• Food Science and Biotechnology
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• 제16권5호
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• pp.663-674
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• 2007

Starches from different botanical sources differ in the ratio of amylose to amylopectin contents, molecular structures of amylose and amylopectin, granule morphology, and minor-component contents. These structural features result in different gelatinization, pasting, retrogradation properties, and enzyme digestibility of starch granules. In this review, compositions and molecular structures of starches and their effects on the physicochemical properties are summarized and discussed.

• Asian-Australasian Journal of Animal Sciences
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• 제15권5호
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• pp.708-712
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• 2002

Effects of unprocessed (whole) or steam-flaked corn with or without enzyme additives on in vivo nutrient digestibilities and distribution of corn particles in the feces of Holstein steers were determined in a $4{\times}4$ Latin square experiment using four Holstein steers fed the diets containing 1) whole corn without enzyme additive, 2) whole corn with enzyme additive, 3) flaked corn without enzyme additive, or 4) flaked corn with enzyme additive. With regard to nutrient digestibilities such as DM, CP, CF, NFE, NDF, and ADF, no significant differences were detected among treatments, and also the nutrient digestibilities were not affected by the addition of enzyme additive. When distribution of corn particles in the feces was examined, there were no significant differences in the amount of 2, 8 mm and total corn particles. However, feeding flaked corn resulted in less corn particles (4 mm) in the feces than feeding whole corn (p<0.05). There were no significant differences in amounts of corn particles in the feces due to the addition of enzyme additive.

• Asian-Australasian Journal of Animal Sciences
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• 제29권4호
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• pp.539-548
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• 2016

The objective of the present study was to investigate the influence of palm kernel meal (PKM) inclusion and exogenous enzyme supplementation on growth performance, nitrogen-corrected apparent metabolizable energy (AMEn), coefficient of apparent ileal digestibility (CAID) and total tract retention of nutrients in young broilers fed corn-based diets. Four inclusion levels of PKM (no PKM [PKM0], 8% [PKM8], 16% [PKM16], and 24% [PKM24]) and two enzyme additions were evaluated in a $4{\times}2$ factorial arrangement of treatments. A total of 384, one-d-old male broilers (Ross 308) were individually weighed and allocated to 48 cages (eight broilers/cage), and cages were randomly assigned to eight dietary treatments. Results indicated that the inclusion of 8% and 16% PKM increased (p<0.05) the weight gain compared to the PKM0 diet. Birds fed the PKM8 diets had the highest (p<0.05) feed intake. Weight gain and feed intake were severely reduced (p<0.05) by feeding the PKM24 diet. Enzyme supplementation increased weight gain (p<0.05), independent of PKM inclusion level. In PKM0 and PKM8 diets, enzyme addition significantly (p<0.05) lowered feed conversion ratio (FCR); whereas enzyme addition had no effect on FCR of birds fed PKM16 and PKM24 diets. In PKM0 and PKM16 diets, enzyme addition significantly (p<0.05) increased CAID of nitrogen and energy but had no effect in the PKM8 and PKM24 diets. Inclusion of PKM into the basal diet, irrespective of inclusion level, enhanced (p<0.05) starch and fat digestibility. Inclusion of PKM at 16% and 24% resulted in similar CAID of neutral detergent fiber (NDF) but higher (p<0.05) than that of the PKM0 and PKM8 diets. Enzyme addition, regardless of the level of PKM inclusion, significantly (p<0.05) increased CAID of NDF. There was a significant (p<0.05) decrease in AMEn with PKM inclusion of 24%. The present data suggest that inclusion of PKM in broiler diets could be optimized if PKM-containing diets are formulated based on digestible amino acid contents and supplemented with exogenous enzymes. If amino acid digestibility and AME of PKM considered in the formulation, it can be included in broiler diets up to 16% with no deleterious effects on growth performance.

• Asian-Australasian Journal of Animal Sciences
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• 제16권2호
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• pp.248-256
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• 2003

Two experiments were conducted to determine the effects of expander processing and enzyme supplementation of wheat-based diets on growth performance and nutrient digestibility in finishing pigs. For Exp. 1, 60 finishing pigs (average initial BW of 49.5 kg) were fed meal, standard pellets and expanded pellets in a 70 d growth assay. From 49.5 to 79.0 kg, 79.0 to 111.8 kg, and overall (49.5 to 111.8 kg), ADG and ADFI were not affected by pelleting or standard vs expander conditioning (p>0.22). However, from 49.5 to 79.0 kg, pigs fed pellets have greater gain/feed than pigs fed mash (p<0.04), and pigs fed expanded pellets tended to have greater (p<0.10) gain/feed than pigs fed standard pellets. Overall (i.e. from 49.5 to 111.8 kg), gain/feed (p<0.02) and apparent fecal digestibilities of DM (p<0.001) and N (p<0.02) were improved by pelleting the diets. Also, expander processing further improved gain/feed (p<0.06) and digestibility of DM (p<0.04) compared to standard steam conditioning. Scores for keratinization (p<0.002) and ulceration (p<0.003) of the stomach were increased by pelleting, but the mean scores for the various treatments ranged only from 0.05 to 1.08 (i.e., low to mild keratosis and ulceration). For Exp. 2, 80 pigs (average initial BW of 54.1 kg) were fed mash and pellets (standard or expander) without and with xylanase. The enzyme was added to supply 4,000 units of xylanase activity/kg of diet. Adding xylanase to the mash diet improved gain/feed from 90.7 to 115.9 kg (p<0.04) of the growth assay and digestibility of DM (p<0.05) on d 39. However, in pelleted diets, adding the enzyme did not improve growth performance or digestibility of nutrients. Pelleting tended to increase scores for ulceration (p<0.06), and enzyme supplementation decreased stomach keratinization scores for pigs fed the standard pellets (p<0.01). However, as in Exp. 1, the mean scores for all treatment groups were quiet low (i.e., ranging from normal to mild). In conclusion, pelleting improved efficiency of growth, but additional benefits from expander conditioning were observed only in Exp. 1. Finally, xylanase tended to improve growth performance and nutrient digestibility, only in pigs fed mash diets but not in pigs fed pellets.

• Asian-Australasian Journal of Animal Sciences
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• 제14권10호
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• pp.1434-1439
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• 2001

Eighty crossbred pigs (Large White x Landrace) weighing 9.9 kg were assigned on the basis of sex, weight and Jitter to one of five dietary treatments in a factorial $(5\;treatments\;{\times}2\;sexes)$ arrangement to compare the nutritive value of hulled and dehulled peas fed with or without enzyme (0.25% Allzyme PF and 0.5% Biogal-S). A barley and soybean meal diet served as a control. Eight castrates and eight gilts were fed each diet. Digestibility coefficients for dry matter, crude protein and energy were higher for diets containing dehulled peas than hulled peas. In addition, enzyme supplementation modestly increased the digestibility of all three nutrients. Over the entire experimental period (9.9 to 103.3 kg), there were no performance differences (p>0.05) between pigs fed soybean meal based diets or diets based on any of the pea products. In addition, there were no differences in performance between pigs fed diets containing hulled or dehulled peas or between pigs fed diets with or without dietary enzyme. Castrates gained weight significantly faster, consumed more feed but had a poorer feed conversion than gilts (p<0.05). There were no differences in carcass traits between pigs fed diets based on soybean meal or any of the pea products. Carcass traits were similar for pigs fed hulled or dehulled peas while enzyme supplementation also had no effect on carcass data. Castrate pigs had a lower carcass value index, estimated lean yield and loin lean depth (p<0.05). Loin fat depth was greater for castrates than gilts (p<0.05). The overall results of this experiment provide little support for the need for enzyme supplementation of pea based diets fed to swine. In addition, dehulling did not appreciably improve the nutritive value of peas. Therefore, since the process adds to the cost of the raw product, its use is unlikely to be economical.

• Journal of Animal Science and Technology
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• 제65권1호
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• pp.32-56
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• 2023

This review explores the factors that improve meat protein digestibility and applies the findings to the development of home meal replacements with improved protein digestion rates in older adults. Various methods improve the digestion rate of proteins, such as heat, ultrasound, high pressure, or pulse electric field. In addition, probiotics aid in protein digestion by improving the function of digestive organs and secreting enzymes. Plant-derived proteases, such as papain, bromelain, ficin, actinidin, or zingibain, can also improve the protein digestion rate; however, the digestion rate is dependent on the plant enzyme used and protein characteristics. Sous vide processing improves the rate and extent of protein digestibility, but the protein digestion rate decreases with increasing temperature and heating time. Ultrasound, high pressure, or pulsed electric field treatments degrade the protein structure and increase the proteolytic enzyme contact area to improve the protein digestion rate.

• Asian-Australasian Journal of Animal Sciences
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• 제29권7호
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• pp.998-1003
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• 2016

Although exogenous protease enzymes have been used in poultry diets quite extensively, this has not been the case for pig diets. In general, due to their better gut fermentative capacity and longer transit time, pigs have greater capacity to digest dietary proteins than poultry. However, in early-weaned piglets, the stress brought about by weaning adversely affects the digestion of dietary proteins. Therefore, a study was conducted to determine the effects of a commercial protease enzyme in weanling pigs. Indices of growth, nutrient digestibility, blood profiles, fecal microflora, fecal gas emission and fecal scores were measured during the study. A total of 50 weanling pigs ($6.42{\pm}0.12kg$) at 28 d of age were randomly assigned to receive 1 of 2 dietary treatments: i) control diet (corn-soy based) with no supplemental protease (CON), and ii) control diet+200 g/ton protease (PROT) for 42 d. A completely randomized design consisting of 2 treatments, 5 replicates, and 5 pigs in each replicate was used. Growth performance in terms of body weight ($27.04{\pm}0.38kg$ vs $25.75{\pm}0.39kg$; p<0.05) and average daily gain ($491{\pm}7.40g$ vs $460{\pm}7.46g$; p<0.05) in PROT fed pigs were increased significantly, but gain per feed ($0.700{\pm}0.01$ vs $0.678{\pm}0.01$; p>0.05) was similar between treatments at d 42. Relative to CON pigs, PROT fed pigs had increased (p<0.05) apparent total tract digestibility ($84.66%{\pm}0.65%$ vs $81.21%{\pm}1.13%$ dry matter and $84.02%{\pm}0.52%$ vs $80.47%{\pm}1.22%$ nitrogen) and decreased (p<0.05) $NH_3$ emission ($2.0{\pm}0.16ppm$ vs $1.2{\pm}0.12ppm$) in the feces at d 42. Except for a decreased (p<0.05) in blood creatinine level, no differences were observed in red blood cell, white blood cell, lymphocyte, urea nitrogen, and IgG concentrations between treatments. Fecal score and fecal microflora (Lactobacillus and E. coli) were also similar between CON and PROT groups. Overall, the supplementation of protease enzyme in weanling pigs resulted in improved growth rate and nutrient digestibility. Exogenous protease enzyme reduced fecal $NH_3$ emission, thus, potentially serving as a tool in lowering noxious gas contribution of livestock production in the environment.

• Preventive Nutrition and Food Science
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• 제3권2호
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• pp.180-185
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• 1998

A regression equation was proposed for predicting protein digestibility using pH drop and free amino acid content. Results were compared with those determined by the pH drop method of Satterle et al. and with apparent in vivo digestibility in rats. Measurd free amino acid content prior to four enzyme digestion had an influence on calculating digestbiilty . Results from new equation correlated more highly (r=0.8434, difference average=2.304) with in vivo digestibility than the results of pH drop method (r=0.7603, difference average=10.099).

• Journal of Animal Science and Technology
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• 제53권2호
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• pp.113-118
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• 2011

본시험은 복합효소제 첨가 급여에 따른 육성돈의 생산성, 영양소소화율, 혈액성상 및 사료비용에 관하여 평가하였다. 시험 동물은 3원교잡종 [(Landrace ${\times}$ Yorkshire) ${\times}$ Duroc] 육성돈 96두를 공시하여 6주간 실시하였고, 시험 개시체중은 $22.96{\pm}0.79$kg이었다. 시험 사료는 고에너지 사료와 대조구 사료를 이용하여 고에너지 사료(HC) 처리구, 일반 사료(CON)인 대조구, 대조구 사료에 복합효소제 0.05%와 0.1%을 첨가한 CE1과 CE2로 4처리구 각 처리당 4마리씩 6반복으로 완전임의 배치하여 실시하였다. 생산성에서 일당 증체량은 CE1와 CE2 처리구들이 대조구보다 유의적으로 높게 나타내었다(P<0.05). 일당사료섭취량에서는 CE2 처리구가 HC 처리구보다 유의적으로 높게 나타내었고(P<0.05), 대조구에 복합효소제를 첨가할수록 증가하는 Linear 효과를 나타내었다(P<0.05). 사료효율에서는 처리구간의 유의적인 차이를 보이지 않았다(P>0.05). 영양소 소화율에 있어서는 건물, 질소 및 총 에너지 소화율 모두 CE1 처리구가 CON 처리구보다 유의적으로 높게 나타내었고(P<0.05), 대조구에 복합효소제가 첨가될수록 증가하는 Linear 효과를 나타내었다 (P<0.05). 혈액특성에서는 시험기간동안 혈액내 Creatinine과 blood urea nitrogen의 함량에 있어서는 유의적인 차이를 보이지 않았다(P>0.05). 경제성 분석에 있어서는 대조구 사료에 복합효소제 첨가시 기초 사료보다 사료비용이 절감되는 것으로 나타났으며, 특히 복합효소제가 0.05% 첨가된 CE1 처리구에서 사료비용이 절감되었다. 결론적으로 일반적인 육성돈 사료내 0.05% 복합효소제 첨가 급여시 일당증체량, 영양소 소화율 증가되고, 고에너지 사료와 비교하여 150 kcal의 효과와 사료비용을 절감시키는 것으로 알 수 있었다.

• Journal of Animal Science and Technology
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• 제62권2호
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• pp.159-173
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• 2020

A 4×2 factorial feeding trial was designed to investigate the effect of replacing soybean meal (SBM) with cottonseed meal (CSM) in wheat/sorghum/SBM-based diets fed with or without microbial enzymes in diets on the performance, visceral organ development and digestibility of nutrients of broiler chickens. Four graded levels of CSM - none (0%), low (4%, 8%, and 12%), medium (5%, 10%, and 15%), and high (6%, 12%, and 18%) of complete diets in starter, grower and finisher, respectively were fed with or without 100 mg/kg of xylanase and β-glucanase blend. Eight isocaloric and isonitrogenous diets were formulated using least-cost method to meet the nutrient specifications of Ross 308 male broilers. Each treatment was randomly assigned to 6 replicates (10 birds per replicate). There were CSM-enzyme interactions (p < 0.05) on feed intake (FI) and weight gain (WG) in the starter phase. Enzyme supplementation improved (p < 0.05) feed conversion ratio (FCR) in the grower and finisher phases, and increased WG in growing and finishing birds. CSM inclusion reduced (p < 0.05) the weight of gizzard and proventriculus in starter chicks, while these organs were bigger (p < 0.05) in the grower phase. The test ingredient decreased (p < 0.05) small intestinal weight in starter and grower birds. The CSM increased the absolute weight of thighs (p < 0.05) while breast meat was increased (p < 0.01) by enzyme addition. Starch digestibility was improved (p < 0.01) by enzyme inclusion and decreased (p < 0.01) by CSM. Enzyme supplementation improved (p < 0.05) the ileal digestibility of gross energy and protein. The results demonstrate that CSM can substitute up to 90% SBM in broiler chicken diets without compromising performance, and the nutritive value of CSM-containing diets can effectively be improved by enzyme supplementation.