages into T1 were higher about three times than T7 but bacterial survival in T7 was increased as T1. The capsule-deletion mutant exhibited limited ability for invasion/uptake by and survival inside pathogenic Acanthamoeba T4. E. coli-outer membrane protein A (OmpA) decreased bacterial association with A. castellanii by about three times and it had higher effects than lipopolysaccharides (LPS). Under favorable conditions, the mutants were not survived in Acanthamoeba up to 24 h incubation. Therefore, this review will report pathogenic and non-pathogenic Acanthamoeba strains interactions with E. coli and its several mutants, i.e., capsule, OmpA and LPS.

• Food Science and Biotechnology
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• 제16권2호
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• pp.294-298
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• 2007

The actinidin (EC 3.4.22. 14) found in kiwifruit is a cysteine protease. In order to obtain the actinidin gene from the Chinese wild kiwifruit, primers were designed on the basis of the actinidin gene of Actinidia deliciosa, the New Zealand kiwifruit. The 1.2 kb DNA fragment was acquired from the total RNAs of Chinese wild kiwifruit via reverse transcription polymerase chain reaction (RT-PCR), and its DNA sequence was analyzed. Its sequence was determined to share 98.4% homology with the actinidin gene of A. deliciosa. In order to verity the actinidin gene isolated from the Chinese wild kiwifruit in Escherichia coli, the mature gene was amplified via PCR and expressed in E. coli under the control of the T7lac promoter. The actinidin was expressed in E. coli as inclusion bodies, which were solubilized with urea and refolded. The protease activity of the refolded protein was approximately twice as high as that of E. coli BL2l (DE3).

• 대한의생명과학회지
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• 제13권3호
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• pp.169-173
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• 2007

[ $^{31}PNMR$ ] spectroscopy revealed the adenylate kinase-like activity and the phosphotransferase activity from $F_1-ATPase$ of Escherichia coli. Incubation of $F_1-ATPase$ with ADP in the presence of $Mg^{2+}$ shows the appearance of $^{31}P$ resonances from AMP and Pi, suggesting the generation of AMP and ATP by adenylate kinase-like activity and the subsequent hydrolysis to Pi. Incubation of $F_1-ATPase$ with ADP in the presence of methanol shows additional peak from methyl phosphate, suggesting phosphotransferase activity of $F_1-ATPase$. Both adenylate kinase-like activity and the phosphotransferase activity has not been reported from $F_1-ATPase$ from Escherichia coli. $^{31}P$ NMR proved that it could be a valuable tool for the investigation of phosphorous related enzyme.

• Environmental Engineering Research
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• 제14권1호
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• pp.63-67
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• 2009

Escherichia coli K-12 (E. coli K-12) is a representative indicator globally used for distinguishing and monitoring dynamic fates of pathogenic microorganisms in the environment. This study investigated how to most critically quantify lacZ ($\beta$-galactosidase) gene in E. coli K-12 by two different real-time polymerase chain reaction (real-time PCR) in association with three different DNA extraction practices. Three DNA extractions, i.e., sodium dodecyl sulfate (SDS)/proteinase K, magnetic beads and guanidium thiocyanate (GTC)/silica matrix were each compared for extracting total genomic DNA from E. coli K-12. Among them, GTC/silica matrix and magnetic beads beating similarly worked out to have the highest (22-23 ng/${\mu}L$) concentration of DNA extracted, but employing SDS/proteinase K had the lowest (10 ng/${\mu}L$) concentration of DNA retrieved. There were no significant differences in the quantification of the copy numbers of lacZ gene between SYBR Green I qPCR and QProbe-qPCR. However, SYBR Green I qPCR obtained somewhat higher copy number as $1{\times}10^8$ copies. It was decided that GTC/silica matrix extraction or magnetic beads beating in combination with SYBR Green I qPCR can be preferably applied for more effectively quantifying specific gene from a pure culture of microorganism.

• 미생물학회지
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• 제42권4호
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• pp.252-256
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• 2006

본 연구에서는 지역사회 일반인에게 분리된 대장균과 종합병원을 내원한 설사환자에서 분리한 대장균에 대하여 항생제 내성현황을 모니터링 하였다. 2006년 2월$\sim$7월까지 인천의 지역사회 일반인 692명의 대변으로부터 순수하게 분리된 대장균 491 균주에 대해 항생제 내성균을 조사한 결과, tetracycline 내성이 46.6%, ampicillin 내성이 41.1% ticarcillin 내성이 37.9%였다. 또한 3차 의료기관의 설사환자에서 분리된 대장균 120 균주에 대하여 ampicillin 내성이 66.9%, ticareillin 내성이 63.8%, tetracycline 내성이 47.2%였다. $\beta$-lactam 분해효소(extended spectrum $\beta$-lactamase)를 생성하는 대장균은 지역사회 일반인의 경우 0.6%, 설사질환자의 경우4..1%로 각각 분리되었다.

• 한국동물위생학회지
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• 제19권2호
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• pp.139-153
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• 1996

Porcine E coli infection is a disease caused by Enterotoxin produced by Enterotoxigenic Escherichia coli(ETEC). Enteric colibacillosis has become an economically important disease in pigs as a result of increasing intensification of farrowing management. The present study undertaken to obtain the antibiotic sensitivity and distribution of serogroups and pili producibility test of ETEC from E. coli isolates in Chonnam. The results obtained were as follows. 1. A total of 71 isolates identified as E, coli employing IMViC system from rectal specimens of 54 piglets with diarrhea. 2. In antibiotic sensitivity test, isolates showed high sensitivity to AN, CM, Fox, GM, but resistance to EM, NA TC. 3. The distribution of 25 Isolates of serogroups were 0141:K85(11.3%), 08:K87(8.5%), 064:K (5.6%), 0138:K8l (4.2%), 0139 :K82(2.8%), 0157:K88ac(1.4%) and 0149:K9l (1.4%). 4. MRHA of guinea pig erythrocytes was detected in 8 out of 25OK serotypes and 9 out of 46 unidentified serotypes. MRHA titers of serotypes showed from 64 to 128 in 0141: K85, 2 in 0138:K8l and no titers in 0139:K82. 5. The production of heat labile enterotoxin of ETEC was detect 39 out of 52 isolates showed $\beta$-hemolysin, 7 out of 52 isolates showed ${\gamma}$-hemolysin and 6 out of 52 isolates showed ${\gamma}$-hemolysin by $GM_1$ganglioside ELISA. The distribution of LT toxin were in 12 isolate showed $\beta$-hemolysin, 2 isolates showed ${\alpha}$-hemolysin and 3 isolates showed ${\gamma}$-hemolysin in 25 OK serotypes.

• Journal of Microbiology
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• 제35권2호
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• pp.123-126
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• 1997

The aim of the present study was to optimize culture condition for the expression of lipocortin-1$_{1-185}$ in a recombinant of Escherichia coli using batch system. Plasmid (pHT22) carrying lipocortin-1$_{1-185}$ gene was well maintained in the recombinant with the addition of amplicillin as a selection pressures. Optimum temperature was 28$^{\circ}C$ for seed culture and 4$0^{\circ}C$ for main culture and the optimum pH was 7.0. The production of Lipocortin-1$_{1-185}$ was closely associated with cell growth and related to plasmid amplification.