• 한국응용과학기술학회지
• /
• 제25권4호
• /
• pp.533-538
• /
• 2008

In order to fine antioxidant and whitening agent source from nature, the comparisons of antioxidative activity and tyrosinase inhibitory activity were carried out for various ethanol extract on Polygoni Multiflori Radix, Polygonati Rhizoma and Ephedrae Herba. Comparing for three ethanol extracts, the highest electron donating ability was found at Polygonati Rhizoma (86.6%), but, the highest SOD-like ability, at the Ephedrae Herba (47.8%). Xanthine oxidase experiment exhibited 95.7% of hindrance effect in Ephedrae Herba, and 84.0% in Polygonati Rhizoma. A tyrosinase inhibitory activity assay was conducted to evaluate the whitening effects of the extracts, The tyrosinase inhibitory activity was 6.5% in the Polygoni Multiflori Radix, 32.6% in the Polygonati Rhizoma, 64.0% in the Ephedrae Herba. Based on these results, we suggest that the ethanol extracts of Polygoni Multiflori Radix, Polygonati Rhizoma and Ephedrae Herba can be used as food and cosmetic ingredients.

• 대한약침학회지
• /
• 제10권2호통권23호
• /
• pp.41-46
• /
• 2007

Effects of mahwang(Ephedrae herba) aqua-acupuncture at sinsoo (B-23)and Jisil(B-52)on adrenal cortical insufficiency were investigated in dexamethasone treated rats. Concentration of serum cortisol was decreased in dexamethasone treated rats. However, these values showed a tendency to increase in mahwang(Ephedrae herba) aqua-acupuncture groups. Concentration of serum total protein was increased in dexamethasone treated rats. However, these values were decreased by the mahwang(Ephedrae herba) aqua-acupuncture. The portion of neutrophils was decreased and the portion of lymphocytes and eosinophils were increased in dexamethasone treated rats. However, in mahwang(Ephedrae herba) aqua-acupuncture groups, the portion of neutrophils showed a tendency to increase and the portion of lymphocytes and eosinophils showed a tendency to decrease. In dexamethasone treated rats, the weight of adrenal glands were decreased, however these values were increased in mahwang(Ephedrae herba) aqua-acupuncture groups.

• 사상체질의학회지
• /
• 제13권1호
• /
• pp.79-87
• /
• 2001

In order to investigate the effect of Taeumjowetang and Herba Ephedrae on obesity-induced rats, after Taeumjowetang and Herba Ephedrae were administered by mouth, and then body-weight, serum triglyceride, total cholesterol, Obese mRNA and TNF-${\alpha}$/ mRNA were measured. The reselts were as follows ; 1. The body-weight was decresed with the statistical significance in both the Taeumjowetang and the Herba Ephedrae group as compared with the control group. 2. The quantitation of serum triglyceride was decresed with the statistical significance in both the Taeumjowetang and the Herba Ephedrae group as compared with the control group. 3. The total cholesterol was slightly decresed in both the Taeumjowetang and the Herba Ephedrae group as compared with the control group, but there was no statistical significance. 4. The manifestation of Obese mRNA and TNF-${\alpha}$/ mRNA in epidymal, retroperitoneal, mesenteric and skeletal muscle tissues were inhibited in Taeumjowetang and Herba Ephedrae group. According to the above results, Taeumjowerang and Herba Ephedrae were recognized to be helpful for the treatment of obesity.

• 대한한의학방제학회지
• /
• 제13권2호
• /
• pp.169-192
• /
• 2005

This report describes the remedial fields, symptoms, pathlogy, dosage, prescriptional constitution of 79 studies to the use of Herba Ephedrae main blended prescriptions from dongeubogam. The following conclusions were reached through investigations on the prescriptions that use Herba Ephedrae as a key ingredient Herba Ephedrae blended prescriptions are utilized for 26 therapeutic purposes, for example, in symptoms of cough, cold, wind. In particular, 20.2% of the prescriptions appear in the chapter of cough. Prescriptions that utilize Herba Ephearae as the main ingredient are used in the treatment of 47 different types of diseases, for example, in the treatment of wind-cold pathogen, cold, wind, exogenous febrile disease etc. Herba Ephedrae is most widely used in six pathogenic factors such as wind, cold, dampness, heat. The actual amount of Herba Ephedrae blended has ranged at a wide variety of amounts from 1 don to 2 don. It is widely applied with base prescriptions such as Mahwang-tang, Samyo-tang, Mahwangbujasesin-tang, Mahwangkanghwal-tang.

• 대한약침학회지
• /
• 제10권2호통권23호
• /
• pp.57-65
• /
• 2007

Objectives : The purpose of this study was to investigate the antioxidative effects of Ephedrae Herba extract. Methods : Total antioxidant status was examined by total antioxidant capacity(TAC), total antioxidant response(TAR) against potent free radical reactions, total phenolic content, DPPH radical scavenging activities, and the inhibitory effect of the extract on lipid peroxidation in rat liver mitochondria. Results : 1. TAC and TAR of Ephedrae Herba extract at the concentration of 5mg/ml were 3.76 and 1.65 mM Trolox equivalents , respectively. 2. Total phenolic content of Ephedrae Herba extract at the concenuation of 5mg/ml was 3.52 mM gallic acid equivalent. 3. Concentration of Ephedrae Herba extract at which DPPH radical scavenging activity was inhibited by 50% was 0.46mg/ml as compared to 100% by pyrogallol solution as a reference. 4. Ephedrae Herba extract at the concentration of 2mg/ml significantly decreased TBARS concentration by 97%, showing that Ephedrae Herba extract prevented lipid peroxidation at the low concentration. Conclusion : Thus antioxidant effects of Ephedrae Herba extract appear to be due to, at least in part, the prevention from free radicalsinduced oxidation, followed by inhibition of lipid peroxidation.

• 한방안이비인후피부과학회지
• /
• 제22권2호
• /
• pp.19-38
• /
• 2009

Objective : The purpose of this study is to find out whether Herba Ephedrae, solely used, and Herbal medicine in which this was included, have inhibitory effects of Nitric Oxide(NO). Methods : We tested the inhibitory effects of Nitric Oxide(NO) with Herba Ephedrae and ten kinds of Herbal medicine combined with Herba Ephedrae(Worlbikachul-Tang, 越婢加朮湯; Mahaengkamsuk-Tang, 麻杏甘石湯; Shinbi-Tang, 神秘湯; Mahwangbujaseshin-Tang, 麻黃附子細辛湯; Euiin-Tang, 薏苡仁湯; Galgeun-Tang, 葛根湯; Mahaengeuigam-Tang, 麻杏薏甘湯; Mahwang-Tang, 麻黃湯; Socheongryong-Tang, 小靑龍湯; Gaemagakban-Tang, 桂麻各半湯) on RAW264.7 cells. Results and Conclusions : 1. We carried out MTT assay on Herba Ephedrae and those decoctions including this in order to determine whether they accommodate cotytoxicity. The results were that Worlbikachul-Tang, Mahaengkamsuk-Tang, Mahwangbujaseshin-Tang, Mahaengeuigam-Tang, Mahwang-Tang, Socheongryong-Tang and Gaemagakban-Tang showed no cytotoxicity on RAW264.7 with 0.1mg/ml and 0.5mg/ml dosages of decoctions but displayed cytotoxicity on the cell with 1mg/ml. Solely used Herba Ephedrae, Shinbi-Tang, Euiin-Tang and Galgeun-Tang exhibited cytotoxicity beyond the concentration of 0.5mg/ml. 2. Worlbikachul-Tang, Mahaengkamsuk-Tang, Shinbi-Tang, Mahwangbujaseshin-Tang, Euiin-Tang, Galgeun-Tang, Mahaengeuigam-Tang, Mahwang-Tang and Socheongryong-Tang showed inhibition of NO production but solely used Herba Ephedrae and Gaemagakban-Tang did not exhibit such reaction.

• 한국자원식물학회지
• /
• 제22권5호
• /
• pp.431-437
• /
• 2009

in vivo 및 in vitro에서 마황의 항염증효과를 검토하기 위하여 마황추출물을 급여한 흰쥐에게 LPS shock로 급성기 염증반응을 유발시킨 후, 혈액 및 간장의 전염증성 cytokines들의 농도를 경시적으로 조사하였으며, 한편으로는 Raw 264.7 cell에 LPS shock를 가한 후, 마황추출물이 각종 전염증성 cytokines들의 생산량에 미치는 영향을 조사했다. in vivo 실험에서, 각 처리군 별 plasma IL-$1{\beta}$, IL-6, TNF-$\alpha$ 및 IL-10 농도의 경시적 변동은 전 처리군 모두가 LPS 처리 후, 2h째 급격하게 증가하여, 5h째에 최고치를 나타내었다. Plasma IL-$1{\beta}$, IL-6 및 TNF-$\alpha$의 농도는 LPS처리 후 5h째에서 마황 첨가군 모두가 대조군보다 낮은 값을 나타내었다. Plasma IL-10의 농도는 LPS처리 후, 2h째 및 5h째 모두 에서 마황추출물 첨가군 들이 대조군보다 높은 값을 나타내었다. Raw 264.7 macrophages를 이용한 in vitro 실험에서, IL-$1{\beta}$, IL-6 및 TNF-$\alpha$의 농도들은 대조군보다 마황처리 군들 모두가 낮은 값을 나타내었다. IL-10의 농도는 대조군보다 마황처리군들이 다소 높은 경향을 보였으나, 유의한 차이는 아니었다. 이상의 결과들을 종합해보면 마황에 내재하는 기능성 물질들이 염증반응을 완화하는 효과를 가지고 있음을 시사해 준다.

• 약학회지
• /
• 제42권4호
• /
• pp.422-430
• /
• 1998

In order to study the effects of Rhei rhizoma, Ephedrae herba and Scutellariae radix on hepatic metabolism, we examined the pretreatment effect of those on the metabolism of 7-e thoxycoumarin (EC). Water extracts (1g/kg) of Rhei rhizoma, Ephedrae herba and Scutellariae radix were administered orally to rats for 7 days, respectively. Livers were then isolated and perfused with 100mcM EC for 2 hours. The metabolites of EC, 7-hydroxycoumarin, sulfate conjugate and glucuronide conjugate were measured in the perfusates. The amount of glucuronide conjugates was decreased in Rhei rhizoma pretreated rats (p<0.01), however, 7-hydroxycoumarin was increased in Ephedrae herba pretreated rats (p<0.01). We examined whether the change of enzyme activity is related to the change of cytochrome P4501A1 and P4502B1 mRNA level in the perfused rat liver, which are responsible for EC metabolism. CYP1A1 and CYP2B1 mRNA level was increased, which was was not statistically significant with rhei rhizoma nor ephedrae herba pretreatment. We also assessed the hepatotoxicity of Rhei rhizoma, Ephedrae herba and Scutellariae radix. The activities of ALT and AST were assayed at 24 hours after 7 days administration. Only the ratio of ALT over AST was increased in ephedrae herba pretreated rats (p<0.05). Lipid peroxidation was increased in Rhei rhizoma treatment (p<0.05), while histopathological examination performed after liver perfusion did not show any difference compared with vehicle treatment. These results suggest that Ephedrae herba pretreatment increases the o-deethy-lation of 7-ethoxycoumarin in rats, which may be mediated by CYP1A1 mRNA induction.

• 한국자원식물학회지
• /
• 제19권2호
• /
• pp.296-299
• /
• 2006

마황 추출액이 흰쥐의 혈액내 지질구성, 간장기능 및 혈구성상에 미치는 영향을 검토했다. 혈장내 FFA 농도는 처리군 간에 유의한 차이를 나타내지 않았다. 그러나 triglyceride 농도는 마황 첨가량이 증가함에 따라 감소하였다. 총콜레스테롤량은 마황 첨가군에서 현저하게 감소했다. 그러나 HDL 및 LDC-cholesterol 농도는 처리군 간에 유의한 차이를 나타내지 않았다. GOT 활성치는 마황 첨가군에서 높은 경향을 보였으나, GPT 활성치는 처리군 간에 유의한 차이를 나타내지 않았다. RBC는 마황 첨가군에서 높은 수치를 보였으나, Hb 및 PCV는 처리군 간에 유의한 차이를 보이지 않았다. WBC 총수는 처리군 간에 유의한 차이를 나타내지 않았다. 백혈구 구성비는 neutrophils의 비율이 마황 첨가군에서 증가하는 경향을 보였으나 나머지 종류들은 처리군 간에 유의한 차이를 나타내지 않았다.

• 대한본초학회지
• /
• 제23권4호
• /
• pp.1-7
• /
• 2008

Objectives: Ephedrae herba, also known as Ma-huang, is a traditional Korean medicinal herb. It has been used to treat asthma, nose and lung congestion, and fever with anhidrosis for centuries. Recently, Ma-huang was used as a source of ephedrine in many dietary supplements for weight reduction in the United States. The objective of this study was to investigate the subacute toxicity of ephedrae herba extract in rats. Methods: SPF Sprague-Dawley male rats were administered orally with ephedrae herba extract for 4 weeks as several doses(0, 125, 250, 500, 1,000, and 2,000 mg/kg). We examined number of deaths, clinical signs, body weights and gross findings for experimental period. Results: No dead animals were found during the experimental period. In addition, no differences were found between control and treated groups in clinical signs, hematology, serum biochemistry, and other findings. Conclusions: In conclusion, above data suggest that no observed adverse effect level of ephedrae herba extract in SD rats might be over 2,000 mg/kg/day in this study.