• Proceedings of the Korea Environmental Mutagen Society Conference
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• 2003.05a
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• pp.96-97
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• 2003

• Proceedings of the Korea Environmental Mutagen Society Conference
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• 2004.11a
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• pp.150-150
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• 2004

• Proceedings of the Korean Society of Toxicology Conference
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• 2005.05a
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• pp.158-158
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• 2005

• Environmental Analysis Health and Toxicology
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• v.22 no.4
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• pp.305-312
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• 2007

환경오염을 신속하게 모니터링하기 위한 생물지표의 개발은 증가하고 있는 오염의 심각성에 비추어 매우 중요한 과제로 여겨지고 있다. 본 연구에서는 독성물질처리에 의하여 선택적으로 발현이 조절되는 단백질의 동정을 통하여 독성물질에 대한 단백질 생물지표를 발굴하고자 시도하였다. 즉, 송사리(Oryzias latipes)를 유기인계 살충제인 다이아지논(diazinon)에 0, 0.1, 1, 5 mg/L 농도로 24시간 노출시킨 후, 머리와 몸통부분으로 나누어 단백질 발현패턴을 분석하였다. 본 시스템에서 다이아지논 처리에 의하여 유의적으로 발현이 증가된 단백질로서 alpha-tubulin, ribonuclease pancreatic precursor, protein hfq 등을 동정하였으며, 이 가운데 alpha-tubulin과 $hsp90{\beta}$의 발현이 다이아지논 농도에 의존적으로 증가하는 것을 semi-quantitative RT-PCR방법으로 확인하였다. 이와 같이 다이아지논 처리에 특이적으로 발현이 증가된 송사리 단백질들은 노출평가를 위한 생물지표로서 개발에 응용될 수 있을 것으로 평가된다.

• Proceedings of the Korean Society of Toxicology Conference
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• 2005.05a
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• pp.123-124
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• 2005

• Toxicological Research
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• v.24 no.4
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• pp.235-243
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• 2008

While in some instances, loss of diversity results from acute toxicity (e.g. major pollution incidents), in most cases it results from long-term sub-lethal effects that alter the relative competitive ability and fitness of certain organisms. In such cases the sub-lethal effects will cause a physiological response in the organism that ultimately leads to community level changes. Very sensitive tools are now available to study sub-lethal responses at the molecular level. However, relating such laboratory measurements to ecological effects represents a substantial challenge that can only be met by investigation at all scales (molecular, individual organism and community level) with an appropriate group of organisms. Among the various in vertebrates which can be used as model organisms in such a way, the soil nematode, Caenorhabditis elegans appear to be a promising biological model to diagnose environmental quality. This paper reviews the current status of multilevel biomarkers in environmental toxicology, and C. elegans as promising organisms for this approach.

• Proceedings of the Korean Society of Toxicology Conference
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• 2005.05a
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• pp.197-197
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• 2005

• Proceedings of the Korean Society of Toxicology Conference
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• 2005.05a
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• pp.198-198
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• 2005

• Proceedings of the Korean Society of Toxicology Conference
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• 2005.05a
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• pp.121-121
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• 2005

• Proceedings of the Korea Environmental Mutagen Society Conference
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• 2003.10a
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• pp.149-149
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• 2003