• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.226.2-226.2
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• 2003

The FimH subunit of type 1-fimbriated Escherichia coli has been determined as a major cause of urinary tract infection. To produce a possible vaccine antigen against urinary tract infection, the fimH gene was genetically linked to the Itxa2b gene, which was then cloned into the pMAL -p2E expression vector. The chimaeric construction of pMALfimH/Itxa2b was transformed into Escherichia coli TB1 and its N-terminal amino acid sequence was analyzed. (omitted)

• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2003년도 추계학술대회초록집
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• pp.30-30
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• 2003

Escherichia coli strains associated with diarrhea have been divided into the following six major categories on the basis of pathogenic mechanisms: enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), enteropathogenic E. coli (EPEC), enterohemorrhagic E. coli (EHEC), enteroaggregative E. coli (EAggEC) and diffusely adherent E. coli (DAEC).$\^$15,18/ EPEC, EAggEC, and DAEC strains were classified by their ability to produce distinct patterns of adherence to cultured epithelial cells in virto: localized (LA), aggregative (AA), and diffuse (DA) adherence. The objective of this study was to investigate the relationship of the adherence patterns with AIDA-positive E. coli isolated from diarrheic pigs. (omitted)

• 미생물학회지
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• 제23권1호
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• pp.49-55
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• 1985

The survival of Escherichia coli in river water was comparatively studied by laboratory and in sity study methods. The survival by two methods was evaluated as a function of E. coli strain, indigeneous predator, level of water pollution, and water temperature in different season. The survival rate of E. coli examined by laboratory method was lower than that by in situ method. That was found to be due to the fact that higher number of predator was maintained in labortory study than in in situ study. The survival rates of E. coli in gradually polluted river waters could be differentiated by in situ study, but not by laboratory study. Therefore, an in situ method rather than labortory method was thought to be a choice of study method for the survival of E. coli in river waters.

• Journal of Microbiology and Biotechnology
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• 제9권6호
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• pp.722-725
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• 1999

Filamentation-suppressed recombinant Escherichia coli strain harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes and the E. coli ftsZ gene was constructed and cultivated for the production of poly(3-hydroxybutyrate) [P(3HB)] with high concentration and high content. By the pH-stat fed-batch culture of this recombinant E. coli strain XL1-Blue(pJC5), the final cell concentration and P(3HB) concentration obtained in 44.25h were 172.2g cell dry weight/l and 141.9g P(3HB)/l, respectively, resulting in productivity of 3.21g P(3HB)/l-h. More importantly, the P(3HB) content obtained was 82.4 wt %, which was significantly higher than that obtained with the recombinant E. coli harboring only the PHA biosynthesis genes.

• 미생물학회지
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• 제27권2호
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• pp.147-154
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• 1989

Vibrio vulnificus Lipopolysaccharide (LPS) was extracted, performed chemical analysis, tested its biological activities, and compared to those of Escherichia coli LPS and Salmonella typhimurium LPS. The lethal activity of V. vulnificus LPS was 138.6138.6 mg/kg in mouse, but this was lower than thowe of E. coli LPS (56.3 mg/kg) and S. typhimurium LPS (37.5 mg/kg). The result of fatty acid analysis showed that V. vulnificus LPS had more saturated fatty acid than E. coli LPS and S. typhimurium LPS. Above results indicated that V. vulnificus LPS did not have much effect on the lethality. The results of biological responses of enzymes and blood cells by LPSs showed that V. vulnificus LPS had slightly greater activity than E. coli LPS and S. typhimurium LPS. V. vulnificus LPS was recommendavle for stimulant on interferon induction because of adequate stimulation and safety for host and cell lines.

• Journal of Microbiology and Biotechnology
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• 제16권9호
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• pp.1448-1452
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• 2006

The effects of amplifying the gluconeogenic phosphoenolpyruvate carboxykinase of Escherichia coli ($pck_{Ec}$) on succinic acid production in E. coli were examined under anaerobic condition. No significant increase in succinic acid production was observed in E. coli overexpressing the $pck_{Ec}$ gene without supplementing $NaHCO_{3}$ or $MgCO_{3}$. On the other hand, succinic acid production was enhanced as the $NaHCO_{3}$ concentration was increased. When 20 g/l of $NaHCO_{3}$ was added, succinic acid production in recombinant E. coli overexpressing PCK was 2.2-fold higher than that observed in the wild-type strain. It was concluded that the gluconeogenic $pck_{Ec}$ overexpression enabled E. coli to enhance succinic acid production only under the high bicarbonate supplementation condition.

• Journal of Microbiology and Biotechnology
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• 제21권3호
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• pp.252-255
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• 2011

Both Escherichia coli (E. coli) and Salmonella enterica serovar Typhimurium (S. Typhimurium) have a tdc operon that encodes enzymes involved in a metabolic pathway for the degradation of L-serine and L-threonine. However, S. Typhimurium does not have the tdcR gene, which is a positive regulator in E. coli. In the present study, transcriptional analysis revealed that tdcA expression in E. coli is higher under anaerobic than aerobic growth conditions, but the opposite is true in S. Typhimurium. Interestingly, a tdcR mutant strain of E. coli showed a similar expression pattern to that observed in S. Typhimurium and was also induced by anaerobic shock. These results suggest that the induction of tdcA expression by anaerobic conditions is observable when tdcA expression is low owing to the absence of TdcR.

• 한국식품위생안전성학회지
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• 제22권2호
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• pp.116-119
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• 2007

돼지로부터 분리한 eae+Escherichia coli 67주에 대한 항생제 감수성 시험 결과, Ne에 41.8%, Li에 74.6%, DFX에 73.1%, ENR에 64.2%, Cef에 98.5%의 감수성을 나타내었다. 총 8종의 항생제에 대한 E. coli의 내성패턴을 분석하였을 때 12가지 내성 패턴을 나타내었으며, 그 중 4 제, 3 제 및 6제에 각각 26주(39%), 16 주(24%), 10주(14.9%)로 높았으며, 7종 항생제에 대해 내성을 나타내는 균주도 6주(8.9%)가 확인되었다. 본 실험에 의하면 최근에 사용되기 시작한 항생제의 경우 항생제 내성의 출현이 활발하지 않았으며, 지속적으로 노출된 항생제에 대해서는 감수성이 현저히 낮은 것으로 나타났다. Penicillin, Tetracycline, Neomycin은 본 실험에서 100%의 내성을 나타내며 돈육에서 분리되는 대장균간에 내성 전이가 활발한 것으로 추정되었다.

• 대한수의학회지
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• 제43권4호
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• pp.625-631
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• 2003

The extracts from 40 different traditional medicinal herbs were used to investigate the antimicrobial activities against Escherichia coli. Among them, the extracts from Paeonia suffruticosa (PS), Siegesbeckia orientalis (SO), Schizandra chinensis (SC), Caesalpinia sappan (CS) and Rhus javanica (RJ) exhibited high antimicrobial activities against E. coli, Minimum inhibitory concentrations (MIC) of the RJ extract against E. coli were 0.8 mg/ml. After heating treatment of these extracts, the antimicrobial activities against E. coli were significantly reduced in case of the CS extract. After alkaline or acid treatment of these extracts, the antimicrobial activities against E. coli were significantly increased in the PS extract but reduced in both SO and RJ extract. Since extracts from RJ and CS exhibited the highest antimicrobial activities, bacterial growth-inhibiting activities against E. coli by these two extracts were further examined. Optical density at 620 nm after 24 hours incubation of E. coli in the presence of 100, 300 or 500 ppm of RJ extract ranged from 0.1 to 0.2 compared to 0.35~0.65 in the absence of RJ extract, indicating that growth of E. coli was significantly inhibited within 24 hours by the addition of at least 100 ppm of RJ extract. Optical density at 620 nm after 24 hours incubation of E. coli in the presence of 300 or 500 ppm of CS extract ranged from 0.01 to 0.25 compared to 0.5~0.55 in the absence of CS extract, indicating that growth of E. coli was also significantly inhibited within 24 hours by the addition of at least 300 ppm of CS extract. In conclusion, these findings suggest that extracts from RJ and CS may play important roles for antimicrobial activities against E. coli causing various animal diseases.

• 한국식품위생안전성학회지
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• 제36권6호
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• pp.504-509
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• 2021

본 연구에서는 훈제오리 슬라이스에서 Escherichia coli 유통 중 생장 예측을 위한 dynamic model을 개발하였다. E. coli는 2개의 훈제 오리 시료(16.7%) 에서 1.23 log CFU/g검출되었다. 10-30℃ 보관에 따라 E. coli의 𝜇_max는 0.05-0.36 log CFU/g/h, LPD는 4.39-1.07h, h₀ 값은 0.24-0.51을 나타내었다. 개발된 모델의 검증은 15℃, 23℃에서 수행하였다. 모델 검증 결과 RMSE값이 0.130으로 개발된 모델이 다른 온도에 적용하기에 적합하다고 판단하였다. 이러한 결과는 E. coli로 개발된 모델은 훈제오리 슬라이스에서 E. coli의 변화하는 온도에 따른 생장을 예측하는 데 유용하다.