Crystal Structure of the Regulatory Domain of AphB from Vibrio vulnificus, a Virulence Gene Regulator |
Park, Nohra
(Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, and Research Institute for Agriculture and Life Sciences, Seoul National University)
Song, Saemee (Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, and Research Institute for Agriculture and Life Sciences, Seoul National University) Choi, Garam (Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, and Research Institute for Agriculture and Life Sciences, Seoul National University) Jang, Kyung Ku (Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, and Research Institute for Agriculture and Life Sciences, Seoul National University) Jo, Inseong (Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, and Research Institute for Agriculture and Life Sciences, Seoul National University) Choi, Sang Ho (Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, and Research Institute for Agriculture and Life Sciences, Seoul National University) Ha, Nam-Chul (Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, and Research Institute for Agriculture and Life Sciences, Seoul National University) |
1 | Adams, P.D., Afonine, P.V., Bunkoczi, G., Chen, V.B., Davis, I.W., Echols, N., Headd, J.J., Hung, L.W., Kapral, G.J., Grosse-Kunstleve, R.W., et al. (2010). PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta Crystallogr. D Biol. Crystallogr. 66, 213-221. DOI |
2 | Horseman, M.A., and Surani, S. (2011). A comprehensive review of Vibrio vulnificus: an important cause of severe sepsis and skin and soft-tissue infection. Int. J. Infect Dis. 15, e157-166. DOI |
3 | Jang, K.K., Gil, S.Y., Lim, J.G., and Choi, S.H. (2016). Regulatory Characteristics of Vibrio vulnificus gbpA Gene Encoding a Mucin-binding Protein Essential for Pathogenesis. J. Biol. Chem. 291, 5774-5787. DOI |
4 | Jeong, H.G., and Choi, S.H. (2008). Evidence that AphB, essential for the virulence of Vibrio vulnificus, is a global regulator. J. Bacteriol. 190, 3768-3773. DOI |
5 | Kantardjieff, K.A., and Rupp, B. (2003). Matthews coefficient probabilities: Improved estimates for unit cell contents of proteins, DNA, and protein-nucleic acid complex crystals. Protein Sci. 12, 1865-1871. DOI |
6 | Kovacikova, G., Lin, W., and Skorupski, K. (2010). The LysR-type virulence activator AphB regulates the expression of genes in Vibrio cholerae in response to low pH and anaerobiosis. J. Bacteriol. 192, 4181-4191. DOI |
7 | Kovacikova, G., and Skorupski, K. (1999). A Vibrio cholerae LysR homolog, AphB, cooperates with AphA at the tcpPH promoter to activate expression of the ToxR virulence cascade. J. Bacteriol. 181, 4250-4256. |
8 | Krukonis, E.S., Yu, R.R., and Dirita, V.J. (2000). The Vibrio cholerae ToxR/TcpP/ToxT virulence cascade: distinct roles for two membranelocalized transcriptional activators on a single promoter. Mol. Microbiol. 38, 67-84. DOI |
9 | Lee, M.A., Kim, J.A., Yang, Y.J., Shin, M.Y., Park, S.J., and Lee, K.H. (2014). VvpM, an extracellular metalloprotease of Vibrio vulnificus, induces apoptotic death of human cells. J. Microbiol. 52, 1036-1043. DOI |
10 | Lim, J.G., Park, J.H., and Choi, S.H. (2014). Low cell density regulator AphA upregulates the expression of Vibrio vulnificus iscR gene encoding the Fe-S cluster regulator IscR. J. Microbiol. 52, 413-421. DOI |
11 | Milton, D. L., O'Toole, R., Horstedt, P., and Wolf-Watz, H. (1996). Flagellin A is essential for the virulence of Vibrio anguillarum. J. Bacteriol. 178, 1310-1319 DOI |
12 | Liu, Z., Yang, M., Peterfreund, G.L., Tsou, A.M., Selamoglu, N., Daldal, F., Zhong, Z., Kan, B., and Zhu, J. (2011). Vibrio cholerae anaerobic induction of virulence gene expression is controlled by thiol-based switches of virulence regulator AphB. Proc. Natl. Acad. Sci. USA 108, 810-815. DOI |
13 | Liu, Z., Wang, H., Zhou, Z., Naseer, N., Xiang, F., Kan, B., Goulian, M., and Zhu, J. (2016) Differential Thiol-Based Switches Jump-Start Vibrio cholerae Pathogenesis. Cell Rep. 14, 347-354. DOI |
14 | Merrell, D.S., and Camilli, A. (2000). Regulation of vibrio cholerae genes required for acid tolerance by a member of the "ToxR-like" family of transcriptional regulators. J. Bacteriol. 182, 5342-5350. DOI |
15 | Park, S., Ha, S., and Kim, Y. (2017) The protein crystallography beamlines at the pohang light source ll. BIODESIGN 5, 30-34. |
16 | Miroux, B., and Walker, J.E. (1996). Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. J. Mol. Biol. 260, 289-298. DOI |
17 | Otwinowski, Z., and Minor, W. (1997). Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol. 276, 307-326. |
18 | Park, J.H., Jo, Y., Jang, S.Y., Kwon, H., Irie, Y., Parsek, M.R., Kim, M.H., and Choi, S.H. (2015). The cabABC operon essential for biofilm and rugose colony development in vibrio vulnificus. PLoS Pathogens 11, e1005252. DOI |
19 | Patel, V.P., Rojas, M.R., Paplomatas, E.J., and Gilbertson, R.L. (1993). Cloning biologically active geminivirus DNA using PCR and overlapping primers. Nucleic Acids Res. 21, 1325-1326. DOI |
20 | Rhee, J.E., Kim, K.S., and Choi, S.H. (2005). CadC activates pHdependent expression of the Vibrio vulnificus cadBA operon at a distance through direct binding to an upstream region. J. Bacteriol. 187, 7870-7875. DOI |
21 | Rhee, J.E., Jeong, H.G., Lee, J.H., and Choi, S.H. (2006). AphB influences acid tolerance of Vibrio vulnificus by activating expression of the positive regulator CadC. J. Bacteriol. 188, 6490-6497. DOI |
22 | Simon, R., Priefer, U., and Phuler, A. (1983). A broad host range mobilization system for invivo genetic-engineering - transposon mutagenesis in gram-negative bacteria. Bio-Technol. 1, 784-791. DOI |
23 | Taylor, J.L., De Silva, R.S., Kovacikova, G., Lin, W., Taylor, R.K., Skorupski, K., and Kull, F.J. (2012). The crystal structure of AphB, a virulence gene activator from Vibrio cholerae, reveals residues that influence its response to oxygen and pH. Mol. Microbiol. 83, 457-470. DOI |
24 | Wright, A. C., Simpson, L. M., Oliver, J. D., and Morris, J. G. (1990). Phenotypic evaluation of acapsular transposon mutants of Vibrio vulnificus. Infect. Immun. 58, 1769-1773 |
25 | Winn, M.D., Ballard, C.C., Cowtan, K.D., Dodson, E.J., Emsley, P., Evans, P.R., Keegan, R.M., Krissinel, E.B., Leslie, A.G., McCoy, A., et al. (2011). Overview of the CCP4 suite and current developments. Acta Crystallogr. D Biol. Crystallogr. 67, 235-242. DOI |
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