• Title/Summary/Keyword: ErmS

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A Study on Implementation of the Integrated Electronic Resource Management System for Digital Librarian (디지털도서관 정보관리자를 위한 전자정보자원관리시스템 구축 연구)

  • Han Jong-Yup;Cho Hyun-Yang;Ki Myong-Jong
    • Journal of the Korean Society for Library and Information Science
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    • v.40 no.3
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    • pp.263-287
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    • 2006
  • With the rapid and broad changes of information media and information environment the traditional libraries have been evolving into the digital libraries. An analysis for the Planning and realization of the electronic resource management system was carried out to establish the various electronic resources in the digital library, the first in Korea. The study targeted from electronic journals, web-based databases, e-books, and to open access resources. The focus of the analysis lies in the Providing practical integrated electronic resource management for digital librarian based on the framework of effective Electronic Resource Management Initiative(ERMI) of Digital Library Federation(DLF) with network and electronic resource description. The analyses included life-cycle, subscription models, coverages. types of information provider for electronic resources. and system organization for implementation of electronic resource management system.

The Phosphorylation Status of Merlin Is Important for Regulating the Ras-ERK Pathway

  • Jung, Ju Ri;Kim, Hongtae;Jeun, Sin-Soo;Lee, Joo Yong;Koh, Eun-Jeoung;Ji, Cheol
    • Molecules and Cells
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    • v.20 no.2
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    • pp.196-200
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    • 2005
  • The neurofibromatosis type2 (NF2) tumor suppressor gene product, merlin, is structurally related to the ezrin-radixin-moesin (ERM) family of proteins that anchor the actin cytoskeleton to specific membrane proteins and participate in cell signaling. However, the basis of the tumor suppressing activity of merlin is not well understood. Previously, we identified a role of merlin as an inhibitor of the Ras-ERK signaling pathway. Recent studies have suggested that phosphorylation of merlin, as of other ERM proteins, may regulate its function. To determine whether phosphorylation of merlin affects its suppression of Ras-ERK signaling, we generated plasmids expressing full-length merlin with substitutions of serine 518, a potential phosphorylation site. A substitution that mimics constitutive phosphorylation (S518D) abrogated the ability of merlin to suppress effects of the Ras-ERK signaling pathway such as Ras-induced SRE transactivation, Elk-mediated SRE transactivation, Ras-induced ERK phosphorylation and Ras-induced focus formation. On the other hand, an S518A mutant, which mimics nonphosphorylated merlin, acted like wild type merlin. These observations show that mimicking merlin phosphorylation impairs not only growth suppression by merlin but also its inhibitory action on the Ras-ERK signaling pathway.

Fillers in the Hong Kong Corpus of Spoken English (HKCSE)

  • Seto, Andy
    • Asia Pacific Journal of Corpus Research
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    • v.2 no.1
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    • pp.13-22
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    • 2021
  • The present study employed an analytical framework that is characterised by a synthesis of quantitative and qualitative analyses with a specially designed computer software SpeechActConc to examine speech acts in business communication. The naturally occurring data from the audio recordings and the prosodic transcriptions of the business sub-corpora of the HKCSE (prosodic) are manually annotated with a speech act taxonomy for finding out the frequency of fillers, the co-occurring patterns of fillers with other speech acts, and the linguistic realisations of fillers. The discoursal function of fillers to sustain the discourse or to hold the floor has diverse linguistic realisations, ranging from a sound (e.g. 'uhuh') and a word (e.g. 'well') to sounds (e.g. 'um er') and words, namely phrase ('sort of') and clause (e.g. 'you know'). Some are even combinations of sound(s) and word(s) (e.g. 'and um', 'yes er um', 'sort of erm'). Among the top five frequent linguistic realisations of fillers, 'er' and 'um' are the most common ones found in all the six genres with relatively higher percentages of occurrence. The remaining more frequent realisations consist of clause ('you know'), word ('yeah') and sound ('erm'). These common forms are syntactically simpler than the less frequent realisations found in the genres. The co-occurring patterns of fillers and other speech acts are diverse. The more common co-occurring speech acts with fillers include informing and answering. The findings show that fillers are not only frequently used by speakers in spontaneous conversation but also mostly represented in sounds or non-linguistic realisations.

Design and Evaluation of a User Tag-based Retrieval Model for Electronic Journals within Electronic Resource Management Systems (전자자원관리시스템의 이용자 태그 기반의 전자저널 검색 모형 설계 및 평가에 관한 연구)

  • Kang, Jeong-Won;Kim, Hyun-Hee
    • Journal of the Korean Society for Library and Information Science
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    • v.43 no.4
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    • pp.241-264
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    • 2009
  • The study proposed an electronic journal retrieval model to be used to improve the retrieval efficiency of e-journals. To do this, firstly, we reviewed the literature on users' information behavior and on ERM (Electronic Resource Management) systems. Secondly, we conducted an e-mail survey of 96 participants (professors and graduate students) to find out about their information behavior on how to access, use and evaluate electronic resources as well as scientific information. Thirdly, we administered case studies on two ERMSs, Ex Libris' Verde and Innovative's Millennium. The proposed model will be operated within ERM systems and it enables to the supply of both system- and user-based services by combining taxonomy-based ERM systems with tag folksonomy. The model is unique in that it includes not only the automatic tagging functions that can be performed using log files but also the tag management functions including grouping similar or related tags.

Beneficial Effect of Korea Red Ginseng on Halitosis; Attenuation of H2S Induced Inflammatory Mediators and cystathionine γ-lyase Expression (고려홍삼의 구강악취 억제기능; H2S 생성에 따른 염증매개 유전자 및 cystathionine γ-lyase의 약화기능)

  • Choi, Ki-Seok;Lee, So-Jung;Lee, Jeong-Sang;Hong, Kyung-Sook;Kim, Jeong-Gon;Kim, Yoon-Jae;Hahm, Ki-Baik
    • Journal of Ginseng Research
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    • v.33 no.4
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    • pp.367-377
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    • 2009
  • Halitosis is a generally accepted marker of diseases in the oral cavity and of systemic and gastrointestinal disorders. Based on these authors' previous findings (that (1) there is a close association between H. pylori infection and halitosis; (2) Korea red ginseng may suppress the colonization of H. pylori, fight H. pylori-induced cytotoxicity, and impose significant anti-inflammatory actions in patients with chronic gastritis; and (3) H. pylori infection is linked with the generation of significant levels of volatile sulfur compounds (VSCs), and the levels of VSCs correlate significantly with H. pylori-associated mucosal damages), in the current study, the authors documented the molecular mechanisms of Korea red ginseng's efficacy in ameliorating halitosis. When the RAW 264.7 cells were treated with the $H_2S$ releasing compound NaHS, the mRNA expression of cystathionine ${\gamma}$-lyase (CSE), IL-6, COX-2, and iNOS were more significantly induced compared with the vehicle-treated group. The cytoskeletal components of ezrin's and moesin's mRNA expressions were elevated by NaHS treatment accompanied by the activation of MAPK, p38, and ERK. Korea red ginseng pretreatment reduced both the NaHS-induced CSE expression and the proinflammatory genes (e.g., IL-6, COX-2, and iNOS) in a concentration-dependent manner. The ERM expression and the phosphorylation of p38 were also significantly reduced by Korea-red-ginseng pretreatment. Overall, Korea red ginseng pretreatment imposed significant anti-inflammatory effects through the downregulation of the NaHS-triggered proinflammatory gene expression, CSE, and ERM mRNA expression. Korea red ginseng could thus be said to be a key remedy of halitosis and to be effective in relieving gastric inflammation.

PERIODONTOPATHIC BACTERIA AND ANTIBIOTIC RESISTANCE GENES OF ORAL BIOFILMS IN CHILDREN (어린이 치면세균막에서 치주질환원인균과 항생제 내성유전자의 출현율)

  • Kim, Seon-Mi;Choi, Nam-Ki;Cho, Seong-Hoon;Lee, Seok-Woo;Lim, Hoi-Jeong;Lim, Hoi-Soon;Kang, Mi-Sun;Oh, Jong-Suk
    • Journal of the korean academy of Pediatric Dentistry
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    • v.38 no.2
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    • pp.170-178
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    • 2011
  • The purpose of this study was to assess the prevalence of periodontopathic bacteria and resistance determinants from oral biofilm of children. Subgingival dental plaque was isolated from 87 healthy children, and PCR was performed to determine the presence of 5 periodontal pathogens including P. gingivalis, T. forsythia, T. denticola, F. nucleatum, A. actinomycetemcomitans, and nine resistance genes including tet(Q), tet(M), ermF, aacA-aphD, cfxA, $bla_{SHV}$, $bla_{TEM}$, vanA, mecA. 1. The prevalence of F. nucleatum, T. forsythia. and P. gingivalis was 95.4%, 55.2%, and 40.2%, respectively. In addition. the prevalence of A. actinomycetemc omitans was 5.7%, while T. denticola was 3.4%. 2. In analysis of antibiotic resistance determinants. cfxA, $bla_{TEM}$ and tet(M) were detected in all the samples tested. It was also found that the prevalence of tet(Q) showing tetracycline resistance. $bla_{SHV}$ associated with resistance to ${\beta}$-lactams, ermF exhibiting erythromycin resistance, and, vanA resulting vancomycin resistance was 88.5%, 29.9% 87.4%, and 48.5%, respectively. The aacA-aphD gene showing resistance to aminoglycosides and mecA gene harboring methicillin resistance exhibited the lowest prevalence with 9.2%. 3. In a correlation analysis between periodontopathic pathogens and antibiotic resistance determinants, it was found that there was a significant correlation between T. forsythia and $bla_{SHV}$. Also, P. gingivalis and vanA showed a correlation. Finally, tet(Q) and ermF showed a significant correlation (phi: 0.514) while mecA and vanA also showed a correlation(phi: 0.25).

Time Series Observations of Atmospheric Radon Concentration in Seoul, Korea for an Analysis of Long-Range Transportation of Air Pollutants in the North-East Asia (동북아 오염물질 장거리이동 분석을 위한 서울시 대기 중 라돈농도의 시계열적 특성에 관한 연구)

  • Kim, Yoon-Shin;Lee, Cheol-Min;Kim, Ki-Youn;Jeon, Hyung-Jin;Kim, Jong-Cheol;Iida, Takao
    • Journal of Environmental Health Sciences
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    • v.33 no.4
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    • pp.283-292
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    • 2007
  • Atmospheric concentrations of radon had been continuously observed in Seoul, Korea since December 1999, as a tracer for long-range transport of air pollutants from China continent to Korea. In order to study radon as a tracer of long-range transport, it is important to know information about the atmospheric distribution and variation of radon concentration and its time variation. Atmospheric radon concentration are measured with electrostatic radon monitor(ERM) at Hanyang University located in Eastern area of Seoul. Air sample is taken into a vessel of ERM, and alpha particles emitted by radon daughters $Po^{218}$ are detected with ZnS(Ag) scintillation counter. Hourly mean concentrations and hourly alpha counts are recorded automatically. The major results obtained from time series observation of atmospheric radon were as follows : (1) The mean of airborne radon concentration in Seoul was found to be $7.62{\pm}4.11\;Bq/m^3$ during December $1999{\sim}January$ 2002. (2) The hourly variation of radon concentrations showed the highest in 8:00AM ($8.66{\pm}4.22\;Bq/m^3$) and the lowest in 3:00AM ($6.62{\pm}3.70\;Bq/m^3$) and 5:00AM ($6.62{\pm}3.39\;Bq/m^3$). (3) the seasonal variation of radon concentrations showed higher during winter-to-fall and lower during summer-to-spring. (4) Correlation between airborne radon concentration and the meteorological factors were -0.21 for temperature, 0.09 for humidity, -0.20 for wind speed, and 0.04 for pressure. (5) The mean difference of airborne radon concentration between Asian dust ($5.36{\pm}1.28\;Bq/m^3$) and non-Asian dust ($4.95{\pm}1.49\;Bq/m^3$) phenomenon was significant (p=0.08). We could identify time series distribution of radon concentration related meteorological factors. In addition, radon can be considered a good natural tracer of vertical dispersion and long-range transport.

Expression Profiles of Streptomyces Doxorubicin Biosynthetic Gene Cluster Using DNA Microarray System (DNA Microarray 시스템을 이용한 방선균 독소루비신 생합성 유전자군의 발현패턴 분석)

  • Kang Seung-Hoon;Kim Myung-Gun;Park Hyun-Joo;Kim Eung-Soo
    • KSBB Journal
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    • v.20 no.3
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    • pp.220-227
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    • 2005
  • Doxorubicin is an anthracycline-family polyketide compound with a very potent anti-cancer activity, typically produced by Streptomyces peucetius. To understand the potential target biosynthetic genes critical for the doxorubicin everproduction, a doxorubicin-specific DNA microarray chip was fabricated and applied to reveal the growth-phase-dependent expression profiles of biosynthetic genes from two doxorubicin-overproducing strains along with the wild-type strain. Two doxorubicin-overproducing 5. peucetius strains were generated via over-expression of a dnrl (a doxorubicin-specific positive regulatory gene) and a doxA (a gene involved in the conversion from daunorubicin to doxorubicin) using a streptomycetes high expression vector containing a strong ermE promoter. Each doxorubicin-overproducing strain was quantitatively compared with the wild-type doxorubicin producer based on the growth-phase-dependent doxorubicin productivity as well as doxorubicin biosynthetic gene expression profiles. The doxorubicin-specific DNA microarray chip data revealed the early-and-steady expressions of the doxorubicin-specific regulatory gene (dnrl), the doxorubicin resistance genes (drrA, drrB, drrC), and the doxorubicin deoxysugar biosynthetic gene (dnmL) are critical for the doxorubicin overproduction in S. peucetius. These results provide that the relationship between the growth-phase-dependent doxorubicin productivity and the doxorubicin biosynthetic gene expression profiles should lead us a rational design of molecular genetic strain improvement strategy.

Development of a Recombinant Streptomyces griseus with sprA and sprB Genes for Proteolytic Enzyme Production (Streptomyces griseus IFO13350 유래 sprA 및 sprB 유전자를 이용한 Pretense 생산균주 개발)

  • Hwang Ji-Hwan;Lee Chang-Kwon;Lee Kang-Mu;Jo Byoung-Kee;Park Hae-Ryong;Hwang Yong-Il
    • Korean Journal of Microbiology
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    • v.41 no.1
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    • pp.87-92
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    • 2005
  • Pronase, a protease produced for commercial purpose by Streptomyces griseus, was composed of serine protease, alkaline protease, aminopeptidase and carboxypeptidase complex, and it has been widely used as anti-inflammatory drugs for human therapy. In this study, we developed a new integration vector, pHJ101 derived from pSET152, containing strong promoter, ermE, to overexpress a certain protease gene. Specific PCR primers for cloning of sprA (a gene for S. griseus protease A) and sprB (a gene for S. griseus protease B) genes were designed from the basis of nucleotide sequence in databases and amplified by PCR. Plasmid pHJ201 and pHJ202 were constructed by inserting of amplified each gene in a vector pHJ101. S. griseus HA and S. griseus HB were respectively obtained by conjugal process of a parent strain, S. griseus IFO 13350 with the recombinant Escherichia coli harboring plasmid pHJ201 or pHJ202. When protease activity was measured in flask cultivation, produced protease levels of S. griseus HA and S. griseus HB increased about 5.3 times and 5 times, respectively, more than that of parent strain. And, the constructed integrating plasmid pHJ101 was applicable for overexpression of a certain gene in Streptomyces sp.