• Mycobiology
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• v.49 no.5
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• pp.491-497
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• 2021

An endolichenic fungus Xylaria grammica EL000614 produces grammicin, a potent nematicidal pyrone derivative that can serve as a new control option for root-knot nematodes. We optimized an Agrobacterium tumefaciens-mediated transformation (ATMT) protocol for X. grammica to support genetic studies. Transformants were successfully generated after co-cultivation of homogenized young mycelia of X. grammica with A. tumefaciens strain AGL-1 carrying a binary vector that contains the bacterial hygromycin B phosphotransferase (hph) gene and the eGFP gene in T-DNA. The resulting transformants were mitotically stable, and PCR analysis showed the integratin of both genes in the genome of transformants. Expression of eGFP was confirmed via fluorescence microscopy. Southern analysis showed that 131 (78.9%) out of 166 transformants contained a single T-DNA insertion. Crucial factors for producing predominantly single T-DNA transformants include 48 h of co-cultivation, pretreatment of A. tumefaciens cells with acetosyringone before co-cultivation, and using freshly prepared mycelia. The established ATMT protocol offers an efficient tool for random insertional mutagenesis and gene transfer in studying the biology and ecology of X. grammica.

• Korean Journal of Environmental Agriculture
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• v.34 no.1
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• pp.46-51
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• 2015

BACKGROUND: This study aimed at investigating the effects of sulfur-containing compounds widely used as environment-friendly organic fungicides against ginseng anthracnose, and determining the appropriate application concentration for lowering chemical injury to ginseng leaves. Ginseng anthracnose, caused by Colletotrichum gloeosporioides, is a destructive disease that significantly reduces the yield of ginseng. METHODS AND RESULTS: Ginseng anthracnose, caused by Colletotrichum gloeosporioides, is a destructive disease that significantly reduces the yield of ginseng. In a 2-year-old ginseng grown in a pot, treatment with loess-sulfur complex containing 0.06% sulfur and fermented loess-sulfur complex containing 0.13% sulfur did not show any chemical injuries. In order to measure the therapeutic effectiveness, various sulfur-containing compounds were applied to the plants after they were infected with ginseng anthracnose. Treatment with lime sulfur complex (400 dilution) showed the highest ginseng anthracnose control value, followed by fermented loess-sulfur complex (20 dilution), fermented loess-sulfur complex (40 dilution), and loess-sulfur complex (400 dilution) treatments. These compounds were applied before the outbreak of anthracnose disease in order to measure the preventive effectiveness, and in this case, treatment with fermented loess-sulfur complex (40 dilution) showed the highest control value and it was comparable to the value of the pesticide treatment used as the control in this experiment. CONCLUSION: Fermented loess-sulfur complex could be recommended as an environment-friendly organic material to control the occurrence of ginseng anthracnose.

• Research in Plant Disease
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• v.22 no.4
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• pp.227-235
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• 2016

Plant disease caused by root-knot nematode is a major problem in crop production. Using of chemical pesticides, one of the most efficient methods to control nematodes, have raised issues in toxicity to humans and animals and environmental pollution. In this study, to select actinomycete strains that have potential to serve as a microbial agent for control of nematodes, we investigated nematicidal activity of culture broth from 670 Streptomyces isolates. A culture filtrate of KRA15-528 isolate that was identified as S. flavogriseus on the basis of 16S rRNA sequence analysis, showed strong nematicidal activity against second stage of juveniles of Meloidogyne incognita and inhibited egg hatching; exposure to 10% of culture filtrate resulted in 71% juvenile mortality at 48 hours afters treatment and suppressed egg hatching by 54% at 9 days after treatment. When the KRA15-528 culture filtrate was partitioned with ethyl acetate and butanol, ethyl acetate layer exclusively showed strong activity; 91%, 53%, 30% of mortality at 1,000, 500, $250{\mu}g/ml$, respectively. Additionally, the culture filtrate suppressed gall formation on cucumber plant by M. incognita with no phytotoxicity. These results suggest that S. flavogriseus KRA15-528 has potential to serve as a microbial nematicide for the control of root-knot nematode disease.

• Proceedings of the Korea Water Resources Association Conference
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• 2006.05a
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• pp.45-50
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• 2006

About 50% of the population and 75% of the properties concentrate on the flood plain in Japan. The rivers have intimate relationship with our lives. Those conditions have been seen after modern river improvement projects that began about a century ago. The technology which was introduced from foreign countries was improved in conformity with geographical features and the climate condition of our nation, and has redeveloped as a Japanese original technology. In 1940's, Japan had serious natural disasters that were caused by large- scale typhoons. Those typhoons wiped out everything completely. Even though the government realized the importance of flood control and management after those natural disasters, civil work still aimed to economic development. Those construction works have become the one of factors for concentrating population and degrading natural environment in urban areas. Deterioration of river environment has become serious issue in urban development and main cause of pollution. The approaches for environmental restorations which were started about 30 years ago aimed to harmonize with nature environment and cities and human lives. There have been going on many projects called 'river environmental improvement projects', the 'nature friendly river works' and 'natural restoration projects.' The society has tried to find a way to live in harmony with nature. As for societies symbiotic with habitats will form the safe country in the history and the spectacle. Such as the symbiotic of the river or the basin where discharge, water quality, nature, the history, landscape, the local society and also for culture were built in is achieved. Examples of working, applied to restoration technologies and the one to describe the mechanism construction are been shown in the paper. Furthermore, write for an international spread of the river technology of Japan to attempt.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.16 no.1
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• pp.131-145
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• 2013

The purposes of this research are : to analyze biotop type and carry out conservation value assessment in study areas, Daegu Science Park national industrial complex; to supply basic data for the landscape ecological planning; and to improve the application of assessment model with the development of Biotop Value Assessment Tool (B-VAT). The result is as follows. First of all, the field survey showed 434 species of flora and 220 species of insecta. According to the result of biotop type analysis in the biotop classification system with field survey, 13 biotope groups and 63 biotope types were classified. In the map of biotop type classification, readjusted farmland biotop(FA) was prevalent and forest biotop(E) was shown to the southeast side of the site. Next, according to the first assessment with B-VAT, total 19 biotop types including natural river(BA) with abundant plants had I grade and total 16 biotop types such as vegetable garden adjacent to river(BC) had V grade. In terms of the second assessment, we analyzed total 30 areas, total 82 areas, respectively, which had special meaning for the conservation of species and biotop(1a, 1b) and which had meaning for the conservation of species and biotop(2a, 2b, 2c). This research will be a basic data, which can solve the damage problem systematically and control it landscape-friendly with biotop classification and assessment which we developed. In particular, we expect that biotop value assessment tool(B-VAT) with GIS will be a great contribution to popularity compared with the value model by complicated algorism such as adding-matrix, weight and equal distribution. In addition, this will save the time and improve the accuracy for hand-counting.

• Korean Journal of Environmental Agriculture
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• v.31 no.1
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• pp.30-36
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• 2012

BACKGROUND: The resistance of rice bakanae disease pathogens against the fungicide prochloraz has been reported. Understanding the resistance mechanisms is an important for better control of the pathogens. In the present study, we investigated the resistance mechanisms of Fusarium fujikuroi CF337 (CF337) against prochloraz. METHODS AND RESULTS: Morphological changes in the cell wall of CF337 grown in potato dextrose broth (PDB) with or without prochloraz was investigated by transmission electron microscopy. Growth inhibition of CF337 was examined in PDB containing prochloraz or an ABC transporter inhibitor or both of them. Cell wall thickness of CF337 grown in PDB with prochloraz was significantly increased from $80.73{\pm}1.99nm$ to $193.11{\pm}7.07nm$. Significant inhibition in the growth of CF337 was observed in the presence of both prochloraz and the inhibitor, but no growth inhibition was observed in the presence of the inhibitor or prochloraz. Sequence analysis of ATP-binding cassette transporter (ABC) gene of CF337 showed 70 to 80% similarities to the genes of the pathogens resistant to other fungicides. CONCLUSION: Efflux transporter system and changes in cell wall thickness were suggested as resistance mechanisms of CF337 against prochloraz.

• Journal of Adhesion and Interface
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• v.20 no.4
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• pp.140-145
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• 2019

In view of environmental considerations, the control of carbon dioxide (CO₂) and volatile organic compounds (VOCs) is one of important issues in the film and coating industries. Therefore, UV-curable system has been developed due to minimize emissions of VOCs and reduce CO₂ emission due to low energy consumption from fast curing. Also, biodegradable polymers economically are attractive because of environmental and economic concerns associated with huge waste plastics. In this study, UV-curable polyurethane acrylates with different compositions of biodegradable polylactide (PLA) diol and poly(ethylene glycol) as diols were synthesized and curing reaction of their end-capped acrylates was performed by UV exposure. Tensile strength, elongation, and Tg of the UV-cured polyurethane acrylates increased with PLA diol content in the diol while their hydrophilicity and thermal stability increased with the PEG content. These results indicated a property of UV-cured polyurethane acrylates could be controlled by environment-friendly diols.

• Journal of the Korean Recycled Construction Resources Institute
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• v.11 no.4
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• pp.501-508
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• 2023

Addressing urban noise problems, this study develops eco-friendly, inorganic sound-absorbing panels, overcoming the limitations of traditional PMMA and cement-based panels. These conventional panels pose safety risks due to flammability and environmental concerns due to carbon emissions. Utilizing industrial waste, the research comprises two phases: initial tests for physical and performance characteristics (fluidity, density, compressive strength, sound absorption) and subsequent development of optimized panel mixtures. This approach aims to replace existing panels with sustainable, effective alternatives, significantly contributing to safer, environmentally responsible urban infrastructure. The findings of this study have implications for the sound panel market, offering novel solutions for noise control while aligning with environmental and safety standards.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.6
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• pp.2789-2796
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• 2012

The purpose of this study was to develope the Contemporary Han-ok based on the natural environment characteristics of mountainous territory in Gangwon-do. According to the result of this study, Eco-friendly Contemporary Han-ok adaptable to the natural environment feature focused on the local climate was proposed based on the tradition of mountainous territory housing in Gangwon-do. In addition, proposed Mountainous Han-ok in Gangwon-do planned to keep the out feature of traditional Korean Han-ok and have the environmental control system same as traditional Korean Han-ok. Despite of several limits, this study has the meaning in terms of propose the prototype of adaptable contemporary han-ok based on the Natural Environment Characteristics of Mountainous Territory and regional tradition in Gangwon-do in cognition of local natural environment difference. the proposal of contemporary han-ok as the result of this study will give a support to the wide planning of Han-ok and the development plan of contemporary Han-ok.

• Journal of Institute of Control, Robotics and Systems
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• v.21 no.5
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• pp.447-452
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• 2015

A hybrid power system was developed for agricultural machines with a 20kW output capacity, and it was attached to a multi-purpose cultivator to improve the performance of the cultivator. The hybrid system combined heterogeneous sources: an internal-combustion engine and an electric power motor. In addition, a power splitter was developed to simplify the power transmission structure. The cultivator using a hybrid system was designed to have increased fuel efficiency and output power and reduced exhaust gas emissions, while maintaining the functions of existing cultivators. The fuel consumption for driving the cultivator in the hybrid engine vehicle (HEV) mode was 341g/kWh, which was 36% less than the consumption in the engine (ENG) mode for the same load. The maximum power take off output of the hybrid power system was 12.7kW, which was 38% more than the output of the internal-combustion engine. In the HEV mode, harmful exhaust gas emissions were reduced; i.e., CO emissions were reduced by 36~41% and NOx emissions were reduced by 27~51% compared to the corresponding emissions in the ENG mode. The hybrid power system improved the fuel efficiency and reduced exhaust gas emissions in agricultural machinery. The hybrid system's lower exhaust gas emissions have considerable advantages in closed work environments such as crop production facilities. Therefore, agricultural machinery with less exhaust gas emissions should be commercialized.