• Journal of Oral Medicine and Pain
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• v.36 no.3
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• pp.147-160
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• 2011

Eugenol (4-allyl-2-methoxyphenol) is a natural phenolic constituent extensively used in dentistry as a component of zinc oxide eugenol cement and is applied to the mouth environment. Chios gum mastic (CGM) is a resinous exudate obtained from the stem and the main leaves of Pistacia lenticulus tree native to Mediterranean areas. This study was undertaken to investigate the synergistic apoptotic effect of co-treatment with a natural product, CGM and natural phenolic compound, eugenol on SCC25 human tongue squamous cell carcinoma cell line. To investigate whether the co-treatment with eugenol and CGM compared to each single treatment efficiently reduces the viability of SCC25 cells, MTT assay was conducted. Induction and augmentation of apoptosis were confirmed by Hoechst staining, TUNEL staining and DNA hypoploidy. Westen blot analysis and immunofluorescent staining were performed to study the alterations of the expression level and the translocation of apoptosis-related proteins in co-treatment. In this study, co-treatment of with eugenol and CGM on SCC25 cells showed several lines of apoptotic manifestation such as nuclear condensations, DNA fragmentation, the increase and decrease of Bax and Bcl-2, decrease of DNA content, the release of cytochrome c into cytosol, translocation of AIF and DFF40 (CAD) onto nuclei, and activation of caspase-3, caspase-6 caspase-7, caspase-9, PARP, Lamin A/C and DFF45 (ICAD) whereas each single treated SCC25 cells did not show or very slightly these patterns. Although the single treatment of 40 ${\mu}g$/ml CGM and 0.5 mM eugenol for 24 h did not induce apoptosis, the co-treatment of these reagents prominently induced apoptosis. Therefore our data provide the possibility that combination therapy with CGM and eugenol could be considered as a novel therapeutic strategy for human oral squamous cell carcinoma.

• Journal of Fashion Business
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• v.3 no.4
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• pp.139-146
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• 1999

The purpose of this study is to analyze the efficiency and working conditions of the patternwork departments by surveying the apparel manufacturing companies sampled in Seoul for their organization, working enviornment and technology of their patternwork departments, and thereby, find the ways to promote the functions of the patternwork department. The results of this study can be summarized as follows; 1. As a result of analyzing the working environment of patternwork departments, it was found that the problems counted by most of the pattern workers were lack of workforce, unhygienic or unpleasant workplaces. 2. As a consequence of analyzing the sample production in terms of tacking methods and times, They rarely tacked the white cotton. Such an omission of tacking procedure may serve to save time, but if a sewing error occurs, the working time would rather be longer due to the defects of pattern to be remedied. 3. As a result of reviewing the general patternwork conditions, it was found that the patterns were still worked out in two dimensions. On the other hand, more than 60% of the pattern workers were applying to their patternworks the styles which they had learned from their seniors. Few patterns are being developed in conformity to apparel trends or body shape changes. 4. As a consequence of examining the problems involving the production of patternsl, the problems involved primarily the patterns incised off no-dart pattern and body structure, and in light of items, involved mainly the trousers and in light of material, involved much the stretch or velvet. Moreover, it is deemed necessary to establish a special institute devoted to a systematic development of pattern technology. 5. Most of the CAD patternworks are used not for pattern designing but for grading and marking works, which suggests that pattern workers need to be educated on this new technology for more diverse and precise patternworks. 6. Lastly, as a result of examining the conditions of patternwork departments and their relevant works, most of the pattern workers were found to be subject to irregular and excessive workload, having little time to produce good patterns. This finding suggests that it is necessary to mandate the pattern workers to more involve the commodity planning.

• Korean Journal of Veterinary Service
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• v.37 no.2
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• pp.85-96
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• 2014

Total 99 strains of Campylobacter spp. were isolated from 117 cases of duck's fecal samples. Among 99 strains of Campylobacter spp. isolates, 93 strains (93.9%) were C. jejuni and 6 strains (6.1%) were C. coli. Prevalence of virulence and GBS associated genes of 72 C. jejuni isolates was determined by m-PCR. Among the 10 kinds of virulence associated genes, cadF, dnaJ, flaA and ceuE genes were detected in all of C. jejuni isolates from ducks, racR, pldA, iamA, ciaB, virB11 and docC genes were 87.5%, 84.7%, 77.8%, 48.6%, 13.9% and 11.1%, respectively. Antimicrobial susceptibility test was performed on 72 C. jejuni isolates. The rate of resistance were 62.5% for oxytetracycline, 55.6% for kanamycin, 54.2% for enrofloxacin, 50% for ciprofloxacin, 37.5% for tetracycline and nalidixic acid, 18.1% for ampicillin, 15.3% for streptomycin, and 6.9% for ofloxacin. All isolates were susceptible to erythromycin. The adherence (intracellular and extracellular bacteria) abilities of the 20 isolates to INT-407 cells were between $4.21{\pm}1.27{\times}10^4$ CFU/well and $1.053{\pm}0.451{\times}10^6$ CFU/well from the isolates of cj-55 and cj-52, respectively, and that can be expressed as 0.1033% to 5.2655% to the infecting inoculum. The invasion (intracellular bacteria) abilities of the 20 isolates to INT-407 were between $1.00{\pm}1.73{\times}10^3$ CFU/well and $8.47{\pm}5.16{\times}10^4$ CFU/well from the isolates of cj-13 and cj-47, respectively, and that can be expressed as 0.0050% to 0.4235% to the infecting inoculums. The average CFU/well of 20 campylobacters isolated from ducks for adherence to and invasion were $2.646{\pm}2.886{\times}10^5$ and $3.03{\pm}2.7{\times}10^4$ respectively, and that was $1.3230{\pm}1.2139%$ and $0.1516{\pm}0.1343%$ of the starting viable inoculum. There was considerable correlation ($R^2$=0.627) between the adherence and invasion ability of C. jejuni isolates for INT-407 cell.

• Journal of the Korean Society of Clothing and Textiles
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• v.41 no.2
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• pp.242-253
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• 2017

We selected 81 universities (39.1%) for research and close analysis to analyze the current availability of courses in the field of clothing construction at four-year domestic universities. The results of the analysis shows that curriculum is currently composed of theoretical and practical courses that continue to be developed in diverse areas of study. Despite having classes with underlying similarities and differences, research shows that the course curriculum is constructed systematically with both theory and practice at each stage. Distribution of curriculum in the field of clothing construction were organized and analyzed by similar courses in 4 larger groupings and 9 sub-groups. Analysis of the large groupings showed that sewing courses (19.8%), pattern development courses (41.0%), advanced sewing courses (14.1%), and miscellaneous courses (26.1%) were established. Analysis of the sub-groups showed that basic theory and sewing courses (3.6%), sewing courses (16.1%), flat pattern courses (17.9%), draping pattern courses (14.6%), pattern CAD courses (7.3%), tailoring and advanced sewing courses (8.5%), scientific sewing and apparel production processing courses (1.4%), special sewing courses (3.9%), and miscellaneous and new subject courses (26.1%) were established. Overall analysis of the organization distribution was conducted in the order of pattern development (39.9%), miscellaneous & new subject (26.1%), basic theory and sewing (19.8%), and advanced sewing (14.0%). Field analysis from various angles indicates that pattern development, new subject courses, and miscellaneous courses had a relatively higher proportion than other major courses. From this it can be inferred that this trend is an effort to develop individuals to adapt to a rapidly changing fashion industry and environment. The composition of courses also shows great advancement towards diversification and course development. However, there is still insufficient exchange between industrial and education sites. As active exchange continue to be demanded, classification standards for new subject courses and titles of such studies have become unclear and have reached limitations.

• Molecules and Cells
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• v.40 no.4
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• pp.299-306
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• 2017

The transcriptional activator AphB has been implicated in acid resistance and pathogenesis in the food borne pathogens Vibrio vulnificus and Vibrio cholerae. To date, the full-length AphB crystal structure of V. cholerae has been determined and characterized by a tetrameric assembly of AphB consisting of a DNA binding domain and a regulatory domain (RD). Although acidic pH and low oxygen tension might be involved in the activation of AphB, it remains unknown which ligand or stimulus activates AphB at the molecular level. In this study, we determine the crystal structure of the AphB RD from V. vulnificus under aerobic conditions without modification at the conserved cysteine residue of the RD, even in the presence of the oxidizing agent cumene hydroperoxide. A cysteine to serine amino acid residue mutant RD protein further confirmed that the cysteine residue is not involved in sensing oxidative stress in vitro. Interestingly, an unidentified small molecule was observed in the inter-subdomain cavity in the RD when the crystal was incubated with cumene hydroperoxide molecules, suggesting a new ligand-binding site. In addition, we confirmed the role of AphB in acid tolerance by observing an aphB-dependent increase in cadC transcript level when V. vulnificus was exposed to acidic pH. Our study contributes to the understanding of the AphB molecular mechanism in the process of recognizing the host environment.

• Journal of Technologic Dentistry
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• v.36 no.4
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• pp.277-296
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• 2014

Purpose: This study intends to set standard for organizing the curriculum of short-term dental technicians and the change of job environment, as well as the knowledge & training, by identifying the importance and actual job performance of dental technicians following job analysis in the university education of Dept. of Dental Laboratory Technology, and furthermore to foster junior executives to cope with modernization and globalization. Methods: The tools used in this study are based on the job analysis of research report suggested by National Health Personnel Licensing Examination Board(Lee Gyu-seon, 2011), and it consists of general characteristic 7 items. Every competence duty was measured through Likert 5 point gauging, and internal consistency through Cronbach's Alpha. The competence importance of entire questionnaire was .984 and its performance was .874, reflecting considerably high level, and in terms of each competence duty, high credibility was proved and high internal consistency was verified with competence importance ranging from the minimum .655 to the maximum .966 and its performance ranging from the minimum .677 to the maximum .993. Results: Competence importance and its performance in each duty on the job analysis of dental technicians were measured. In competence importance in each duty, the highest was "checking design order form"(average, 4.52), followed by "selling dental prosthesis"(average 4.49), "making removable orthodontic appliance"(average, 4.48), "open managing dental laboratory"(average, 4.46) in order. However, "making complete denture" was found the lowest(average, 4.23), with importance of all competence dutys was considerably high level. In the performance of its competence duty, "checking design order form"(average, 4.04) was found to be the most importance competence, followed by "making conservative restoration"(average, 4.00), "making porcelain"(average 3.98), "checking working cast"(average, 3.90) in order. However it was found out that "making fixed orthodontic appliance"(average, 3.12) was the lowest, and the importance of all competence dutys was quite high level. Conclusion: The duties of dental technicians consist of 13 kinds, in total, and it was found out that "checking design order form"(average, 4.52) was the most important in the competence importance and job performance. In particular, it was revealed that there was a great difference between the competence importance and its performance in the order of "selling dental prosthesis", "open managing dental laboratory", "making removable orthodontic appliance", "making CAD/CAM prosthesis", and then "making fixed orthodontic appliance".

• The Journal of Korean Academy of Prosthodontics
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• v.56 no.4
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• pp.338-346
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• 2018

Crossed occlusion can be treated either by overdenture and telescopic denture or by placing an implant at the edentulous area to reestablish the support on the occlusion. If alveolar bony support is sufficient and an the environment where an implant is inserted is favorable to restoring the masticatory and aesthetic function of a patient, the implant-supported fixed prosthesis can provide more definitive occlusal support and more aid for other oral functions. In this case report, a patient with a severe residual alveolar bone resorption following the extraction of teeth and who had a crossed occlusion was treated with sinus bone graft and alveolar bone augmentation in order to place the implants at prosthetically position. The definitive restoration was made to reflect the patient's occlusal and aesthetic function using the CAD/CAM double scanning method. Finally, the treatment had the masticatory and aesthetic function adequately restored, which is reported here.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.5
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• pp.1573-1579
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• 2010

In this paper, we studied efficient design of wireless transmission system for unified data transmission in a subway and railway. It is increased that need of broadband multimedia service to make useful environment for users and to support the operation of railway system. High bandwidth is better if we need more services. But, high bandwidth requires more cost at tunnel of subway. And more bandwidth makes received antenna sensitivity bad. So it needs more wireless stations. We deduced best bandwidth for subway wireless transmission system using the cost of installation and efficiency of system. Consequently, we proposed efficient service model for broadband wireless system at a subway. Subway broadband wireless transmission system is testing and extended to province subway. The cost of subway broadband wireless transmission system is saved, because the system can be efficiently designed using proposed service model. Therefore, the effectiveness of it will be expected to be very big.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.11
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• pp.7667-7671
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• 2015

Recently, Zinc coating is often used with environment friendly features and high performance. Generally The coating temperature is one of main factors for determining coating thickness and coating ability, so the optimal coating temperature is strongly required. In this paper, the thermo-flow simulation considering the air flow inside the coating rooms for analyzing the temperature distributions of Zinc spray coating room was performed. Two spray coating rooms, preheating room and drying room were all modeled by SolidWorks program and the temperature distributions were analyzed by Flow simulation program. The analysis results were verified with the measured data by thermal image camera. The characteristics of temperature distributions of the first spray room and the second spray room were understood and the results showed that the temperatures of two spray coating room were low compared with the target temperature $25^{\circ}C$. To the exclusion of heater addition, the simulation with all the same conditions exclusive the exhaust fan was performed, which showed that the temperatures of the first and the second spray rooms increased by $6.2^{\circ}C$ and $5.8^{\circ}C$. This analysis can be applicable for designing a new spray coating room for improving performance.

• Proceedings of the Korean Institute Of Construction Engineering and Management
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• 2008.11a
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• pp.170-175
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• 2008

The research team for the virtual construction development was established with the support of Korea Ministry of Construction and Transportation, and KICTEP (Korea Institute of Construction and Transportation Technology Evaluation and Planning). Its aims are to develop system that is to improve productivity & quality, to create a higher value-added business, and to cultivate international competitiveness in the construction industry. The virtual construction system is a design, engineering, and construction management information system that allows the project participants to effectively share the information throughout the construction life cycle with the support of 3D and design information. To achieve this, the research team focuses on developing several systems. First, the team focuses on developing for the pre-planning, the structural engineering, MEP, and the 3D based estimation system. Second, they focus on developing a simulation system for the construction process planning and feasibility study with help of the virtual reality technologies. Third, they focus on developing the CPLM (Construction Project Life-cycle Management) system for managing construction project data, and the decision support system that makes the collaboration among the project participants based on 3D technologies and information. We also focus on developing the SDAI (Standard Data Access Interface), the localized guideline for 3D design, and a training program. In addition, we focus on developing the undeveloped area of the commercial system and building an environment that can support the communication and collaboration in the construction life-cycle rather than developing the existing and commercialized system.