• Title/Summary/Keyword: Erwinia.sp.

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Isolation, identification, and culture conditions of the strain producing antibacterial antibiotic (항세균성 항생물질을 생산하는 미생물의 분리, 동정 및 배양조건)

  • Yoo, Jae-Hong;Yoon, Sang-Hong;Koo, Bon-Sung;Yeo, Yun-Soo;Park, In-Cheol;Lee, Byung-Moo;Ryu, Jin-Chang
    • The Korean Journal of Pesticide Science
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    • v.3 no.2
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    • pp.1-7
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    • 1999
  • The strain with antibacterial activity was isolated among soil samples collected in Suwon area. The isolated strain was identified as Bacillus sp. YR-1 with respect to its morphological, cultural, and physiological characteristics. Optimal medium for the highest production of antibiotic was composed of sucrose 2.0%(w/v), peptone 2.0%(w/v) and NaCl 0.1%(w/v). The maximum production of antibiotic was shown at $35^{\circ}C$ for 48 hours with the initial pH 7.0.

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Microflora of Daekwanryung Highland soil (대관령(大關嶺) 고령지(高嶺地) 토양(土壤)의 토양미생물상(土壤微生物相) 조사(調査))

  • Yun, Sei-Young;Kim, Jeong-Je;Yang, Jae-Wei;Jung, Young-Sang;Choi, Joong-Dae
    • Korean Journal of Soil Science and Fertilizer
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    • v.30 no.1
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    • pp.94-98
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    • 1997
  • This experiment was conducted to investigate the distribution of the soil microflora of Daekwanryung highlands of Kwangwon Province. It was found that soil microorganisms each as Bacteria, Actinomycetes, Fungi, Pseudomonas spp. and Erwnia sp. were mostly in the top 0-15 cm profile. It also was found that soil microflora population was affected in many ways by kind of cropping plant. The number of Erwinia sp. that is one of the soil born plant pathogenic bacteria was more abundant in potato field than chinese cabbage soil. It was also studied difference of mountain and grassland soils from cropping soils.

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Bacterial Chemotaxis to Extracts, Exudates, Solutions in Vitro and Soil (In vitro 및 토양에서 추출물, 삼출물, 용액에 대한 세균의 화학주성)

  • Lee Min Woon;Kim Sung Ill;Shim Jae Ouk;Shin Hyun Sung;Kim Gwang Po
    • Korean Journal Plant Pathology
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    • v.2 no.3
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    • pp.165-173
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    • 1986
  • Accumulation of Pseudomonas sp., P. fluorescens and Erwinia carotovora in 60 min treatment was greater in extracts from soil, exudate from ginseng root and solutions than distilled water. In bacterial movement toward rubber tube soil from chamber, accumulation of P. fluorescens in response to soil supplemented with soil extracts, exudate and solutions was generally greater in soil extracts compared to control and other solutions, but Pseudomonas sp. and E. carotovora were not much response to supplemented extracts, exudate and solutions. Accumulation of the bacteria in capillaries containing various exudates from fungal propagules with not attracted to the exudates. For an accumulation of bacteria in rubber tubes containing soil inoculated with fungal propagules, the Pseudomonas sp. was not attracted in soil inoculated by the organisms as attractant but P. fluorescens and E. carotovora to fungi were attracted to F. solani, F. oxysporum and mixed organism Alternaria panax did not affect on bacterial movement except E. carotovora. The organic matter conten in Kangwha and Kimpo soil were low in diseased and healthy soil. The K content was especially high in Kimpo healthy soil. Bacterial population in Goesan and Kangwha were more abundant than other soil. The number actinomycetes was populated abundant in healthy soil of Goesan and diseased soil of Poonggi.

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Research on Ginseng Diseases in Korea (인삼 병 연구의 과거와 현재)

  • 유연현;오승환
    • Journal of Ginseng Research
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    • v.17 no.1
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    • pp.61-68
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    • 1993
  • From the early 1,100 to 1,900, ginseng cultivation seemed to be practiced with management of the diseases which were, in general, referred to a "disaster" at that time. Farmers had individually developed their own methods to manage the disaster with a try and error from generations to generations. It was not determined until 1909, however, that plant pathogens as a new concept was involved in the disaster and thirteen ginseng diseases were reported in Korea by Japanese plant pathologists. The intensive researches have been carried out from 1978 when Korea Ginseng and Tobacco Research Institute was established. Among the ginseng diseases reported in ginseng growing countries, Altemaria Panax, Eotrytis sp., Cylindrocarpon destmctans, Colletotrichum gloeosporioides, Erwinia carotovora subsp. carotovora, Pythium app. Phytophthora cactomm, Sclerotinia sp., Sclerotium rolfsii, and root rot caused by nitylenchus destructor have been observed in Korea and the appropriate control methods for the major diseases were developed. However, the other nine diseases reported by former researchers have not been confirmed for their pathogenicities and/or occurrences on ginseng yetinseng yet.

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Effect of Thermophilic Bacteria on Degradation of Food Wastes (음식물 쓰레기 분해에 대한 고온성 미생물의 영향)

  • Yi, Hwe-Su;Jeong, Ji-Hyung;Park, Yu-Mi;Seul, Keyung-Jo;Ghim, Sa-Youl
    • Microbiology and Biotechnology Letters
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    • v.34 no.4
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    • pp.363-367
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    • 2006
  • Food wastes were decomposed into the Mugri (Isung Engineering, Korea), a food waste reduction machine, with adding sawdust of cryptomeria. Degradation effects were better when the machine worked at over 45$^{\circ}C$ than those at the lower temperature. Thermophilic bacteria were isolated from cryptomeria sawdust and the food waste products degraded by the machine. The isolates from cryptomeria sawdust were classified into 3 genera (Acinetobacter baumannii, Enterobacter sp. and Erwinia cypripedii) and almost all the isolates from the degraded products were partially identified as Bacillus sp. by 16S rDNA sequence analysis. The isolated thermophilic bacteria showed degradative enzyme activities. In the case of addition of the 30 thermophilic bacteria into the machine, degradation rate of food wastes was almost twice as high with increasing process temperature up to 6$^{\circ}C$.

Identification of Soft Rotting Bacteria of Solanaceae Vegetables in the Fields and Post-harvest (포장(圃場)과 수확(收穫) 후(後)에 가지과(科) 채소(菜蔬)에 무름병(病)을 일으키는 병원세균(病原細菌)의 동정(同定))

  • Choi, Jae Eul;Han, Kwang Sup
    • Korean Journal of Agricultural Science
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    • v.18 no.2
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    • pp.99-103
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    • 1991
  • The pathogenic bacteria isolated from the rotted fruits and tubers of red pepper, tomato, eggplant and potato which were collected from farms of Chungnam province and the markets of Seoul, Pusan, Taejon, Chonju, Iri, Weonju, Jecheon, Chungju and Cheonan in Korea. These isolates were divided into ninety-eight isolates of Enoinia sp. and seven isolates of Pseudomonas spp. on the basis of their taxonomic characteristics. Among seven isolates of Pseudomonas spp., six isolates were identified as P. viridiflava and two were P. marginalis and ninety-eight isolates of Erwinia sp. as E. carotovora. subsp. carotovora on the basis of bacteriological properties.

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Pathogenic bacteria causing rot in commercial soybean sprout cultivation

  • Yun, Sung-Chul;Kim, Yong-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.48 no.2
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    • pp.113-119
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    • 2003
  • Soybean sprout pathogenic bacteria were isolated from the large, deep containers of a commercial factory. Over a period of one year, 40 pathogenic-like bacteria were isolated among a total of 732 isolates. In addition to bacteria previously reported to be associated with rotting, such as Pseudomonas putida and Erwinia carotovora, several other genera were also identified: Acinetobacter spp., Chryseobacterium spp., Klebsiella sp., Pantoea agglomerans, Bacillus sp. Fatty acid methyl ester (FAME) analysis using the Microbial ID (MIDI) system, and 16s rRNA sequence analysis, yielded identical results, confirming the identities of these microorganisms. Several types of selective media were not good for identification and determination of population structure in commercial environments, as colony type was not specific to the genus. There was no dominant bacterium, and we were not able to find the main bacterium responsible for soybean spout rot. Even though we did not identify a major target for controlling rot or screening for resistant cultivars, the results of this study indicated that bacterial rot of soybean sprout is endemic. In addition, it emerged that factory epidemics in summer are not caused by the bacteria isolated in this study.

Ginseng Growths In Abolished Ginseng Fields and Factors Affecting the Ginseng Growth (폐포지 인삼 생육과 인삼 생육에 미치는 요인)

  • 김영호;이장호
    • Journal of Ginseng Research
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    • v.17 no.1
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    • pp.45-51
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    • 1993
  • In 61 abolished ginseng fields examined in 1986, the average plant missing was 49.2%, root rusting, 34.4%, and root rot, 3.3%. Plant missing was not different among the ages of ginseng fields; however, rate of root rusting was high in 3- and 4-year-old ginseng fields, and rate of root rot was high in 5- and 6-year-old ginseng fields, suggesting that root rusting and root rot were relatively more important factors related to the abolishment of ginseng filed in younger and older ginseng fields in 1986, respectively. Out of 61 ginseng fields, 18 were infested with more than 509) alternaria blights, and out of 19 fields with root rot, 2 were caused by Sclerotinia sp., 5 by potato rot nematode, and the causal agents were not identified in 12 fields. With increase of the ridge height, root rusting was significantly decreased, and plant missing rate was significantly lower in fields with straw mulching than those without mulching. The physico-chemical characteristics of the fields with more than 50fi root rusting, the contents of $NO_3,\;P_2O_5$ and Ca were higher than those of good fields with less than 40% root rusting and plant missing. The population of Erwinia sp. was significantly corralled with plant missing.

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Control of Powdery Mildew by Garlic Oil in Cucumber and Tomato (마늘오일을 이용한 오이와 토마토 흰가루병 방제)

  • Seo Sang-Tae;Lee Jung-Sup;Park Jong-Han;Han Kyoung-Suk;Jang Han-Ik
    • Research in Plant Disease
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    • v.12 no.1
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    • pp.51-54
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    • 2006
  • The effectiveness of four plant oils (garlic, ginger, cinnamon and lemongrass) against a range of plant pathogenic organisms was tested in vitro. Of the four oils, two oils (garlic and cinnamon) showed relatively good antimicrobial activities. Activity of garlic oil was shown against the plant pathogenic bacteria Agrobacterium tumefaciens, Ewinia carotovora, Ralstonia solanacearum, Xanthomonas campestris, and the fungi Phytophthora infestans, Fusarium oxysporum, Collectotricum sp., whereas Acidovorax avenae and Pythium sp. showed the resistance towards garlic oil. Results from the planta bioassays under greenhouse conditions indicated that garlic oil significantly reduced the cucumber powdery mildew (Sphaerotheca fusca) and tomato powdery mildew (Erysiphe cichoracearum) showing control value 70.0-74.6% and 71.2%, respectively.