• Proceedings of the Korean Society of Fisheries Technology Conference
• /
• 2002.10a
• /
• pp.216-217
• /
• 2002

Vitellogenin (VTG) is a Precursor form of egg yolk Proteins, which appear only in the blood circulation of female fish and its synthesis in the liver is considered to be regulated by several hormones. It has been reported that in addition to estradiol-17 $\beta$ (E2) several hormones are also involved in the production site of VTG, the liver (Peyon et al., 1996; Mori et al., 1998). (omitted)

• Proceedings of the Botanical Society of Korea Conference
• /
• 1987.07a
• /
• pp.117-132
• /
• 1987

Plants are inherited spatial and temporal coordination systems in their growth and differentiation processes which are precisely governed by the two interlocked control systems; autogenous and environmental. Looking into the overall course of plant development from molecular to organismal level, it can be comparable to a concerto for plant hormones, environmental stimuli and plant genomic orchestra conducted by an unidentified virtuoso. Some of the recent significant attempts to puzzle out the mystery of the life processes of plant development are briefly reviewed. The revolutionary advances in understanding the mystic processes are contemporarily achieved by the application of various molecular techniques. The characterization of plant genomes is now attained through recombinant DNA approaches, and the sensitive detection of specific gene products during the plant development is perimitted by the immunochemical procedures. However, along with the recognition of underlying molecular events such as developmental changes in gene expression and hormone-receptor interrelation associated with tissue sensitivity to hormones, more emphasis should be placed upon the physiological approaches of organismal level for the understanding the correlative systems of the developmental processes of plants as intact eukaryotic organisms.

• Development and Reproduction
• /
• v.25 no.4
• /
• pp.293-298
• /
• 2021

Di(2-ethylhexyl)phthalate (DEHP) could induce metabolic disorders through interfering with thyroid homeostasis. Therefore, we evaluated the effects of short term to environmental relevant doses of DEHP on thyroid hormones. Four week old Sprague-Dawley (SD) rats were treated with vehicle (corn oil), and DEHP 0.75, 7.5, and 150 mg/kg/day. The rats were treated with once daily by oral gavage and were sacrificed with after 1 week. They were measured body weight and relative thyroid weight, serum thyroid hormones and histological changes of thyroid. There was no difference in body weight between the control and DEHP exposed rats. Relative thyroid weight in DEHP 150 mg/kg/day treated group was significantly lower than control. Serum thyroxine levels was decreased in rats exposed to 0.75 and 150 mg/kg/day DEHP. No histological changes were observed in the thyroid of rats administered DEHP compared to control. Exposure to DEHP at environmental relevant levels, even short-term exposure, can cause hypothyroidism in adolescent rats even the exposure period is relative short.

• Toxicological Research
• /
• v.21 no.4
• /
• pp.333-338
• /
• 2005

Many of thyroid hormones disrupting chemicals induce effects via interaction with thyroid hormone and retinoic acid receptors and responsive elements intrinsic in target cells. We studied thyroid hormones disrupting effects of food additives and contaminants including BHA, BHT, ethoxyquin, propionic acid, sorbic acid, benzoic acid, CPM, aflatoxin B1, cadmium chloride, genistein, TCDD, PCBs and TDBE in recombinant HeLa cells containing plasmid construct for thyroxin responsive elements. The limit of response of the recombinant cells to T3 and T4 was $1\times10^{-12}\;M$. BHA. genistein, cadmium and TBDE were interacted with thyroid receptors with dose-responsive pattern. In addition, BHA, BHT, ethoxyquin, propionic acid, benzoic acid, sorbic acid, and TBDE showed synergism while cadmium chloride antagonism for T3-induced activity. This study elucidates that recombinant HeLa cell is sensitive and high-throughput system for the detection of chemicals that induce thyroid hormonal disruption via thyroid hormone receptors and responsive elements. Also this study raised suspect of BHA. BHT, ethoxyquin, propionic acid, benzoic acid, sorbic acid, TBDE, genisteine and cadmium chloride as thyroid hormonal system disruptors.

• Journal of Environmental Science International
• /
• v.2 no.4
• /
• pp.279-289
• /
• 1993

Light qualities and three kinds of plant hormones, NAA, GA3 and BA were treated on maize seedlings to investigate the effect on formation of the chlorophyll-protein complexes. Each three kinds of plant hormones accelerated the chlorophyll proteins formation, particularly LHCP-1 and LHCP-3, but two kinds of hormonal combinations didn't promote these proteins accumulation under sun light condition. The formation of chlorophyll proteins of LHCP-1, CPA and LHCP-3 associated with PSII was promoted under red light compared to sun light, on the contrary the formation of chlorophyll proteins was not affected by white light. Plant hormones under red light induced chlorophyll proteins formation associated with PSII at early state of chloroplast development and two kinds of hormonal combinations under red light were very effective in accumulation of chlorophyll proteins of PSII in contrast to sun light. The results obtained suggest that light may play an important role compared to plant hormones in the formation of chlorophyll proteins.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.38 no.6
• /
• pp.663-666
• /
• 2009

Anti-proliferation effects of Corni fructus ethanol extracts were investigated in the RC58T/h/SA#4 cells treated with environmental hormones including dioxin and bisphenol A. The proliferation was decreased at the concentration over $500{\mu}g/mL$ in the RC58T/h/SA#4 cells with ethanol extracts of various concentrations (1, 10, 100, 500, and $1000{\mu}g/mL$). The environmental hormones such as dioxin and bisphenol A increased the growth of RC58T/h/SA#4 cells in the charcoal-treated FBS (cFBS) medium. The proliferation was the highest at 1 nM and $0.1{\mu}M$ for the tested dioxin and bisphenol A concentration, respectively. Ethanol extracts showed inhibition of the proliferation in a dose-dependent manner at the tested concentrations (10, 100, 300, and $500{\mu}g/mL$) in the RC58T/h/SA#4 cells treated with the environmental hormones. The anti-proliferation was the highest at $500{\mu}g/mL$ concentration among the tested ethanol extracts.

• Journal of Environmental Science International
• /
• v.15 no.12
• /
• pp.1193-1197
• /
• 2006

This study was to examine the variations of chromosome number and the ranges of variety in the suspension culture of ginseng (Panax ginseng C. A. Meyer) callus cell, and the effect of plant hormones for the chromosome aberration. Plant hormones added with MS medium in the suspension culture were 2,4-D, kinetin, and 2,4-D+kinetin and concentration of the plant hormones were $1000{\mu}M$ and $0.1\;{\mu}M$ respectively. As a result of these experiment the following conclusion has been obtained. Media contained with 2,4-D+kinetin in $10{\mu}M$ concentration was very effective in the suspension culture result from 26.4% mitosis frequency, and found the various variation of chromosome number. Variety of chromosome number was diversed ($9\sim110$), espicially frequency of hypohaploid and hyperhaploid cells were very higher than hyperdiploid cells. In this experiments, it is suggested that $10{\mu}M$ 2,4-D+kinetin added with medium in the suspension culture of ginseng callus was effect in the variations of chromosome number.

• Proceedings of the Korea Society of Environmental Biology Conference
• /
• 2002.11a
• /
• pp.33-37
• /
• 2002

The present was performed to identify the effects of tributyltin (TBT) in the immature mice testes. 3-week-old male ICR mice were orally administrated on one time basis of TBT dose of O (Vehicle control, VC), 25 (TBT 25 mg/kg, T$_{25}$ ), 50 (TBT 50 mg/kg, T$_{50}$ ), 100 (TBT 100 mg/kg, T$_{100}$ ) mg/kg per each one. After 3 days the time treated of TBT, mice were sacrified and wighted body, testis, epididymis, seminal vesicle, vas deferens, and prostate. As the result of weighing, wights of each oragan and gonad index were tendency decresed in comparing groups of TBT treated with that (C) of unteated (p <0.05). As the result of examination of steroid hormones in the immature male mice, The concentrations of serum and intratesticular testosterone were significatly increased rather than the control group. But concentrations of estradiol were decresed objectly. A group of the highest change of concentrations of steroid hormones is T$_{100}$ . The high dose group, T$_{100}$ , was decreased all of concentrations of steroid hormones rather than those of T$^{25}$ . The result of observation with histological changes in testis showed a tendency for innercellular wall to increase damage and extinction in seminiferous tubles. As the result of investigation apoptotic cell numbers in the testis using teminal deoxy-nucleotidyl transferase -mediated dUTP-digoxygenin nick end-labeling immunohistochemical straia, The ratio of Apoptic cells significantly was incensed in depending on treatment of TBT does. In conclusion, these results shows that TBT triggers apoptosis on reproductive cell in testis and changes level of concentrations of steroid hormones in the immature male mice , as endocrine disruptors (EDs).

• Korean Journal of Environmental Biology
• /
• v.21 no.4
• /
• pp.352-357
• /
• 2003

Phenanthrene, one of Polycyclic aromatic hydrocarbons with three aromatic rings, is a ubiquitous contaminant in the environment. Phenanthrene has been identified in ambient air, drinking water and sediment. We examined the effect of phenanthrene on steroid hormones level of olive flounder, Paralichthys olivaceus. Plasma testosterone level was increased significantly in fish exposed to phenanthrene($\geq1.0 \mu M$) at 4th week. However, there was no significant changes of estradiol-17$\beta$ concentration in fish exposed to phenanthrene. The physiological variation in phenanthrene exposed fish was a dramatic increase in plasma cortisol level. It is concluded that chronic exposure of phenanthrene can induce increase of plasma testosterone levels and elevate the plasma cortisol level in flounder, Paralichthys olivaceus.

• International Commerce and Information Review
• /
• v.13 no.3
• /
• pp.403-431
• /
• 2011

The environmental issues including domestic measures to protect public life or health are generally easy to bring the tension between the WTO and its member countries. The standard of review, whether de novo review, total deference, or somewhere in between, is largely important in the WTO's adjudicating mechanism because it is closely related to the appropriate balance of power between sovereign nations and the WTO: The multilateral trading regime, through the proper standard of review, could harmoniously operated without interest conflicts among the member countries and at the same time between the WTO and the member countries. Irrespective the important function of the standard of review in the WTO judicial system, applicable standard of review has not been established in the current SPS Agreement. Furthermore, the nature of the SPS Agreement related in scientific factors, such as scientific experiment, data and assessment prevents the WTO's panel from applying consistent standard of review. Considering the judicial demand for the moderate treatment of the case under the SPS Agreement, this paper explores appropriate standard of review applicable the SPS-related environmental measures, particularly, by analysing the recent SPS-related dispute, US-Hormones Suspension.